Rh2AG194800

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007552
Physical Location & Seq
Reverse (-)
19351448 .. 19359058
7611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG194800.1

Sequence Viewer

Length: 762 bp
ATGGCTGCTACCAGCAATATGGGTCGAGCTCCACTACTCCTCCTCCCTCCACTTGGTCTTCTTGGGCGTGTTGGTCTTTGGTGTCCTCACCGAGGAAGCACCGATTCCCTATTCGACCTTCCTCAGATGAGACTACTGTTTTTACTCAATTTGGCCATCGAGAAGGCCAAGTATGTCTTCCCCAAGCAGTTCGAGGCTCGGACTCTTGAGGAAGCTCTCATGTCAGGACCTCCAGTACTTAGAACTTCTGGAATTTCCAAATTTAGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCCTATCTCATGGATAAACTTGGTCAAAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGAGAATGTAAAAGCAATAGCCCCTATCTTGTTAGAGTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGGTTTGTAGTCATCAGCGTGATATCAAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATAGCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

253

Amino Acids

28.53

Weight (kDa)

9.62

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 95 - 150 4.2e-09 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 403, 492
AclWI GGATC 2 cut(s) 699, 712
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 3 cut(s) 252, 260, 314
AcuI CTGAAG 2 cut(s) 647, 737
AfaI GTAC 2 cut(s) 237, 283
AfiI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
AgsI TTSAA 4 cut(s) 292, 337, 535, 581
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AluBI AGCT 6 cut(s) 29, 215, 353, 661, 729, 751
AluI AGCT 6 cut(s) 29, 215, 353, 661, 729, 751
Alw21I GWGCWC 3 cut(s) 31, 628, 718
Alw26I GTCTC 1 cut(s) 124
Alw44I GTGCAC 2 cut(s) 624, 714
AlwI GGATC 2 cut(s) 699, 712
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 2 cut(s) 153, 165
ApaLI GTGCAC 2 cut(s) 624, 714
ApeKI GCWGC 3 cut(s) 5, 362, 406
ApoI RAATTY 3 cut(s) 252, 260, 314
AspLEI GCGC 1 cut(s) 693
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 4 cut(s) 80, 566, 668, 758
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 628, 718
BalI TGGCCA 1 cut(s) 155
BamHI GGATCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 50, 169
Bbv12I GWGCWC 3 cut(s) 31, 628, 718
BbvI GCAGC 2 cut(s) 349, 418
BccI CCATC 2 cut(s) 164, 386
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 2 cut(s) 272, 636
BfmI CTRYAG 1 cut(s) 709
BisI GCNGC 5 cut(s) 6, 363, 404, 407, 492
BlsI GCNGC 5 cut(s) 7, 364, 405, 408, 493
BmcAI AGTACT 2 cut(s) 237, 283
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 706
BmsI GCATC 1 cut(s) 684
BpiI GAAGAC 2 cut(s) 50, 169
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 91, 570
BsaXI ACNNNNNCTCC 4 cut(s) 24, 27, 54, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
Bse1I ACTGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 91, 570
BseGI GGATG 2 cut(s) 397, 468
BseLI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
BseMII CTCAG 2 cut(s) 137, 437
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 2 cut(s) 29, 32
BseSI GKGCMC 2 cut(s) 628, 718
BseXI GCAGC 2 cut(s) 349, 418
BshFI GGCC 2 cut(s) 155, 167
BsiHKAI GWGCWC 3 cut(s) 31, 628, 718
BsiHKCI CYCGRG 1 cut(s) 372
BslI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
BsmAI GTCTC 1 cut(s) 124
BsnI GGCC 2 cut(s) 155, 167
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 31, 628, 718
Bsp143I GATC 2 cut(s) 704, 741
BspACI CCGC 2 cut(s) 403, 492
BspANI GGCC 2 cut(s) 155, 167
BspCNI CTCAG 2 cut(s) 136, 438
BspLI GGNNCC 1 cut(s) 706
BspPI GGATC 2 cut(s) 699, 712
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 91, 570
BssMI GATC 2 cut(s) 704, 741
BssT1I CCWWGG 1 cut(s) 570
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 287
BstDEI CTNAG 4 cut(s) 123, 239, 278, 446
BstENI CCTNNNNNAGG 2 cut(s) 90, 292
BstF5I GGATG 2 cut(s) 397, 468
BstHHI GCGC 1 cut(s) 693
BstKTI GATC 2 cut(s) 707, 744
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 2 cut(s) 704, 741
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstSFI CTRYAG 1 cut(s) 709
BstSLI GKGCMC 2 cut(s) 628, 718
BstV1I GCAGC 2 cut(s) 349, 418
