Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0003511.1

Orthogroup ID

OG0005778

Physical Location & Seq

Genomic Coordinates Reverse (-)

199744 .. 203820

Gene Structure 4077 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1224 bp

atgaagtgtggcgatgatgacgtggcggaggtggttgctcgctcgctattgcggtgttatttgcttggttttgggggggttttggatatggatatggatcctaaggagctcaatttgaacaaaggacctgaaggttgcggcggcggcggcggtggtgaggggtttatagataggagcaaggtgaggattttgctctgcgataacgacgagtccagctccgaagaagttctcaggcttcttttgaaatgctcttatcaagtcacttcggtcaagtctcctcggcaggtgatcgatgcattgaatgcagagggacctgatattgatatcatacttgccgaagtcgaccttccaatgagaaaaggaaccagaatgttgaagtacatcacacggcatagagagttgaggcgcattcctgtgatcatgatgtcagcacaggatgaggtctctactgttgtcaagtgcctgaggcttggagcggcagactatcttgtaaagcctttacgaactaatgagcttttaaacttgtggacacacatgtggagaagaagacacatgcttggacttgcagaaaagaacgtgtttagttatgactttgatacagttgtatcagatcctagtgatcctactacaaactcattctcggatgatacagaagagaagtctcagaagagtgccaatccagagatacctgtttctgctgccacggagcctccactggatgatcaattagagtttcagagtgatgttccagaaataagtgaccgccaaactggaaagttttcatctggcccaaaaaagagcgaactaaagattggggggtcatctgccttctttacctatgtcaaatcaagcacaattgaaaacaactcccaagtccatacaaaggataatggtgctgaacggtcaagagtggaagggaaacatccagcatgtagtcaacaaatggttgatgagtctctccaagtagatggaaatggagaagcatgggaaagctattcacaagaagatgactttccaaccagtaatagtatccctgattctctttcattggagaggtcttgcaccccacctggttcaatggaacttcagcatcagaagaatcatacggaagacagatctgtgaggaaggtaaatggtgtagatgttgatctcaatgggcaacctgcttctcttgatgatgacgaggatgatgaggatgagaaggatgatgaggagcttgcgtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009892 GO:0009987 GO:0010031 GO:0010467 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032501 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048511 GO:0048519 GO:0050789 GO:0050794 GO:0050879 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

CCT Response_reg

Protein Analysis

407

Amino Acids

45.13

Weight (kDa)

4.59

Isoelectric Point (pI)

