Rmu_sc0003749.1_g000001

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003749.1
OG0001724
Physical Location & Seq
Reverse (-)
8911 .. 13443
4533 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
atgtcgtcggacttgttgctaaagccgtcgtgcggcggatgcggatcgacttctgatttgtacggaagcaactgtaagcacatgacgctgtgctgtgcttgcggcaaaaccatggcggagaacaaagccaaatgctacgactgcggcgccgtcgtcactcgcttgattcgagaatataatgtgagggcaagcactgccagcgagaagagctacttcattggtaggtttgtgacggggttgcccaactttttgaagaagaagagtgctgaaaataaatggactcttcgcaaggatctttcggatccgagaaggagtaaggataaggatggacaagatcagaagaaatataaggatgttcgcccgcctactgaggcctctcgggacgaaactggccagtctcagtacaacggtcaactcgaaggtgcacagtcagcaatttactacctactgatgttgcaaggaaaggagttttctgctattcctgctggttcgtggttcaactttaacaaggttgcacaatataagcaacttactctagaggaagcagaagagaagatgaacaataggaaaaagacacaagatggatatgaaagatggatgatgaagtctggaactaatgaacctggtccacttcgtgagaaggaaactggtgtggctggtggtaagggacgcaaaaaaatgactggtgatgatgaggagggaaatttctctgataaaggggaggaagatgaggaagaagagacagcaaggaaaaatagacttggtctcaacaaaaaagttggtgatgacgaggatgatgaaggtggaaatgaagaagatgacttagatgatgatagcagtgagaagggtgatgattgggagcacgaagaaatattcaccgatgatgatgaagctgttggtattaatcctgaggagagagaagaattcgaacctgaggttccagctcccccagaaattaagcaggatgatgacgatgatgataataatgaagaggagggtgggctcagcaaatcaggaaaagagttgaagaagctacttgggcgaagtggtggtctgaatgattcagatgcagaggacgacgacgacgacgacgatgatatggatgatgatattgccttgcctcaagcgctggcaccaaagcagaaggatgcacccaaacaggaaccagttgacaacagtcctattaaacccgggacttctggatctgctgctaaagcaaacccatcttcctccaagtcggcaaagggaaagagaaaattaaatggtgatgacgctaaagcatccaacagtgcacctcccaagaaggtgaagactgaaaatgagcaaaaatcagtcaaagaggagcctgcttctgaaagtagtgtgcctgcaaaaggtactgctgcatcctcgaaaacgggttcaacaccatctggtggtcctacaggtcctgtatctgaggaagaaattagggcagttttgatgcaaaagtcacccatcaccacacgcgatcttgttaataacttccccggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

504

Amino Acids

55.05

Weight (kDa)

4.68

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 48 - 502 1.4e-100 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 1132
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccII CGCG 1 cut(s) 1488
AciI CCGC 7 cut(s) 33, 36, 42, 102, 116, 144, 362
AclWI GGATC 5 cut(s) 52, 296, 300, 309, 1210
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 2 cut(s) 703, 923
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 3 cut(s) 62, 404, 1378
AfeI AGCGCT 1 cut(s) 1128
AfiI CCNNNNNNNGG 3 cut(s) 32, 1373, 1415
AgsI TTSAA 4 cut(s) 253, 499, 1027, 1404
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 4 cut(s) 210, 893, 944, 1033
AluI AGCT 4 cut(s) 210, 893, 944, 1033
Alw21I GWGCWC 3 cut(s) 427, 864, 1294
Alw26I GTCTC 3 cut(s) 402, 734, 770
Alw44I GTGCAC 2 cut(s) 423, 1290
AlwI GGATC 5 cut(s) 52, 296, 300, 309, 1210
AlwNI CAGNNNCTG 1 cut(s) 1430
Ama87I CYCGRG 2 cut(s) 378, 1190
Aor51HI AGCGCT 1 cut(s) 1128
