Rroxscaffold_156G00440270

Ferric reduction oxidase 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000156
OG0001860
Physical Location & Seq
Forward (+)
4550 .. 8970
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_156G00440270.1

Sequence Viewer

Length: 981 bp
ATGGAGGCAGACAAGCTAACTGTTGTGATTAAGAGTCTAGGAAGTTGGTCTCAGAAACTTTACCAACAACTTTCTTCTAATTCTATGGATCATATTCAAGTTTCTGTTGAAGGGCCTTATGGACCTAATTCATCTCATTTTCTAAGGCACAAGGCTCTGGTGATGATTAGTGGAGGCAGTGGAATTACTCCGATGATCTCCATAATCCGAGAAATCATTTTCCAAAGCACCAAACCAGATTGCCATGTTCCGCCTGTTCGGTTGATATGTGCCTTCAAGAACTCAGCTGATTTCACCATGCTACAACTCTTGCTTCCAATTTCTGTCACACCTTCTAATTTTACCCAGCTGCAGTTTCAAATTGAAGCTTATGTGACCAGGGAGACACAGAAGCTACCTCCATCAGACTCCCTCAAGCTTCTCCAAACCCTATGGTTTAAGCCAGACCCACTGGACTCACCCATTTCTTTGGTTCTAGGACAAAACACTTGGCTATGGCTTGGTGCTATAATCTCATCCTCATTTGTCTTATTTCTCTTTGTTTTGGGCATTGTCAATCGTTTCTATATATACCCAATTGAACACAACACTGGTGAACACTACCATGTCTCCTTCATTTGCCTGTGGTACATGTTTTTGGTCTGTGCCTCCATTTTCATAGTCTCCAGTGCAGTCTTCATTTGCTGCAAGAGACAGAACGCCATTGAAGAAGGGAAGCGAACTCAGAATGCGAGATCAACACCAACAACTGATATCGAACTTGCGAGTTCTCCCCATAATCATAAGTCCCTGATCGCTCAAGCTACCCGAGTGCATTATGGAACTAGGCCTGACCTAAGGAAAATTTTGTTGGAGACCAAAGGATCTGATATTGGAGTGTTGGTTTGTGGCCCAAGGAAGATGAGGCATGAGGTTGCCACAGTTTGTTCATCCAGTTTGGCAGATAATCTGCACTTTGAGTCCATTAGCTTTAACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

326

Amino Acids

36.64

Weight (kDa)

