RLG00000027380

Belongs to the universal ribosomal protein uS8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003734
Physical Location & Seq
Reverse (-)
11100074 .. 11102772
2699 bp
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UTR
Exon/CDS
Intron
RLM00000027380

Sequence Viewer

Length: 588 bp
ATGGCAAATATTTCTATGGTCTTATTCGGGAAGAAGCCGCTGATACTAGTCGTGCACTTTTGTCGCCCAGAAGATGCAGCTTCGGCAGAAAATGCAAACATGGACAGAAGCAACAACAGAAGAGATATAAAAACCCTAACTCTCGGAGAGTCGGAGGCGCCTCTGCTTAGCTTGCTCATCTCATTCGCAGCAACAATGGTGAGAGTTAGCGTGCTGAATGATGCTCTCAAGAGCATGTACAATGCAGAGAAACGTGGTAAGCGTCAGGTGATGATCAGGCCATCATCCAAAGTGATCATCAAGTTCCTTATTGTCATGCAAAAGCATGGATACATCGGAGAGTTCGAGTATGTTGACGACCACAGGTCTGGTAAAATTGTGGTCGAACTGAATGGAAGGCTTAACAAGTGTGGGGTCATTAGTCCTCGTTTTGATGTTGGTGTTAAGGAGATTGAAGGTTGGACTGCCAGGTTGCTTCCTTCAAGACAGTTTGGATATATCGTGCTCACCACTTCTGCTGGCATCATGGACCATGAAGAGGCTAGAAGAAAGAATGTCGGTGGGAAAGTTCTTGGTTTCTTTTATTAG

Protein Analysis

196

Amino Acids

21.88

Weight (kDa)

