Rmu_sc0007038.1_g000004

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007038.1
OG0003254
Physical Location & Seq
Reverse (-)
13579 .. 18833
5255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1503 bp
atgatgcgattggaattgagacgaggagatgaaaagagtgatcttgagagtgattgcagtggttctggggaatgggaaggggagtttttccctgaaattcccaaaattaaatatgagggcccctctagcaagaacccctttgcgtacaaatggtataatgcagacgaggagattctggggaagaaaatgaaggattggttgaggtttagtgttgcattttggcacacattccgtggaacaggagctgacccattcggtgcgcctacaaaaaattggccttgggaggatggaactaactctgttgctatggccaagagaagaatgaaagccaactttgagttcataaataagcttggagtcgataggtggtgtttccatgacagggatattgctccggatggcaaaaccttggaggaaactaataaaaacttggatgaggtggtggcccttgccaaggagcttcagggaaccaaaattcgacctttgtggggcacagctcagctgtttatgcatcctcgctacatgcacggtggtgccactagccctgaactaggtgtatatgcatatgctgctgctcaagttaagaaagctattgaggtcacgcattatttaggtggtgaaaattatgtgttttggggtggccgtgagggttaccagagtctcctgaataccgacatgggacgagagcttgatcatctggcaaggtttcttgaagctgctgttgcttacaagaagaaaattggattcaatggaacactgttgattgaaccgaagcctcaagaacctactaaacaccagtatgactgggatgctgcaacaacagcaaatttcctgcgaaaatatggccttataggagaatttaaacttaacattgagtgcaaccatgccactctatctggtcacagctgtcatcatgagcttgaaactgcaagactcaatggtttactgggaaacattgatgcaaacactggagatcctcaaattggcaggttccaaaggaaagttgttacttttgtaccttgcagctgtcatcatgagcttgaaactgcaagactcaatggtttactgggaaacattgatgcaaatactggagatcctcaaattggatgggatacagatgaatttctcacagacattgcagaggcaactcgagttatgcttactgtggtaaaaaatggaggattggcaccaggaggcttcaactttgatgcaaaattacggagggaaagcacagatgtcgaggatttgttcattgctcatatcagtggaatggataccctggcccgtggactccgtaatgttgcaaagcttattgaggacggttctctggctgagcttgttcgcaaacgatatgagagttttgatacagaggttggggcccagatagaggctggtaagggtgattttgaatatcttgagaagaaggccatggaatggggtgaaccaaaagttccttctgccaagcaggaacttgcggagaggcatttccagtctgctttgtag

Protein Analysis

500

Amino Acids

56.25

Weight (kDa)

5.69

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 143 - 302 3.9e-06 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 978
AccB1I GGYRCC 2 cut(s) 533, 1186
AccB7I CCANNNNNTGG 1 cut(s) 1434
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 1 cut(s) 1475
AclWI GGATC 2 cut(s) 968, 1088
AcoI YGGCCR 2 cut(s) 309, 640
AcsI RAATTY 5 cut(s) 96, 474, 826, 857, 1121
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 2 cut(s) 146, 1017
AfiI CCNNNNNNNGG 8 cut(s) 382, 454, 488, 551, 983, 1103, 1387, 1434
AgsI TTSAA 7 cut(s) 713, 748, 767, 923, 1043, 1201, 1409
AjnI CCWGG 2 cut(s) 1189, 1278
AjuI GAANNNNNNNTTGG 4 cut(s) 323, 355, 1438, 1470
AleI CACNNNNGTG 1 cut(s) 531
Alw26I GTCTC 2 cut(s) 13, 665
AlwI GGATC 2 cut(s) 968, 1088
AlwNI CAGNNNCTG 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 1149
