Rh4CG237900

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007552
Physical Location & Seq
Forward (+)
48267585 .. 48270249
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG237900.1

Sequence Viewer

Length: 681 bp
ATGCTTTACAATGGGAGGCCAGTCAACTTGACTCCTGAACAAGAGGAGGACTTGGGTGGTTGTGATTTCACTCCGATATATGATTGGTATCGAAATGAATTGCAGAAGAAGAAACAGAGGAGTGACAAAGAGAAGAGAGCCGTCAAGCAAGAGGACTTGAAACAACAGCTGAAGTATAAGTGGGCTCTCGTTGATGGACCTCCAGTACTTAGAACTTCTGGAATTTCCAAATTTGGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGACAAATTTGAGAAAGCAAGGAAGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACATGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCAACCTATCTCATGGATAAACATGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTGTTAGAGGTAAGTGTAGCAGTGACGAACTGTTTGACAAGTTGGACACCCATAAATTGAATGCATATTTAAAGGCACTGATGTCCGGCCTTACAGCGGAAGTATTGCGTGCAGCCGTGCATACAATTAATGTGTCTATCACATTGGATAAAATAATAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

226

Amino Acids

25.82

Weight (kDa)

8.75

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 18 - 66 4.1e-08 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 85 - 158 1e-19 Eukaryotic DNA topoisomerase I, catalytic core
Topoisom_I PF01028 165 - 223 2.3e-09 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 373, 476, 614
AcsI RAATTY 3 cut(s) 222, 230, 284
AcuI CTGAAG 1 cut(s) 191
AfaI GTAC 2 cut(s) 207, 253
AfiI CCNNNNNNNGG 3 cut(s) 234, 264, 613
AgsI TTSAA 4 cut(s) 160, 262, 307, 577
AluBI AGCT 3 cut(s) 169, 323, 379
AluI AGCT 3 cut(s) 169, 323, 379
AoxI GGCC 2 cut(s) 17, 604
ApeKI GCWGC 3 cut(s) 332, 376, 629
ApoI RAATTY 3 cut(s) 222, 230, 284
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AseI ATTAAT 1 cut(s) 645
AspS9I GGNCC 1 cut(s) 197
AsuNHI GCTAGC 1 cut(s) 379
AvaII GGWCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 187
BbvI GCAGC 3 cut(s) 319, 388, 641
BccI CCATC 2 cut(s) 188, 356
BceAI ACGGC 2 cut(s) 125, 617
BfaI CTAG 2 cut(s) 242, 380
BisI GCNGC 5 cut(s) 333, 374, 377, 476, 630
BlsI GCNGC 5 cut(s) 334, 375, 378, 477, 631
BmcAI AGTACT 2 cut(s) 207, 253
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 197
BmtI GCTAGC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 264, 613
Bse1I ACTGG 2 cut(s) 20, 203
Bse8I GATNNNNATC 1 cut(s) 87
BseGI GGATG 2 cut(s) 367, 451
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 3 cut(s) 234, 264, 613
BseMII CTCAG 2 cut(s) 407, 420
BseNI ACTGG 2 cut(s) 20, 203
BseRI GAGGAG 2 cut(s) 59, 133
BseXI GCAGC 3 cut(s) 319, 388, 641
BsgI GTGCAG 1 cut(s) 648
BshFI GGCC 2 cut(s) 19, 606
BsiSI CCGG 1 cut(s) 603
BslI CCNNNNNNNGG 3 cut(s) 234, 264, 613
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 2 cut(s) 19, 606
Bsp1286I GDGCHC 1 cut(s) 187
BspACI CCGC 3 cut(s) 373, 476, 614
BspANI GGCC 2 cut(s) 19, 606
BspCNI CTCAG 2 cut(s) 408, 421
BspOI GCTAGC 1 cut(s) 383
BsrI ACTGG 2 cut(s) 20, 203
Bst4CI ACNGT 1 cut(s) 549
Bst6I CTCTTC 2 cut(s) 128, 257
BstC8I GCNNGC 2 cut(s) 381, 627
BstDEI CTNAG 4 cut(s) 209, 248, 416, 429
BstENI CCTNNNNNAGG 1 cut(s) 262
BstF5I GGATG 2 cut(s) 367, 451
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstV1I GCAGC 3 cut(s) 319, 388, 641
BsuRI GGCC 2 cut(s) 19, 606
BtsCI GGATG 2 cut(s) 367, 451
BtsI GCAGTG 2 cut(s) 390, 544
BtsIMutI CAGTG 3 cut(s) 390, 544, 593
Cac8I GCNNGC 2 cut(s) 381, 627