9.69

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 72 - 195 6.9e-21 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AciI CCGC 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 538
AgsI TTSAA 2 cut(s) 455, 483
AhdI GACNNNNNGTC 1 cut(s) 364
AhlI ACTAGT 1 cut(s) 46
AjnI CCWGG 1 cut(s) 467
AluBI AGCT 2 cut(s) 80, 171
AluI AGCT 2 cut(s) 80, 171
Alw21I GWGCWC 2 cut(s) 57, 507
Alw44I GTGCAC 1 cut(s) 53
AoxI GGCC 1 cut(s) 278
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 77, 188
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 529
AsuHPI GGTGA 3 cut(s) 211, 280, 499
AvaII GGWCC 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 57
BanI GGYRCC 1 cut(s) 157
Bbv12I GWGCWC 2 cut(s) 57, 507
BbvI GCAGC 2 cut(s) 89, 200
BccI CCATC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 1 cut(s) 469
BciVI GTATCC 1 cut(s) 323
BclI TGATCA 2 cut(s) 273, 294
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 2 cut(s) 47, 543
BfoI RGCGCY 1 cut(s) 161
BfuI GTATCC 1 cut(s) 323
BisI GCNGC 3 cut(s) 38, 78, 189
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 3 cut(s) 39, 79, 190
Bme1390I CCNGG 1 cut(s) 469
Bme18I GGWCC 1 cut(s) 529
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 1 cut(s) 469
BmsI GCATC 3 cut(s) 64, 211, 531
Bpu1102I GCTNAGC 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 538
BseBI CCWGG 1 cut(s) 469
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 1 cut(s) 538
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 2 cut(s) 89, 200
BshFI GGCC 1 cut(s) 280
BshNI GGYRCC 1 cut(s) 157
BsiHKAI GWGCWC 2 cut(s) 57, 507
BslI CCNNNNNNNGG 1 cut(s) 538
BsnI GGCC 1 cut(s) 280
Bsp1286I GDGCHC 2 cut(s) 57, 507
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 2 cut(s) 273, 294
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 237
BssMI GATC 2 cut(s) 273, 294
BssNI GRCGYC 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 115, 531
BstACI GRCGYC 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 173, 212, 520
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 276, 297
BstMBI GATC 2 cut(s) 273, 294
BstMWI GCNNNNNNNGC 3 cut(s) 83, 92, 172
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 467
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 89, 200
BstXI CCANNNNNNTGG 1 cut(s) 368
BsuI GTATCC 1 cut(s) 323
BsuRI GGCC 1 cut(s) 280
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 3 cut(s) 173, 212, 520
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 529
CseI GACGC 1 cut(s) 251
Csp6I GTAC 1 cut(s) 238
CviAII CATG 6 cut(s) 100, 235, 316, 326, 526, 533
CviJI RGCY 6 cut(s) 37, 80, 171, 280, 400, 542
CviKI_1 RGCY 6 cut(s) 37, 80, 171, 280, 400, 542
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 167
DinI GGCGCC 1 cut(s) 159
DpnI GATC 2 cut(s) 275, 296
DpnII GATC 2 cut(s) 273, 294
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 115, 531
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 2 cut(s) 115, 531
Eco47I GGWCC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 467
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
FaeI CATG 6 cut(s) 103, 238, 319, 329, 529, 536
FatI CATG 6 cut(s) 99, 234, 315, 325, 525, 532
FbaI TGATCA 2 cut(s) 273, 294
Fnu4HI GCNGC 3 cut(s) 38, 78, 189
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 3 cut(s) 38, 78, 189
FspBI CTAG 2 cut(s) 47, 543
GlaI GCGC 1 cut(s) 159
GluI GCNGC 3 cut(s) 38, 78, 189
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 1 cut(s) 280
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 6 cut(s) 103, 238, 319, 329, 529, 536
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 1 cut(s) 149
HphI GGTGA 3 cut(s) 211, 280, 499
Hpy166II GTNNAC 2 cut(s) 55, 355
Hpy188I TCNGA 3 cut(s) 146, 154, 338
Hpy188III TCNNGA 3 cut(s) 28, 229, 483
Hpy8I GTNNAC 2 cut(s) 55, 355
HpyAV CCTTC 3 cut(s) 390, 449, 489
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 5 cut(s) 55, 77, 95, 245, 319
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 92, 172
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 6 cut(s) 103, 238, 319, 329, 529, 536
HspAI GCGC 1 cut(s) 158
KasI GGCGCC 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 273, 294
Kzo9I GATC 2 cut(s) 273, 294
LpnPI CCDG 8 cut(s) 81, 251, 262, 349, 354, 454, 481, 504
Lsp1109I GCAGC 2 cut(s) 89, 200
LweI GCATC 3 cut(s) 64, 211, 531
MaeI CTAG 2 cut(s) 47, 543
MaeII ACGT 1 cut(s) 253
MalI GATC 2 cut(s) 275, 296
MboI GATC 2 cut(s) 273, 294
MboII GAAGA 5 cut(s) 43, 83, 132, 548, 558
MhlI GDGCHC 2 cut(s) 57, 507
MluCI AATT 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 158
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 2 cut(s) 132, 440
MnlI CCTC 4 cut(s) 148, 171, 435, 532
MseI TTAA 2 cut(s) 402, 444
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 469
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 83, 92, 172
NarI GGCGCC 1 cut(s) 158
NdeII GATC 2 cut(s) 273, 294
NlaIII CATG 6 cut(s) 103, 238, 319, 329, 529, 536
NlaIV GGNNCC 1 cut(s) 159
NspI RCATGY 1 cut(s) 238
PcsI WCGNNNNNNNCGW 2 cut(s) 259, 342
PkrI GCNGC 3 cut(s) 39, 79, 190
PleI GAGTC 1 cut(s) 157
PluTI GGCGCC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 529
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 402, 444
SatI GCNGC 3 cut(s) 38, 78, 189
Sau3AI GATC 2 cut(s) 273, 294
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 469
SduI GDGCHC 2 cut(s) 57, 507
SetI ASST 7 cut(s) 82, 173, 256, 270, 368, 460, 473
SfaNI GCATC 3 cut(s) 64, 211, 531
SfoI GGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SpeI ACTAGT 1 cut(s) 46
Sse9I AATT 1 cut(s) 375
SsiI CCGC 1 cut(s) 38
SspDI GGCGCC 1 cut(s) 157
SspI AATATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 47, 543
StyD4I CCNGG 1 cut(s) 467
TaaI ACNGT 1 cut(s) 489
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 345, 384
TasI AATT 1 cut(s) 375
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 402, 444
Tru9I TTAA 2 cut(s) 402, 444
TseI GCWGC 2 cut(s) 77, 188
TspDTI ATGAA 1 cut(s) 549
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 529
XceI RCATGY 1 cut(s) 238
XspI CTAG 2 cut(s) 47, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.