48.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	63 - 174	1.7e-18	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61380
fragaria_vesca	FvH4_5g14560
malus_domestica	MD06G1197300.v1.1 MD14G1204700.v1.1
prunus_persica	Prupe.5G197900_v2.0.a1 Prupe.5G198000_v2.0.a1
pyrus_communis	pycom06g17420 pycom06g17430 pycom14g16910
rosa_chinensis	RchiOBHm_Chr7g0182001
rosa_laevigata	RLG00000005156 RLG00000005161
rosa_multiflora	Rmu_co8226389.1_g000001 Rmu_sc0003511.1_g000044 Rmu_sc0017796.1_g000001 Rmu_ssc0000297.1_g000003
rosa_roxburghii	Rroxscaffold_3G00271630
rosa_rugosa	Rorug06G0445000 Rorug06G0445100 Rorug06G0445300
rosa_samantha	Rh7AG047400 Rh7BG047400 Rh7BG047500 Rh7CG047700 Rh7DG046700
rosa_wichuraiana	Rw0G000460 Rw0G019690 Rw7G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	274
Acc36I	ACCTGC	2 cut(s)	274, 1171
AccBSI	CCGCTC	1 cut(s)	476
AccI	GTMKAC	1 cut(s)	342
AciI	CCGC	9 cut(s)	26, 52, 138, 141, 144, 147, 150, 476, 763
AclWI	GGATC	4 cut(s)	92, 105, 605, 614
AcuI	CTGAAG	2 cut(s)	150, 1070
AfaI	GTAC	1 cut(s)	380
AfiI	CCNNNNNNNGG	1 cut(s)	883
AflIII	ACRYGT	2 cut(s)	534, 576
AgsI	TTSAA	6 cut(s)	118, 244, 301, 376, 860, 1077
AjiI	CACGTC	1 cut(s)	22
AjnI	CCWGG	1 cut(s)	1069
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AleI	CACNNNNGTG	1 cut(s)	535
AluBI	AGCT	5 cut(s)	109, 216, 514, 993, 1216
AluI	AGCT	5 cut(s)	109, 216, 514, 993, 1216
Alw21I	GWGCWC	1 cut(s)	111
Alw26I	GTCTC	4 cut(s)	279, 448, 666, 960
AlwI	GGATC	4 cut(s)	92, 105, 605, 614
AoxI	GGCC	1 cut(s)	787
ApeKI	GCWGC	1 cut(s)	698
Asp700I	GAANNNNTTC	2 cut(s)	225, 778
AspLEI	GCGC	1 cut(s)	408
AspS9I	GGNCC	3 cut(s)	125, 311, 788
AsuHPI	GGTGA	3 cut(s)	167, 193, 298
AvaII	GGWCC	2 cut(s)	125, 311
AxyI	CCTNAGG	2 cut(s)	102, 464
BaeI	ACNNNNGTAYC	4 cut(s)	588, 588, 621, 621
BamHI	GGATCC	1 cut(s)	97
BanII	GRGCYC	1 cut(s)	111
BbsI	GAAGAC	2 cut(s)	553, 1116
Bbv12I	GWGCWC	1 cut(s)	111
BbvI	GCAGC	1 cut(s)	685
BccI	CCATC	1 cut(s)	962
BceAI	ACGGC	1 cut(s)	404
BciT130I	CCWGG	1 cut(s)	1071
BciVI	GTATCC	1 cut(s)	1040
BclI	TGATCA	2 cut(s)	417, 721
BcoDI	GTCTC	4 cut(s)	279, 448, 666, 960
BfaI	CTAG	1 cut(s)	615
BfuAI	ACCTGC	2 cut(s)	274, 1171
BfuI	GTATCC	1 cut(s)	1040
BglII	AGATCT	1 cut(s)	1115
BisI	GCNGC	6 cut(s)	139, 142, 145, 148, 477, 699
BlsI	GCNGC	6 cut(s)	140, 143, 146, 149, 478, 700
Bme1390I	CCNGG	1 cut(s)	1071
Bme18I	GGWCC	2 cut(s)	125, 311
BmgBI	CACGTC	1 cut(s)	22
BmgT120I	GGNCC	3 cut(s)	125, 311, 788
BmiI	GGNNCC	4 cut(s)	99, 312, 364, 708
BmrFI	CCNGG	1 cut(s)	1071
BmsI	GCATC	2 cut(s)	283, 1099
BpiI	GAAGAC	2 cut(s)	553, 1116
BplI	GAGNNNNNCTC	2 cut(s)	200, 232
Bsa29I	ATCGAT	1 cut(s)	291
BsaBI	GATNNNNATC	2 cut(s)	96, 1146
BsaI	GGTCTC	1 cut(s)	448
