Rroxscaffold_2G00112560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008174
Physical Location & Seq
Forward (+)
37742736 .. 37764735
22000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00112560.1

Sequence Viewer

Length: 777 bp
ATGGCTCTAACCGGCGATCCGAGTTCGGTGGCGGTGTACGGTGCGGTTGCTAGTTTAGGAAGGTGGAGGCAGCGCGCCCTGCTAGAGATAGAGCAAGTCAAGGAATTGGTCAGCAAGAAAACGATGTTGAATGGTCATGACAATTTTAGAAAAGGCTGCAGGCATCCCTATGTGTATAAAAGTGGGAGGTCAGGCCTCAAACTCCCTAGACAAGGGATTGGGAGGAACCTCCAGGTTGTAATGGCTGGATTCCCTAAGACCGAATGCCCCCATCCTCAGACACTGAAATCTATTTTGGAATCCGTCGTCCGTAGAGGTGCTGGAAACCCTCAATTGTACAATACACATAGAACCAAAACTAAAACGGGAAGCAAAGTGAAAGAAACAACTGGAAAAATTAGTCGTGTCACTCGTATAGAGATCCAGAAGATCAGAGCTTCATATCTGTCCACCCTTGGTTATATTGGGTTTACAATGTCATTGGAATTTCAAGCTGATGGGTTAATATGTACTGTGTTCGGTAGGAGTGGTTGGTTGCCGAGGACGCCGTCGCCGGGTTTTGTCATCGGGCTAGTCGGGCCTTTTGCCTATGTGTGCACTCAAAGTGCCTTGTCTGCTCTAGGTAGGCGGCGAGTTCCTTGCTTCATCAAATGGTCGCTACCTCCTAGTAGCAGTGCAAAGTTGGCATCTCGTAAGATTGTTGAGACTCTGTTTCTTAGGATGGACATGATTGAGGTTATGCGAGTGAAGTTAACACACTTTTGGGATTCGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

28.72

Weight (kDa)