AoxI GGCC 2 cut(s) 372, 391
ApaLI GTGCAC 2 cut(s) 423, 1290
ApeKI GCWGC 2 cut(s) 1208, 1382
ApoI RAATTY 2 cut(s) 703, 923
ArsI GACNNNNNNTTYG 4 cut(s) 40, 72, 1036, 1068
AseI ATTAAT 1 cut(s) 903
AspLEI GCGC 2 cut(s) 149, 1129
AspS9I GGNCC 3 cut(s) 626, 1418, 1427
AsuC2I CCSGG 3 cut(s) 1191, 1192, 1509
AsuHPI GGTGA 8 cut(s) 698, 794, 860, 868, 1277, 1318, 1464, 1471
AsuII TTCGAA 1 cut(s) 927
AvaI CYCGRG 2 cut(s) 378, 1190
AvaII GGWCC 3 cut(s) 626, 1418, 1427
AxyI CCTNAGG 2 cut(s) 909, 933
BaeGI GKGCMC 2 cut(s) 427, 1294
BalI TGGCCA 1 cut(s) 393
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 146, 1132
BanII GRGCYC 1 cut(s) 1005
BbsI GAAGAC 1 cut(s) 1316
Bbv12I GWGCWC 3 cut(s) 427, 864, 1294
BbvI GCAGC 2 cut(s) 1195, 1369
BccI CCATC 6 cut(s) 320, 575, 588, 1231, 1417, 1484
BceAI ACGGC 2 cut(s) 10, 134
BciT130I CCWGG 1 cut(s) 624
BcnI CCSGG 3 cut(s) 1191, 1192, 1509
BcoDI GTCTC 3 cut(s) 402, 734, 770
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 1422
BfoI RGCGCY 2 cut(s) 150, 1130
BisI GCNGC 5 cut(s) 34, 103, 145, 1209, 1383
BlpI GCTNAGC 1 cut(s) 1004
BlsI GCNGC 5 cut(s) 35, 104, 146, 1210, 1384
Bme1390I CCNGG 4 cut(s) 624, 1191, 1192, 1509
Bme18I GGWCC 3 cut(s) 626, 1418, 1427
BmeT110I CYCGRG 2 cut(s) 378, 1190
BmgT120I GGNCC 3 cut(s) 626, 1418, 1427
BmiI GGNNCC 6 cut(s) 148, 303, 939, 1134, 1164, 1344
BmrFI CCNGG 4 cut(s) 624, 1191, 1192, 1509
BmsI GCATC 6 cut(s) 29, 1057, 1138, 1289, 1394, 1452
BoxI GACNNNNGTC 1 cut(s) 1176
BpiI GAAGAC 1 cut(s) 1316
Bpu1102I GCTNAGC 1 cut(s) 1004
Bpu14I TTCGAA 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 1107
BpuMI CCSGG 3 cut(s) 1191, 1192, 1509
BsaBI GATNNNNATC 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 3 cut(s) 111, 1190, 1507
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 1373, 1415
Bse1I ACTGG 5 cut(s) 394, 394, 652, 688, 1166
Bse21I CCTNAGG 2 cut(s) 909, 933
Bse8I GATNNNNATC 1 cut(s) 43
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 3 cut(s) 111, 1190, 1507
BseJI GATNNNNATC 1 cut(s) 43
BseLI CCNNNNNNNGG 3 cut(s) 32, 1373, 1415
BseMII CTCAG 6 cut(s) 360, 413, 900, 924, 1018, 1428
BseNI ACTGG 5 cut(s) 394, 394, 652, 688, 1166
BseRI GAGGAG 4 cut(s) 710, 926, 1007, 1355
BseSI GKGCMC 2 cut(s) 427, 1294
BseXI GCAGC 2 cut(s) 1195, 1369
Bsh1236I CGCG 1 cut(s) 1488
BshFI GGCC 2 cut(s) 374, 393
BshNI GGYRCC 2 cut(s) 146, 1132
BsiHKAI GWGCWC 3 cut(s) 427, 864, 1294
BsiHKCI CYCGRG 2 cut(s) 378, 1190
BsiSI CCGG 2 cut(s) 1191, 1509
BslFI GGGAC 3 cut(s) 395, 681, 1207
BslI CCNNNNNNNGG 3 cut(s) 32, 1373, 1415
BsmAI GTCTC 3 cut(s) 402, 734, 770
BsmFI GGGAC 3 cut(s) 395, 681, 1207
BsnI GGCC 2 cut(s) 374, 393
Bso31I GGTCTC 1 cut(s) 770
BsoBI CYCGRG 2 cut(s) 378, 1190
Bsp119I TTCGAA 1 cut(s) 927
Bsp1286I GDGCHC 4 cut(s) 427, 864, 1005, 1294