BsaJI	CCNNGG	2 cut(s)	278, 702
BsaXI	ACNNNNNCTCC	3 cut(s)	694, 724, 1205
Bsc4I	CCNNNNNNNGG	1 cut(s)	883
Bse1I	ACTGG	3 cut(s)	720, 775, 1020
Bse21I	CCTNAGG	2 cut(s)	102, 464
Bse8I	GATNNNNATC	2 cut(s)	96, 1146
BseBI	CCWGG	1 cut(s)	1071
BseCI	ATCGAT	1 cut(s)	291
BseDI	CCNNGG	2 cut(s)	278, 702
BseGI	GGATG	7 cut(s)	442, 649, 724, 922, 1192, 1201, 1210
BseJI	GATNNNNATC	2 cut(s)	96, 1146
BseLI	CCNNNNNNNGG	1 cut(s)	883
BseMII	CTCAG	3 cut(s)	244, 455, 677
BseNI	ACTGG	3 cut(s)	720, 775, 1020
BseRI	GAGGAG	1 cut(s)	267
BseXI	GCAGC	1 cut(s)	685
BshFI	GGCC	1 cut(s)	789
BshVI	ATCGAT	1 cut(s)	291
BsiHKAI	GWGCWC	1 cut(s)	111
BslFI	GGGAC	1 cut(s)	324
BslI	CCNNNNNNNGG	1 cut(s)	883
BsmAI	GTCTC	4 cut(s)	279, 448, 666, 960
BsmFI	GGGAC	1 cut(s)	324
BsmI	GAATGC	2 cut(s)	307, 408
BsnI	GGCC	1 cut(s)	789
Bso31I	GGTCTC	1 cut(s)	448
Bsp1286I	GDGCHC	1 cut(s)	111
Bsp143I	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
BspACI	CCGC	9 cut(s)	26, 52, 138, 141, 144, 147, 150, 476, 763
BspANI	GGCC	1 cut(s)	789
BspCNI	CTCAG	3 cut(s)	243, 456, 676
BspDI	ATCGAT	1 cut(s)	291
BspHI	TCATGA	1 cut(s)	420
BspLI	GGNNCC	4 cut(s)	99, 312, 364, 708
BspMI	ACCTGC	2 cut(s)	274, 1171
BspPI	GGATC	4 cut(s)	92, 105, 605, 614
BspTNI	GGTCTC	1 cut(s)	448
BsrBI	CCGCTC	1 cut(s)	476
BsrI	ACTGG	3 cut(s)	720, 775, 1020
BssECI	CCNNGG	2 cut(s)	278, 702
BssMI	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
Bst2UI	CCWGG	1 cut(s)	1071
Bst4CI	ACNGT	3 cut(s)	451, 601, 903
Bst6I	CTCTTC	2 cut(s)	648, 662
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstC8I	GCNNGC	3 cut(s)	40, 44, 1218
BstDEI	CTNAG	4 cut(s)	102, 230, 464, 663
BstDSI	CCRYGG	1 cut(s)	702
BstF5I	GGATG	7 cut(s)	442, 649, 724, 922, 1192, 1201, 1210
BstHHI	GCGC	1 cut(s)	408
BstKTI	GATC	8 cut(s)	100, 291, 420, 613, 622, 724, 1118, 1150
BstMAI	GTCTC	4 cut(s)	279, 448, 666, 960
BstMBI	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
BstMWI	GCNNNNNNNGC	3 cut(s)	144, 147, 302
BstNI	CCWGG	1 cut(s)	1071
BstNSI	RCATGY	3 cut(s)	538, 556, 933
BstSCI	CCNGG	1 cut(s)	1069
BstV1I	GCAGC	1 cut(s)	685
BstV2I	GAAGAC	2 cut(s)	553, 1116
BstX2I	RGATCY	3 cut(s)	97, 610, 1115
BstXI	CCANNNNNNTGG	1 cut(s)	968
BstYI	RGATCY	3 cut(s)	97, 610, 1115
Bsu15I	ATCGAT	1 cut(s)	291
Bsu36I	CCTNAGG	2 cut(s)	102, 464
BsuI	GTATCC	1 cut(s)	1040
BsuRI	GGCC	1 cut(s)	789
BsuTUI	ATCGAT	1 cut(s)	291
BtgI	CCRYGG	1 cut(s)	702
BtgZI	GCGATG	1 cut(s)	27
BtrI	CACGTC	1 cut(s)	22
BtsCI	GGATG	7 cut(s)	442, 649, 724, 922, 1192, 1201, 1210
BtsIMutI	CAGTG	1 cut(s)	713
BveI	ACCTGC	2 cut(s)	274, 1171
Cac8I	GCNNGC	3 cut(s)	40, 44, 1218
CciI	TCATGA	1 cut(s)	420
CfoI	GCGC	1 cut(s)	408
Cfr13I	GGNCC	3 cut(s)	125, 311, 788
ClaI	ATCGAT	1 cut(s)	291
CsiI	ACCWGGT	1 cut(s)	1069
