Rh1AG127300

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007552
Physical Location & Seq
Forward (+)
23847058 .. 23850920
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG127300.1

Sequence Viewer

Length: 753 bp
ATGGGCAATATGTATTCTAGAAAAGTGGAATGGAAACTTTTTGTGAATGTAAATACTAGGTCCGCCTACCCCAACTTCCTTGCTAACCTGCGCGCATTTGCACTCACCCCATTCCTCGCTGCAGTGTCAAATCCCCGCAAGCTGTTACCAGAGATGCAGATCCTTGTCGATGTGGACCCAGGAATAGCTCCTTGCCGCCCATCTCAAATAGCAAACCATCGATGTGATGCTTTTATCAGGGTATTCTGTGAGCAAGCTTTTGAATCTGCCATCGCATTATGTAGCTGGTTTGATATTGGTTGCGTGCTGCACAGTGGTATGTCTTCATATGATATAGACGAACAACAAGTAACATTGTCACTTATATCGCACGGGATATGCAGCTCGGGAGTTTGCCAAAGTGGTTTATGCATGCTTGACTTTCCTTGCCTGCAGGTTGATGAGGATGATGATGAAACTGATATTGGCAGTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGTAATATCCCCTATCTTGTTAGAGATGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGATTGGTGAGCTTCAGGTTGATATCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATTTCCAAAGTCCCTCTTGAGAAAGTTTTCTTGGGCAATAGATGTCGGCCCTGACTTCAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

250

Amino Acids

28.0

Weight (kDa)