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 9 cut(s) 118, 275, 309, 444, 640, 844, 1281, 1377, 1425
ApaI GGGCCC 2 cut(s) 122, 1381
ApeKI GCWGC 5 cut(s) 569, 572, 716, 812, 1023
ApoI RAATTY 5 cut(s) 96, 474, 826, 857, 1121
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 6 cut(s) 118, 119, 445, 1282, 1377, 1378
AsuHPI GGTGA 3 cut(s) 629, 1412, 1451
AvaI CYCGRG 1 cut(s) 1149
BaeGI GKGCMC 3 cut(s) 122, 494, 1381
BaeI ACNNNNGTAYC 4 cut(s) 999, 1032, 1356, 1389
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 2 cut(s) 533, 1186
BanII GRGCYC 2 cut(s) 122, 1381
BbvI GCAGC 5 cut(s) 556, 559, 703, 799, 1035
BccI CCATC 3 cut(s) 281, 392, 1101
BceAI ACGGC 1 cut(s) 627
BcgI CGANNNNNNTGC 2 cut(s) 1219, 1253
BciT130I CCWGG 2 cut(s) 1191, 1280
BciVI GTATCC 2 cut(s) 1105, 1267
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 13, 665
BfaI CTAG 3 cut(s) 126, 540, 551
BfuAI ACCTGC 1 cut(s) 978
BfuI GTATCC 2 cut(s) 1105, 1267
BisI GCNGC 5 cut(s) 570, 573, 717, 813, 1024
BlpI GCTNAGC 2 cut(s) 498, 1332
BlsI GCNGC 5 cut(s) 571, 574, 718, 814, 1025
Bme1390I CCNGG 2 cut(s) 1191, 1280
BmeT110I CYCGRG 1 cut(s) 1149
BmgT120I GGNCC 6 cut(s) 118, 119, 445, 1282, 1377, 1378
BmiI GGNNCC 8 cut(s) 120, 121, 469, 535, 992, 1188, 1378, 1379
BmrFI CCNGG 2 cut(s) 1191, 1280
BmrI ACTGGG 3 cut(s) 814, 956, 1076
BmsI GCATC 5 cut(s) 520, 799, 949, 1069, 1198
BmuI ACTGGG 3 cut(s) 814, 956, 1076
BplI GAGNNNNNCTC 2 cut(s) 107, 139
BpmI CTGGAG 2 cut(s) 990, 1110
Bpu1102I GCTNAGC 2 cut(s) 498, 1332
BpuEI CTTGAG 4 cut(s) 65, 561, 762, 1436
BsaJI CCNNGG 7 cut(s) 232, 278, 408, 453, 1278, 1285, 1428
BsaWI WCCGGW 1 cut(s) 394
Bsc4I CCNNNNNNNGG 8 cut(s) 382, 454, 488, 551, 983, 1103, 1387, 1434
Bse1I ACTGG 7 cut(s) 796, 809, 951, 973, 1071, 1093, 1489
Bse3DI GCAATG 2 cut(s) 1134, 1251
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 2 cut(s) 1191, 1280
BseDI CCNNGG 7 cut(s) 232, 278, 408, 453, 1278, 1285, 1428
BseGI GGATG 6 cut(s) 292, 403, 439, 511, 814, 1112
BseLI CCNNNNNNNGG 8 cut(s) 382, 454, 488, 551, 983, 1103, 1387, 1434
BseMI GCAATG 2 cut(s) 1134, 1251
BseMII CTCAG 2 cut(s) 512, 1323
BseNI ACTGG 7 cut(s) 796, 809, 951, 973, 1071, 1093, 1489
BseRI GAGGAG 2 cut(s) 39, 182
BseSI GKGCMC 3 cut(s) 122, 494, 1381
BseXI GCAGC 5 cut(s) 556, 559, 703, 799, 1035
BshFI GGCC 9 cut(s) 120, 277, 311, 446, 642, 846, 1283, 1379, 1427
BshNI GGYRCC 2 cut(s) 533, 1186
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 8 cut(s) 382, 454, 488, 551, 983, 1103, 1387, 1434
BsmAI GTCTC 2 cut(s) 13, 665
BsmBI CGTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 693