Csp6I	GTAC	1 cut(s)	379
CviAII	CATG	5 cut(s)	421, 535, 553, 930, 984
CviQI	GTAC	1 cut(s)	379
DdeI	CTNAG	4 cut(s)	102, 230, 464, 663
DpnI	GATC	8 cut(s)	99, 290, 419, 612, 621, 723, 1117, 1149
DpnII	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
DraI	TTTAAA	1 cut(s)	519
Eam1104I	CTCTTC	2 cut(s)	648, 662
EarI	CTCTTC	2 cut(s)	648, 662
EciI	GGCGGA	1 cut(s)	41
Ecl136II	GAGCTC	1 cut(s)	109
Eco24I	GRGCYC	1 cut(s)	111
Eco31I	GGTCTC	1 cut(s)	448
Eco32I	GATATC	1 cut(s)	325
Eco47I	GGWCC	2 cut(s)	125, 311
Eco53kI	GAGCTC	1 cut(s)	109
Eco57I	CTGAAG	2 cut(s)	150, 1070
Eco81I	CCTNAGG	2 cut(s)	102, 464
EcoICRI	GAGCTC	1 cut(s)	109
EcoO109I	RGGNCCY	2 cut(s)	125, 311
EcoRII	CCWGG	1 cut(s)	1069
EcoRV	GATATC	1 cut(s)	325
EcoT22I	ATGCAT	1 cut(s)	298
EcoT38I	GRGCYC	1 cut(s)	111
FaeI	CATG	5 cut(s)	424, 538, 556, 933, 987
FalI	AAGNNNNNCTT	2 cut(s)	330, 362
FaqI	GGGAC	1 cut(s)	324
FatI	CATG	5 cut(s)	420, 534, 552, 929, 983
FbaI	TGATCA	2 cut(s)	417, 721
FblI	GTMKAC	1 cut(s)	342
Fnu4HI	GCNGC	6 cut(s)	139, 142, 145, 148, 477, 699
FokI	GGATG	7 cut(s)	449, 656, 731, 909, 1199, 1208, 1217
FriOI	GRGCYC	1 cut(s)	111
Fsp4HI	GCNGC	6 cut(s)	139, 142, 145, 148, 477, 699
FspBI	CTAG	1 cut(s)	615
GlaI	GCGC	1 cut(s)	407
GluI	GCNGC	6 cut(s)	139, 142, 145, 148, 477, 699
HaeIII	GGCC	1 cut(s)	789
HhaI	GCGC	1 cut(s)	408
Hin1II	CATG	5 cut(s)	424, 538, 556, 933, 987
Hin6I	GCGC	1 cut(s)	406
HinP1I	GCGC	1 cut(s)	406
HincII	GTYRAC	2 cut(s)	343, 938
HindII	GTYRAC	2 cut(s)	343, 938
HinfI	GANTC	4 cut(s)	209, 953, 1037, 1099
HphI	GGTGA	3 cut(s)	167, 193, 298
Hpy166II	GTNNAC	3 cut(s)	343, 528, 938
Hpy188I	TCNGA	6 cut(s)	220, 610, 643, 666, 738, 1095
Hpy188III	TCNNGA	5 cut(s)	421, 680, 749, 906, 1172
Hpy8I	GTNNAC	3 cut(s)	343, 528, 938
Hpy99I	CGWCG	1 cut(s)	209
HpyAV	CCTTC	6 cut(s)	125, 356, 838, 908, 1120, 1195
HpyCH4III	ACNGT	3 cut(s)	451, 601, 903
HpyCH4IV	ACGT	2 cut(s)	21, 576
HpyCH4V	TGCA	4 cut(s)	296, 305, 566, 1062
HpyF10VI	GCNNNNNNNGC	3 cut(s)	144, 147, 302
HpyF3I	CTNAG	4 cut(s)	102, 230, 464, 663
HpySE526I	ACGT	2 cut(s)	21, 576
Hsp92II	CATG	5 cut(s)	424, 538, 556, 933, 987
HspAI	GCGC	1 cut(s)	406
Ksp22I	TGATCA	2 cut(s)	417, 721
Kzo9I	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
LmnI	GCTCC	6 cut(s)	106, 174, 221, 473, 706, 1213
Lsp1109I	GCAGC	1 cut(s)	685
LweI	GCATC	2 cut(s)	283, 1099
MabI	ACCWGGT	1 cut(s)	1069
MaeI	CTAG	1 cut(s)	615
MaeII	ACGT	2 cut(s)	21, 576
MaeIII	GTNAC	2 cut(s)	259, 758
MalI	GATC	8 cut(s)	99, 290, 419, 612, 621, 723, 1117, 1149
MbiI	CCGCTC	1 cut(s)	476
MboI	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
MboII	GAAGA	8 cut(s)	233, 555, 558, 665, 679, 1016, 1108, 1121
MfeI	CAATTG	1 cut(s)	855
MflI	RGATCY	3 cut(s)	97, 610, 1115
