Rmu_sc0000195.1_g000029

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000195.1
OG0003208
Physical Location & Seq
Reverse (-)
98565 .. 100274
1710 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
atgcgtgtgcatgcctgcatatacatagctagcttagccagtagagccagaagagctatcacctttgtcaatggtcgtaagcatccttcatccttgggcgttcgtgttgtcctctctcgcatccaaaactcccttcaactttccctaaccagagaagcagaaccagtaatcatgccgaagaacaagggaaagggaggaaagaacaggaagagaggaaagaacgaggctgatgacgaaaagcgtgagcttgtcttcaaggaagatggacaggagtatgcccaagtgcttcggatgctgggtaatggtcggtgcgaagccatgtgcattgatggaaccaagcgactttgccatatccgtggtaagatgcacaagaaggtttggattgcagctggggatatcattcttgttgggcttcgtgactatcaggatgacaaggccgatgtgattctcaagtacatgcctgatgaggctaggctgctgaaggcttatggagagcttccagagaatacacgtcttaacgagggtattactgggggtcttgatgaggaggaggaaggtggtgctgatgactacatcgagtttgaagatgaggatattgataagatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

202

Amino Acids

22.79

Weight (kDa)

6.92

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 89 - 150 6.2e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 3 cut(s) 137, 256, 584
AjiI CACGTC 1 cut(s) 512
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
AluBI AGCT 6 cut(s) 29, 33, 56, 247, 389, 496
AluI AGCT 6 cut(s) 29, 33, 56, 247, 389, 496
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 386, 475
AsuHPI GGTGA 1 cut(s) 52
AsuNHI GCTAGC 1 cut(s) 29
BarI GAAGNNNNNNTAC 2 cut(s) 70, 102
BbsI GAAGAC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 398, 462
BccI CCATC 2 cut(s) 257, 323
BfaI CTAG 2 cut(s) 30, 471
BisI GCNGC 2 cut(s) 387, 476
BlpI GCTNAGC 1 cut(s) 34
BlsI GCNGC 2 cut(s) 388, 477
BmgBI CACGTC 1 cut(s) 512
BmiI GGNNCC 1 cut(s) 334
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 4 cut(s) 91, 129, 282, 354
BmtI GCTAGC 1 cut(s) 33
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 244
Bpu1102I GCTNAGC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 93, 355
Bse1I ACTGG 3 cut(s) 39, 164, 535
BseDI CCNNGG 2 cut(s) 93, 355
BseGI GGATG 5 cut(s) 82, 89, 120, 297, 433
BseNI ACTGG 3 cut(s) 39, 164, 535
BseRI GAGGAG 2 cut(s) 560, 563
BseXI GCAGC 2 cut(s) 398, 462
BseYI CCCAGC 2 cut(s) 295, 389
BshFI GGCC 1 cut(s) 437
BsnI GGCC 1 cut(s) 437
Bsp1720I GCTNAGC 1 cut(s) 34
BspANI GGCC 1 cut(s) 437
BspLI GGNNCC 1 cut(s) 334
BspOI GCTAGC 1 cut(s) 33
BspQI GCTCTTC 1 cut(s) 46
BsrI ACTGG 3 cut(s) 39, 164, 535
BssECI CCNNGG 2 cut(s) 93, 355
BssT1I CCWWGG 1 cut(s) 93
Bst6I CTCTTC 2 cut(s) 46, 203
BstC8I GCNNGC 3 cut(s) 12, 16, 31
BstDEI CTNAG 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 5 cut(s) 82, 89, 120, 297, 433
BstMWI GCNNNNNNNGC 4 cut(s) 35, 44, 53, 292
BstNSI RCATGY 2 cut(s) 14, 460
BstV1I GCAGC 2 cut(s) 398, 462
BstV2I GAAGAC 1 cut(s) 244
BstXI CCANNNNNNTGG 1 cut(s) 356
BsuRI GGCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 355
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 5 cut(s) 82, 89, 120, 297, 433
Cac8I GCNNGC 3 cut(s) 12, 16, 31
Csp6I GTAC 1 cut(s) 454
CviAII CATG 4 cut(s) 11, 172, 319, 457
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 46, 203
EarI CTCTTC 2 cut(s) 46, 203
Eco130I CCWWGG 1 cut(s) 93
Eco32I GATATC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 500
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 14, 175, 322, 460
FatI CATG 4 cut(s) 10, 171, 318, 456
Fnu4HI GCNGC 2 cut(s) 387, 476
FokI GGATG 5 cut(s) 69, 76, 107, 304, 440
Fsp4HI GCNGC 2 cut(s) 387, 476
FspBI CTAG 2 cut(s) 30, 471
GluI GCNGC 2 cut(s) 387, 476
GsaI CCCAGC 2 cut(s) 299, 393
HaeIII GGCC 1 cut(s) 437
Hin1II CATG 4 cut(s) 14, 175, 322, 460
HinfI GANTC 1 cut(s) 445
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 4 cut(s) 416, 425, 500, 539
HpyAV CCTTC 5 cut(s) 96, 143, 367, 475, 548
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 5 cut(s) 10, 18, 324, 367, 386
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 44, 53, 292
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 4 cut(s) 14, 175, 322, 460
LguI GCTCTTC 1 cut(s) 46
Lsp1109I GCAGC 2 cut(s) 398, 462
LweI GCATC 4 cut(s) 91, 129, 282, 354
MaeI CTAG 2 cut(s) 30, 471
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 1 cut(s) 416
MboII GAAGA 6 cut(s) 63, 190, 220, 244, 272, 596
MseI TTAA 2 cut(s) 516, 607
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 389
MwoI GCNNNNNNNGC 4 cut(s) 35, 44, 53, 292
NheI GCTAGC 1 cut(s) 29
NlaIII CATG 4 cut(s) 14, 175, 322, 460
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 2 cut(s) 14, 460
PaeI GCATGC 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 445
PkrI GCNGC 2 cut(s) 388, 477
PspFI CCCAGC 2 cut(s) 295, 389
PspN4I GGNNCC 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 354
SapI GCTCTTC 1 cut(s) 46
SaqAI TTAA 2 cut(s) 516, 607
SatI GCNGC 2 cut(s) 387, 476
SfaNI GCATC 4 cut(s) 91, 129, 282, 354
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SphI GCATGC 1 cut(s) 14
SspMI CTAG 2 cut(s) 30, 471
StyI CCWWGG 1 cut(s) 93
TaiI ACGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 576
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 1 cut(s) 445
Tru1I TTAA 2 cut(s) 516, 607
Tru9I TTAA 2 cut(s) 516, 607
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 386, 475
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 78
TspGWI ACGGA 1 cut(s) 344
XceI RCATGY 2 cut(s) 14, 460
XspI CTAG 2 cut(s) 30, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.