RLG00000008418

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005896
Physical Location & Seq
Forward (+)
30285382 .. 30287997
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008418

Sequence Viewer

Length: 543 bp
ATGGCGGTCCGGCGGCTGTCTGGAGATACCGGCCGGGTAGAACACTTGGCGATCCAGCGGCGGTGGCCGTGGTGGGGATGGGCCAAGCTTGGCTGGCGGCTTATGGTCTTCCTTCGGAGACCATACTTCGCCGTAAAGCTCGCCTCCCCTTTAACCCGACTCGACTGCGACGTCTTGCTATTGGGCTTCTGCATTGGGCTCCAACTTCTGGTGAACGACCTTTCTCCCAAAAAGAAGACCGGTGAAAGAATGTGTATTGTTCGATGTGAAAGGCATTATGGAAGTCTTGTTGCCTCTGGTTCTGTTGATTTTTGGGTCATGGAAAGGTATGGAGACAATGACTCATGGAACATGCTTTATAGCTTTAGGATCTCCAACGAGTGTCCGATTAAGTTTCTGAAGCCACTTGTAGTTACGGAGAGTAGTCAAGTTCTAGTGAGATGTTCTACTGCTGCAGAGTTGGAGTTGGTAAGGATTCGTGGACAAGAAGAGGAGGAGCGTGGTACCTATATGAATGAGGTCGAACAAATGTTATGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

181

Amino Acids

21.01

Weight (kDa)