BsnI GGCC 9 cut(s) 120, 277, 311, 446, 642, 846, 1283, 1379, 1427
BsoBI CYCGRG 1 cut(s) 1149
Bsp120I GGGCCC 2 cut(s) 118, 1377
Bsp1286I GDGCHC 3 cut(s) 122, 494, 1381
Bsp13I TCCGGA 1 cut(s) 394
Bsp143I GATC 4 cut(s) 40, 691, 973, 1093
Bsp1720I GCTNAGC 2 cut(s) 498, 1332
Bsp19I CCATGG 1 cut(s) 1428
BspACI CCGC 1 cut(s) 1475
BspANI GGCC 9 cut(s) 120, 277, 311, 446, 642, 846, 1283, 1379, 1427
BspCNI CTCAG 2 cut(s) 511, 1324
BspEI TCCGGA 1 cut(s) 394
BspHI TCATGA 2 cut(s) 913, 1033
BspLI GGNNCC 8 cut(s) 120, 121, 469, 535, 992, 1188, 1378, 1379
BspMI ACCTGC 1 cut(s) 978
BspPI GGATC 2 cut(s) 968, 1088
BspT107I GGYRCC 2 cut(s) 533, 1186
BsrDI GCAATG 2 cut(s) 1134, 1251
BsrI ACTGG 7 cut(s) 796, 809, 951, 973, 1071, 1093, 1489
BssECI CCNNGG 7 cut(s) 232, 278, 408, 453, 1278, 1285, 1428
BssMI GATC 4 cut(s) 40, 691, 973, 1093
BssT1I CCWWGG 4 cut(s) 278, 408, 453, 1428
Bst2UI CCWGG 2 cut(s) 1191, 1280
Bst4CI ACNGT 4 cut(s) 530, 759, 1165, 1322
BstAPI GCANNNNNTGC 1 cut(s) 569
BstDEI CTNAG 2 cut(s) 498, 1332
BstDSI CCRYGG 3 cut(s) 232, 1285, 1428
BstEII GGTNACC 1 cut(s) 650
BstENI CCTNNNNNAGG 2 cut(s) 452, 549
BstF5I GGATG 6 cut(s) 292, 403, 439, 511, 814, 1112
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 4 cut(s) 43, 694, 976, 1096
BstMAI GTCTC 2 cut(s) 13, 665
BstMBI GATC 4 cut(s) 40, 691, 973, 1093
BstMWI GCNNNNNNNGC 5 cut(s) 126, 508, 569, 722, 821
BstNI CCWGG 2 cut(s) 1191, 1280
BstNSI RCATGY 1 cut(s) 526
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 1189, 1278
BstSLI GKGCMC 3 cut(s) 122, 494, 1381
BstV1I GCAGC 5 cut(s) 556, 559, 703, 799, 1035
BstX2I RGATCY 2 cut(s) 973, 1093
BstYI RGATCY 2 cut(s) 973, 1093
BsuI GTATCC 2 cut(s) 1105, 1267
BsuRI GGCC 9 cut(s) 120, 277, 311, 446, 642, 846, 1283, 1379, 1427
BtgI CCRYGG 3 cut(s) 232, 1285, 1428
BtsCI GGATG 6 cut(s) 292, 403, 439, 511, 814, 1112
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 4 cut(s) 64, 755, 966, 1270
BveI ACCTGC 1 cut(s) 978
CaiI CAGNNNCTG 1 cut(s) 245
CciI TCATGA 2 cut(s) 913, 1033
CfoI GCGC 1 cut(s) 262
Cfr13I GGNCC 6 cut(s) 118, 119, 445, 1282, 1377, 1378
Csp6I GTAC 2 cut(s) 145, 1016
CviAII CATG 7 cut(s) 377, 523, 676, 884, 914, 1034, 1429
CviQI GTAC 2 cut(s) 145, 1016
DdeI CTNAG 2 cut(s) 498, 1332
DpnI GATC 4 cut(s) 42, 693, 975, 1095
DpnII GATC 4 cut(s) 40, 691, 973, 1093
DraI TTTAAA 1 cut(s) 862
EaeI YGGCCR 2 cut(s) 309, 640
Eco130I CCWWGG 4 cut(s) 278, 408, 453, 1428
Eco24I GRGCYC 2 cut(s) 122, 1381
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 1149
Eco91I GGTNACC 1 cut(s) 650
EcoNI CCTNNNNNAGG 2 cut(s) 452, 549
EcoO109I RGGNCCY 3 cut(s) 118, 119, 1377
