RLG00000019217

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008174
Physical Location & Seq
Reverse (-)
48506289 .. 48509353
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019217

Sequence Viewer

Length: 411 bp
ATGGCTGAAGCTCTAGTTGACTTTCTCCAACAGTGTTTGGCTTCCATCACCCTTGACAAGGTTGAAAGAGAGCTGAGAGCGGTCGTAGGTGTTGACAAAGAAGCTGCAAACATCAACCACAATGCAGATCAGGAAAAAATAAAGGGATTAGGGAGGTCAACCCTCGAAGTTTTAATGACCGACTCCTTAAGACCAAATACCTTCAGCCTCAGCTACTCCTTAATCAAATTTTCTGTGCCAGCACTTTCCACTGAATTGAGAGTGTGCAATGATCTTCAGAGACATTTGAAAGCTGTTCTTTCTGTGGTTCATCTTGCTGTGCCTACCGGATCCTCCTCTGCTCTTCTATTCCCCAAAGTTCACCTGGTGATGTGGTGCTGGAAACCTGTACATCAATTGCACAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.11

Weight (kDa)

7.8

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 80
AciI CCGC 1 cut(s) 80
AclWI GGATC 2 cut(s) 324, 337
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 3 cut(s) 27, 187, 260
AdeI CACNNNGTG 1 cut(s) 367
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 1 cut(s) 58
AflII CTTAAG 1 cut(s) 187
AgsI TTSAA 2 cut(s) 65, 289
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 5 cut(s) 11, 73, 104, 213, 293
AluI AGCT 5 cut(s) 11, 73, 104, 213, 293
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 2 cut(s) 324, 337
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 1 cut(s) 227
AsuHPI GGTGA 3 cut(s) 40, 353, 379
BamHI GGATCC 1 cut(s) 329
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 91
BccI CCATC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 1 cut(s) 14
BfrI CTTAAG 1 cut(s) 187
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 365
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 2 cut(s) 65, 223
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 3 cut(s) 127, 271, 329
BspACI CCGC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 66, 222
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 2 cut(s) 324, 337
BspQI GCTCTTC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 187
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 388
BssMI GATC 3 cut(s) 127, 271, 329
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 348
BstAFI CTTAAG 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 2 cut(s) 74, 209
BstENI CCTNNNNNAGG 1 cut(s) 56
BstKTI GATC 3 cut(s) 130, 274, 332
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 3 cut(s) 127, 271, 329
BstMCI CGRYCG 1 cut(s) 84
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BtsIMutI CAGTG 2 cut(s) 38, 249
Cac8I GCNNGC 1 cut(s) 240
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 1 cut(s) 389
CviAII CATG 1 cut(s) 408
CviJI RGCY 8 cut(s) 5, 11, 41, 73, 104, 207, 213, 293
CviKI_1 RGCY 8 cut(s) 5, 11, 41, 73, 104, 207, 213, 293
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 2 cut(s) 74, 209
DpnI GATC 3 cut(s) 129, 273, 331
DpnII GATC 3 cut(s) 127, 271, 329
DraIII CACNNNGTG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco57I CTGAAG 3 cut(s) 27, 187, 260
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 363
FaeI CATG 1 cut(s) 411
FaiI YATR 1 cut(s) 409
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FatI CATG 1 cut(s) 407
Fnu4HI GCNGC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 1 cut(s) 14
GluI GCNGC 1 cut(s) 105
HapII CCGG 1 cut(s) 327
Hin1II CATG 1 cut(s) 411
HincII GTYRAC 3 cut(s) 19, 94, 159
HindII GTYRAC 3 cut(s) 19, 94, 159
HinfI GANTC 1 cut(s) 182
HpaII CCGG 1 cut(s) 327
HphI GGTGA 3 cut(s) 40, 353, 379
Hpy166II GTNNAC 4 cut(s) 19, 94, 159, 361
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 4 cut(s) 19, 94, 159, 361
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 4 cut(s) 107, 125, 267, 400
HpyF3I CTNAG 2 cut(s) 74, 209
Hsp92II CATG 1 cut(s) 411
Kzo9I GATC 3 cut(s) 127, 271, 329
LguI GCTCTTC 1 cut(s) 348
LpnPI CCDG 7 cut(s) 116, 252, 340, 350, 364, 377, 399
Lsp1109I GCAGC 1 cut(s) 91
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 1 cut(s) 14
MalI GATC 3 cut(s) 129, 273, 331
MbiI CCGCTC 1 cut(s) 80
MboI GATC 3 cut(s) 127, 271, 329
MboII GAAGA 2 cut(s) 266, 335
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 1 cut(s) 329
MluCI AATT 3 cut(s) 227, 254, 395
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 5 cut(s) 147, 173, 218, 343, 346
MseI TTAA 3 cut(s) 173, 188, 221
MspCI CTTAAG 1 cut(s) 187
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 365
NdeII GATC 3 cut(s) 127, 271, 329
NlaIII CATG 1 cut(s) 411
NlaIV GGNNCC 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 348
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 331
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 173, 188, 221
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 3 cut(s) 127, 271, 329
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 365
SexAI ACCWGGT 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 3 cut(s) 227, 254, 395
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 165
TaqII GACCGA 1 cut(s) 194
TasI AATT 3 cut(s) 227, 254, 395
TatI WGTACW 1 cut(s) 388
Tru1I TTAA 3 cut(s) 173, 188, 221
Tru9I TTAA 3 cut(s) 173, 188, 221
TscAI CASTG 2 cut(s) 38, 256
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 299
TspRI CASTG 2 cut(s) 38, 256
Vha464I CTTAAG 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.