Rroxscaffold_6G00400830

Belongs to the universal ribosomal protein uS8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003734
Physical Location & Seq
Reverse (-)
23029972 .. 23035567
5596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400830.1

Sequence Viewer

Length: 753 bp
ATGGGTTCGGATTTGAAATTGGAAGGCACATCAGCTCTGCATATACAACAAGGGTTTTGTATACTAATTAATTTCACTTCTGCACTCTCTCAGATAGAAATGGGGAAAACTAACATGGGTGTTGGTGGTTGCTGTAAAAAGATAGTTGGACTGGCGGGTTGTTGTGTGAATATGGATGAAGTTAGGAACTTGGCATCAGCGACAATGGTGAGAGTTAGCGTGCTGAATGATGCTCTCAAGAGCATGTACAATGCAGAGAAACGTGGTAAGCGTCAGGTGATGATCAGGCCGTCATCCAAAGTGATCATCAAGTTTCTTATTGTCATGCAAAAGCATGGATACATTGGAGAGTTCGAGTATGTTGACGACCACAGCTCTGGCAAAATTGTGGTCGAACTGAATGGAAGGCTTAAAAAGTGTGGGGTCATTAGTTCTAGTTTTGATGTTGGTGTTAAGGAGATTGAAGGTTGGACTGCCAGGTTGCTTCCTTCAAGACAGTTTGGATATATCGTGCTCACCACTTCTGCTGGAATCATGGACCATGAAGAGGCTAGAAGAAAGAATGTTATTTCTTCGAGATGTTCCATATTATGTTTGCACAAGTGGGAGATTGTTAGGCAGTGGTTCCAACCTGTAGAGGCTTCAAGGTTGAGAAGTAAAATCTGGAAGTTACTCAAGGAAGAATTTCAAAATGGATCCAAGTCTAACTGCTCTAAAGAATCTTCTCCTTTGAGTGATGCTTCTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S8

Protein Analysis

250

Amino Acids

27.83

Weight (kDa)

