Rmu_sc0001044.1_g000026

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001044.1
OG0006094
Physical Location & Seq
Reverse (-)
92311 .. 94188
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
atgcgcggcatgggtggcttaacgatgtggttctgggcgctgacgtcagcagcagctatattggaggcgtcagtgtcgtcgtcgtcgtcgtcgtcttgggtggctgacgggacggtgctggagctggacgattccaacttcgaatcggcgatttcagcgctcgacttggtcttggtggacttccacgccccatggtgcccccacagcaagagtctctctcctcagttggacgcggctgcgccgatgcttgcttcattgaagaaaccaatagtgatagcaaaagttgatgctgacaagtctacatctctagcttgcaaatacggagtcgatggatatcctacgctgaagctctttatacatggtgtcccccaagagtatatggggccaaggaaagccgatttgcttgttcgttatctcaagaaatttgcagctcccgatgttaccctacttgattcagactctgctattgctgattttgttggagcggcaggcacattcttccccatatatgttggttttggcttagatgagtccgtgatatcgaagttggcattgaagtacaaaaggaaggcctggttttcggttgcaaaggatttctctgaggatgtaatgctattgtatgattttgacaaggttcctgctctggtatcccttcatccaagttgcgatgagcgctacgtcttttacggcccctttgaagaggaattcttggaagatttcattaaacaaagtttgttacctttggccatgcccatcaaccatgacacattaaaatcattggacgatgatgaaaggaaaattatgttgacaattgtggaggatgaggatgaagaaagtcaaagaacttga

Protein Analysis

282

Amino Acids

31.17

Weight (kDa)