Bsp143I GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
Bsp1720I GCTNAGC 1 cut(s) 1004
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 7 cut(s) 33, 36, 42, 102, 116, 144, 362
BspANI GGCC 2 cut(s) 374, 393
BspCNI CTCAG 6 cut(s) 361, 412, 901, 925, 1017, 1429
BspFNI CGCG 1 cut(s) 1488
BspLI GGNNCC 6 cut(s) 148, 303, 939, 1134, 1164, 1344
BspPI GGATC 5 cut(s) 52, 296, 300, 309, 1210
BspQI GCTCTTC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 927
BspT107I GGYRCC 2 cut(s) 146, 1132
BspTNI GGTCTC 1 cut(s) 770
BsrI ACTGG 5 cut(s) 394, 394, 652, 688, 1166
BssECI CCNNGG 3 cut(s) 111, 1190, 1507
BssMI GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 5 cut(s) 74, 410, 429, 1178, 1289
Bst6I CTCTTC 6 cut(s) 200, 254, 288, 543, 732, 984
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBI TTCGAA 1 cut(s) 927
BstC8I GCNNGC 7 cut(s) 100, 190, 199, 362, 1131, 1347, 1368
BstDEI CTNAG 7 cut(s) 369, 399, 823, 909, 933, 1004, 1437
BstDSI CCRYGG 1 cut(s) 111
BstENI CCTNNNNNAGG 1 cut(s) 1371
BstFNI CGCG 1 cut(s) 1488
BstH2I RGCGCY 2 cut(s) 150, 1130
BstHHI GCGC 2 cut(s) 149, 1129
BstKTI GATC 6 cut(s) 47, 295, 304, 337, 1205, 1492
BstMAI GTCTC 3 cut(s) 402, 734, 770
BstMBI GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
BstNI CCWGG 1 cut(s) 624
BstPAI GACNNNNGTC 1 cut(s) 1176
BstSCI CCNGG 4 cut(s) 622, 1189, 1190, 1507
BstSFI CTRYAG 1 cut(s) 1422
BstSLI GKGCMC 2 cut(s) 427, 1294
BstUI CGCG 1 cut(s) 1488
BstV1I GCAGC 2 cut(s) 1195, 1369
BstV2I GAAGAC 1 cut(s) 1316
BstX2I RGATCY 3 cut(s) 292, 301, 1202
BstYI RGATCY 3 cut(s) 292, 301, 1202
Bsu36I CCTNAGG 2 cut(s) 909, 933
BsuRI GGCC 2 cut(s) 374, 393
BtgI CCRYGG 1 cut(s) 111
BtsI GCAGTG 2 cut(s) 192, 844
BtsIMutI CAGTG 3 cut(s) 192, 844, 1294
Cac8I GCNNGC 7 cut(s) 100, 190, 199, 362, 1131, 1347, 1368
CaiI CAGNNNCTG 1 cut(s) 1430
CfoI GCGC 2 cut(s) 149, 1129
Cfr13I GGNCC 3 cut(s) 626, 1418, 1427
Cfr9I CCCGGG 1 cut(s) 1190
CseI GACGC 3 cut(s) 94, 678, 1280
CsiI ACCWGGT 1 cut(s) 622
Csp6I GTAC 3 cut(s) 61, 403, 1377
CviAII CATG 2 cut(s) 82, 112
CviQI GTAC 3 cut(s) 61, 403, 1377
DdeI CTNAG 7 cut(s) 369, 399, 823, 909, 933, 1004, 1437
DinI GGCGCC 1 cut(s) 148
DpnI GATC 6 cut(s) 46, 294, 303, 336, 1204, 1491
DpnII GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
DraIII CACNNNGTG 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 6 cut(s) 200, 254, 288, 543, 732, 984
EarI CTCTTC 6 cut(s) 200, 254, 288, 543, 732, 984
EciI GGCGGA 2 cut(s) 51, 131
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 1005
Eco31I GGTCTC 1 cut(s) 770
Eco47I GGWCC 3 cut(s) 626, 1418, 1427
Eco47III AGCGCT 1 cut(s) 1128
Eco81I CCTNAGG 2 cut(s) 909, 933
Eco88I CYCGRG 2 cut(s) 378, 1190
EcoNI CCTNNNNNAGG 1 cut(s) 1371
EcoO109I RGGNCCY 1 cut(s) 1427