10.38

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 32, 44, 628
AclWI GGATC 2 cut(s) 11, 415
AcsI RAATTY 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 545
AfaI GTAC 3 cut(s) 38, 338, 511
AfiI CCNNNNNNNGG 2 cut(s) 212, 554
AgsI TTSAA 2 cut(s) 130, 491
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 2 cut(s) 437, 494
AluI AGCT 2 cut(s) 437, 494
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 1 cut(s) 698
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 2 cut(s) 11, 415
AlwNI CAGNNNCTG 1 cut(s) 283
AoxI GGCC 2 cut(s) 193, 578
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 2 cut(s) 70, 156
ApoI RAATTY 1 cut(s) 485
AspLEI GCGC 2 cut(s) 75, 77
AspS9I GGNCC 1 cut(s) 578
AsuC2I CCSGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 82, 143
BccI CCATC 3 cut(s) 279, 491, 715
BceAI ACGGC 1 cut(s) 532
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 7 cut(s) 51, 83, 207, 572, 620, 666, 775
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 3 cut(s) 71, 157, 629
BlsI GCNGC 3 cut(s) 72, 158, 630
Bme1390I CCNGG 2 cut(s) 233, 555
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 2 cut(s) 233, 555
BmsI GCATC 2 cut(s) 172, 695
BpmI CTGGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 454, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 554
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 394
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 454, 539
BseGI GGATG 3 cut(s) 163, 271, 726
BseLI CCNNNNNNNGG 2 cut(s) 212, 554
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 1 cut(s) 394
BsePI GCGCGC 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 2 cut(s) 82, 143
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 195, 580
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 2 cut(s) 12, 554
BslI CCNNNNNNNGG 2 cut(s) 212, 554
BsmAI GTCTC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 2 cut(s) 195, 580
Bsp1286I GDGCHC 1 cut(s) 599
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 3 cut(s) 16, 420, 429
BspACI CCGC 3 cut(s) 32, 44, 628
BspANI GGCC 2 cut(s) 195, 580
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 227
BspMAI CTGCAG 1 cut(s) 161
BspPI GGATC 2 cut(s) 11, 415
BsrFI RCCGGY 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 454, 539
BssHII GCGCGC 1 cut(s) 73
BssMI GATC 3 cut(s) 16, 420, 429
BssNI GRCGYC 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 41, 514
BstACI GRCGYC 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 75, 161
BstDEI CTNAG 3 cut(s) 255, 276, 716
BstENI CCTNNNNNAGG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 163, 271, 726
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 75, 77
BstKTI GATC 3 cut(s) 19, 423, 432
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 3 cut(s) 16, 420, 429
BstMWI GCNNNNNNNGC 5 cut(s) 79, 544, 577, 614, 683
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 231, 553
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 82, 143
BstX2I RGATCY 1 cut(s) 420
BstYI RGATCY 1 cut(s) 420
BsuRI GGCC 2 cut(s) 195, 580
BtsCI GGATG 3 cut(s) 163, 271, 726
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 2 cut(s) 281, 679
Cac8I GCNNGC 2 cut(s) 75, 161
CaiI CAGNNNCTG 1 cut(s) 283
CciI TCATGA 1 cut(s) 136
CfoI GCGC 2 cut(s) 75, 77
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 1 cut(s) 553
Csp6I GTAC 3 cut(s) 37, 337, 510
CviAII CATG 2 cut(s) 137, 727
CviJI RGCY 8 cut(s) 5, 156, 195, 245, 437, 494, 571, 580
CviKI_1 RGCY 8 cut(s) 5, 156, 195, 245, 437, 494, 571, 580
CviQI GTAC 3 cut(s) 37, 337, 510
DdeI CTNAG 3 cut(s) 255, 276, 716
DpnI GATC 3 cut(s) 18, 422, 431
DpnII GATC 3 cut(s) 16, 420, 429
Eco130I CCWWGG 1 cut(s) 454
Eco147I AGGCCT 1 cut(s) 195
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 2 cut(s) 140, 730
FatI CATG 2 cut(s) 136, 726
Fnu4HI GCNGC 3 cut(s) 71, 157, 629
FokI GGATG 3 cut(s) 150, 258, 733
Fsp4HI GCNGC 3 cut(s) 71, 157, 629
FspBI CTAG 7 cut(s) 51, 83, 207, 572, 620, 666, 775
GlaI GCGC 2 cut(s) 74, 76
GluI GCNGC 3 cut(s) 71, 157, 629
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 2 cut(s) 195, 580
HapII CCGG 2 cut(s) 12, 554
HgaI GACGC 1 cut(s) 553
HhaI GCGC 2 cut(s) 75, 77
Hin1I GRCGYC 1 cut(s) 545
Hin1II CATG 2 cut(s) 140, 730
Hin6I GCGC 2 cut(s) 73, 75
HinP1I GCGC 2 cut(s) 73, 75
HincII GTYRAC 1 cut(s) 753
HindII GTYRAC 1 cut(s) 753
HinfI GANTC 4 cut(s) 249, 299, 706, 767
HpaI GTTAAC 1 cut(s) 753
HpaII CCGG 2 cut(s) 12, 554
Hpy166II GTNNAC 5 cut(s) 37, 450, 471, 597, 753
Hpy188I TCNGA 4 cut(s) 21, 279, 434, 772
Hpy188III TCNNGA 2 cut(s) 137, 424
Hpy8I GTNNAC 5 cut(s) 37, 450, 471, 597, 753
Hpy99I CGWCG 2 cut(s) 308, 553
HpyAV CCTTC 1 cut(s) 54
HpyCH4III ACNGT 2 cut(s) 41, 514
HpyCH4V TGCA 3 cut(s) 159, 597, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 544, 577, 614, 683
HpyF3I CTNAG 3 cut(s) 255, 276, 716
Hsp92I GRCGYC 1 cut(s) 545
Hsp92II CATG 2 cut(s) 140, 730
HspAI GCGC 2 cut(s) 73, 75
KspAI GTTAAC 1 cut(s) 753
Kzo9I GATC 3 cut(s) 16, 420, 429
Lsp1109I GCAGC 2 cut(s) 82, 143
LweI GCATC 2 cut(s) 172, 695
MaeI CTAG 7 cut(s) 51, 83, 207, 572, 620, 666, 775
MaeIII GTNAC 1 cut(s) 406
MalI GATC 3 cut(s) 18, 422, 431
MboI GATC 3 cut(s) 16, 420, 429
MboII GAAGA 1 cut(s) 439
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 599
MluCI AATT 5 cut(s) 104, 142, 332, 396, 485
MlyI GAGTC 1 cut(s) 700
MseI TTAA 2 cut(s) 503, 752
MslI CAYNNNNRTG 1 cut(s) 168
MspI CCGG 2 cut(s) 12, 554
MspR9I CCNGG 2 cut(s) 233, 555
MunI CAATTG 1 cut(s) 332
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 5 cut(s) 79, 544, 577, 614, 683
NciI CCSGG 1 cut(s) 555
NdeII GATC 3 cut(s) 16, 420, 429
NlaIII CATG 2 cut(s) 140, 730
NlaIV GGNNCC 1 cut(s) 227
NmeAIII GCCGAG 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 406
PagI TCATGA 1 cut(s) 136
PauI GCGCGC 1 cut(s) 73
PceI AGGCCT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 409
PctI GAATGC 1 cut(s) 269
PfeI GAWTC 3 cut(s) 249, 299, 767
PflFI GACNNNGTC 1 cut(s) 547
PkrI GCNGC 3 cut(s) 72, 158, 630
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 578
PstI CTGCAG 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 420
PsyI GACNNNGTC 1 cut(s) 547
PteI GCGCGC 1 cut(s) 73
RsaI GTAC 3 cut(s) 38, 338, 511
RsaNI GTAC 3 cut(s) 37, 337, 510
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 2 cut(s) 503, 752
SatI GCNGC 3 cut(s) 71, 157, 629
Sau3AI GATC 3 cut(s) 16, 420, 429
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 2 cut(s) 233, 555
SduI GDGCHC 1 cut(s) 599
SfaNI GCATC 2 cut(s) 172, 695
SfcI CTRYAG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 168
Sse9I AATT 5 cut(s) 104, 142, 332, 396, 485
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 3 cut(s) 32, 44, 628
SspMI CTAG 7 cut(s) 51, 83, 207, 572, 620, 666, 775
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 231, 553
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 2 cut(s) 41, 514
TaqII GACCGA 1 cut(s) 275
TasI AATT 5 cut(s) 104, 142, 332, 396, 485
TatI WGTACW 2 cut(s) 336, 509
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 3 cut(s) 249, 299, 767
Tru1I TTAA 2 cut(s) 503, 752
Tru9I TTAA 2 cut(s) 503, 752
TscAI CASTG 2 cut(s) 288, 679
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 2 cut(s) 70, 156
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 429, 634
TspGWI ACGGA 2 cut(s) 292, 299
TspRI CASTG 2 cut(s) 288, 679
Tth111I GACNNNGTC 1 cut(s) 547
VneI GTGCAC 1 cut(s) 595
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 1 cut(s) 485
XspI CTAG 7 cut(s) 51, 83, 207, 572, 620, 666, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.