EcoO65I GGTNACC 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 1189, 1278
EcoT14I CCWWGG 4 cut(s) 278, 408, 453, 1428
EcoT22I ATGCAT 2 cut(s) 513, 565
EcoT38I GRGCYC 2 cut(s) 122, 1381
ErhI CCWWGG 4 cut(s) 278, 408, 453, 1428
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 7 cut(s) 380, 526, 679, 887, 917, 1037, 1432
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 1 cut(s) 693
FatI CATG 7 cut(s) 376, 522, 675, 883, 913, 1033, 1428
FauNDI CATATG 1 cut(s) 565
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 5 cut(s) 570, 573, 717, 813, 1024
FokI GGATG 6 cut(s) 299, 410, 446, 498, 821, 1119
FriOI GRGCYC 2 cut(s) 122, 1381
Fsp4HI GCNGC 5 cut(s) 570, 573, 717, 813, 1024
FspBI CTAG 3 cut(s) 126, 540, 551
GlaI GCGC 1 cut(s) 261
GluI GCNGC 5 cut(s) 570, 573, 717, 813, 1024
GsuI CTGGAG 2 cut(s) 990, 1110
HaeIII GGCC 9 cut(s) 120, 277, 311, 446, 642, 846, 1283, 1379, 1427
HapII CCGG 1 cut(s) 395
HhaI GCGC 1 cut(s) 262
Hin1II CATG 7 cut(s) 380, 526, 679, 887, 917, 1037, 1432
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HindIII AAGCTT 2 cut(s) 350, 1307
HinfI GANTC 7 cut(s) 172, 357, 658, 744, 933, 1053, 1290
HpaII CCGG 1 cut(s) 395
HphI GGTGA 3 cut(s) 629, 1412, 1451
Hpy166II GTNNAC 4 cut(s) 944, 1064, 1289, 1442
Hpy188III TCNNGA 8 cut(s) 44, 395, 664, 710, 779, 914, 1034, 1415
Hpy8I GTNNAC 4 cut(s) 944, 1064, 1289, 1442
HpyAV CCTTC 4 cut(s) 71, 184, 1417, 1464
HpyCH4III ACNGT 4 cut(s) 530, 759, 1165, 1322
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 508, 569, 722, 821
HpyF3I CTNAG 2 cut(s) 498, 1332
Hsp92II CATG 7 cut(s) 380, 526, 679, 887, 917, 1037, 1432
HspAI GCGC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 394
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 4 cut(s) 40, 691, 973, 1093
LmnI GCTCC 3 cut(s) 242, 397, 457
Lsp1109I GCAGC 5 cut(s) 556, 559, 703, 799, 1035
LweI GCATC 5 cut(s) 520, 799, 949, 1069, 1198
MaeI CTAG 3 cut(s) 126, 540, 551
MaeIII GTNAC 4 cut(s) 598, 650, 899, 1006
MalI GATC 4 cut(s) 42, 693, 975, 1095
MboI GATC 4 cut(s) 40, 691, 973, 1093
MboII GAAGA 4 cut(s) 193, 330, 745, 1432
MflI RGATCY 2 cut(s) 973, 1093
MhlI GDGCHC 3 cut(s) 122, 494, 1381
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 5 cut(s) 366, 667, 927, 1047, 1284
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 513, 565
MroI TCCGGA 1 cut(s) 394
MscI TGGCCA 1 cut(s) 311
MseI TTAA 4 cut(s) 108, 582, 861, 867
MslI CAYNNNNRTG 3 cut(s) 531, 798, 1263
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 3 cut(s) 502, 906, 1026
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 1191, 1280
