Rh2BG274300

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007552
Physical Location & Seq
Forward (+)
29801768 .. 29816939
15172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG274300.1

Sequence Viewer

Length: 654 bp
ATGTTTCTAGCAGGACCTCCAGTACTTAGAACTTCTGGAATTTCCAAATTTGGGTACCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGTCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTGGAACTGAGAAGTTTAGCTTCCTCGGTAAAGATTTAATCAGATATAAAAATGCTGTTGAAGTTGATCTTCTTGTTTATGAGGCAATTGTAATATTTCAGGCTGGTGAGCAGATGTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTGAAAGTTGAAATGGTTTGTAGTCATCAGCGTGATATTGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATATCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATTTCCAAAGTCCCTCTTGAGAAAGTTTGCTTGGGCAATGGATGTCGGCCCTGACTTCAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

217

Amino Acids

24.6

Weight (kDa)

9.01

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 24 - 84 7.7e-18 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 54
AccI GTMKAC 1 cut(s) 206
AciI CCGC 1 cut(s) 190
AclWI GGATC 2 cut(s) 528, 541
AcsI RAATTY 3 cut(s) 39, 47, 101
AcuI CTGAAG 3 cut(s) 476, 566, 628
AfaI GTAC 3 cut(s) 24, 56, 70
AfiI CCNNNNNNNGG 2 cut(s) 51, 81
AgsI TTSAA 6 cut(s) 79, 124, 299, 358, 410, 440
AjuI GAANNNNNNNTTGG 2 cut(s) 601, 633
AluBI AGCT 6 cut(s) 140, 196, 258, 490, 558, 580
AluI AGCT 6 cut(s) 140, 196, 258, 490, 558, 580
Alw21I GWGCWC 2 cut(s) 457, 547
Alw44I GTGCAC 2 cut(s) 453, 543
AlwI GGATC 2 cut(s) 528, 541
AlwNI CAGNNNCTG 1 cut(s) 651
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 1 cut(s) 634
ApaLI GTGCAC 2 cut(s) 453, 543
ApeKI GCWGC 2 cut(s) 149, 193
ApoI RAATTY 3 cut(s) 39, 47, 101
Asp718I GGTACC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 2 cut(s) 14, 635
AsuHPI GGTGA 5 cut(s) 356, 389, 412, 497, 587
AsuNHI GCTAGC 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 2 cut(s) 457, 547
BamHI GGATCC 1 cut(s) 533
BanI GGYRCC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 457, 547
BbvI GCAGC 2 cut(s) 136, 205
BccI CCATC 1 cut(s) 173
BfaI CTAG 4 cut(s) 8, 59, 197, 465
BfmI CTRYAG 1 cut(s) 538
BisI GCNGC 3 cut(s) 150, 191, 194
BlsI GCNGC 3 cut(s) 151, 192, 195
BmcAI AGTACT 2 cut(s) 24, 70
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 14, 635
BmiI GGNNCC 2 cut(s) 56, 535
BmtI GCTAGC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 625
BsaJI CCNNGG 2 cut(s) 262, 393
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 81
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 630
BseDI CCNNGG 2 cut(s) 262, 393
BseGI GGATG 2 cut(s) 184, 634
BseLI CCNNNNNNNGG 2 cut(s) 51, 81
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 224, 237
BseNI ACTGG 1 cut(s) 20
BseSI GKGCMC 2 cut(s) 457, 547
BseXI GCAGC 2 cut(s) 136, 205
BshFI GGCC 1 cut(s) 636
BshNI GGYRCC 1 cut(s) 54
BsiHKAI GWGCWC 2 cut(s) 457, 547
BsiHKCI CYCGRG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 51, 81
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 1 cut(s) 636
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 457, 547
Bsp143I GATC 3 cut(s) 304, 533, 570
BspACI CCGC 1 cut(s) 190
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 2 cut(s) 225, 238
BspLI GGNNCC 2 cut(s) 56, 535
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 2 cut(s) 528, 541
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 262, 393
BssMI GATC 3 cut(s) 304, 533, 570
BssT1I CCWWGG 1 cut(s) 393
Bst6I CTCTTC 2 cut(s) 74, 435
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 4 cut(s) 26, 65, 233, 246
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 184, 634
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 3 cut(s) 307, 536, 573
BstMBI GATC 3 cut(s) 304, 533, 570
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstSFI CTRYAG 1 cut(s) 538
BstSLI GKGCMC 2 cut(s) 457, 547
BstV1I GCAGC 2 cut(s) 136, 205
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
