Rmu_sc0001009.1_g000027

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001009.1
OG0007552
Physical Location & Seq
Reverse (-)
140579 .. 143716
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
atgtcgtcgctagccaagccttctctcaccgcctcctccactcctaggtccgcgtaccccaacttccttgctaaccagcgcgcatttgcactcaccaccccattcctcgctgcaatgtcaaatccccgcaagctgttaccagagatgcagatccttgttgatgtggggtattctgtgagcaagcttttgaatctaccaacgcattatgcagctagtttgatattggttgcgtgttgcgcggtggttgttgaggatgatgatgaaactgatattgtcagttgccgcagattaaaagtagagaatgtaaaagcaatatcccctatcttgttagagtttagcttccttaaaaatgttgttgaagttgatcttcctgtttatgaggcaattgtaatatttcaggctggtaactatagcagtgacgaactatttgacaagttggacacccataaattgaatgcacatttaaaggcactgatgtctggccttacagcggaagtattgcatgcagccgttgaaatggtttgtaatcatcagcgtgatatcgaagagcctcatagtgcacaaatgtctaggataatggagaagattggtgagctcttcctgagttcagattggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

205

Amino Acids

22.68

Weight (kDa)

5.2

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 85 - 167 2.5e-14 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 53, 81, 239
AciI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
AclWI GGATC 1 cut(s) 145
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 45, 490
AgsI TTSAA 4 cut(s) 190, 359, 454, 515
AluBI AGCT 5 cut(s) 133, 184, 212, 339, 595
AluI AGCT 5 cut(s) 133, 184, 212, 339, 595
Alw21I GWGCWC 2 cut(s) 562, 597
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 1 cut(s) 145
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 3 cut(s) 110, 209, 506
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspA2I CCTAGG 1 cut(s) 44
AspLEI GCGC 3 cut(s) 81, 83, 239
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 19, 85, 602
AsuNHI GCTAGC 1 cut(s) 10
AvaII GGWCC 1 cut(s) 48
AvrII CCTAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 597
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
Bbv12I GWGCWC 2 cut(s) 562, 597
BbvI GCAGC 3 cut(s) 97, 221, 518
BceAI ACGGC 1 cut(s) 494
BfaI CTAG 4 cut(s) 11, 45, 213, 570
BfmI CTRYAG 1 cut(s) 409
BisI GCNGC 4 cut(s) 111, 210, 283, 507
BlnI CCTAGG 1 cut(s) 44
BlsI GCNGC 4 cut(s) 112, 211, 284, 508
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmsI GCATC 1 cut(s) 135
BmtI GCTAGC 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 490
Bse3DI GCAATG 1 cut(s) 120
Bse8I GATNNNNATC 1 cut(s) 149
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 259
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 45, 490
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 593
BsePI GCGCGC 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 3 cut(s) 97, 221, 518
Bsh1236I CGCG 3 cut(s) 53, 81, 239
BshFI GGCC 1 cut(s) 483
BsiHKAI GWGCWC 2 cut(s) 562, 597
BslI CCNNNNNNNGG 2 cut(s) 45, 490
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 562, 597
Bsp143I GATC 2 cut(s) 150, 364
BspACI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 3 cut(s) 53, 81, 239
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 145
BspQI GCTCTTC 2 cut(s) 540, 602
BsrDI GCAATG 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 44
BssHII GCGCGC 1 cut(s) 79
BssMI GATC 2 cut(s) 150, 364
BssT1I CCWWGG 1 cut(s) 44
Bst6I CTCTTC 2 cut(s) 540, 602
BstC8I GCNNGC 5 cut(s) 12, 81, 131, 182, 504
BstDEI CTNAG 1 cut(s) 602
BstF5I GGATG 1 cut(s) 259
BstFNI CGCG 3 cut(s) 53, 81, 239
BstHHI GCGC 3 cut(s) 81, 83, 239
BstKTI GATC 2 cut(s) 153, 367
BstMBI GATC 2 cut(s) 150, 364
BstMWI GCNNNNNNNGC 2 cut(s) 16, 236
BstNSI RCATGY 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 53, 81, 239
BstV1I GCAGC 3 cut(s) 97, 221, 518
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 1 cut(s) 483
BtsCI GGATG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 421, 470
Cac8I GCNNGC 5 cut(s) 12, 81, 131, 182, 504
CfoI GCGC 3 cut(s) 81, 83, 239