8.58

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_6 PF08030 51 - 309 6.8e-22 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 96, 871
AcsI RAATTY 1 cut(s) 843
AfaI GTAC 1 cut(s) 629
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 7 cut(s) 98, 110, 277, 359, 365, 581, 707
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 8 cut(s) 16, 287, 349, 368, 394, 418, 803, 969
AluI AGCT 8 cut(s) 16, 287, 349, 368, 394, 418, 803, 969
Alw26I GTCTC 6 cut(s) 54, 377, 613, 667, 685, 848
AlwI GGATC 2 cut(s) 96, 871
Ama87I CYCGRG 1 cut(s) 807
AoxI GGCC 3 cut(s) 113, 827, 889
ApeKI GCWGC 2 cut(s) 349, 684
ApoI RAATTY 1 cut(s) 843
AspS9I GGNCC 3 cut(s) 113, 122, 890
AsuHPI GGTGA 4 cut(s) 172, 286, 450, 605
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 336, 671
BccI CCATC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 379
BcoDI GTCTC 6 cut(s) 54, 377, 613, 667, 685, 848
BfaI CTAG 3 cut(s) 38, 476, 825
BfmI CTRYAG 1 cut(s) 350
BisI GCNGC 2 cut(s) 350, 685
BlsI GCNGC 2 cut(s) 351, 686
Bme1390I CCNGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 807
BmgT120I GGNCC 3 cut(s) 113, 122, 890
BmrFI CCNGG 1 cut(s) 379
BpiI GAAGAC 1 cut(s) 667
BpmI CTGGAG 1 cut(s) 649
BpuEI CTTGAG 2 cut(s) 398, 783
BsaI GGTCTC 2 cut(s) 54, 848
BsaJI CCNNGG 2 cut(s) 378, 893
BsaXI ACNNNNNCTCC 4 cut(s) 593, 623, 867, 897
Bse1I ACTGG 5 cut(s) 456, 595, 666, 933, 980
Bse21I CCTNAGG 1 cut(s) 836
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 378, 893
BseGI GGATG 2 cut(s) 515, 929
BseMII CTCAG 3 cut(s) 65, 297, 737
BseNI ACTGG 5 cut(s) 456, 595, 666, 933, 980
BseXI GCAGC 2 cut(s) 336, 671
BseYI CCCAGC 1 cut(s) 345
BsgI GTGCAG 2 cut(s) 690, 935
BshFI GGCC 3 cut(s) 115, 829, 891
BsiHKCI CYCGRG 1 cut(s) 807
BslFI GGGAC 1 cut(s) 772
BsmAI GTCTC 6 cut(s) 54, 377, 613, 667, 685, 848
BsmFI GGGAC 1 cut(s) 772
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 3 cut(s) 115, 829, 891
Bso31I GGTCTC 2 cut(s) 54, 848
BsoBI CYCGRG 1 cut(s) 807
Bsp143I GATC 5 cut(s) 88, 195, 734, 792, 863
BspACI CCGC 1 cut(s) 251
BspANI GGCC 3 cut(s) 115, 829, 891
BspCNI CTCAG 3 cut(s) 64, 296, 736
BspMAI CTGCAG 1 cut(s) 354
BspPI GGATC 2 cut(s) 96, 871
BspTNI GGTCTC 2 cut(s) 54, 848
BsrI ACTGG 5 cut(s) 456, 595, 666, 933, 980
BssECI CCNNGG 2 cut(s) 378, 893
BssMI GATC 5 cut(s) 88, 195, 734, 792, 863
BssT1I CCWWGG 1 cut(s) 893
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 2 cut(s) 22, 922
BstDEI CTNAG 5 cut(s) 51, 143, 283, 723, 836
BstF5I GGATG 2 cut(s) 515, 929
BstKTI GATC 5 cut(s) 91, 198, 737, 795, 866
BstMAI GTCTC 6 cut(s) 54, 377, 613, 667, 685, 848
BstMBI GATC 5 cut(s) 88, 195, 734, 792, 863
BstNI CCWGG 1 cut(s) 379
BstNSI RCATGY 1 cut(s) 634
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 350
BstV1I GCAGC 2 cut(s) 336, 671
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 863
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 1 cut(s) 863
Bsu36I CCTNAGG 1 cut(s) 836
BsuRI GGCC 3 cut(s) 115, 829, 891
BtsCI GGATG 2 cut(s) 515, 929
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 4 cut(s) 184, 449, 588, 673
Cfr13I GGNCC 3 cut(s) 113, 122, 890
Csp6I GTAC 1 cut(s) 628
CviAII CATG 5 cut(s) 245, 298, 605, 631, 908
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 5 cut(s) 51, 143, 283, 723, 836
DpnI GATC 5 cut(s) 90, 197, 736, 794, 865
DpnII GATC 5 cut(s) 88, 195, 734, 792, 863
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 893
Eco147I AGGCCT 1 cut(s) 829
Eco31I GGTCTC 2 cut(s) 54, 848
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 122