MvaI CCWGG 2 cut(s) 1191, 1280
MwoI GCNNNNNNNGC 5 cut(s) 126, 508, 569, 722, 821
NcoI CCATGG 1 cut(s) 1428
NdeI CATATG 1 cut(s) 565
NdeII GATC 4 cut(s) 40, 691, 973, 1093
NlaIII CATG 7 cut(s) 380, 526, 679, 887, 917, 1037, 1432
NlaIV GGNNCC 8 cut(s) 120, 121, 469, 535, 992, 1188, 1378, 1379
NmuCI GTSAC 2 cut(s) 598, 899
NsiI ATGCAT 2 cut(s) 513, 565
NspI RCATGY 1 cut(s) 526
OliI CACNNNNGTG 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 1149
PagI TCATGA 2 cut(s) 913, 1033
PfeI GAWTC 2 cut(s) 172, 744
PflMI CCANNNNNTGG 1 cut(s) 1434
PkrI GCNGC 5 cut(s) 571, 574, 718, 814, 1025
PleI GAGTC 5 cut(s) 365, 666, 927, 1047, 1284
PpsI GAGTC 5 cut(s) 365, 666, 927, 1047, 1284
Psp6I CCWGG 2 cut(s) 1189, 1278
PspEI GGTNACC 1 cut(s) 650
PspGI CCWGG 2 cut(s) 1189, 1278
PspN4I GGNNCC 8 cut(s) 120, 121, 469, 535, 992, 1188, 1378, 1379
PspOMI GGGCCC 2 cut(s) 118, 1377
PspPI GGNCC 6 cut(s) 118, 119, 445, 1282, 1377, 1378
PspXI VCTCGAGB 1 cut(s) 1149
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 2 cut(s) 973, 1093
PvuII CAGCTG 3 cut(s) 502, 906, 1026
RsaI GTAC 2 cut(s) 146, 1017
RsaNI GTAC 2 cut(s) 145, 1016
RseI CAYNNNNRTG 3 cut(s) 531, 798, 1263
SaqAI TTAA 4 cut(s) 108, 582, 861, 867
SatI GCNGC 5 cut(s) 570, 573, 717, 813, 1024
Sau3AI GATC 4 cut(s) 40, 691, 973, 1093
Sau96I GGNCC 6 cut(s) 118, 119, 445, 1282, 1377, 1378
SchI GAGTC 5 cut(s) 366, 667, 927, 1047, 1284
ScrFI CCNGG 2 cut(s) 1191, 1280
SduI GDGCHC 3 cut(s) 122, 494, 1381
SfaNI GCATC 5 cut(s) 520, 799, 949, 1069, 1198
Sfr274I CTCGAG 1 cut(s) 1149
SlaI CTCGAG 1 cut(s) 1149
SmiMI CAYNNNNRTG 3 cut(s) 531, 798, 1263
SmlI CTYRAG 5 cut(s) 44, 576, 777, 1149, 1415
SmoI CTYRAG 5 cut(s) 44, 576, 777, 1149, 1415
SsiI CCGC 1 cut(s) 1475
SspMI CTAG 3 cut(s) 126, 540, 551
StyD4I CCNGG 2 cut(s) 1189, 1278
StyI CCWWGG 4 cut(s) 278, 408, 453, 1428
TaaI ACNGT 4 cut(s) 530, 759, 1165, 1322
TaqI TCGA 4 cut(s) 360, 478, 1150, 1239
TfiI GAWTC 2 cut(s) 172, 744
Tru1I TTAA 4 cut(s) 108, 582, 861, 867
Tru9I TTAA 4 cut(s) 108, 582, 861, 867
TscAI CASTG 4 cut(s) 64, 762, 973, 1270
TseFI GTSAC 2 cut(s) 598, 899
TseI GCWGC 5 cut(s) 569, 572, 716, 812, 1023
Tsp45I GTSAC 2 cut(s) 598, 899
TspDTI ATGAA 6 cut(s) 45, 203, 331, 338, 1134, 1240
TspGWI ACGGA 3 cut(s) 221, 1234, 1283
TspRI CASTG 4 cut(s) 64, 762, 973, 1270
Van91I CCANNNNNTGG 1 cut(s) 1434
XagI CCTNNNNNAGG 2 cut(s) 452, 549
XapI RAATTY 5 cut(s) 96, 474, 826, 857, 1121
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 1388
XhoI CTCGAG 1 cut(s) 1149
XspI CTAG 3 cut(s) 126, 540, 551
Zsp2I ATGCAT 2 cut(s) 513, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.