RLG00000018516

Multiple C2 and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007532
Physical Location & Seq
Reverse (-)
31182664 .. 31183074
411 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018516

Sequence Viewer

Length: 411 bp
ATGGTGGCCGCATGGCTAGCACGCTCCGAGCCGCCGCTAGGGCCGGAGGTGGTGCGGTACATGATGGATGCTGACTCACGCACGTGGAGCATGAGGAAGAGCAAAGCGAATTGGTTTCAGATCGTGGCGATGCTGGCGTGGTTGGTCGGGTTAGCGAAGTGGTTGGATGATATGCGGATGTGGAGGAACTCGGTGACGACGGTTTTGGTGCACATGTTGTATTTGGTTCTGGTTTGGTACCCGGATTTGATAATCCCGACTGGGTTTCTGTATGTGTTTTTGATTGGGGTTTGGTACCACCGATTCAGGCCCAAGATACCGACTGGAATGGACCTCTGGCTCTCCCAGGCAGATACAATCGACCCGGATAACCTTGATGAGGAGTTCGACACTTTCTTGAAGCTGCTGTAG

Protein Analysis

137

Amino Acids

16.07

Weight (kDa)

5.58

Isoelectric Point (pI)

26.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRT_C PF08372 86 - 132 4.1e-13 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 237, 294
AccB1I GGYRCC 2 cut(s) 237, 294
AciI CCGC 5 cut(s) 9, 32, 35, 55, 175
AcoI YGGCCR 1 cut(s) 6
AcvI CACGTG 1 cut(s) 84
AfaI GTAC 3 cut(s) 59, 239, 296
AfiI CCNNNNNNNGG 2 cut(s) 38, 379
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 1 cut(s) 345
AleI CACNNNNGTG 1 cut(s) 82
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 213
Alw44I GTGCAC 1 cut(s) 209
AoxI GGCC 3 cut(s) 6, 41, 308
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 403
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
Asp718I GGTACC 2 cut(s) 237, 294
AspS9I GGNCC 3 cut(s) 41, 309, 331
AsuC2I CCSGG 2 cut(s) 242, 365
AsuHPI GGTGA 1 cut(s) 205
AsuNHI GCTAGC 1 cut(s) 16
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 2 cut(s) 237, 294
BbrPI CACGTG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 390
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 1 cut(s) 347
BcnI CCSGG 2 cut(s) 242, 365
BfaI CTAG 2 cut(s) 17, 38
BfmI CTRYAG 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 4 cut(s) 9, 32, 35, 404
BlsI GCNGC 4 cut(s) 10, 33, 36, 405
Bme1390I CCNGG 3 cut(s) 242, 347, 365
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 41, 309, 331
BmiI GGNNCC 2 cut(s) 239, 296
BmrFI CCNGG 3 cut(s) 242, 347, 365
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 58, 120
BmtI GCTAGC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 270
BpuMI CCSGG 2 cut(s) 242, 365
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 379
Bse1I ACTGG 2 cut(s) 265, 328
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 3 cut(s) 73, 172, 183
BseLI CCNNNNNNNGG 2 cut(s) 38, 379
BseNI ACTGG 2 cut(s) 265, 328
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 390
BshFI GGCC 3 cut(s) 8, 43, 310
BshNI GGYRCC 2 cut(s) 237, 294
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 3 cut(s) 44, 242, 365
BslI CCNNNNNNNGG 2 cut(s) 38, 379
BsnI GGCC 3 cut(s) 8, 43, 310
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 5 cut(s) 9, 32, 35, 55, 175
BspANI GGCC 3 cut(s) 8, 43, 310
BspLI GGNNCC 2 cut(s) 239, 296
BspOI GCTAGC 1 cut(s) 20
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 2 cut(s) 237, 294
BsrI ACTGG 2 cut(s) 265, 328
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 18, 22, 135
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 3 cut(s) 73, 172, 183
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 4 cut(s) 17, 40, 87, 134
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 3 cut(s) 240, 345, 363
BstSFI CTRYAG 1 cut(s) 407
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 390
