Rh4CG237400

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007552
Physical Location & Seq
Forward (+)
48188705 .. 48193946
5242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG237400.1

Sequence Viewer

Length: 798 bp
ATGGGGAGCAGGTTATGCTGCTTTCACTCTTTGGAGTTGTATTCAGCTTTCATTGAATATTGCTTTGTATTGTGTGTCTATGAAAATGTTAAGGAATTTATGTTGACGCAGGAGAAGAAAGCCGTCAAGCAAGAGGACTTGAAACAACAGCTGAAGTATAAGTGGGCTCTCGTTGATGGTATCAGAAAAGAGTCAGGAAAGCTTGTAAGGCGGATTTCTCCAGAAGATGTTACAATAAATACAGGAAAGGATGCCCTGATTCCAGGATATCCAATCCCTGGTGAAAGCTGGAAAGAAATAAGGCATGACAATACTGTGACATGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGAAGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTTGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTAGAACTGAGAAGGTTGATGAGGATGATGATGAAGCTGATATTGTTAGTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTGTTAGCGTCGAGCAAGGGGACTATAGAGGGAGATGTTTCAGAGAAACTTGATGTTTATGCCCGTGCAAACAAGGAGGTTGCTATCACTTGTAATCATCTGTGCAGTATCTCAAAGACTGATAGTGAACAAACCTCTAGGTTAATGCTGAAGATTTACAAGCTCCTGGACATTTTGACAGAGCTGAAAGCATACTTGGACGGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

265

Amino Acids

30.2

Weight (kDa)