BstV2I GAAGAC 2 cut(s) 50, 169
BstX2I RGATCY 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 2 cut(s) 155, 167
BtsCI GGATG 2 cut(s) 397, 468
CfoI GCGC 1 cut(s) 693
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 236, 282
CviAII CATG 2 cut(s) 220, 417
CviQI GTAC 2 cut(s) 236, 282
DdeI CTNAG 4 cut(s) 123, 239, 278, 446
DpnI GATC 2 cut(s) 706, 743
DpnII GATC 2 cut(s) 704, 741
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 31
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 647, 737
Eco88I CYCGRG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 29
EcoNI CCTNNNNNAGG 2 cut(s) 90, 292
EcoO109I RGGNCCY 1 cut(s) 227
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 2 cut(s) 223, 420
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 2 cut(s) 219, 416
Fnu4HI GCNGC 5 cut(s) 6, 363, 404, 407, 492
FokI GGATG 2 cut(s) 404, 475
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 5 cut(s) 6, 363, 404, 407, 492
FspBI CTAG 2 cut(s) 272, 636
GlaI GCGC 1 cut(s) 692
GluI GCNGC 5 cut(s) 6, 363, 404, 407, 492
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 155, 167
HhaI GCGC 1 cut(s) 693
Hin1II CATG 2 cut(s) 223, 420
Hin6I GCGC 1 cut(s) 691
HinP1I GCGC 1 cut(s) 691
HinfI GANTC 2 cut(s) 104, 202
HphI GGTGA 4 cut(s) 80, 566, 668, 758
Hpy166II GTNNAC 2 cut(s) 626, 716
Hpy188I TCNGA 2 cut(s) 126, 201
Hpy188III TCNNGA 5 cut(s) 160, 206, 225, 249, 374
Hpy8I GTNNAC 2 cut(s) 626, 716
HpyAV CCTTC 4 cut(s) 128, 157, 331, 444
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 3 cut(s) 365, 626, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 4 cut(s) 123, 239, 278, 446
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 2 cut(s) 223, 420
HspAI GCGC 1 cut(s) 691
Kzo9I GATC 2 cut(s) 704, 741
LmnI GCTCC 2 cut(s) 34, 350
LpnPI CCDG 9 cut(s) 25, 210, 234, 246, 343, 425, 650, 687, 740
Lsp1109I GCAGC 2 cut(s) 349, 418
LweI GCATC 1 cut(s) 684
MaeI CTAG 2 cut(s) 272, 636
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 302
MalI GATC 2 cut(s) 706, 743
MboI GATC 2 cut(s) 704, 741
MboII GAAGA 5 cut(s) 50, 169, 304, 661, 751
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 3 cut(s) 31, 628, 718
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 5 cut(s) 148, 252, 260, 314, 719
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 196
Mox20I TGGCCA 1 cut(s) 155
MscI TGGCCA 1 cut(s) 155
MseI TTAA 1 cut(s) 731
MslI CAYNNNNRTG 1 cut(s) 600
Msp20I TGGCCA 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 2 cut(s) 704, 741
NlaIII CATG 2 cut(s) 223, 420
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 5 cut(s) 7, 364, 405, 408, 493
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 706
PspPI GGNCC 1 cut(s) 227
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 2 cut(s) 237, 283
RsaNI GTAC 2 cut(s) 236, 282
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 31
SaqAI TTAA 1 cut(s) 731
SatI GCNGC 5 cut(s) 6, 363, 404, 407, 492
Sau3AI GATC 2 cut(s) 704, 741
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 2 cut(s) 237, 283
SchI GAGTC 1 cut(s) 196
SduI GDGCHC 3 cut(s) 31, 628, 718
SfaNI GCATC 1 cut(s) 684
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 148, 252, 260, 314, 719
SsiI CCGC 2 cut(s) 403, 492
SspMI CTAG 2 cut(s) 272, 636
SstI GAGCTC 1 cut(s) 31
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 335
TaqI TCGA 5 cut(s) 25, 114, 159, 192, 549
TasI AATT 5 cut(s) 148, 252, 260, 314, 719
TatI WGTACW 2 cut(s) 235, 281
TauI GCSGC 2 cut(s) 406, 494
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 731
Tru9I TTAA 1 cut(s) 731
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 3 cut(s) 5, 362, 406
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 485
VneI GTGCAC 2 cut(s) 624, 714
VpaK11BI GGWCC 1 cut(s) 227
XagI CCTNNNNNAGG 2 cut(s) 90, 292
XapI RAATTY 3 cut(s) 252, 260, 314
XspI CTAG 2 cut(s) 272, 636
ZrmI AGTACT 2 cut(s) 237, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.