Rmu_sc0001009.1_g000027

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001009.1
OG0007552
Physical Location & Seq
Reverse (-)
140579 .. 143716
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001009.1_g000027.1.cds

Sequence Viewer

Length: 618 bp
atgtcgtcgctagccaagccttctctcaccgcctcctccactcctaggtccgcgtaccccaacttccttgctaaccagcgcgcatttgcactcaccaccccattcctcgctgcaatgtcaaatccccgcaagctgttaccagagatgcagatccttgttgatgtggggtattctgtgagcaagcttttgaatctaccaacgcattatgcagctagtttgatattggttgcgtgttgcgcggtggttgttgaggatgatgatgaaactgatattgtcagttgccgcagattaaaagtagagaatgtaaaagcaatatcccctatcttgttagagtttagcttccttaaaaatgttgttgaagttgatcttcctgtttatgaggcaattgtaatatttcaggctggtaactatagcagtgacgaactatttgacaagttggacacccataaattgaatgcacatttaaaggcactgatgtctggccttacagcggaagtattgcatgcagccgttgaaatggtttgtaatcatcagcgtgatatcgaagagcctcatagtgcacaaatgtctaggataatggagaagattggtgagctcttcctgagttcagattggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

205

Amino Acids

22.68

Weight (kDa)

