Rmu_sc0002245.1_g000034

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002245.1
OG0006472
Physical Location & Seq
Forward (+)
150326 .. 150835
510 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
atgctcctcttcttcctctccaccgccgcaagcccctctctttctctctccgactcctcttccgcctcggtcttcgagctcttacccgaatatgggcttcccagtgggatcttacccgccttggtttccaactacacgttgtctgccgacgacggccgattcgtcgtcgttttggagaaggcgtgctatattcagttcgagtacttggtgaactacgagacccggatcaccgggaagctgagctacggcgcgatcacggagctgaagggcattcaggtccaaagactgttcttttggttcgacgtcgatgagatcaggatcgatttgccgccgtccgatagtatttacttcaccgttgggagcatcaacaagaagctcgatgtcgatcagttccagagcgttctttcttgccgcgacgggctttcgggttcgggttcgggttcggtttcttgccttgggtcgttgaaacgggccattcaggtagtcactgctttattgattaaagtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

169

Amino Acids

18.41

Weight (kDa)

4.76

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 26 - 132 1.2e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 306
AccII CGCG 2 cut(s) 251, 414
AciI CCGC 6 cut(s) 24, 27, 63, 117, 329, 412
AclWI GGATC 3 cut(s) 116, 233, 326
AcoI YGGCCR 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 92, 93
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 1 cut(s) 466
AluBI AGCT 5 cut(s) 79, 238, 243, 262, 376
AluI AGCT 5 cut(s) 79, 238, 243, 262, 376
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 3 cut(s) 116, 233, 326
AoxI GGCC 2 cut(s) 154, 471
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 276, 308
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 277, 471
AsuC2I CCSGG 2 cut(s) 223, 232
AsuHPI GGTGA 3 cut(s) 220, 220, 343
AvaII GGWCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 81
BceAI ACGGC 3 cut(s) 169, 262, 316
BcnI CCSGG 2 cut(s) 223, 232
BcoDI GTCTC 1 cut(s) 212
BisI GCNGC 3 cut(s) 27, 329, 412
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 3 cut(s) 28, 330, 413
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 2 cut(s) 223, 232
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 277, 471
BmrFI CCNGG 2 cut(s) 223, 232
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 1 cut(s) 372
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 64
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 223, 232
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 2 cut(s) 317, 384
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 66, 120, 454
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 93
Bse1I ACTGG 1 cut(s) 102
Bse8I GATNNNNATC 2 cut(s) 317, 384
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 3 cut(s) 66, 120, 454
BseJI GATNNNNATC 2 cut(s) 317, 384
BseLI CCNNNNNNNGG 2 cut(s) 92, 93
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 46
BseX3I CGGCCG 1 cut(s) 154
Bsh1236I CGCG 2 cut(s) 251, 414
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 156, 473
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 2 cut(s) 223, 231
BslI CCNNNNNNNGG 2 cut(s) 92, 93
BsmAI GTCTC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 2 cut(s) 156, 473
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 6 cut(s) 108, 225, 252, 312, 318, 385
Bsp1720I GCTNAGC 1 cut(s) 239
BspACI CCGC 6 cut(s) 24, 27, 63, 117, 329, 412
BspANI GGCC 2 cut(s) 156, 473
BspCNI CTCAG 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 321
BspFNI CGCG 2 cut(s) 251, 414
BspPI GGATC 3 cut(s) 116, 233, 326
BspTNI GGTCTC 1 cut(s) 212
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 3 cut(s) 66, 120, 454
BssMI GATC 6 cut(s) 108, 225, 252, 312, 318, 385
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 120, 454
Bst4CI ACNGT 2 cut(s) 288, 355
Bst6I CTCTTC 2 cut(s) 14, 64
BstACI GRCGYC 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 31, 184
BstDEI CTNAG 1 cut(s) 239
BstFNI CGCG 2 cut(s) 251, 414
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 6 cut(s) 111, 228, 255, 315, 321, 388