EcoRI GAATTC 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 1005
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 85, 115
FaiI YATR 7 cut(s) 83, 113, 177, 348, 522, 588, 1100
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 395, 681, 1207
FatI CATG 2 cut(s) 81, 111
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 5 cut(s) 34, 103, 145, 1209, 1383
FriOI GRGCYC 1 cut(s) 1005
Fsp4HI GCNGC 5 cut(s) 34, 103, 145, 1209, 1383
FspBI CTAG 1 cut(s) 536
GlaI GCGC 2 cut(s) 148, 1128
GluI GCNGC 5 cut(s) 34, 103, 145, 1209, 1383
HaeII RGCGCY 2 cut(s) 150, 1130
HaeIII GGCC 2 cut(s) 374, 393
HapII CCGG 2 cut(s) 1191, 1509
HgaI GACGC 3 cut(s) 94, 678, 1280
HhaI GCGC 2 cut(s) 149, 1129
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 85, 115
Hin6I GCGC 2 cut(s) 147, 1127
HinP1I GCGC 2 cut(s) 147, 1127
HincII GTYRAC 2 cut(s) 413, 1171
HindII GTYRAC 2 cut(s) 413, 1171
HinfI GANTC 3 cut(s) 166, 280, 1061
HpaII CCGG 2 cut(s) 1191, 1509
HphI GGTGA 8 cut(s) 698, 794, 860, 868, 1277, 1318, 1464, 1471
Hpy166II GTNNAC 5 cut(s) 413, 425, 629, 1171, 1292
Hpy188III TCNNGA 8 cut(s) 170, 380, 536, 609, 635, 908, 1014, 1200
Hpy8I GTNNAC 5 cut(s) 413, 425, 629, 1171, 1292
Hpy99I CGWCG 8 cut(s) 10, 31, 155, 1082, 1085, 1088, 1091, 1094
HpyAV CCTTC 7 cut(s) 303, 413, 634, 794, 838, 1138, 1297
HpyCH4III ACNGT 5 cut(s) 74, 410, 429, 1178, 1289
HpyCH4V TGCA 9 cut(s) 425, 457, 515, 1070, 1151, 1292, 1370, 1385, 1465
HpyF3I CTNAG 7 cut(s) 369, 399, 823, 909, 933, 1004, 1437
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 85, 115
HspAI GCGC 2 cut(s) 147, 1127
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 3 cut(s) 859, 949, 1342
Lsp1109I GCAGC 2 cut(s) 1195, 1369
LweI GCATC 6 cut(s) 29, 1057, 1138, 1289, 1394, 1452
MabI ACCWGGT 1 cut(s) 622
MaeI CTAG 1 cut(s) 536
MaeIII GTNAC 3 cut(s) 154, 229, 1470
MalI GATC 6 cut(s) 46, 294, 303, 336, 1204, 1491
MboI GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
MflI RGATCY 3 cut(s) 292, 301, 1202
MhlI GDGCHC 4 cut(s) 427, 864, 1005, 1294
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 6 cut(s) 435, 703, 923, 954, 1256, 1446
MluNI TGGCCA 1 cut(s) 393
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 274
MmeI TCCRAC 1 cut(s) 1308
Mox20I TGGCCA 1 cut(s) 393
MscI TGGCCA 1 cut(s) 393
MseI TTAA 6 cut(s) 504, 903, 957, 1185, 1259, 1497
Msp20I TGGCCA 1 cut(s) 393
MspI CCGG 2 cut(s) 1191, 1509
MspR9I CCNGG 4 cut(s) 624, 1191, 1192, 1509
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 1 cut(s) 1488
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 3 cut(s) 1191, 1192, 1509
NcoI CCATGG 1 cut(s) 111
NdeII GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
NlaIII CATG 2 cut(s) 85, 115
NlaIV GGNNCC 6 cut(s) 148, 303, 939, 1134, 1164, 1344
