Rh2CG528700

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007552
Physical Location & Seq
Reverse (-)
70436336 .. 70438935
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG528700.1

Sequence Viewer

Length: 612 bp
ATGGTGGATTCTCTGTTCCGAAGAGACATGCTGAGGGGAGTGCTTCCCATTCTCTGTTCAGCAGGACCTCCAGTACTTAGAACTTCTAGAATTTCCAAATTTGGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGATTACATAGGAGCTATCAGGGCTGCATACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGTTGATGATGAAACTGATATTGTCAGCTTCCGCAGATTAAAAGTAGAGAATGTTGAAATGGTTTGTAGTCATCAGCGTGATATCGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATATCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATTTCCAAAGTCCCTCTTGAGAAAGTTTGCTTGGGCAATGGATGTCGGCCCTGACTTCATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

203

Amino Acids

23.15

Weight (kDa)

8.47

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 41 - 123 2.6e-21 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 241, 343
AclWI GGATC 2 cut(s) 486, 499
AcsI RAATTY 3 cut(s) 90, 98, 152
AcuI CTGAAG 2 cut(s) 434, 524
AfaI GTAC 2 cut(s) 75, 121
AfiI CCNNNNNNNGG 2 cut(s) 102, 132
AgsI TTSAA 3 cut(s) 130, 175, 368
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 6 cut(s) 191, 247, 339, 448, 516, 538
AluI AGCT 6 cut(s) 191, 247, 339, 448, 516, 538
Alw21I GWGCWC 2 cut(s) 415, 505
Alw26I GTCTC 1 cut(s) 18
Alw44I GTGCAC 2 cut(s) 411, 501
AlwI GGATC 2 cut(s) 486, 499
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 1 cut(s) 592
ApaLI GTGCAC 2 cut(s) 411, 501
ApeKI GCWGC 2 cut(s) 200, 244
ApoI RAATTY 3 cut(s) 90, 98, 152
AspLEI GCGC 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 65, 593
AsuHPI GGTGA 2 cut(s) 455, 545
AsuNHI GCTAGC 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 65
BaeGI GKGCMC 2 cut(s) 415, 505
BamHI GGATCC 1 cut(s) 491
Bbv12I GWGCWC 2 cut(s) 415, 505
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 187, 256
BccI CCATC 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 4 cut(s) 87, 110, 248, 423
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 3 cut(s) 201, 242, 245
BlsI GCNGC 3 cut(s) 202, 243, 246
BmcAI AGTACT 2 cut(s) 75, 121
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 65, 593
BmiI GGNNCC 1 cut(s) 493
BmtI GCTAGC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 54
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 583
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 132
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 588
BseGI GGATG 2 cut(s) 235, 592
BseLI CCNNNNNNNGG 2 cut(s) 102, 132
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 23, 275, 288
BseNI ACTGG 1 cut(s) 71
BseSI GKGCMC 2 cut(s) 415, 505
BseXI GCAGC 2 cut(s) 187, 256
BshFI GGCC 1 cut(s) 594
BsiHKAI GWGCWC 2 cut(s) 415, 505
BsiHKCI CYCGRG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 102, 132
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 1 cut(s) 594
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 415, 505
Bsp143I GATC 2 cut(s) 491, 528
BspACI CCGC 2 cut(s) 241, 343
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 3 cut(s) 24, 276, 289
BspLI GGNNCC 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 251
BspPI GGATC 2 cut(s) 486, 499
BspQI GCTCTTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 588
BsrI ACTGG 1 cut(s) 71
BssMI GATC 2 cut(s) 491, 528
Bst6I CTCTTC 3 cut(s) 16, 125, 393
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 5 cut(s) 32, 77, 116, 284, 297
BstENI CCTNNNNNAGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 235, 592
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 2 cut(s) 494, 531
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 2 cut(s) 491, 528
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 2 cut(s) 415, 505
BstV1I GCAGC 2 cut(s) 187, 256
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BsuRI GGCC 1 cut(s) 594