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 6 cut(s) 108, 225, 252, 312, 318, 385
BstMCI CGRYCG 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 221, 230
BstUI CGCG 2 cut(s) 251, 414
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BstZI CGGCCG 1 cut(s) 154
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 2 cut(s) 156, 473
BsuTUI ATCGAT 1 cut(s) 321
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 109, 486
Cac8I GCNNGC 2 cut(s) 31, 184
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 277, 471
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 202
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 239
DpnI GATC 6 cut(s) 110, 227, 254, 314, 320, 387
DpnII GATC 6 cut(s) 108, 225, 252, 312, 318, 385
EaeI YGGCCR 1 cut(s) 154
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 2 cut(s) 14, 64
EarI CTCTTC 2 cut(s) 14, 64
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 120, 454
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 277
Eco52I CGGCCG 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 120, 454
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 120, 454
FaiI YATR 2 cut(s) 93, 189
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 3 cut(s) 27, 329, 412
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 27, 329, 412
GlaI GCGC 1 cut(s) 250
GluI GCNGC 3 cut(s) 27, 329, 412
HaeIII GGCC 2 cut(s) 156, 473
HapII CCGG 2 cut(s) 223, 231
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 2 cut(s) 53, 159
HpaII CCGG 2 cut(s) 223, 231
HphI GGTGA 3 cut(s) 220, 220, 343
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 52, 337
Hpy188III TCNNGA 2 cut(s) 316, 394
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 7 cut(s) 152, 155, 167, 170, 305, 308, 419
HpyAV CCTTC 2 cut(s) 172, 259
HpyCH4III ACNGT 2 cut(s) 288, 355
HpyCH4IV ACGT 2 cut(s) 137, 303
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 2 cut(s) 137, 303
Hsp92I GRCGYC 1 cut(s) 303
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 6 cut(s) 108, 225, 252, 312, 318, 385
LmnI GCTCC 3 cut(s) 9, 259, 360
LpnPI CCDG 7 cut(s) 115, 236, 244, 260, 301, 407, 464
LweI GCATC 1 cut(s) 372
MaeII ACGT 2 cut(s) 137, 303
MaeIII GTNAC 1 cut(s) 484
MalI GATC 6 cut(s) 110, 227, 254, 314, 320, 387
MboI GATC 6 cut(s) 108, 225, 252, 312, 318, 385
MboII GAAGA 3 cut(s) 4, 51, 64
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 81
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 75, 153
MnlI CCTC 5 cut(s) 17, 26, 46, 67, 76
MseI TTAA 1 cut(s) 501
MspI CCGG 2 cut(s) 223, 231
MspR9I CCNGG 2 cut(s) 223, 232
Mva1269I GAATGC 1 cut(s) 270
MvnI CGCG 2 cut(s) 251, 414
NciI CCSGG 2 cut(s) 223, 232
NdeII GATC 6 cut(s) 108, 225, 252, 312, 318, 385
NmuCI GTSAC 1 cut(s) 484
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 3 cut(s) 28, 330, 413
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 81
PspPI GGNCC 2 cut(s) 277, 471
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 3 cut(s) 27, 329, 412
Sau3AI GATC 6 cut(s) 108, 225, 252, 312, 318, 385
Sau96I GGNCC 2 cut(s) 277, 471
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 223, 232
SduI GDGCHC 1 cut(s) 81
SetI ASST 9 cut(s) 81, 140, 240, 245, 264, 279, 306, 378, 483
SfaNI GCATC 1 cut(s) 372
SinI GGWCC 1 cut(s) 277
SsiI CCGC 6 cut(s) 24, 27, 63, 117, 329, 412
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 221, 230
StyI CCWWGG 2 cut(s) 120, 454
TaaI ACNGT 2 cut(s) 288, 355
TaiI ACGT 2 cut(s) 140, 306
TaqI TCGA 7 cut(s) 75, 198, 300, 306, 321, 378, 384
TaqII GACCGA 1 cut(s) 58
TatI WGTACW 1 cut(s) 201
TauI GCSGC 3 cut(s) 29, 331, 414
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 109, 493
TseFI GTSAC 1 cut(s) 484
Tsp45I GTSAC 1 cut(s) 484
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 2 cut(s) 109, 493
VpaK11BI GGWCC 1 cut(s) 277
ZraI GACGTC 1 cut(s) 304
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.