BsuRI GGCC 3 cut(s) 8, 43, 310
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 3 cut(s) 73, 172, 183
Cac8I GCNNGC 3 cut(s) 18, 22, 135
Cfr13I GGNCC 3 cut(s) 41, 309, 331
Csp6I GTAC 3 cut(s) 58, 238, 295
CviAII CATG 4 cut(s) 12, 61, 91, 214
CviJI RGCY 7 cut(s) 8, 16, 31, 43, 310, 340, 403
CviKI_1 RGCY 7 cut(s) 8, 16, 31, 43, 310, 340, 403
CviQI GTAC 3 cut(s) 58, 238, 295
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 331
Eco72I CACGTG 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 4 cut(s) 15, 64, 94, 217
FaiI YATR 6 cut(s) 13, 62, 92, 173, 215, 273
FatI CATG 4 cut(s) 11, 60, 90, 213
Fnu4HI GCNGC 4 cut(s) 9, 32, 35, 404
FokI GGATG 3 cut(s) 80, 179, 190
Fsp4HI GCNGC 4 cut(s) 9, 32, 35, 404
FspBI CTAG 2 cut(s) 17, 38
GluI GCNGC 4 cut(s) 9, 32, 35, 404
HaeIII GGCC 3 cut(s) 8, 43, 310
HapII CCGG 3 cut(s) 44, 242, 365
Hin1II CATG 4 cut(s) 15, 64, 94, 217
HinfI GANTC 2 cut(s) 74, 303
HpaII CCGG 3 cut(s) 44, 242, 365
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 28, 120
Hpy188III TCNNGA 2 cut(s) 256, 397
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 202
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 40, 87, 134
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 4 cut(s) 15, 64, 94, 217
KpnI GGTACC 2 cut(s) 241, 298
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 2 cut(s) 29, 87
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 2 cut(s) 58, 120
MaeI CTAG 2 cut(s) 17, 38
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 109
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 5 cut(s) 40, 87, 177, 344, 373
MslI CAYNNNNRTG 1 cut(s) 82
MspI CCGG 3 cut(s) 44, 242, 365
MspR9I CCNGG 3 cut(s) 242, 347, 365
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 17, 40, 87, 134
NciI CCSGG 2 cut(s) 242, 365
NdeII GATC 1 cut(s) 120
NheI GCTAGC 1 cut(s) 16
NlaIII CATG 4 cut(s) 15, 64, 94, 217
NlaIV GGNNCC 2 cut(s) 239, 296
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 1 cut(s) 217
OliI CACNNNNGTG 1 cut(s) 82
PciI ACATGT 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 303
PkrI GCNGC 4 cut(s) 10, 33, 36, 405
PleI GAGTC 1 cut(s) 68
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
PpsI GAGTC 1 cut(s) 68
Ppu21I YACGTR 1 cut(s) 84
PscI ACATGT 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 345
PspCI CACGTG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 239, 296
PspPI GGNCC 3 cut(s) 41, 309, 331
RsaI GTAC 3 cut(s) 59, 239, 296
RsaNI GTAC 3 cut(s) 58, 238, 295
RseI CAYNNNNRTG 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 92
SatI GCNGC 4 cut(s) 9, 32, 35, 404
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 3 cut(s) 41, 309, 331
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 3 cut(s) 242, 347, 365
SduI GDGCHC 1 cut(s) 213
SetI ASST 5 cut(s) 51, 86, 336, 375, 405
SfaNI GCATC 2 cut(s) 58, 120
SfcI CTRYAG 1 cut(s) 407
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 82
Sse9I AATT 1 cut(s) 109
SsiI CCGC 5 cut(s) 9, 32, 35, 55, 175
SspMI CTAG 2 cut(s) 17, 38
StyD4I CCNGG 3 cut(s) 240, 345, 363
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 360, 387
TasI AATT 1 cut(s) 109
TauI GCSGC 3 cut(s) 11, 34, 37
TfiI GAWTC 1 cut(s) 303
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 193
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 377
XceI RCATGY 1 cut(s) 217
XspI CTAG 2 cut(s) 17, 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.