8.0

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 65 - 108 3.7e-11 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 110 - 194 8.3e-21 Eukaryotic DNA topoisomerase I, catalytic core
Topoisom_I PF01028 206 - 260 8.2e-07 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 278
AciI CCGC 3 cut(s) 211, 445, 547
AcsI RAATTY 2 cut(s) 95, 356
AcuI CTGAAG 2 cut(s) 173, 758
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 2 cut(s) 278, 336
AflIII ACRYGT 1 cut(s) 320
AgsI TTSAA 4 cut(s) 56, 142, 334, 379
AjnI CCWGG 3 cut(s) 262, 277, 753
AjuI GAANNNNNNNTTGG 1 cut(s) 767
AluBI AGCT 9 cut(s) 47, 151, 202, 288, 395, 451, 530, 751, 772
AluI AGCT 9 cut(s) 47, 151, 202, 288, 395, 451, 530, 751, 772
ApeKI GCWGC 3 cut(s) 18, 404, 448
ApoI RAATTY 2 cut(s) 95, 356
AsuHPI GGTGA 1 cut(s) 293
AsuNHI GCTAGC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 169
BbvI GCAGC 3 cut(s) 5, 391, 460
BccI CCATC 2 cut(s) 170, 428
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 3 cut(s) 264, 279, 755
BfaI CTAG 3 cut(s) 452, 495, 726
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 5 cut(s) 19, 405, 446, 449, 547
BlsI GCNGC 5 cut(s) 20, 406, 447, 450, 548
Bme1390I CCNGG 3 cut(s) 264, 279, 755
BmrFI CCNGG 3 cut(s) 264, 279, 755
BmsI GCATC 1 cut(s) 241
BmtI GCTAGC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 336
BseBI CCWGG 3 cut(s) 264, 279, 755
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 3 cut(s) 256, 439, 523
BseLI CCNNNNNNNGG 2 cut(s) 278, 336
BseMII CTCAG 2 cut(s) 479, 492
BseXI GCAGC 3 cut(s) 5, 391, 460
BsgI GTGCAG 1 cut(s) 712
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 2 cut(s) 278, 336
BsmFI GGGAC 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 169
BspACI CCGC 3 cut(s) 211, 445, 547
BspCNI CTCAG 2 cut(s) 480, 493
BspOI GCTAGC 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 277
Bst2UI CCWGG 3 cut(s) 264, 279, 755
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 2 cut(s) 488, 501
BstENI CCTNNNNNAGG 1 cut(s) 334
BstF5I GGATG 3 cut(s) 256, 439, 523
BstMWI GCNNNNNNNGC 3 cut(s) 15, 208, 401
BstNI CCWGG 3 cut(s) 264, 279, 755
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 3 cut(s) 262, 277, 753
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 5, 391, 460
BtsCI GGATG 3 cut(s) 256, 439, 523
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 453
CseI GACGC 2 cut(s) 115, 585
Csp6I GTAC 1 cut(s) 324
CviAII CATG 3 cut(s) 305, 321, 472
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 2 cut(s) 488, 501
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
EciI GGCGGA 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 269
Eco57I CTGAAG 2 cut(s) 173, 758
EcoNI CCTNNNNNAGG 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 262, 277, 753
EcoRV GATATC 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 3 cut(s) 308, 324, 475
FaqI GGGAC 1 cut(s) 622
FatI CATG 3 cut(s) 304, 320, 471
Fnu4HI GCNGC 5 cut(s) 19, 405, 446, 449, 547
FokI GGATG 3 cut(s) 263, 446, 530
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 5 cut(s) 19, 405, 446, 449, 547
FspBI CTAG 3 cut(s) 452, 495, 726
GluI GCNGC 5 cut(s) 19, 405, 446, 449, 547
GsuI CTGGAG 1 cut(s) 204
HgaI GACGC 2 cut(s) 115, 585
Hin1II CATG 3 cut(s) 308, 324, 475
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 2 cut(s) 191, 259
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 105, 716
Hpy188I TCNGA 3 cut(s) 185, 489, 631
Hpy188III TCNNGA 2 cut(s) 195, 221
Hpy8I GTNNAC 2 cut(s) 105, 716
Hpy99I CGWCG 1 cut(s) 601
HpyAV CCTTC 2 cut(s) 373, 499
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 3 cut(s) 407, 656, 693
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 208, 401
HpyF3I CTNAG 2 cut(s) 488, 501
Hsp92II CATG 3 cut(s) 308, 324, 475
LmnI GCTCC 3 cut(s) 6, 392, 756
Lsp1109I GCAGC 3 cut(s) 5, 391, 460
LweI GCATC 1 cut(s) 241
MaeI CTAG 3 cut(s) 452, 495, 726
MaeIII GTNAC 3 cut(s) 229, 316, 344
MboII GAAGA 4 cut(s) 127, 236, 346, 751
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 95, 356
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 8 cut(s) 127, 330, 424, 483, 508, 610, 658, 733
MseI TTAA 4 cut(s) 90, 554, 731, 796
MspA1I CMGCKG 1 cut(s) 151
MspR9I CCNGG 3 cut(s) 264, 279, 755
MvaI CCWGG 3 cut(s) 264, 279, 755
MwoI GCNNNNNNNGC 3 cut(s) 15, 208, 401
NheI GCTAGC 1 cut(s) 451
NlaIII CATG 3 cut(s) 308, 324, 475
NmuCI GTSAC 2 cut(s) 316, 344
NspI RCATGY 1 cut(s) 324
PciI ACATGT 1 cut(s) 320
PfeI GAWTC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 278
PfoI TCCNGGA 2 cut(s) 262, 753
PkrI GCNGC 5 cut(s) 20, 406, 447, 450, 548
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PscI ACATGT 1 cut(s) 320
Psp6I CCWGG 3 cut(s) 262, 277, 753
PspGI CCWGG 3 cut(s) 262, 277, 753
PvuII CAGCTG 1 cut(s) 151
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
SaqAI TTAA 4 cut(s) 90, 554, 731, 796
SatI GCNGC 5 cut(s) 19, 405, 446, 449, 547
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 264, 279, 755
SduI GDGCHC 1 cut(s) 169
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 612
Sse9I AATT 2 cut(s) 95, 356
SsiI CCGC 3 cut(s) 211, 445, 547
SspI AATATT 1 cut(s) 59
SspMI CTAG 3 cut(s) 452, 495, 726
StyD4I CCNGG 3 cut(s) 262, 277, 753
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 599
TasI AATT 2 cut(s) 95, 356
TatI WGTACW 1 cut(s) 323
TauI GCSGC 2 cut(s) 448, 549
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 4 cut(s) 90, 554, 731, 796
Tru9I TTAA 4 cut(s) 90, 554, 731, 796
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 2 cut(s) 316, 344
TseI GCWGC 3 cut(s) 18, 404, 448
Tsp45I GTSAC 2 cut(s) 316, 344
TspDTI ATGAA 3 cut(s) 40, 96, 540
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 278
XagI CCTNNNNNAGG 1 cut(s) 334
XapI RAATTY 2 cut(s) 95, 356
XceI RCATGY 1 cut(s) 324
XspI CTAG 3 cut(s) 452, 495, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.