Rh2DG654500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007532
Physical Location & Seq
Reverse (-)
87966334 .. 87966552
219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG654500.1

Sequence Viewer

Length: 219 bp
ATGGATCAATTGGTTAGTGGTTGGCGACAATTTGGAGTGTGGCTTGAGGTCTGGTGCGACCATGGTGGATTTGTGGGTAGAGTGTTGAGACCGGTGGTGTGGGAGAAGGGAGGCGTTTGGGATGAAGTGCAATATGGATCGGGACCTAGGCAGGGCGGAGCAGCGGCGATGCTGCTTGGTGCCTCCGGCCAATGGGTCGATGCTAGGTTAGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

72

Amino Acids

7.83

Weight (kDa)

5.59

Isoelectric Point (pI)

22.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AciI CCGC 2 cut(s) 156, 164
AclWI GGATC 2 cut(s) 12, 145
AcoI YGGCCR 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 152, 192
AgeI ACCGGT 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 2 cut(s) 12, 145
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 161, 172
AsiGI ACCGGT 1 cut(s) 91
AspA2I CCTAGG 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 143
AvaII GGWCC 1 cut(s) 143
AvrII CCTAGG 1 cut(s) 146
BanI GGYRCC 1 cut(s) 179
BbvI GCAGC 2 cut(s) 159, 173
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 2 cut(s) 147, 204
BisI GCNGC 3 cut(s) 162, 165, 173
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 3 cut(s) 163, 166, 174
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 144, 181
BmsI GCATC 2 cut(s) 159, 190
BpuEI CTTGAG 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 61, 146
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 192
Bse118I RCCGGY 1 cut(s) 91
BseDI CCNNGG 2 cut(s) 61, 146
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 152, 192
BseXI GCAGC 2 cut(s) 159, 173
BshFI GGCC 1 cut(s) 189
BshNI GGYRCC 1 cut(s) 179
BshTI ACCGGT 1 cut(s) 91
BsiSI CCGG 2 cut(s) 92, 186
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 2 cut(s) 152, 192
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 1 cut(s) 189
Bso31I GGTCTC 1 cut(s) 82
Bsp143I GATC 2 cut(s) 4, 137
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 2 cut(s) 156, 164
BspANI GGCC 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 144, 181
BspPI GGATC 2 cut(s) 12, 145
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 61, 146
BssMI GATC 2 cut(s) 4, 137
BssT1I CCWWGG 2 cut(s) 61, 146
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 2 cut(s) 7, 140
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 2 cut(s) 4, 137
BstV1I GCAGC 2 cut(s) 159, 173
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 182
BtsCI GGATG 1 cut(s) 127
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 143
CspAI ACCGGT 1 cut(s) 91
CviAII CATG 1 cut(s) 62
CviJI RGCY 2 cut(s) 43, 189
CviKI_1 RGCY 2 cut(s) 43, 189
DpnI GATC 2 cut(s) 6, 139
DpnII GATC 2 cut(s) 4, 137
EaeI YGGCCR 1 cut(s) 187
EciI GGCGGA 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 61, 146
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 61, 146
ErhI CCWWGG 2 cut(s) 61, 146
FaeI CATG 1 cut(s) 65
FaiI YATR 2 cut(s) 63, 135
FaqI GGGAC 1 cut(s) 156
FatI CATG 1 cut(s) 61
Fnu4HI GCNGC 3 cut(s) 162, 165, 173
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 162, 165, 173
FspBI CTAG 2 cut(s) 147, 204
GluI GCNGC 3 cut(s) 162, 165, 173
HaeIII GGCC 1 cut(s) 189
HapII CCGG 2 cut(s) 92, 186
Hin1II CATG 1 cut(s) 65
HpaII CCGG 2 cut(s) 92, 186
Hpy188III TCNNGA 1 cut(s) 141
HpyAV CCTTC 1 cut(s) 100
HpyCH4V TGCA 1 cut(s) 130
Hsp92II CATG 1 cut(s) 65
Kzo9I GATC 2 cut(s) 4, 137
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 4 cut(s) 37, 105, 137, 199
Lsp1109I GCAGC 2 cut(s) 159, 173
LweI GCATC 2 cut(s) 159, 190
MaeI CTAG 2 cut(s) 147, 204
MalI GATC 2 cut(s) 6, 139
MboI GATC 2 cut(s) 4, 137
MfeI CAATTG 1 cut(s) 8
MluCI AATT 2 cut(s) 8, 29
MnlI CCTC 4 cut(s) 40, 104, 193, 206
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 92, 186
MunI CAATTG 1 cut(s) 8
NcoI CCATGG 1 cut(s) 61
NdeII GATC 2 cut(s) 4, 137
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 2 cut(s) 144, 181
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 3 cut(s) 163, 166, 174
PpuMI RGGWCCY 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 144, 181
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
SatI GCNGC 3 cut(s) 162, 165, 173
Sau3AI GATC 2 cut(s) 4, 137
Sau96I GGNCC 1 cut(s) 143
SetI ASST 3 cut(s) 51, 148, 209
SfaNI GCATC 2 cut(s) 159, 190
SgeI CNNG 9 cut(s) 56, 64, 74, 104, 153, 159, 164, 188, 198
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 8, 29
SsiI CCGC 2 cut(s) 156, 164
SspMI CTAG 2 cut(s) 147, 204
StyI CCWWGG 2 cut(s) 61, 146
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 8, 29
TauI GCSGC 1 cut(s) 167
TseI GCWGC 2 cut(s) 161, 172
TspDTI ATGAA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 143
XmaJI CCTAGG 1 cut(s) 146
XspI CTAG 2 cut(s) 147, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.