Rroxscaffold_3G00257030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007532
Physical Location & Seq
Forward (+)
51456343 .. 51462896
6554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257030.1

Sequence Viewer

Length: 408 bp
ATGGGTGCTACCGTGCTGGTGGAGGAAAAAGAACACAACCACATCAAATCCGCGTTTCAGGTTCATTTACTGCTGATGATGTACGAGCAGTACGAGGGTTTTGAACAGAAGAAGAAAGAGAGAGACAGGGACGTCATGGACAAATTATCACCATCGAGAGGGACCGGATCATCGTCGGGTCTAGGCAAGGACATGTCGCCGTTAGGGGGAGAGGGGACGAGATTAATGGATAGATTGGTTGGTAGTTGGCGGCGATTTGGCGTGTGGCTTGAGTTCGGTGTTGACCATGGTGGATTTGTGGCTGGTTATGGGTGGAGTGCACTATGGATAAGGACCCCGGCGGGCAGAGCAGCGGCGATGCTGTTTAGTGTTGCCGGCCAATGGGTCAATGCTAGGTTGGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.02

Weight (kDa)

9.34

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AatII GACGTC 1 cut(s) 135
AccII CGCG 1 cut(s) 53
AciI CCGC 4 cut(s) 51, 250, 341, 353
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 1 cut(s) 376
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 2 cut(s) 83, 92
AfiI CCNNNNNNNGG 3 cut(s) 158, 206, 381
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 1 cut(s) 104
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 1 cut(s) 175
AoxI GGCC 1 cut(s) 376
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 1 cut(s) 350
AseI ATTAAT 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 162, 333
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 141
AvaII GGWCC 2 cut(s) 162, 333
BaeGI GKGCMC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 182, 393
BisI GCNGC 3 cut(s) 251, 351, 354
BlsI GCNGC 3 cut(s) 252, 352, 355
Bme1390I CCNGG 1 cut(s) 338
Bme18I GGWCC 2 cut(s) 162, 333
BmgT120I GGNCC 2 cut(s) 162, 333
BmiI GGNNCC 2 cut(s) 163, 335
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 286, 336
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 206, 381
Bse118I RCCGGY 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 286, 336
BseLI CCNNNNNNNGG 3 cut(s) 158, 206, 381
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 3 cut(s) 165, 338, 375
BslFI GGGAC 3 cut(s) 143, 175, 229
BslI CCNNNNNNNGG 3 cut(s) 158, 206, 381
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 3 cut(s) 143, 175, 229
BsnI GGCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 4 cut(s) 51, 250, 341, 353
BspANI GGCC 1 cut(s) 378
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 163, 335
BspPI GGATC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 374
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 286, 336
BssMI GATC 1 cut(s) 167
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 13
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 343, 376
BstDSI CCRYGG 1 cut(s) 286
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 1 cut(s) 170
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 167
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 362
BsuRI GGCC 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 343, 376
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 162, 333
Csp6I GTAC 2 cut(s) 82, 91
CviAII CATG 3 cut(s) 136, 193, 287
CviJI RGCY 3 cut(s) 268, 302, 378
CviKI_1 RGCY 3 cut(s) 268, 302, 378
CviQI GTAC 2 cut(s) 82, 91
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 286
Eco47I GGWCC 2 cut(s) 162, 333
EcoO109I RGGNCCY 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 3 cut(s) 139, 196, 290
FaiI YATR 5 cut(s) 137, 194, 288, 309, 325
FaqI GGGAC 3 cut(s) 143, 175, 229
FatI CATG 3 cut(s) 135, 192, 286
FauI CCCGC 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 251, 351, 354
Fsp4HI GCNGC 3 cut(s) 251, 351, 354
FspBI CTAG 2 cut(s) 182, 393
GluI GCNGC 3 cut(s) 251, 351, 354
HaeIII GGCC 1 cut(s) 378
HapII CCGG 3 cut(s) 165, 338, 375
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 3 cut(s) 139, 196, 290
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HpaII CCGG 3 cut(s) 165, 338, 375
HphI GGTGA 1 cut(s) 141
Hpy166II GTNNAC 2 cut(s) 283, 320
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 2 cut(s) 283, 320
Hpy99I CGWCG 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 3 cut(s) 139, 196, 290
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Kzo9I GATC 1 cut(s) 167
LpnPI CCDG 7 cut(s) 2, 44, 112, 178, 288, 351, 388
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 348
MaeI CTAG 2 cut(s) 182, 393
MaeII ACGT 1 cut(s) 132
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 2 cut(s) 121, 124
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 1 cut(s) 143
MnlI CCTC 4 cut(s) 16, 88, 152, 205
MroNI GCCGGC 1 cut(s) 374
MseI TTAA 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 3 cut(s) 165, 338, 375
MspR9I CCNGG 1 cut(s) 338
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 347
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 1 cut(s) 286
NdeII GATC 1 cut(s) 167
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 3 cut(s) 139, 196, 290
NlaIV GGNNCC 2 cut(s) 163, 335
NspI RCATGY 1 cut(s) 196
PciI ACATGT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdiI GCCGGC 1 cut(s) 376
PkrI GCNGC 3 cut(s) 252, 352, 355
PpuMI RGGWCCY 1 cut(s) 333
PscI ACATGT 1 cut(s) 192
PshBI ATTAAT 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 163, 335
PspPI GGNCC 2 cut(s) 162, 333
PspPPI RGGWCCY 1 cut(s) 333
RsaI GTAC 2 cut(s) 83, 92
RsaNI GTAC 2 cut(s) 82, 91
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 3 cut(s) 251, 351, 354
Sau3AI GATC 1 cut(s) 167
Sau96I GGNCC 2 cut(s) 162, 333
ScrFI CCNGG 1 cut(s) 338
SduI GDGCHC 1 cut(s) 322
SetI ASST 3 cut(s) 63, 135, 398
SfaNI GCATC 1 cut(s) 348
SinI GGWCC 2 cut(s) 162, 333
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 1 cut(s) 143
SsiI CCGC 4 cut(s) 51, 250, 341, 353
SspMI CTAG 2 cut(s) 182, 393
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 155
TasI AATT 1 cut(s) 143
TauI GCSGC 2 cut(s) 253, 356
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 53
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 2 cut(s) 162, 333
VspI ATTAAT 1 cut(s) 224
XceI RCATGY 1 cut(s) 196
XspI CTAG 2 cut(s) 182, 393
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.