Rh2DG654600

Multiple C2 and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007532
Physical Location & Seq
Reverse (-)
87966665 .. 87967075
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG654600.1

Sequence Viewer

Length: 411 bp
ATGGTGGCCGCATGGCTAGTACGCTCCGAGCCGCCACTAGGGCCGGAGGTGGTGCAGTACATGTTGGATGCTGACTCACACACGTGGAGCATGAGGAAGAGCAAAGCGAATTGGTTTCAGATCGTGGCGATGCTGGCATGGTTGGTCGGGTTAGCAAAGTGGTTGGATGATATACGGATGTGGAGGAACTCGGTCACGACTATTTTGGTGCACATGTTGCATTTGGTTCTGGTTTGGTACCCGGATTTCATAATCCCGACTGGGATTCTGTATGTGTTTCTGATTGGGGTTTGGTACTACCGATTCAGGCCCAAGATACCGGCTGGAATGGACCTCTGGCTCTCCCAGGCGGATACAATTGACTCGGATAAACTTGATGAGGAGTTCGACACTTTCTCGAAGCTGCTGTAG

Protein Analysis

136

Amino Acids

15.98

Weight (kDa)

5.48

Isoelectric Point (pI)

28.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRT_C PF08372 86 - 132 2.8e-13 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AciI CCGC 3 cut(s) 9, 32, 350
AcoI YGGCCR 1 cut(s) 6
AcvI CACGTG 1 cut(s) 84
AfaI GTAC 4 cut(s) 21, 59, 239, 296
AfiI CCNNNNNNNGG 1 cut(s) 38
AflIII ACRYGT 3 cut(s) 60, 81, 213
AjnI CCWGG 1 cut(s) 345
AleI CACNNNNGTG 1 cut(s) 82
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 213
Alw44I GTGCAC 1 cut(s) 209
AoxI GGCC 3 cut(s) 6, 41, 308
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 403
Asp718I GGTACC 1 cut(s) 237
AspS9I GGNCC 3 cut(s) 41, 309, 331
AsuC2I CCSGG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 237
BbrPI CACGTG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 347
BciVI GTATCC 1 cut(s) 346
BcnI CCSGG 1 cut(s) 242
BfaI CTAG 2 cut(s) 17, 38
BfmI CTRYAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 9, 32, 404
BlsI GCNGC 3 cut(s) 10, 33, 405
Bme1390I CCNGG 2 cut(s) 242, 347
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 41, 309, 331
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 2 cut(s) 242, 347
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 58, 120
BmuI ACTGGG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 242
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 265
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 3 cut(s) 73, 172, 183
BseLI CCNNNNNNNGG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 390
BsgI GTGCAG 1 cut(s) 74
BshFI GGCC 3 cut(s) 8, 43, 310
BshNI GGYRCC 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 3 cut(s) 44, 242, 320
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 3 cut(s) 8, 43, 310
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 3 cut(s) 9, 32, 350
BspANI GGCC 3 cut(s) 8, 43, 310
BspLI GGNNCC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 217
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 135
BstF5I GGATG 3 cut(s) 73, 172, 183
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 3 cut(s) 40, 134, 217
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 2 cut(s) 64, 217
BstSCI CCNGG 2 cut(s) 240, 345
BstSFI CTRYAG 1 cut(s) 407
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 390
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 3 cut(s) 8, 43, 310
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 3 cut(s) 73, 172, 183
Cac8I GCNNGC 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 3 cut(s) 41, 309, 331
Csp6I GTAC 4 cut(s) 20, 58, 238, 295
CviAII CATG 5 cut(s) 12, 61, 91, 138, 214
CviJI RGCY 8 cut(s) 8, 16, 31, 43, 310, 323, 340, 403