4.62

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 39 - 141 1.7e-22 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 195
AccBSI CCGCTC 1 cut(s) 485
AccI GTMKAC 1 cut(s) 299
AccII CGCG 2 cut(s) 6, 233
AciI CCGC 3 cut(s) 6, 233, 485
AcoI YGGCCR 1 cut(s) 744
AcsI RAATTY 2 cut(s) 422, 704
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 2 cut(s) 44, 68
AfaI GTAC 1 cut(s) 560
AfeI AGCGCT 2 cut(s) 159, 674
AgsI TTSAA 3 cut(s) 259, 556, 698
AjnI CCWGG 1 cut(s) 572
AluBI AGCT 5 cut(s) 56, 124, 311, 349, 431
AluI AGCT 5 cut(s) 56, 124, 311, 349, 431
Alw26I GTCTC 1 cut(s) 218
AlwNI CAGNNNCTG 1 cut(s) 461
Aor51HI AGCGCT 2 cut(s) 159, 674
AoxI GGCC 4 cut(s) 383, 570, 688, 744
ApeKI GCWGC 4 cut(s) 50, 53, 236, 428
ApoI RAATTY 2 cut(s) 422, 704
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
AspLEI GCGC 5 cut(s) 6, 40, 160, 241, 675
AspS9I GGNCC 2 cut(s) 383, 689
AsuII TTCGAA 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 200
BalI TGGCCA 1 cut(s) 746
BanI GGYRCC 1 cut(s) 195
BbvI GCAGC 4 cut(s) 62, 65, 223, 440
BccI CCATC 2 cut(s) 323, 761
BceAI ACGGC 1 cut(s) 703
BciT130I CCWGG 1 cut(s) 574
BciVI GTATCC 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 3 cut(s) 41, 161, 676
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 7 cut(s) 7, 51, 54, 234, 237, 429, 486
BlsI GCNGC 7 cut(s) 8, 52, 55, 235, 238, 430, 487
Bme1390I CCNGG 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 383, 689
BmiI GGNNCC 4 cut(s) 197, 384, 636, 691
BmrFI CCNGG 1 cut(s) 574
BmsI GCATC 2 cut(s) 234, 277
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpmI CTGGAG 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 2 cut(s) 44, 68
BsaJI CCNNGG 2 cut(s) 191, 386
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 2 cut(s) 191, 386
BseGI GGATG 4 cut(s) 610, 655, 826, 832
BseJI GATNNNNATC 1 cut(s) 333
BseMII CTCAG 2 cut(s) 236, 591
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 4 cut(s) 62, 65, 223, 440
Bsh1236I CGCG 2 cut(s) 6, 233
BshFI GGCC 4 cut(s) 385, 572, 690, 746
BshNI GGYRCC 1 cut(s) 195
BslFI GGGAC 2 cut(s) 124, 350
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 124, 350
BsnI GGCC 4 cut(s) 385, 572, 690, 746
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 200
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 3 cut(s) 6, 233, 485
BspANI GGCC 4 cut(s) 385, 572, 690, 746
BspCNI CTCAG 2 cut(s) 235, 592
BspFNI CGCG 2 cut(s) 6, 233
BspLI GGNNCC 4 cut(s) 197, 384, 636, 691
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 191, 386
BssNI GRCGYC 2 cut(s) 44, 68
BssT1I CCWWGG 2 cut(s) 191, 386
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 1 cut(s) 693
BstACI GRCGYC 2 cut(s) 44, 68
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 3 cut(s) 249, 313, 490
BstDEI CTNAG 3 cut(s) 222, 523, 600
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 4 cut(s) 610, 655, 826, 832
BstFNI CGCG 2 cut(s) 6, 233
BstH2I RGCGCY 3 cut(s) 41, 161, 676
BstHHI GCGC 5 cut(s) 6, 40, 160, 241, 675
BstMAI GTCTC 1 cut(s) 218
BstMWI GCNNNNNNNGC 4 cut(s) 15, 155, 204, 672
BstNI CCWGG 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 572
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 2 cut(s) 6, 233
BstV1I GCAGC 4 cut(s) 62, 65, 223, 440
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 4 cut(s) 385, 572, 690, 746
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 681
BtsCI GGATG 4 cut(s) 610, 655, 826, 832
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 249, 313, 490
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 5 cut(s) 6, 40, 160, 241, 675
Cfr13I GGNCC 2 cut(s) 383, 689
CseI GACGC 2 cut(s) 57, 239
Csp6I GTAC 1 cut(s) 559
CviAII CATG 5 cut(s) 10, 192, 359, 748, 761
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 3 cut(s) 222, 523, 600
EaeI YGGCCR 1 cut(s) 744
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 2 cut(s) 191, 386
Eco147I AGGCCT 1 cut(s) 572
Eco32I GATATC 2 cut(s) 335, 540
Eco47III AGCGCT 2 cut(s) 159, 674
Eco57I CTGAAG 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 572
EcoRV GATATC 2 cut(s) 335, 540
EcoT14I CCWWGG 2 cut(s) 191, 386