BsuRI GGCC 1 cut(s) 636
BtsCI GGATG 2 cut(s) 184, 634
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 198
CaiI CAGNNNCTG 1 cut(s) 651
CfoI GCGC 1 cut(s) 522
Cfr13I GGNCC 2 cut(s) 14, 635
Csp6I GTAC 3 cut(s) 23, 55, 69
CviAII CATG 1 cut(s) 217
CviQI GTAC 3 cut(s) 23, 55, 69
DdeI CTNAG 4 cut(s) 26, 65, 233, 246
DpnI GATC 3 cut(s) 306, 535, 572
DpnII GATC 3 cut(s) 304, 533, 570
Eam1104I CTCTTC 2 cut(s) 74, 435
EarI CTCTTC 2 cut(s) 74, 435
Eco130I CCWWGG 1 cut(s) 393
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 3 cut(s) 476, 566, 628
Eco88I CYCGRG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 14
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 1 cut(s) 220
FaiI YATR 9 cut(s) 134, 154, 218, 285, 318, 383, 450, 516, 540
FalI AAGNNNNNCTT 6 cut(s) 242, 274, 291, 323, 587, 619
FaqI GGGAC 1 cut(s) 583
FatI CATG 1 cut(s) 216
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 3 cut(s) 150, 191, 194
FokI GGATG 2 cut(s) 191, 641
Fsp4HI GCNGC 3 cut(s) 150, 191, 194
FspBI CTAG 4 cut(s) 8, 59, 197, 465
GlaI GCGC 1 cut(s) 521
GluI GCNGC 3 cut(s) 150, 191, 194
HaeIII GGCC 1 cut(s) 636
HhaI GCGC 1 cut(s) 522
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HinfI GANTC 1 cut(s) 648
HphI GGTGA 5 cut(s) 356, 389, 412, 497, 587
Hpy166II GTNNAC 3 cut(s) 207, 455, 545
Hpy188I TCNGA 4 cut(s) 234, 281, 647, 653
Hpy188III TCNNGA 3 cut(s) 36, 161, 604
Hpy8I GTNNAC 3 cut(s) 207, 455, 545
HpyAV CCTTC 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 152, 455, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 4 cut(s) 26, 65, 233, 246
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 520
KpnI GGTACC 1 cut(s) 58
Kzo9I GATC 3 cut(s) 304, 533, 570
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 9 cut(s) 21, 33, 130, 225, 323, 327, 479, 516, 569
Lsp1109I GCAGC 2 cut(s) 136, 205
MaeI CTAG 4 cut(s) 8, 59, 197, 465
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 89
MalI GATC 3 cut(s) 306, 535, 572
MboI GATC 3 cut(s) 304, 533, 570
MboII GAAGA 5 cut(s) 91, 299, 452, 490, 580
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 2 cut(s) 457, 547
MluCI AATT 5 cut(s) 39, 47, 101, 324, 548
MseI TTAA 2 cut(s) 275, 560
MslI CAYNNNNRTG 1 cut(s) 429
MunI CAATTG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 3 cut(s) 304, 533, 570
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 2 cut(s) 56, 535
NmuCI GTSAC 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 648
PkrI GCNGC 3 cut(s) 151, 192, 195
PpuMI RGGWCCY 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 56, 535
PspPI GGNCC 2 cut(s) 14, 635
PspPPI RGGWCCY 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 651
PsuI RGATCY 1 cut(s) 533
RsaI GTAC 3 cut(s) 24, 56, 70
RsaNI GTAC 3 cut(s) 23, 55, 69
RseI CAYNNNNRTG 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 2 cut(s) 275, 560
SatI GCNGC 3 cut(s) 150, 191, 194
Sau3AI GATC 3 cut(s) 304, 533, 570
Sau96I GGNCC 2 cut(s) 14, 635
ScaI AGTACT 2 cut(s) 24, 70
SduI GDGCHC 2 cut(s) 457, 547
SfcI CTRYAG 1 cut(s) 538
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
Sse9I AATT 5 cut(s) 39, 47, 101, 324, 548
SsiI CCGC 1 cut(s) 190
SspI AATATT 1 cut(s) 333
SspMI CTAG 4 cut(s) 8, 59, 197, 465
StyI CCWWGG 1 cut(s) 393
TaiI ACGT 1 cut(s) 122
TaqI TCGA 1 cut(s) 372
TasI AATT 5 cut(s) 39, 47, 101, 324, 548
TatI WGTACW 2 cut(s) 22, 68
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 648
Tru1I TTAA 2 cut(s) 275, 560
Tru9I TTAA 2 cut(s) 275, 560
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 2 cut(s) 149, 193
Tsp45I GTSAC 1 cut(s) 89
TspRI CASTG 1 cut(s) 207
VneI GTGCAC 2 cut(s) 453, 543
VpaK11BI GGWCC 1 cut(s) 14
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 3 cut(s) 39, 47, 101
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 4 cut(s) 8, 59, 197, 465
ZrmI AGTACT 2 cut(s) 24, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.