5.2

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 53, 81, 239
AciI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
AclWI GGATC 1 cut(s) 145
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 45, 490
AgsI TTSAA 4 cut(s) 190, 359, 454, 515
AluBI AGCT 5 cut(s) 133, 184, 212, 339, 595
AluI AGCT 5 cut(s) 133, 184, 212, 339, 595
Alw21I GWGCWC 2 cut(s) 562, 597
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 1 cut(s) 145
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 3 cut(s) 110, 209, 506
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspA2I CCTAGG 1 cut(s) 44
AspLEI GCGC 3 cut(s) 81, 83, 239
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 19, 85, 602
AsuNHI GCTAGC 1 cut(s) 10
AvaII GGWCC 1 cut(s) 48
AvrII CCTAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 597
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
Bbv12I GWGCWC 2 cut(s) 562, 597
BbvI GCAGC 3 cut(s) 97, 221, 518
BceAI ACGGC 1 cut(s) 494
BfaI CTAG 4 cut(s) 11, 45, 213, 570
BfmI CTRYAG 1 cut(s) 409
BisI GCNGC 4 cut(s) 111, 210, 283, 507
BlnI CCTAGG 1 cut(s) 44
BlsI GCNGC 4 cut(s) 112, 211, 284, 508
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmsI GCATC 1 cut(s) 135
BmtI GCTAGC 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 490
Bse3DI GCAATG 1 cut(s) 120
Bse8I GATNNNNATC 1 cut(s) 149
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 259
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 45, 490
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 593
BsePI GCGCGC 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 3 cut(s) 97, 221, 518
Bsh1236I CGCG 3 cut(s) 53, 81, 239
BshFI GGCC 1 cut(s) 483
BsiHKAI GWGCWC 2 cut(s) 562, 597
BslI CCNNNNNNNGG 2 cut(s) 45, 490
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 562, 597
Bsp143I GATC 2 cut(s) 150, 364
BspACI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 3 cut(s) 53, 81, 239
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 145
BspQI GCTCTTC 2 cut(s) 540, 602
BsrDI GCAATG 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 44
BssHII GCGCGC 1 cut(s) 79
BssMI GATC 2 cut(s) 150, 364
BssT1I CCWWGG 1 cut(s) 44
Bst6I CTCTTC 2 cut(s) 540, 602
BstC8I GCNNGC 5 cut(s) 12, 81, 131, 182, 504
BstDEI CTNAG 1 cut(s) 602
BstF5I GGATG 1 cut(s) 259
BstFNI CGCG 3 cut(s) 53, 81, 239
BstHHI GCGC 3 cut(s) 81, 83, 239
BstKTI GATC 2 cut(s) 153, 367
BstMBI GATC 2 cut(s) 150, 364
BstMWI GCNNNNNNNGC 2 cut(s) 16, 236
BstNSI RCATGY 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 53, 81, 239
BstV1I GCAGC 3 cut(s) 97, 221, 518
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 1 cut(s) 483
BtsCI GGATG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 421, 470
Cac8I GCNNGC 5 cut(s) 12, 81, 131, 182, 504
CfoI GCGC 3 cut(s) 81, 83, 239
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 55
CviAII CATG 1 cut(s) 503
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 602
DpnI GATC 2 cut(s) 152, 366
DpnII GATC 2 cut(s) 150, 364
DraI TTTAAA 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 540, 602
EarI CTCTTC 2 cut(s) 540, 602
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 595
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 1 cut(s) 506
FaiI YATR 6 cut(s) 207, 378, 411, 447, 504, 555
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 1 cut(s) 502
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 4 cut(s) 111, 210, 283, 507
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 4 cut(s) 111, 210, 283, 507
FspBI CTAG 4 cut(s) 11, 45, 213, 570
GlaI GCGC 3 cut(s) 80, 82, 238
GluI GCNGC 4 cut(s) 111, 210, 283, 507
HaeIII GGCC 1 cut(s) 483
HhaI GCGC 3 cut(s) 81, 83, 239
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 3 cut(s) 79, 81, 237
HinP1I GCGC 3 cut(s) 79, 81, 237
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 1 cut(s) 190
HphI GGTGA 3 cut(s) 19, 85, 602
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 1 cut(s) 610
Hpy188III TCNNGA 1 cut(s) 601
Hpy8I GTNNAC 1 cut(s) 560
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 30
HpyCH4V TGCA 8 cut(s) 89, 113, 148, 209, 458, 502, 506, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 236
HpyF3I CTNAG 1 cut(s) 602
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 3 cut(s) 79, 81, 237
Kzo9I GATC 2 cut(s) 150, 364
LguI GCTCTTC 2 cut(s) 540, 602
LpnPI CCDG 7 cut(s) 89, 153, 383, 384, 387, 465, 614
Lsp1109I GCAGC 3 cut(s) 97, 221, 518
LweI GCATC 1 cut(s) 135
MaeI CTAG 4 cut(s) 11, 45, 213, 570
MaeIII GTNAC 3 cut(s) 135, 404, 416
MalI GATC 2 cut(s) 152, 366
MboI GATC 2 cut(s) 150, 364
MboII GAAGA 4 cut(s) 359, 557, 589, 595
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 2 cut(s) 562, 597
MluCI AATT 2 cut(s) 384, 449
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 6 cut(s) 43, 46, 116, 244, 373, 561
MseI TTAA 3 cut(s) 290, 345, 464
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 491
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 460
MvnI CGCG 3 cut(s) 53, 81, 239
MwoI GCNNNNNNNGC 2 cut(s) 16, 236
NdeII GATC 2 cut(s) 150, 364
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 1 cut(s) 506
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 506
PaeI GCATGC 1 cut(s) 506
PauI GCGCGC 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 540, 602
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 4 cut(s) 112, 211, 284, 508
Psp124BI GAGCTC 1 cut(s) 597
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 150
PteI GCGCGC 1 cut(s) 79
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 534
SacI GAGCTC 1 cut(s) 597
SapI GCTCTTC 2 cut(s) 540, 602
SaqAI TTAA 3 cut(s) 290, 345, 464
SatI GCNGC 4 cut(s) 111, 210, 283, 507
Sau3AI GATC 2 cut(s) 150, 364
Sau96I GGNCC 1 cut(s) 48
SduI GDGCHC 2 cut(s) 562, 597
SetI ASST 6 cut(s) 50, 135, 186, 214, 341, 597
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 534
SphI GCATGC 1 cut(s) 506
Sse9I AATT 2 cut(s) 384, 449
SsiI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
SspI AATATT 1 cut(s) 393
SspMI CTAG 4 cut(s) 11, 45, 213, 570
SstI GAGCTC 1 cut(s) 597
StyI CCWWGG 1 cut(s) 44
TaqI TCGA 1 cut(s) 543
TasI AATT 2 cut(s) 384, 449
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 3 cut(s) 290, 345, 464
Tru9I TTAA 3 cut(s) 290, 345, 464
TscAI CASTG 2 cut(s) 421, 477
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 3 cut(s) 110, 209, 506
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 2 cut(s) 421, 477
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 48
XceI RCATGY 1 cut(s) 506
XmaJI CCTAGG 1 cut(s) 44
XspI CTAG 4 cut(s) 11, 45, 213, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.