5.07

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 96, 424
AccII CGCG 1 cut(s) 93
AciI CCGC 4 cut(s) 63, 136, 196, 475
AclWI GGATC 3 cut(s) 154, 627, 640
AcuI CTGAAG 3 cut(s) 575, 665, 727
AgsI TTSAA 2 cut(s) 263, 575
AjnI CCWGG 1 cut(s) 178
AjuI GAANNNNNNNTTGG 4 cut(s) 447, 479, 700, 732
AluBI AGCT 8 cut(s) 142, 188, 257, 285, 384, 589, 657, 679
AluI AGCT 8 cut(s) 142, 188, 257, 285, 384, 589, 657, 679
Alw21I GWGCWC 1 cut(s) 646
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 3 cut(s) 154, 627, 640
AlwNI CAGNNNCTG 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 1 cut(s) 733
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 3 cut(s) 119, 307, 381
Asp700I GAANNNNTTC 1 cut(s) 712
AspLEI GCGC 3 cut(s) 93, 95, 621
AspS9I GGNCC 3 cut(s) 60, 175, 734
AsuHPI GGTGA 4 cut(s) 97, 560, 596, 686
AvaI CYCGRG 1 cut(s) 385
AvaII GGWCC 2 cut(s) 60, 175
BaeGI GKGCMC 1 cut(s) 646
BamHI GGATCC 1 cut(s) 632
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 3 cut(s) 106, 294, 393
BccI CCATC 3 cut(s) 208, 225, 278
BciT130I CCWGG 1 cut(s) 180
BfaI CTAG 2 cut(s) 18, 57
BfmI CTRYAG 3 cut(s) 120, 431, 637
BfuAI ACCTGC 2 cut(s) 96, 424
BisI GCNGC 5 cut(s) 120, 196, 308, 382, 475
BlsI GCNGC 5 cut(s) 121, 197, 309, 383, 476
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 2 cut(s) 60, 175
BmeT110I CYCGRG 1 cut(s) 385
BmgT120I GGNCC 3 cut(s) 60, 175, 734
BmiI GGNNCC 2 cut(s) 177, 634
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 2 cut(s) 144, 217
BpiI GAAGAC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 724
Bsa29I ATCGAT 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 178, 564
Bse8I GATNNNNATC 1 cut(s) 158
BseBI CCWGG 1 cut(s) 180
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 178, 564
BseGI GGATG 1 cut(s) 451
BseJI GATNNNNATC 1 cut(s) 158
BsePI GCGCGC 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 3 cut(s) 106, 294, 393
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 735
BshVI ATCGAT 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 385
BslFI GGGAC 1 cut(s) 682
BsmFI GGGAC 1 cut(s) 682
BsnI GGCC 1 cut(s) 735
BsoBI CYCGRG 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 3 cut(s) 159, 632, 669
BspACI CCGC 4 cut(s) 63, 136, 196, 475
BspANI GGCC 1 cut(s) 735
BspDI ATCGAT 1 cut(s) 220
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 177, 634
BspMAI CTGCAG 2 cut(s) 124, 435
BspMI ACCTGC 2 cut(s) 96, 424
BspPI GGATC 3 cut(s) 154, 627, 640
BssECI CCNNGG 2 cut(s) 178, 564
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 3 cut(s) 159, 632, 669
BssT1I CCWWGG 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 314
BstC8I GCNNGC 6 cut(s) 93, 140, 255, 305, 413, 431
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 3 cut(s) 93, 95, 621
BstKTI GATC 3 cut(s) 162, 635, 672
BstMBI GATC 3 cut(s) 159, 632, 669
BstMWI GCNNNNNNNGC 1 cut(s) 474
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 178
BstSFI CTRYAG 3 cut(s) 120, 431, 637
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 106, 294, 393
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 2 cut(s) 159, 632
BstYI RGATCY 2 cut(s) 159, 632
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 1 cut(s) 735
BsuTUI ATCGAT 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 319
BveI ACCTGC 2 cut(s) 96, 424
Cac8I GCNNGC 6 cut(s) 93, 140, 255, 305, 413, 431
CaiI CAGNNNCTG 1 cut(s) 750
CfoI GCGC 3 cut(s) 93, 95, 621
Cfr13I GGNCC 3 cut(s) 60, 175, 734
ClaI ATCGAT 1 cut(s) 220
CviAII CATG 1 cut(s) 412
CviJI RGCY 9 cut(s) 142, 188, 257, 285, 384, 589, 657, 679, 735
CviKI_1 RGCY 9 cut(s) 142, 188, 257, 285, 384, 589, 657, 679, 735
DpnI GATC 3 cut(s) 161, 634, 671
DpnII GATC 3 cut(s) 159, 632, 669
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 564
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 2 cut(s) 60, 175
Eco57I CTGAAG 3 cut(s) 575, 665, 727
Eco88I CYCGRG 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 178
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 564
EcoT22I ATGCAT 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 1 cut(s) 415
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 1 cut(s) 682
FatI CATG 1 cut(s) 411
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 328
Fnu4HI GCNGC 5 cut(s) 120, 196, 308, 382, 475
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 5 cut(s) 120, 196, 308, 382, 475
FspBI CTAG 2 cut(s) 18, 57
GlaI GCGC 3 cut(s) 92, 94, 620
GluI GCNGC 5 cut(s) 120, 196, 308, 382, 475
HaeIII GGCC 1 cut(s) 735
HhaI GCGC 3 cut(s) 93, 95, 621
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 3 cut(s) 91, 93, 619
HinP1I GCGC 3 cut(s) 91, 93, 619
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 2 cut(s) 263, 747
HphI GGTGA 4 cut(s) 97, 560, 596, 686
Hpy166II GTNNAC 2 cut(s) 175, 644
Hpy188I TCNGA 2 cut(s) 746, 752
Hpy188III TCNNGA 3 cut(s) 18, 387, 703
Hpy8I GTNNAC 2 cut(s) 175, 644
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4V TGCA 8 cut(s) 101, 122, 157, 310, 381, 411, 433, 644
HpyF10VI GCNNNNNNNGC 1 cut(s) 474
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 3 cut(s) 91, 93, 619
Kzo9I GATC 3 cut(s) 159, 632, 669
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 3 cut(s) 106, 294, 393
LweI GCATC 2 cut(s) 144, 217
MaeI CTAG 2 cut(s) 18, 57
MaeIII GTNAC 3 cut(s) 144, 349, 357
MalI GATC 3 cut(s) 161, 634, 671
MboI GATC 3 cut(s) 159, 632, 669
MboII GAAGA 2 cut(s) 315, 679
MflI RGATCY 2 cut(s) 159, 632
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 1 cut(s) 647
MnlI CCTC 5 cut(s) 125, 436, 538, 562, 710
Mph1103I ATGCAT 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 712
MseI TTAA 2 cut(s) 482, 659
MslI CAYNNNNRTG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 474
NdeI CATATG 1 cut(s) 328
NdeII GATC 3 cut(s) 159, 632, 669
NlaIII CATG 1 cut(s) 415
NlaIV GGNNCC 2 cut(s) 177, 634
NmuCI GTSAC 1 cut(s) 357
NsiI ATGCAT 1 cut(s) 413
NspI RCATGY 1 cut(s) 415
PaeI GCATGC 1 cut(s) 415
PauI GCGCGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 712
PfeI GAWTC 2 cut(s) 263, 747
PkrI GCNGC 5 cut(s) 121, 197, 309, 383, 476
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 177, 634
PspPI GGNCC 3 cut(s) 60, 175, 734
PstI CTGCAG 2 cut(s) 124, 435
PstNI CAGNNNCTG 1 cut(s) 750
PsuI RGATCY 2 cut(s) 159, 632
PteI GCGCGC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 482, 659
SatI GCNGC 5 cut(s) 120, 196, 308, 382, 475
Sau3AI GATC 3 cut(s) 159, 632, 669
Sau96I GGNCC 3 cut(s) 60, 175, 734
SbfI CCTGCAGG 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 180
SdaI CCTGCAGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 2 cut(s) 144, 217
SfcI CTRYAG 3 cut(s) 120, 431, 637
SinI GGWCC 2 cut(s) 60, 175
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SphI GCATGC 1 cut(s) 415
Sse8387I CCTGCAGG 1 cut(s) 435
Sse9I AATT 1 cut(s) 647
SsiI CCGC 4 cut(s) 63, 136, 196, 475
SspMI CTAG 2 cut(s) 18, 57
StyD4I CCNGG 1 cut(s) 178
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 1 cut(s) 314
TaqI TCGA 3 cut(s) 168, 220, 543
TasI AATT 1 cut(s) 647
TauI GCSGC 2 cut(s) 198, 477
TfiI GAWTC 2 cut(s) 263, 747
Tru1I TTAA 2 cut(s) 482, 659
Tru9I TTAA 2 cut(s) 482, 659
TscAI CASTG 2 cut(s) 129, 319
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 3 cut(s) 119, 307, 381
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 3 cut(s) 315, 468, 542
TspRI CASTG 2 cut(s) 129, 319
VneI GTGCAC 1 cut(s) 642
VpaK11BI GGWCC 2 cut(s) 60, 175
XbaI TCTAGA 1 cut(s) 17
XceI RCATGY 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 712
XspI CTAG 2 cut(s) 18, 57
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.