RLG00000016544

Multiple C2 and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007532
Physical Location & Seq
Reverse (-)
7508557 .. 7508967
411 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016544

Sequence Viewer

Length: 411 bp
ATGGTGGCCACATGGCTCGCACGCTTCGAGCCGCTGCTAGGGCTGGAGGTGGTGCAGTACATGTTAGATGCTGACTCGCACATGTGGAGCATGAGGAAGAGCAAAGCGAATTGGGTTTGGATCGTAGCGATGCTGGCGTGGTTGGTCGGGTTAGCGAAGTGGTTGGATGATATACAGAGGTGGAGGAACTCGGTGACGACGGTTTTGGTGCACATGTTGTATTTGGTTCTGGTTTGGTACCCAGATTTGATAATCCCGACTGGGTTTCTATATGTGTTTATGATTGGGGTTTGGTACTACCGGTTCAAGCCCAAGATACCGGCTCGAATGGACCTCTGGCTCTCCCAGGCGGATACGGTCGACCAGGATGAACTTGATGAGGAGTTCGATACTTTCTCAGAGCTGCCGTAG

Protein Analysis

137

Amino Acids

16.22

Weight (kDa)

4.72

Isoelectric Point (pI)

24.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRT_C PF08372 86 - 132 1e-14 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 360
AciI CCGC 2 cut(s) 32, 350
AclWI GGATC 1 cut(s) 128
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 3 cut(s) 59, 239, 296
AfiI CCNNNNNNNGG 1 cut(s) 38
AflIII ACRYGT 3 cut(s) 60, 81, 213
AgeI ACCGGT 1 cut(s) 300
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 345, 363
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 213
Alw44I GTGCAC 1 cut(s) 209
AlwI GGATC 1 cut(s) 128
AoxI GGCC 1 cut(s) 6
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 34, 403
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
AsiGI ACCGGT 1 cut(s) 300
Asp718I GGTACC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 8
BanI GGYRCC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 21, 390
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 2 cut(s) 347, 365
BciVI GTATCC 1 cut(s) 346
BfaI CTAG 1 cut(s) 38
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 3 cut(s) 32, 35, 404
BlsI GCNGC 3 cut(s) 33, 36, 405
Bme1390I CCNGG 2 cut(s) 347, 365
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 2 cut(s) 347, 365
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 58, 120
BmuI ACTGGG 1 cut(s) 270
BpmI CTGGAG 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 2 cut(s) 300, 319
Bse1I ACTGG 1 cut(s) 265
BseBI CCWGG 2 cut(s) 347, 365
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 2 cut(s) 172, 373
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 2 cut(s) 21, 390
BsgI GTGCAG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 237
BshTI ACCGGT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 2 cut(s) 301, 320
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 2 cut(s) 32, 350
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 239
BspPI GGATC 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 237
BsrFI RCCGGY 2 cut(s) 300, 319
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 2 cut(s) 300, 319
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 1 cut(s) 120
Bst2UI CCWGG 2 cut(s) 347, 365
Bst4CI ACNGT 2 cut(s) 202, 358
Bst6I CTCTTC 1 cut(s) 92
BstC8I GCNNGC 3 cut(s) 18, 22, 135
BstDEI CTNAG 1 cut(s) 397
BstF5I GGATG 2 cut(s) 172, 373
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 2 cut(s) 40, 134
BstNI CCWGG 2 cut(s) 347, 365
BstNSI RCATGY 3 cut(s) 64, 85, 217
BstSCI CCNGG 2 cut(s) 345, 363
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 21, 390
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 8
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 172, 373
Cac8I GCNNGC 3 cut(s) 18, 22, 135
Cfr10I RCCGGY 2 cut(s) 300, 319
Cfr13I GGNCC 1 cut(s) 331
Csp6I GTAC 3 cut(s) 58, 238, 295
CspAI ACCGGT 1 cut(s) 300
CviAII CATG 5 cut(s) 12, 61, 82, 91, 214
CviJI RGCY 8 cut(s) 8, 16, 31, 43, 310, 323, 340, 403
CviKI_1 RGCY 8 cut(s) 8, 16, 31, 43, 310, 323, 340, 403
CviQI GTAC 3 cut(s) 58, 238, 295
DdeI CTNAG 1 cut(s) 397
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 345, 363
FaeI CATG 5 cut(s) 15, 64, 85, 94, 217
FaiI YATR 9 cut(s) 13, 62, 83, 92, 173, 215, 271, 273, 281
FatI CATG 5 cut(s) 11, 60, 81, 90, 213
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 32, 35, 404
FokI GGATG 2 cut(s) 179, 380
Fsp4HI GCNGC 3 cut(s) 32, 35, 404
FspBI CTAG 1 cut(s) 38
GluI GCNGC 3 cut(s) 32, 35, 404
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 301, 320
Hin1II CATG 5 cut(s) 15, 64, 85, 94, 217
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 74
HpaII CCGG 2 cut(s) 301, 320
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 211, 361
Hpy188I TCNGA 1 cut(s) 400
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 2 cut(s) 211, 361
Hpy99I CGWCG 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 202, 358
HpyCH4V TGCA 2 cut(s) 55, 211
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 134
HpyF3I CTNAG 1 cut(s) 397
Hsp92II CATG 5 cut(s) 15, 64, 85, 94, 217
KpnI GGTACC 1 cut(s) 241
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 2 cut(s) 21, 390
LweI GCATC 2 cut(s) 58, 120
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 1 cut(s) 109
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 40, 87, 171, 177, 344, 373
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 2 cut(s) 301, 320
MspR9I CCNGG 2 cut(s) 347, 365
MvaI CCWGG 2 cut(s) 347, 365
MwoI GCNNNNNNNGC 2 cut(s) 40, 134
NdeII GATC 1 cut(s) 120
NlaIII CATG 5 cut(s) 15, 64, 85, 94, 217
NlaIV GGNNCC 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 3 cut(s) 64, 85, 217
PciI ACATGT 3 cut(s) 60, 81, 213
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PinAI ACCGGT 1 cut(s) 300
PkrI GCNGC 3 cut(s) 33, 36, 405
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PscI ACATGT 3 cut(s) 60, 81, 213
Psp6I CCWGG 2 cut(s) 345, 363
PspGI CCWGG 2 cut(s) 345, 363
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 1 cut(s) 331
PsrI GAACNNNNNNTAC 2 cut(s) 287, 319
RsaI GTAC 3 cut(s) 59, 239, 296
RsaNI GTAC 3 cut(s) 58, 238, 295
SalI GTCGAC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 92
SatI GCNGC 3 cut(s) 32, 35, 404
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 347, 365
SduI GDGCHC 1 cut(s) 213
SetI ASST 4 cut(s) 51, 182, 336, 405
SfaNI GCATC 2 cut(s) 58, 120
SinI GGWCC 1 cut(s) 331
Sse9I AATT 1 cut(s) 109
SsiI CCGC 2 cut(s) 32, 350
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 345, 363
TaaI ACNGT 2 cut(s) 202, 358
TaqI TCGA 4 cut(s) 27, 325, 360, 387
TasI AATT 1 cut(s) 109
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 34
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 34, 403
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 384
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 331
XceI RCATGY 3 cut(s) 64, 85, 217
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.