8.79

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 208
AciI CCGC 5 cut(s) 5, 13, 58, 61, 97
AclWI GGATC 2 cut(s) 46, 377
AcoI YGGCCR 2 cut(s) 31, 65
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 2 cut(s) 74, 208
AgeI ACCGGT 1 cut(s) 239
AluBI AGCT 3 cut(s) 88, 139, 363
AluI AGCT 3 cut(s) 88, 139, 363
Alw26I GTCTC 2 cut(s) 112, 327
AlwI GGATC 2 cut(s) 46, 377
AoxI GGCC 3 cut(s) 31, 65, 81
ApeKI GCWGC 1 cut(s) 452
AsiGI ACCGGT 1 cut(s) 239
Asp718I GGTACC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 7, 81
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 223, 254
AvaII GGWCC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 503
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 2 cut(s) 100, 242
BbvI GCAGC 1 cut(s) 439
BccI CCATC 1 cut(s) 72
BceAI ACGGC 2 cut(s) 52, 116
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 112, 327
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 4 cut(s) 14, 59, 98, 453
BlsI GCNGC 4 cut(s) 15, 60, 99, 454
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 81
BmiI GGNNCC 2 cut(s) 200, 505
BmrFI CCNGG 1 cut(s) 35
BpiI GAAGAC 2 cut(s) 100, 242
BpmI CTGGAG 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 239
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 208
Bse118I RCCGGY 2 cut(s) 29, 239
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 2 cut(s) 74, 208
BseRI GAGGAG 2 cut(s) 506, 509
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 3 cut(s) 33, 67, 83
BshNI GGYRCC 1 cut(s) 503
BshTI ACCGGT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 34
BsiSI CCGG 4 cut(s) 10, 30, 34, 240
BslI CCNNNNNNNGG 2 cut(s) 74, 208
BsmAI GTCTC 2 cut(s) 112, 327
BsnI GGCC 3 cut(s) 33, 67, 83
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 51, 369
BspACI CCGC 5 cut(s) 5, 13, 58, 61, 97
BspANI GGCC 3 cut(s) 33, 67, 83
BspLI GGNNCC 2 cut(s) 200, 505
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 2 cut(s) 46, 377
BspT107I GGYRCC 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 112
BsrFI RCCGGY 2 cut(s) 29, 239
BssAI RCCGGY 2 cut(s) 29, 239
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 2 cut(s) 51, 369
BssNI GRCGYC 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 95, 141
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 1 cut(s) 83
BstKTI GATC 2 cut(s) 54, 372
BstMAI GTCTC 2 cut(s) 112, 327
BstMBI GATC 2 cut(s) 51, 369
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 64, 94
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 2 cut(s) 100, 242
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 3 cut(s) 33, 67, 83
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 95, 141
Cfr10I RCCGGY 2 cut(s) 29, 239
Cfr13I GGNCC 2 cut(s) 7, 81
CpoI CGGWCCG 1 cut(s) 7
Csp6I GTAC 1 cut(s) 504
CspAI ACCGGT 1 cut(s) 239
CspI CGGWCCG 1 cut(s) 7
CviAII CATG 4 cut(s) 319, 345, 352, 540
CviQI GTAC 1 cut(s) 504
DpnI GATC 2 cut(s) 53, 371
DpnII GATC 2 cut(s) 51, 369
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 2 cut(s) 31, 65
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EclXI CGGCCG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 7
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 4 cut(s) 322, 348, 355, 543
FatI CATG 4 cut(s) 318, 344, 351, 539
Fnu4HI GCNGC 4 cut(s) 14, 59, 98, 453
FokI GGATG 1 cut(s) 90
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 4 cut(s) 14, 59, 98, 453
FspBI CTAG 1 cut(s) 434
GluI GCNGC 4 cut(s) 14, 59, 98, 453
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 3 cut(s) 33, 67, 83
HapII CCGG 4 cut(s) 10, 30, 34, 240
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 4 cut(s) 322, 348, 355, 543
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 159, 341, 475
HpaII CCGG 4 cut(s) 10, 30, 34, 240
HphI GGTGA 2 cut(s) 223, 254
Hpy166II GTNNAC 2 cut(s) 214, 482
Hpy188I TCNGA 3 cut(s) 117, 387, 399
Hpy188III TCNNGA 1 cut(s) 21
Hpy8I GTNNAC 2 cut(s) 214, 482
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 192, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 94
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 4 cut(s) 322, 348, 355, 543
KpnI GGTACC 1 cut(s) 507
Kzo9I GATC 2 cut(s) 51, 369
LmnI GCTCC 2 cut(s) 204, 496
LpnPI CCDG 9 cut(s) 6, 23, 43, 47, 68, 79, 194, 253, 282
Lsp1109I GCAGC 1 cut(s) 439
MaeI CTAG 1 cut(s) 434
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 412
MalI GATC 2 cut(s) 53, 371
MboI GATC 2 cut(s) 51, 369
MboII GAAGA 3 cut(s) 100, 247, 500
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 201
MlyI GAGTC 2 cut(s) 153, 335
MmeI TCCRAC 3 cut(s) 226, 399, 441
MnlI CCTC 5 cut(s) 154, 304, 484, 487, 511
MseI TTAA 2 cut(s) 152, 390
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 4 cut(s) 10, 30, 34, 240
MspR9I CCNGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 64, 94
NciI CCSGG 1 cut(s) 35
NdeII GATC 2 cut(s) 51, 369
NlaIII CATG 4 cut(s) 322, 348, 355, 543
NlaIV GGNNCC 2 cut(s) 200, 505
NspI RCATGY 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 1 cut(s) 475
PflMI CCANNNNNTGG 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 4 cut(s) 15, 60, 99, 454
PleI GAGTC 2 cut(s) 153, 335
PpsI GAGTC 2 cut(s) 153, 335
PspN4I GGNNCC 2 cut(s) 200, 505
PspPI GGNCC 2 cut(s) 7, 81
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 369
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
Rsr2I CGGWCCG 1 cut(s) 7
RsrII CGGWCCG 1 cut(s) 7
SaqAI TTAA 2 cut(s) 152, 390
SatI GCNGC 4 cut(s) 14, 59, 98, 453
Sau3AI GATC 2 cut(s) 51, 369
Sau96I GGNCC 2 cut(s) 7, 81
SchI GAGTC 2 cut(s) 153, 335
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 201
SetI ASST 8 cut(s) 90, 141, 174, 222, 329, 365, 509, 522
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 1 cut(s) 7
SsiI CCGC 5 cut(s) 5, 13, 58, 61, 97
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 33
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 162, 262, 522
TauI GCSGC 3 cut(s) 16, 61, 100
TfiI GAWTC 1 cut(s) 475
Tru1I TTAA 2 cut(s) 152, 390
Tru9I TTAA 2 cut(s) 152, 390
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 7
XceI RCATGY 1 cut(s) 355
XspI CTAG 1 cut(s) 434
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.