ErhI CCWWGG 2 cut(s) 191, 386
FaeI CATG 5 cut(s) 13, 195, 362, 751, 764
FaqI GGGAC 2 cut(s) 124, 350
FatI CATG 5 cut(s) 9, 191, 358, 747, 760
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 7 cut(s) 7, 51, 54, 234, 237, 429, 486
FokI GGATG 4 cut(s) 617, 642, 833, 839
Fsp4HI GCNGC 7 cut(s) 7, 51, 54, 234, 237, 429, 486
FspBI CTAG 1 cut(s) 308
GlaI GCGC 5 cut(s) 5, 39, 159, 240, 674
GluI GCNGC 7 cut(s) 7, 51, 54, 234, 237, 429, 486
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 3 cut(s) 41, 161, 676
HaeIII GGCC 4 cut(s) 385, 572, 690, 746
HgaI GACGC 2 cut(s) 57, 239
HhaI GCGC 5 cut(s) 6, 40, 160, 241, 675
Hin1I GRCGYC 2 cut(s) 44, 68
Hin1II CATG 5 cut(s) 13, 195, 362, 751, 764
Hin6I GCGC 5 cut(s) 4, 38, 158, 239, 673
HinP1I GCGC 5 cut(s) 4, 38, 158, 239, 673
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HinfI GANTC 7 cut(s) 131, 143, 211, 324, 452, 458, 530
Hpy166II GTNNAC 3 cut(s) 178, 300, 807
Hpy188I TCNGA 2 cut(s) 457, 601
Hpy188III TCNNGA 2 cut(s) 418, 434
Hpy8I GTNNAC 3 cut(s) 178, 300, 807
Hpy99I CGWCG 5 cut(s) 82, 85, 88, 91, 94
HpyAV CCTTC 2 cut(s) 562, 662
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 44, 678
HpyCH4V TGCA 3 cut(s) 315, 428, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 155, 204, 672
HpyF3I CTNAG 3 cut(s) 222, 523, 600
HpySE526I ACGT 2 cut(s) 44, 678
Hsp92I GRCGYC 2 cut(s) 44, 68
Hsp92II CATG 5 cut(s) 13, 195, 362, 751, 764
HspAI GCGC 5 cut(s) 4, 38, 158, 239, 673
LmnI GCTCC 3 cut(s) 121, 436, 482
LpnPI CCDG 8 cut(s) 19, 104, 110, 474, 559, 586, 629, 651
Lsp1109I GCAGC 4 cut(s) 62, 65, 223, 440
LweI GCATC 2 cut(s) 234, 277
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 44, 678
MaeIII GTNAC 2 cut(s) 439, 735
MbiI CCGCTC 1 cut(s) 485
MboII GAAGA 5 cut(s) 271, 490, 710, 725, 842
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 4 cut(s) 422, 704, 798, 810
MluNI TGGCCA 1 cut(s) 746
MlyI GAGTC 4 cut(s) 220, 333, 452, 539
MmeI TCCRAC 3 cut(s) 159, 207, 460
MnlI CCTC 6 cut(s) 58, 231, 595, 694, 811, 817
Mox20I TGGCCA 1 cut(s) 746
MscI TGGCCA 1 cut(s) 746
MseI TTAA 3 cut(s) 20, 723, 770
Msp20I TGGCCA 1 cut(s) 746
MspR9I CCNGG 1 cut(s) 574
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 2 cut(s) 6, 233
MwoI GCNNNNNNNGC 4 cut(s) 15, 155, 204, 672
NcoI CCATGG 1 cut(s) 191
NlaIII CATG 5 cut(s) 13, 195, 362, 751, 764
NlaIV GGNNCC 4 cut(s) 197, 384, 636, 691
NspV TTCGAA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 572
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 86, 89
PfeI GAWTC 3 cut(s) 131, 143, 452
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 7 cut(s) 8, 52, 55, 235, 238, 430, 487
PleI GAGTC 4 cut(s) 219, 332, 452, 538
PpsI GAGTC 4 cut(s) 219, 332, 452, 538
Psp6I CCWGG 1 cut(s) 572
PspGI CCWGG 1 cut(s) 572
PspN4I GGNNCC 4 cut(s) 197, 384, 636, 691
PspPI GGNCC 2 cut(s) 383, 689
PstNI CAGNNNCTG 1 cut(s) 461
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SaqAI TTAA 3 cut(s) 20, 723, 770
SatI GCNGC 7 cut(s) 7, 51, 54, 234, 237, 429, 486
Sau96I GGNCC 2 cut(s) 383, 689
SchI GAGTC 4 cut(s) 220, 333, 452, 539
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 1 cut(s) 200
SetI ASST 9 cut(s) 47, 58, 126, 313, 351, 433, 636, 681, 742
SfaNI GCATC 2 cut(s) 234, 277
SfuI TTCGAA 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 4 cut(s) 422, 704, 798, 810
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 3 cut(s) 6, 233, 485
SspMI CTAG 1 cut(s) 308
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 1 cut(s) 572
StyI CCWWGG 2 cut(s) 191, 386
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 47, 681
TaqI TCGA 4 cut(s) 141, 162, 327, 542
TasI AATT 4 cut(s) 422, 704, 798, 810
TatI WGTACW 1 cut(s) 558
TauI GCSGC 3 cut(s) 9, 236, 488
TfiI GAWTC 3 cut(s) 131, 143, 452
Tru1I TTAA 3 cut(s) 20, 723, 770
Tru9I TTAA 3 cut(s) 20, 723, 770
TscAI CASTG 1 cut(s) 78
TseI GCWGC 4 cut(s) 50, 53, 236, 428
TspDTI ATGAA 5 cut(s) 243, 644, 709, 804, 843
TspGWI ACGGA 2 cut(s) 336, 523
TspRI CASTG 1 cut(s) 78
Tth111I GACNNNGTC 1 cut(s) 167
XapI RAATTY 2 cut(s) 422, 704
XmiI GTMKAC 1 cut(s) 299
XspI CTAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.