Rh2BG558800

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007552
Physical Location & Seq
Reverse (-)
76947358 .. 76949520
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG558800.1

Sequence Viewer

Length: 513 bp
ATGGTGGATTCTCTGTTCCGAAGAGACATGCTGAGGGGAGTGCTTCCCATTCTCTGTTCAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGATTACATAAGAGCTATCAGGGCTGCATACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGGTTTGTAGTCATCTGCGTGATATCGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATATCATCTCTAATCATAAGAGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

170

Amino Acids

19.27

Weight (kDa)

5.33

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 24 - 91 2.8e-20 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 190
AclWI GGATC 2 cut(s) 450, 463
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 398, 488
AfaI GTAC 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 81
AgsI TTSAA 4 cut(s) 79, 124, 286, 332
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 5 cut(s) 140, 196, 412, 480, 502
AluI AGCT 5 cut(s) 140, 196, 412, 480, 502
Alw21I GWGCWC 2 cut(s) 379, 469
Alw26I GTCTC 1 cut(s) 18
Alw44I GTGCAC 2 cut(s) 375, 465
AlwI GGATC 2 cut(s) 450, 463
Ama87I CYCGRG 1 cut(s) 159
ApaLI GTGCAC 2 cut(s) 375, 465
ApeKI GCWGC 2 cut(s) 149, 193
ApoI RAATTY 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 317, 419, 509
AsuNHI GCTAGC 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 159
BaeGI GKGCMC 2 cut(s) 379, 469
BamHI GGATCC 1 cut(s) 455
Bbv12I GWGCWC 2 cut(s) 379, 469
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 136, 205
BccI CCATC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 2 cut(s) 197, 387
BfmI CTRYAG 1 cut(s) 460
BisI GCNGC 3 cut(s) 150, 191, 194
BlsI GCNGC 3 cut(s) 151, 192, 195
BmcAI AGTACT 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 457
BmtI GCTAGC 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 2 cut(s) 184, 268
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMII CTCAG 3 cut(s) 23, 224, 237
BseSI GKGCMC 2 cut(s) 379, 469
BseXI GCAGC 2 cut(s) 136, 205
BsiHKAI GWGCWC 2 cut(s) 379, 469
BsiHKCI CYCGRG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 379, 469
Bsp143I GATC 2 cut(s) 455, 492
BspACI CCGC 1 cut(s) 190
BspCNI CTCAG 3 cut(s) 24, 225, 238
BspLI GGNNCC 1 cut(s) 457
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 2 cut(s) 450, 463
BspQI GCTCTTC 1 cut(s) 357
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 2 cut(s) 455, 492
BssT1I CCWWGG 1 cut(s) 321
Bst6I CTCTTC 3 cut(s) 16, 74, 357
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 4 cut(s) 32, 65, 233, 246
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 184, 268
BstKTI GATC 2 cut(s) 458, 495
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 2 cut(s) 455, 492
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 460
BstSLI GKGCMC 2 cut(s) 379, 469
BstV1I GCAGC 2 cut(s) 136, 205
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BtsCI GGATG 2 cut(s) 184, 268
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 69
CviAII CATG 2 cut(s) 28, 217
CviJI RGCY 9 cut(s) 140, 149, 196, 206, 239, 367, 412, 480, 502
CviKI_1 RGCY 9 cut(s) 140, 149, 196, 206, 239, 367, 412, 480, 502
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 4 cut(s) 32, 65, 233, 246
DpnI GATC 2 cut(s) 457, 494
DpnII GATC 2 cut(s) 455, 492
Eam1104I CTCTTC 3 cut(s) 16, 74, 357
EarI CTCTTC 3 cut(s) 16, 74, 357
Eco130I CCWWGG 1 cut(s) 321
Eco32I GATATC 2 cut(s) 358, 424
Eco57I CTGAAG 2 cut(s) 398, 488
Eco88I CYCGRG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoRV GATATC 2 cut(s) 358, 424
EcoT14I CCWWGG 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 2 cut(s) 31, 220
FaiI YATR 9 cut(s) 29, 134, 154, 218, 311, 372, 438, 462, 511
FatI CATG 2 cut(s) 27, 216
Fnu4HI GCNGC 3 cut(s) 150, 191, 194
FokI GGATG 2 cut(s) 191, 275
Fsp4HI GCNGC 3 cut(s) 150, 191, 194
FspBI CTAG 2 cut(s) 197, 387
GluI GCNGC 3 cut(s) 150, 191, 194
Hin1II CATG 2 cut(s) 31, 220
HinfI GANTC 1 cut(s) 8
HphI GGTGA 3 cut(s) 317, 419, 509
Hpy166II GTNNAC 2 cut(s) 377, 467
Hpy188I TCNGA 2 cut(s) 20, 234
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 377, 467
HpyAV CCTTC 2 cut(s) 118, 244
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 152, 377, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 4 cut(s) 32, 65, 233, 246
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 2 cut(s) 31, 220
Kzo9I GATC 2 cut(s) 455, 492
LguI GCTCTTC 1 cut(s) 357
LpnPI CCDG 5 cut(s) 130, 225, 401, 438, 491
Lsp1109I GCAGC 2 cut(s) 136, 205
MaeI CTAG 2 cut(s) 197, 387
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 457, 494
MboI GATC 2 cut(s) 455, 492
MboII GAAGA 5 cut(s) 33, 91, 374, 412, 502
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 2 cut(s) 379, 469
MluCI AATT 2 cut(s) 101, 470
MnlI CCTC 8 cut(s) 27, 75, 169, 228, 253, 295, 319, 378
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 2 cut(s) 455, 492
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 31, 220
NlaIV GGNNCC 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 89
NspI RCATGY 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 3 cut(s) 151, 192, 195
PspN4I GGNNCC 1 cut(s) 457
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
RseI CAYNNNNRTG 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 3 cut(s) 150, 191, 194
Sau3AI GATC 2 cut(s) 455, 492
ScaI AGTACT 1 cut(s) 70
SduI GDGCHC 2 cut(s) 379, 469
SfcI CTRYAG 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 2 cut(s) 101, 470
SsiI CCGC 1 cut(s) 190
SspMI CTAG 2 cut(s) 197, 387
StyI CCWWGG 1 cut(s) 321
TaiI ACGT 1 cut(s) 122
TaqI TCGA 2 cut(s) 300, 360
TasI AATT 2 cut(s) 101, 470
TatI WGTACW 1 cut(s) 68
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 2 cut(s) 149, 193
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 1 cut(s) 207
VneI GTGCAC 2 cut(s) 375, 465
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 1 cut(s) 101
XceI RCATGY 1 cut(s) 31
XspI CTAG 2 cut(s) 197, 387
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.