NmuCI GTSAC 3 cut(s) 154, 229, 1470
NspV TTCGAA 1 cut(s) 927
PceI AGGCCT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 5 cut(s) 166, 414, 1083, 1086, 1089
PfeI GAWTC 2 cut(s) 166, 1061
PflFI GACNNNGTC 1 cut(s) 762
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 5 cut(s) 35, 104, 146, 1210, 1384
PleI GAGTC 1 cut(s) 274
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 1427
PshAI GACNNNNGTC 1 cut(s) 1176
PshBI ATTAAT 1 cut(s) 903
Psp5II RGGWCCY 1 cut(s) 1427
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 6 cut(s) 148, 303, 939, 1134, 1164, 1344
PspPI GGNCC 3 cut(s) 626, 1418, 1427
PspPPI RGGWCCY 1 cut(s) 1427
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 3 cut(s) 292, 301, 1202
PsyI GACNNNGTC 1 cut(s) 762
RsaI GTAC 3 cut(s) 62, 404, 1378
RsaNI GTAC 3 cut(s) 61, 403, 1377
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 6 cut(s) 504, 903, 957, 1185, 1259, 1497
SatI GCNGC 5 cut(s) 34, 103, 145, 1209, 1383
Sau3AI GATC 6 cut(s) 44, 292, 301, 334, 1202, 1489
Sau96I GGNCC 3 cut(s) 626, 1418, 1427
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 4 cut(s) 624, 1191, 1192, 1509
SduI GDGCHC 4 cut(s) 427, 864, 1005, 1294
SexAI ACCWGGT 1 cut(s) 622
SfaNI GCATC 6 cut(s) 29, 1057, 1138, 1289, 1394, 1452
SfcI CTRYAG 1 cut(s) 1422
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 927
SinI GGWCC 3 cut(s) 626, 1418, 1427
SmaI CCCGGG 1 cut(s) 1192
SmlI CTYRAG 1 cut(s) 1122
SmoI CTYRAG 1 cut(s) 1122
Sse9I AATT 6 cut(s) 435, 703, 923, 954, 1256, 1446
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 7 cut(s) 33, 36, 42, 102, 116, 144, 362
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 873
SspMI CTAG 1 cut(s) 536
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 622, 1189, 1190, 1507
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 5 cut(s) 74, 410, 429, 1178, 1289
TaqI TCGA 5 cut(s) 47, 169, 417, 927, 1391
TasI AATT 6 cut(s) 435, 703, 923, 954, 1256, 1446
TatI WGTACW 1 cut(s) 402
TauI GCSGC 3 cut(s) 36, 105, 147
TfiI GAWTC 2 cut(s) 166, 1061
Tru1I TTAA 6 cut(s) 504, 903, 957, 1185, 1259, 1497
Tru9I TTAA 6 cut(s) 504, 903, 957, 1185, 1259, 1497
TscAI CASTG 3 cut(s) 199, 844, 1294
TseFI GTSAC 3 cut(s) 154, 229, 1470
TseI GCWGC 2 cut(s) 1208, 1382
Tsp45I GTSAC 3 cut(s) 154, 229, 1470
TspDTI ATGAA 9 cut(s) 205, 572, 603, 617, 633, 813, 825, 903, 1002
TspGWI ACGGA 1 cut(s) 78
TspMI CCCGGG 1 cut(s) 1190
TspRI CASTG 3 cut(s) 199, 844, 1294
Tth111I GACNNNGTC 1 cut(s) 762
Van91I CCANNNNNTGG 1 cut(s) 1415
VneI GTGCAC 2 cut(s) 423, 1290
VpaK11BI GGWCC 3 cut(s) 626, 1418, 1427
VspI ATTAAT 1 cut(s) 903
XagI CCTNNNNNAGG 1 cut(s) 1371
XapI RAATTY 2 cut(s) 703, 923
XbaI TCTAGA 1 cut(s) 535
XmaI CCCGGG 1 cut(s) 1190
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.