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 55
CviAII CATG 1 cut(s) 503
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 602
DpnI GATC 2 cut(s) 152, 366
DpnII GATC 2 cut(s) 150, 364
DraI TTTAAA 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 540, 602
EarI CTCTTC 2 cut(s) 540, 602
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 595
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 1 cut(s) 506
FaiI YATR 6 cut(s) 207, 378, 411, 447, 504, 555
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 1 cut(s) 502
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 4 cut(s) 111, 210, 283, 507
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 4 cut(s) 111, 210, 283, 507
FspBI CTAG 4 cut(s) 11, 45, 213, 570
GlaI GCGC 3 cut(s) 80, 82, 238
GluI GCNGC 4 cut(s) 111, 210, 283, 507
HaeIII GGCC 1 cut(s) 483
HhaI GCGC 3 cut(s) 81, 83, 239
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 3 cut(s) 79, 81, 237
HinP1I GCGC 3 cut(s) 79, 81, 237
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 1 cut(s) 190
HphI GGTGA 3 cut(s) 19, 85, 602
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 1 cut(s) 610
Hpy188III TCNNGA 1 cut(s) 601
Hpy8I GTNNAC 1 cut(s) 560
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 30
HpyCH4V TGCA 8 cut(s) 89, 113, 148, 209, 458, 502, 506, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 236
HpyF3I CTNAG 1 cut(s) 602
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 3 cut(s) 79, 81, 237
Kzo9I GATC 2 cut(s) 150, 364
LguI GCTCTTC 2 cut(s) 540, 602
LpnPI CCDG 7 cut(s) 89, 153, 383, 384, 387, 465, 614
Lsp1109I GCAGC 3 cut(s) 97, 221, 518
LweI GCATC 1 cut(s) 135
MaeI CTAG 4 cut(s) 11, 45, 213, 570
MaeIII GTNAC 3 cut(s) 135, 404, 416
MalI GATC 2 cut(s) 152, 366
MboI GATC 2 cut(s) 150, 364
MboII GAAGA 4 cut(s) 359, 557, 589, 595
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 2 cut(s) 562, 597
MluCI AATT 2 cut(s) 384, 449
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 6 cut(s) 43, 46, 116, 244, 373, 561
MseI TTAA 3 cut(s) 290, 345, 464
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 491
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 460
MvnI CGCG 3 cut(s) 53, 81, 239
MwoI GCNNNNNNNGC 2 cut(s) 16, 236
NdeII GATC 2 cut(s) 150, 364
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 1 cut(s) 506
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 506
PaeI GCATGC 1 cut(s) 506
PauI GCGCGC 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 540, 602
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 4 cut(s) 112, 211, 284, 508
Psp124BI GAGCTC 1 cut(s) 597
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 150
PteI GCGCGC 1 cut(s) 79
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 534
SacI GAGCTC 1 cut(s) 597
SapI GCTCTTC 2 cut(s) 540, 602
SaqAI TTAA 3 cut(s) 290, 345, 464
SatI GCNGC 4 cut(s) 111, 210, 283, 507
Sau3AI GATC 2 cut(s) 150, 364
Sau96I GGNCC 1 cut(s) 48
SduI GDGCHC 2 cut(s) 562, 597
SetI ASST 6 cut(s) 50, 135, 186, 214, 341, 597
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 534
SphI GCATGC 1 cut(s) 506
Sse9I AATT 2 cut(s) 384, 449
SsiI CCGC 6 cut(s) 30, 51, 127, 239, 283, 491
SspI AATATT 1 cut(s) 393
SspMI CTAG 4 cut(s) 11, 45, 213, 570
SstI GAGCTC 1 cut(s) 597
StyI CCWWGG 1 cut(s) 44
TaqI TCGA 1 cut(s) 543
TasI AATT 2 cut(s) 384, 449
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 3 cut(s) 290, 345, 464
Tru9I TTAA 3 cut(s) 290, 345, 464
TscAI CASTG 2 cut(s) 421, 477
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 3 cut(s) 110, 209, 506
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 2 cut(s) 421, 477
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 48
XceI RCATGY 1 cut(s) 506
XmaJI CCTAGG 1 cut(s) 44
XspI CTAG 4 cut(s) 11, 45, 213, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.