9.12

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 75 - 189 2.7e-16 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 61
AciI CCGC 1 cut(s) 155
AclWI GGATC 2 cut(s) 690, 703
AcsI RAATTY 1 cut(s) 683
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 5 cut(s) 16, 464, 492, 645, 689
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 2 cut(s) 35, 375
AluI AGCT 2 cut(s) 35, 375
Alw21I GWGCWC 1 cut(s) 516
AlwI GGATC 2 cut(s) 690, 703
AoxI GGCC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 683
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 684
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 3 cut(s) 220, 289, 508
AvaII GGWCC 1 cut(s) 538
BamHI GGATCC 1 cut(s) 695
Bbv12I GWGCWC 1 cut(s) 516
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 478
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 2 cut(s) 282, 303
BfaI CTAG 2 cut(s) 435, 552
BfmI CTRYAG 1 cut(s) 633
BfuI GTATCC 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 2 cut(s) 626, 697
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 3 cut(s) 203, 220, 727
BpuEI CTTGAG 2 cut(s) 221, 659
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 156
BseBI CCWGG 1 cut(s) 478
BseGI GGATG 2 cut(s) 181, 293
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 516
BslI CCNNNNNNNGG 1 cut(s) 547
BsnI GGCC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 516
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 3 cut(s) 282, 303, 695
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 626, 697
BspPI GGATC 2 cut(s) 690, 703
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 1 cut(s) 156
BssMI GATC 3 cut(s) 282, 303, 695
BssNAI GTATAC 1 cut(s) 62
Bst1107I GTATAC 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 498
Bst6I CTCTTC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 181, 293
BstKTI GATC 3 cut(s) 285, 306, 698
BstMBI GATC 3 cut(s) 282, 303, 695
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 695
BstZ17I GTATAC 1 cut(s) 62
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 1 cut(s) 289
BtsCI GGATG 2 cut(s) 181, 293
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 1 cut(s) 626
Cac8I GCNNGC 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 538
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 247
CviAII CATG 6 cut(s) 115, 244, 325, 335, 535, 542
CviJI RGCY 6 cut(s) 35, 289, 375, 409, 551, 641
CviKI_1 RGCY 6 cut(s) 35, 289, 375, 409, 551, 641
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 1 cut(s) 90
DpnI GATC 3 cut(s) 284, 305, 697
DpnII GATC 3 cut(s) 282, 303, 695
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 476
FaeI CATG 6 cut(s) 118, 247, 328, 338, 538, 545
FatI CATG 6 cut(s) 114, 243, 324, 334, 534, 541
FauI CCCGC 1 cut(s) 148
FbaI TGATCA 2 cut(s) 282, 303
FblI GTMKAC 1 cut(s) 61
FokI GGATG 2 cut(s) 188, 280
FspBI CTAG 2 cut(s) 435, 552
HaeIII GGCC 1 cut(s) 289
HgaI GACGC 1 cut(s) 260
Hin1II CATG 6 cut(s) 118, 247, 328, 338, 538, 545
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 531, 719
HphI GGTGA 3 cut(s) 220, 289, 508
Hpy166II GTNNAC 2 cut(s) 62, 364
Hpy188I TCNGA 3 cut(s) 10, 93, 745
Hpy188III TCNNGA 4 cut(s) 238, 492, 576, 664
Hpy8I GTNNAC 2 cut(s) 62, 364
HpyAV CCTTC 4 cut(s) 17, 399, 458, 498
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4IV ACGT 1 cut(s) 262
HpyCH4V TGCA 5 cut(s) 40, 83, 254, 328, 598
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 262
Hsp92II CATG 6 cut(s) 118, 247, 328, 338, 538, 545
Ksp22I TGATCA 2 cut(s) 282, 303
Kzo9I GATC 3 cut(s) 282, 303, 695
LpnPI CCDG 9 cut(s) 137, 260, 271, 363, 463, 490, 513, 645, 649
LweI GCATC 3 cut(s) 203, 220, 727
MaeI CTAG 2 cut(s) 435, 552
MaeII ACGT 1 cut(s) 262
MaeIII GTNAC 1 cut(s) 669
MalI GATC 3 cut(s) 284, 305, 697
MboI GATC 3 cut(s) 282, 303, 695
MboII GAAGA 5 cut(s) 557, 564, 567, 692, 714
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 5 cut(s) 17, 66, 70, 384, 683
MmeI TCCRAC 3 cut(s) 127, 449, 652
MnlI CCTC 2 cut(s) 541, 631
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 3 cut(s) 69, 411, 453
MspR9I CCNGG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 478
NdeII GATC 3 cut(s) 282, 303, 695
NlaIII CATG 6 cut(s) 118, 247, 328, 338, 538, 545
NlaIV GGNNCC 2 cut(s) 626, 697
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 2 cut(s) 531, 719
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 2 cut(s) 626, 697
PspPI GGNCC 1 cut(s) 538
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SaqAI TTAA 3 cut(s) 69, 411, 453
Sau3AI GATC 3 cut(s) 282, 303, 695
Sau96I GGNCC 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 1 cut(s) 516
SetI ASST 8 cut(s) 37, 265, 279, 377, 469, 482, 634, 650
SfaNI GCATC 3 cut(s) 203, 220, 727
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 538
SmlI CTYRAG 2 cut(s) 236, 674
SmoI CTYRAG 2 cut(s) 236, 674
Sse9I AATT 5 cut(s) 17, 66, 70, 384, 683
SsiI CCGC 1 cut(s) 155
SspMI CTAG 2 cut(s) 435, 552
StyD4I CCNGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 498
TaiI ACGT 1 cut(s) 265
TaqI TCGA 3 cut(s) 354, 393, 575
TasI AATT 5 cut(s) 17, 66, 70, 384, 683
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 2 cut(s) 531, 719
Tru1I TTAA 3 cut(s) 69, 411, 453
Tru9I TTAA 3 cut(s) 69, 411, 453
TscAI CASTG 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 192, 558
TspRI CASTG 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 538
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 1 cut(s) 683
XceI RCATGY 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 2 cut(s) 435, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.