MhlI	GDGCHC	1 cut(s)	111
MluCI	AATT	3 cut(s)	112, 725, 855
MlyI	GAGTC	2 cut(s)	218, 962
MmeI	TCCRAC	1 cut(s)	1040
Mph1103I	ATGCAT	1 cut(s)	298
MroXI	GAANNNNTTC	2 cut(s)	225, 778
MseI	TTAA	1 cut(s)	518
MslI	CAYNNNNRTG	2 cut(s)	413, 535
MspR9I	CCNGG	1 cut(s)	1071
MunI	CAATTG	1 cut(s)	855
Mva1269I	GAATGC	2 cut(s)	307, 408
MvaI	CCWGG	1 cut(s)	1071
MwoI	GCNNNNNNNGC	3 cut(s)	144, 147, 302
NdeII	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
NlaIII	CATG	5 cut(s)	424, 538, 556, 933, 987
NlaIV	GGNNCC	4 cut(s)	99, 312, 364, 708
NmeAIII	GCCGAG	1 cut(s)	259
NmuCI	GTSAC	2 cut(s)	259, 758
NsiI	ATGCAT	1 cut(s)	298
NspI	RCATGY	3 cut(s)	538, 556, 933
OliI	CACNNNNGTG	1 cut(s)	535
PagI	TCATGA	1 cut(s)	420
PaqCI	CACCTGC	1 cut(s)	274
PciI	ACATGT	1 cut(s)	534
PctI	GAATGC	2 cut(s)	307, 408
PdmI	GAANNNNTTC	2 cut(s)	225, 778
PfeI	GAWTC	2 cut(s)	1037, 1099
PkrI	GCNGC	6 cut(s)	140, 143, 146, 149, 478, 700
PleI	GAGTC	2 cut(s)	217, 961
PpsI	GAGTC	2 cut(s)	217, 961
PpuMI	RGGWCCY	2 cut(s)	125, 311
PscI	ACATGT	1 cut(s)	534
Psp124BI	GAGCTC	1 cut(s)	111
Psp5II	RGGWCCY	2 cut(s)	125, 311
Psp6I	CCWGG	1 cut(s)	1069
PspGI	CCWGG	1 cut(s)	1069
PspN4I	GGNNCC	4 cut(s)	99, 312, 364, 708
PspPI	GGNCC	3 cut(s)	125, 311, 788
PspPPI	RGGWCCY	2 cut(s)	125, 311
PsuI	RGATCY	3 cut(s)	97, 610, 1115
RsaI	GTAC	1 cut(s)	380
RsaNI	GTAC	1 cut(s)	379
RseI	CAYNNNNRTG	2 cut(s)	413, 535
SacI	GAGCTC	1 cut(s)	111
SalI	GTCGAC	1 cut(s)	341
SaqAI	TTAA	1 cut(s)	518
SatI	GCNGC	6 cut(s)	139, 142, 145, 148, 477, 699
Sau3AI	GATC	8 cut(s)	97, 288, 417, 610, 619, 721, 1115, 1147
Sau96I	GGNCC	3 cut(s)	125, 311, 788
SchI	GAGTC	2 cut(s)	218, 962
ScrFI	CCNGG	1 cut(s)	1071
SduI	GDGCHC	1 cut(s)	111
SexAI	ACCWGGT	1 cut(s)	1069
SfaNI	GCATC	2 cut(s)	283, 1099
SinI	GGWCC	2 cut(s)	125, 311
SmiMI	CAYNNNNRTG	2 cut(s)	413, 535
Sse9I	AATT	3 cut(s)	112, 725, 855
SsiI	CCGC	9 cut(s)	26, 52, 138, 141, 144, 147, 150, 476, 763
SspMI	CTAG	1 cut(s)	615
SstI	GAGCTC	1 cut(s)	111
StyD4I	CCNGG	1 cut(s)	1069
TaaI	ACNGT	3 cut(s)	451, 601, 903
TaiI	ACGT	2 cut(s)	24, 579
TaqI	TCGA	2 cut(s)	291, 342
TaqII	GACCGA	1 cut(s)	256
TasI	AATT	3 cut(s)	112, 725, 855
TatI	WGTACW	1 cut(s)	378
TauI	GCSGC	5 cut(s)	141, 144, 147, 150, 479
TfiI	GAWTC	2 cut(s)	1037, 1099
Tru1I	TTAA	1 cut(s)	518
Tru9I	TTAA	1 cut(s)	518
TscAI	CASTG	1 cut(s)	720
TseFI	GTSAC	2 cut(s)	259, 758
TseI	GCWGC	1 cut(s)	698
Tsp45I	GTSAC	2 cut(s)	259, 758
TspDTI	ATGAA	3 cut(s)	17, 771, 1035
TspGWI	ACGGA	2 cut(s)	719, 1121
TspRI	CASTG	1 cut(s)	720
VpaK11BI	GGWCC	2 cut(s)	125, 311
XceI	RCATGY	3 cut(s)	538, 556, 933
XmiI	GTMKAC	1 cut(s)	342
XmnI	GAANNNNTTC	2 cut(s)	225, 778
XspI	CTAG	1 cut(s)	615
Zsp2I	ATGCAT	1 cut(s)	298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.