CviKI_1 RGCY 8 cut(s) 8, 16, 31, 43, 310, 323, 340, 403
CviQI GTAC 4 cut(s) 20, 58, 238, 295
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 331
Eco72I CACGTG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 5 cut(s) 15, 64, 94, 141, 217
FaiI YATR 8 cut(s) 13, 62, 92, 139, 173, 215, 251, 273
FatI CATG 5 cut(s) 11, 60, 90, 137, 213
Fnu4HI GCNGC 3 cut(s) 9, 32, 404
FokI GGATG 3 cut(s) 80, 179, 190
Fsp4HI GCNGC 3 cut(s) 9, 32, 404
FspBI CTAG 2 cut(s) 17, 38
GluI GCNGC 3 cut(s) 9, 32, 404
HaeIII GGCC 3 cut(s) 8, 43, 310
HapII CCGG 3 cut(s) 44, 242, 320
Hin1II CATG 5 cut(s) 15, 64, 94, 141, 217
HinfI GANTC 4 cut(s) 74, 265, 303, 362
HpaII CCGG 3 cut(s) 44, 242, 320
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 28, 120, 282, 367
Hpy188III TCNNGA 3 cut(s) 196, 256, 397
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 3 cut(s) 55, 211, 220
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 134, 217
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 5 cut(s) 15, 64, 94, 141, 217
KpnI GGTACC 1 cut(s) 241
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 2 cut(s) 29, 87
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 2 cut(s) 58, 120
MaeI CTAG 2 cut(s) 17, 38
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 1 cut(s) 109
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 2 cut(s) 109, 357
MlyI GAGTC 2 cut(s) 68, 356
MmeI TCCRAC 2 cut(s) 45, 144
MnlI CCTC 5 cut(s) 40, 87, 177, 344, 373
MslI CAYNNNNRTG 1 cut(s) 82
MspI CCGG 3 cut(s) 44, 242, 320
MspR9I CCNGG 2 cut(s) 242, 347
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 3 cut(s) 40, 134, 217
NciI CCSGG 1 cut(s) 242
NdeII GATC 1 cut(s) 120
NlaIII CATG 5 cut(s) 15, 64, 94, 141, 217
NlaIV GGNNCC 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 2 cut(s) 64, 217
OliI CACNNNNGTG 1 cut(s) 82
PciI ACATGT 2 cut(s) 60, 213
PciSI GCTCTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 265, 303
PkrI GCNGC 3 cut(s) 10, 33, 405
PleI GAGTC 2 cut(s) 68, 356
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
PpsI GAGTC 2 cut(s) 68, 356
Ppu21I YACGTR 1 cut(s) 84
PscI ACATGT 2 cut(s) 60, 213
Psp6I CCWGG 1 cut(s) 345
PspCI CACGTG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 3 cut(s) 41, 309, 331
RsaI GTAC 4 cut(s) 21, 59, 239, 296
RsaNI GTAC 4 cut(s) 20, 58, 238, 295
RseI CAYNNNNRTG 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 92
SatI GCNGC 3 cut(s) 9, 32, 404
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 3 cut(s) 41, 309, 331
SchI GAGTC 2 cut(s) 68, 356
ScrFI CCNGG 2 cut(s) 242, 347
SduI GDGCHC 1 cut(s) 213
SetI ASST 4 cut(s) 51, 86, 336, 405
SfaNI GCATC 2 cut(s) 58, 120
SfcI CTRYAG 1 cut(s) 407
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 82
Sse9I AATT 2 cut(s) 109, 357
SsiI CCGC 3 cut(s) 9, 32, 350
SspMI CTAG 2 cut(s) 17, 38
StyD4I CCNGG 2 cut(s) 240, 345
TaiI ACGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 387, 398
TaqII GACCGA 1 cut(s) 181
TasI AATT 2 cut(s) 109, 357
TatI WGTACW 1 cut(s) 57
TauI GCSGC 2 cut(s) 11, 34
TfiI GAWTC 2 cut(s) 265, 303
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 190
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 331
XceI RCATGY 2 cut(s) 64, 217
XspI CTAG 2 cut(s) 17, 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.