BtsCI GGATG 2 cut(s) 235, 592
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 249
CfoI GCGC 1 cut(s) 480
Cfr13I GGNCC 2 cut(s) 65, 593
Csp6I GTAC 2 cut(s) 74, 120
CviAII CATG 2 cut(s) 28, 268
CviQI GTAC 2 cut(s) 74, 120
DdeI CTNAG 5 cut(s) 32, 77, 116, 284, 297
DpnI GATC 2 cut(s) 493, 530
DpnII GATC 2 cut(s) 491, 528
Eam1104I CTCTTC 3 cut(s) 16, 125, 393
EarI CTCTTC 3 cut(s) 16, 125, 393
Eco32I GATATC 2 cut(s) 394, 460
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 434, 524
Eco88I CYCGRG 1 cut(s) 210
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 65
EcoRV GATATC 2 cut(s) 394, 460
FaeI CATG 2 cut(s) 31, 271
FaiI YATR 8 cut(s) 29, 185, 205, 269, 408, 474, 498, 605
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 1 cut(s) 541
FatI CATG 2 cut(s) 27, 267
Fnu4HI GCNGC 3 cut(s) 201, 242, 245
FokI GGATG 2 cut(s) 242, 599
Fsp4HI GCNGC 3 cut(s) 201, 242, 245
FspBI CTAG 4 cut(s) 87, 110, 248, 423
GlaI GCGC 1 cut(s) 479
GluI GCNGC 3 cut(s) 201, 242, 245
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 594
HhaI GCGC 1 cut(s) 480
Hin1II CATG 2 cut(s) 31, 271
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HinfI GANTC 1 cut(s) 8
HphI GGTGA 2 cut(s) 455, 545
Hpy166II GTNNAC 2 cut(s) 413, 503
Hpy188I TCNGA 3 cut(s) 20, 285, 611
Hpy188III TCNNGA 3 cut(s) 87, 212, 562
Hpy8I GTNNAC 2 cut(s) 413, 503
HpyAV CCTTC 2 cut(s) 169, 295
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 3 cut(s) 203, 413, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 5 cut(s) 32, 77, 116, 284, 297
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 2 cut(s) 31, 271
HspAI GCGC 1 cut(s) 478
Kzo9I GATC 2 cut(s) 491, 528
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 7 cut(s) 48, 84, 181, 276, 437, 474, 527
Lsp1109I GCAGC 2 cut(s) 187, 256
MaeI CTAG 4 cut(s) 87, 110, 248, 423
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 493, 530
MboI GATC 2 cut(s) 491, 528
MboII GAAGA 5 cut(s) 33, 142, 410, 448, 538
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 2 cut(s) 415, 505
MluCI AATT 4 cut(s) 90, 98, 152, 506
MnlI CCTC 8 cut(s) 27, 78, 126, 220, 279, 304, 414, 569
MseI TTAA 2 cut(s) 350, 518
MslI CAYNNNNRTG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeII GATC 2 cut(s) 491, 528
NheI GCTAGC 1 cut(s) 247
NlaIII CATG 2 cut(s) 31, 271
NlaIV GGNNCC 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 140
NspI RCATGY 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 3 cut(s) 202, 243, 246
PpuMI RGGWCCY 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 65
PspN4I GGNNCC 1 cut(s) 493
PspPI GGNCC 2 cut(s) 65, 593
PspPPI RGGWCCY 1 cut(s) 65
PsuI RGATCY 1 cut(s) 491
RsaI GTAC 2 cut(s) 75, 121
RsaNI GTAC 2 cut(s) 74, 120
RseI CAYNNNNRTG 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 2 cut(s) 350, 518
SatI GCNGC 3 cut(s) 201, 242, 245
Sau3AI GATC 2 cut(s) 491, 528
Sau96I GGNCC 2 cut(s) 65, 593
ScaI AGTACT 2 cut(s) 75, 121
SduI GDGCHC 2 cut(s) 415, 505
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 4 cut(s) 90, 98, 152, 506
SsiI CCGC 2 cut(s) 241, 343
SspMI CTAG 4 cut(s) 87, 110, 248, 423
TaiI ACGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 396
TasI AATT 4 cut(s) 90, 98, 152, 506
TatI WGTACW 2 cut(s) 73, 119
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 350, 518
Tru9I TTAA 2 cut(s) 350, 518
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 2 cut(s) 200, 244
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 336, 592
TspRI CASTG 1 cut(s) 258
VneI GTGCAC 2 cut(s) 411, 501
VpaK11BI GGWCC 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 130
XapI RAATTY 3 cut(s) 90, 98, 152
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 1 cut(s) 31
XspI CTAG 4 cut(s) 87, 110, 248, 423
ZrmI AGTACT 2 cut(s) 75, 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.