Eco81I CCTNAGG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 807
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 377
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 1 cut(s) 893
ErhI CCWWGG 1 cut(s) 893
FaeI CATG 5 cut(s) 248, 301, 608, 634, 911
FaqI GGGAC 1 cut(s) 772
FatI CATG 5 cut(s) 244, 297, 604, 630, 907
Fnu4HI GCNGC 2 cut(s) 350, 685
FokI GGATG 2 cut(s) 502, 916
Fsp4HI GCNGC 2 cut(s) 350, 685
FspBI CTAG 3 cut(s) 38, 476, 825
GluI GCNGC 2 cut(s) 350, 685
GsaI CCCAGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 649
HaeIII GGCC 3 cut(s) 115, 829, 891
Hin1II CATG 5 cut(s) 248, 301, 608, 634, 911
HindIII AAGCTT 2 cut(s) 366, 416
HinfI GANTC 4 cut(s) 34, 407, 455, 959
HphI GGTGA 4 cut(s) 172, 286, 450, 605
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 6 cut(s) 54, 192, 209, 406, 726, 868
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 5 cut(s) 104, 283, 342, 622, 704
HpyCH4III ACNGT 2 cut(s) 22, 922
HpyCH4V TGCA 5 cut(s) 352, 671, 687, 814, 952
HpyF3I CTNAG 5 cut(s) 51, 143, 283, 723, 836
Hsp92II CATG 5 cut(s) 248, 301, 608, 634, 911
Kzo9I GATC 5 cut(s) 88, 195, 734, 792, 863
Lsp1109I GCAGC 2 cut(s) 336, 671
MaeI CTAG 3 cut(s) 38, 476, 825
MaeIII GTNAC 2 cut(s) 325, 373
MalI GATC 5 cut(s) 90, 197, 736, 794, 865
MboI GATC 5 cut(s) 88, 195, 734, 792, 863
MboII GAAGA 4 cut(s) 66, 667, 719, 910
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 1 cut(s) 863
MluCI AATT 8 cut(s) 79, 127, 183, 318, 337, 360, 576, 843
MlyI GAGTC 4 cut(s) 43, 401, 449, 968
MmeI TCCRAC 1 cut(s) 831
MnlI CCTC 7 cut(s) 167, 408, 422, 529, 658, 897, 904
MseI TTAA 3 cut(s) 30, 438, 972
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 2 cut(s) 287, 349
MspR9I CCNGG 1 cut(s) 379
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 1 cut(s) 379
NdeII GATC 5 cut(s) 88, 195, 734, 792, 863
NlaIII CATG 5 cut(s) 248, 301, 608, 634, 911
NmuCI GTSAC 2 cut(s) 325, 373
NspI RCATGY 1 cut(s) 634
PceI AGGCCT 1 cut(s) 829
PciI ACATGT 1 cut(s) 630
PctI GAATGC 1 cut(s) 733
PkrI GCNGC 2 cut(s) 351, 686
PleI GAGTC 4 cut(s) 42, 401, 449, 967
PpsI GAGTC 4 cut(s) 42, 401, 449, 967
PscI ACATGT 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 1 cut(s) 377
PspPI GGNCC 3 cut(s) 113, 122, 890
PstI CTGCAG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 863
PvuII CAGCTG 2 cut(s) 287, 349
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 3 cut(s) 30, 438, 972
SatI GCNGC 2 cut(s) 350, 685
Sau3AI GATC 5 cut(s) 88, 195, 734, 792, 863
Sau96I GGNCC 3 cut(s) 113, 122, 890
SchI GAGTC 4 cut(s) 43, 401, 449, 968
ScrFI CCNGG 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 350
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 413, 798
SmoI CTYRAG 2 cut(s) 413, 798
Sse9I AATT 8 cut(s) 79, 127, 183, 318, 337, 360, 576, 843
SseBI AGGCCT 1 cut(s) 829
SsiI CCGC 1 cut(s) 251
SspMI CTAG 3 cut(s) 38, 476, 825
StuI AGGCCT 1 cut(s) 829
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 2 cut(s) 22, 922
TaqI TCGA 1 cut(s) 756
TasI AATT 8 cut(s) 79, 127, 183, 318, 337, 360, 576, 843
Tru1I TTAA 3 cut(s) 30, 438, 972
Tru9I TTAA 3 cut(s) 30, 438, 972
TscAI CASTG 4 cut(s) 184, 456, 595, 673
TseFI GTSAC 2 cut(s) 325, 373
TseI GCWGC 2 cut(s) 349, 684
Tsp45I GTSAC 2 cut(s) 325, 373
TspDTI ATGAA 5 cut(s) 120, 604, 646, 667, 918
TspRI CASTG 4 cut(s) 184, 456, 595, 673
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 1 cut(s) 843
XceI RCATGY 1 cut(s) 634
XspI CTAG 3 cut(s) 38, 476, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.