Rorug02G0294200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008174
Physical Location & Seq
Reverse (-)
32502652 .. 32503229
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0294200.1

Sequence Viewer

Length: 408 bp
ATGGCTTGGTACGAGGAGTACCTACAGGCCAATACACCGATCAACACTTCCACATCTGGTAGTAAGAAAGTCAGCAAGCGTTGGCAAGCTCCTGGGGGAGAGACCTTGAAGCTAAACGTGGATGGCGCGTTTTTACCCAATGATCAATTTGGAGGTACAAGTGGTGTTCTTTGCAATGCTCAAGGTCAGTTTCTTGCTGCCTTTTCTCATCGGATGAACTTTGTCAGTTCTCCTCTACATGTGGAACTATTGGCTTTGAAGACTGGAGTAGAAATGATGCAAGCTAATGTCGTAGCTCATAGATTGGCTGGTCTTAGTTTTGAGTCTGATGTACAACTTGAATGGTTTGTAAACGCTCCAGATTTGATCTTGGATGCCCTTGTGTACGATCTTACTCGTACTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.83

Weight (kDa)

5.21

Isoelectric Point (pI)

23.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 39 - 100 5.2e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 128
AfaI GTAC 6 cut(s) 11, 20, 157, 333, 386, 400
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 3 cut(s) 109, 259, 341
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 4 cut(s) 89, 112, 284, 296
AluI AGCT 4 cut(s) 89, 112, 284, 296
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 1 cut(s) 197
AspLEI GCGC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 93
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 23
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 267, 364
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 2 cut(s) 285, 342
BpuEI CTTGAG 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 92
BseGI GGATG 3 cut(s) 127, 219, 379
BseMI GCAATG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 2 cut(s) 29, 222
BseXI GCAGC 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 4 cut(s) 39, 142, 366, 388
BspANI GGCC 1 cut(s) 29
BspFNI CGCG 1 cut(s) 128
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 4 cut(s) 39, 142, 366, 388
Bst2UI CCWGG 1 cut(s) 93
BstAUI TGTACA 1 cut(s) 331
BstC8I GCNNGC 3 cut(s) 77, 87, 282
BstDEI CTNAG 2 cut(s) 314, 402
BstF5I GGATG 3 cut(s) 127, 219, 379
BstFNI CGCG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 42, 145, 369, 391
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 4 cut(s) 39, 142, 366, 388
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 23
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 184
BstV2I GAAGAC 1 cut(s) 266
BsuRI GGCC 1 cut(s) 29
BtsCI GGATG 3 cut(s) 127, 219, 379
Cac8I GCNNGC 3 cut(s) 77, 87, 282
CfoI GCGC 1 cut(s) 128
Csp6I GTAC 6 cut(s) 10, 19, 156, 332, 385, 399
CviAII CATG 1 cut(s) 239
CviJI RGCY 8 cut(s) 5, 29, 89, 112, 254, 284, 296, 308
CviKI_1 RGCY 8 cut(s) 5, 29, 89, 112, 254, 284, 296, 308
CviQI GTAC 6 cut(s) 10, 19, 156, 332, 385, 399
DdeI CTNAG 2 cut(s) 314, 402
DpnI GATC 4 cut(s) 41, 144, 368, 390
DpnII GATC 4 cut(s) 39, 142, 366, 388
Eco31I GGTCTC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 242
FaiI YATR 2 cut(s) 240, 300
FatI CATG 1 cut(s) 238
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 3 cut(s) 134, 226, 386
Fsp4HI GCNGC 1 cut(s) 198
GlaI GCGC 1 cut(s) 127
GluI GCNGC 1 cut(s) 198
GsuI CTGGAG 2 cut(s) 285, 342
HaeIII GGCC 1 cut(s) 29
HhaI GCGC 1 cut(s) 128
Hin1II CATG 1 cut(s) 242
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 352, 385
Hpy188I TCNGA 2 cut(s) 213, 328
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 352, 385
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 174, 280
HpyF3I CTNAG 2 cut(s) 314, 402
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 1 cut(s) 242
HspAI GCGC 1 cut(s) 126
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 39, 142, 366, 388
LmnI GCTCC 2 cut(s) 94, 361
LpnPI CCDG 7 cut(s) 11, 42, 78, 105, 249, 294, 372
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 267, 364
MaeII ACGT 1 cut(s) 117
MalI GATC 4 cut(s) 41, 144, 368, 390
MboI GATC 4 cut(s) 39, 142, 366, 388
MboII GAAGA 1 cut(s) 271
MluCI AATT 1 cut(s) 146
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 3 cut(s) 7, 146, 243
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 128
NdeII GATC 4 cut(s) 39, 142, 366, 388
NlaIII CATG 1 cut(s) 242
NspI RCATGY 1 cut(s) 242
PciI ACATGT 1 cut(s) 238
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
RsaI GTAC 6 cut(s) 11, 20, 157, 333, 386, 400
RsaNI GTAC 6 cut(s) 10, 19, 156, 332, 385, 399
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 4 cut(s) 39, 142, 366, 388
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 93
SetI ASST 9 cut(s) 24, 91, 107, 114, 120, 157, 187, 286, 298
SfaNI GCATC 2 cut(s) 267, 364
SfcI CTRYAG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 91
TaiI ACGT 1 cut(s) 120
TasI AATT 1 cut(s) 146
TatI WGTACW 1 cut(s) 331
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 230
XceI RCATGY 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.