Cfr13I GGNCC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 206, 252
CviAII CATG 3 cut(s) 342, 400, 410
CviQI GTAC 2 cut(s) 206, 252
DdeI CTNAG 4 cut(s) 209, 248, 416, 429
DraI TTTAAA 1 cut(s) 588
Eam1104I CTCTTC 2 cut(s) 128, 257
EarI CTCTTC 2 cut(s) 128, 257
Eco24I GRGCYC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 187
FaeI CATG 3 cut(s) 345, 403, 413
FatI CATG 3 cut(s) 341, 399, 409
Fnu4HI GCNGC 5 cut(s) 333, 374, 377, 476, 630
FokI GGATG 2 cut(s) 374, 458
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 5 cut(s) 333, 374, 377, 476, 630
FspBI CTAG 2 cut(s) 242, 380
GluI GCNGC 5 cut(s) 333, 374, 377, 476, 630
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 19, 606
HapII CCGG 1 cut(s) 603
Hin1II CATG 3 cut(s) 345, 403, 413
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 603
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 75, 417
Hpy188III TCNNGA 2 cut(s) 35, 219
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 301, 427
HpyCH4III ACNGT 1 cut(s) 549
HpyCH4V TGCA 5 cut(s) 103, 335, 581, 629, 637
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 209, 248, 416, 429
Hsp92II CATG 3 cut(s) 345, 403, 413
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 7 cut(s) 33, 48, 204, 216, 313, 408, 616
Lsp1109I GCAGC 3 cut(s) 319, 388, 641
MaeI CTAG 2 cut(s) 242, 380
MaeIII GTNAC 3 cut(s) 122, 272, 539
MboII GAAGA 4 cut(s) 118, 121, 145, 274
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 7 cut(s) 98, 222, 230, 284, 572, 642, 675
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 540
Mph1103I ATGCAT 1 cut(s) 583
MseI TTAA 3 cut(s) 483, 587, 645
MslI CAYNNNNRTG 1 cut(s) 340
MspA1I CMGCKG 2 cut(s) 169, 614
MspI CCGG 1 cut(s) 603
Mva1269I GAATGC 1 cut(s) 583
MwoI GCNNNNNNNGC 1 cut(s) 329
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 3 cut(s) 345, 403, 413
NmuCI GTSAC 3 cut(s) 122, 272, 539
NsiI ATGCAT 1 cut(s) 583
PctI GAATGC 1 cut(s) 583
PkrI GCNGC 5 cut(s) 334, 375, 378, 477, 631
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PshBI ATTAAT 1 cut(s) 645
PspPI GGNCC 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 2 cut(s) 207, 253
RsaNI GTAC 2 cut(s) 206, 252
RseI CAYNNNNRTG 1 cut(s) 340
SaqAI TTAA 3 cut(s) 483, 587, 645
SatI GCNGC 5 cut(s) 333, 374, 377, 476, 630
Sau96I GGNCC 1 cut(s) 197
ScaI AGTACT 2 cut(s) 207, 253
SchI GAGTC 1 cut(s) 25
SduI GDGCHC 1 cut(s) 187
SetI ASST 7 cut(s) 171, 202, 325, 381, 395, 438, 529
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 340
Sse9I AATT 7 cut(s) 98, 222, 230, 284, 572, 642, 675
SsiI CCGC 3 cut(s) 373, 476, 614
SspMI CTAG 2 cut(s) 242, 380
TaaI ACNGT 1 cut(s) 549
TaqI TCGA 1 cut(s) 91
TasI AATT 7 cut(s) 98, 222, 230, 284, 572, 642, 675
TatI WGTACW 2 cut(s) 205, 251
TauI GCSGC 2 cut(s) 376, 478
Tru1I TTAA 3 cut(s) 483, 587, 645
Tru9I TTAA 3 cut(s) 483, 587, 645
TscAI CASTG 3 cut(s) 390, 544, 600
TseFI GTSAC 3 cut(s) 122, 272, 539
TseI GCWGC 3 cut(s) 332, 376, 629
Tsp45I GTSAC 3 cut(s) 122, 272, 539
TspDTI ATGAA 2 cut(s) 111, 468
TspRI CASTG 3 cut(s) 390, 544, 600
VpaK11BI GGWCC 1 cut(s) 197
VspI ATTAAT 1 cut(s) 645
XagI CCTNNNNNAGG 1 cut(s) 262
XapI RAATTY 3 cut(s) 222, 230, 284
XspI CTAG 2 cut(s) 242, 380
ZrmI AGTACT 2 cut(s) 207, 253
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.