Rh1AG423000

Ferric reduction oxidase 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001860
Physical Location & Seq
Forward (+)
65237961 .. 65248496
10536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG423000.1

Sequence Viewer

Length: 3153 bp
ATGAACAACATGATCATCAGGTTGATATTGCTTCTGGTGTTTGTTGGATGGCTCATAGTTTGGATTATGGTGCCAACAAAAACTTACAAATATACCTGGAAACCCAACCTTGACGCCAATCTCACGTCAACTTATTTTGGAGTGCAAGGGACAAATCTGCTGCTTTTCGCTTTCCCAATGATGTTCGTAGCAGCCTTGAGCTGTGTTTATCTTCATCTAGTTCAGAAAAAATCACAAAACTCCAACGAGATCAGGTCGTCGGTTGCTGCTGGCTTCTGGAGACGTCCGGTGGTTGTAACGGCTCCTCTGGGGGTCGTCACTGCGATGGAGCTAGGGTTTGGGGTGATGTTTACTTCACTCTTGGTTTGGTCTCTAGCTAACTACTTGATTACCAGCTTCGGCAACCTCCATATGCATATGAATATGATGAGCATGGATAAAGTATGGCAAAGCAAGTTTCGAAGTGTATCGTTGAGACTTGGGTACATTGGGAACATATGCTGGGCGTTTCTCTTCTTTCCTGTGACTCGAAGCTCTTCGATTTTACACATGTTTGGGCTCACATCGGAGTCGAGTATAAAGTACCATATTTGGCTTGGCCATCTCTCCATGCTTCTATTTTCTGTACACACCGTAGGCTTCATCATCTACTGGGGCATGACTCATCAAATGGCTGAAATGCTAGAATGGAGCTCGACTTATGTATCAAACGTAGCTGGAGAGATTGCAATTGTGTTTGCAATGGCAATGTGGGTGACAAGCATTCCTCGTGTCAGACGGAAAATGTTTGAAGTATTTTTCTATACGCACCAGCTATACCCACTCTATATCTTGTTCTACGTATTACATGTTGGTGCTTCCTATTTCTGTATGATCCTTCCCGGAATCTTCCTCTTTGTCATTGATCGATACTTGAGATTTTTACAGTCTTCCCAACACGCTGCTTTAGTCTCAGCACGGGTTCTACCTTGTGAAACAATTGAGCTCAACTTCTCCAGGAGCAAAGGATTGGATTATAATCCTACTAGCATCTTGTTCGTCAATGTGCCTAGCATATCAAAGTTGCAATGGCACCCATTTACAGTGAATTCAAACTCTAACATGGAGGCGGACAAAATAAGTGTTGTGATCAAGAGTTTAGGAAGTTGGTCTCAAAAGCTTTATAAGCAACTGGCTTCTAATTCGGTGGAGCATATCCAAGTTTCTGTTGAAGGACCTTATGGACCTACTTCATCTCATTTTATGAGGCACAAGGGTTTAGTGATGGTAAGTGGAGGCAGTGGAATTACTCCTATGATTTCCATAATCCGAGAAATTATTTTCCAAAGCACTAAACCCGACTGCCATGTTCCAAGTGTACGGCTGATTTGTGCATTCAAGAACTCAGTTGATCTCTCACTGCTGCAACTATTGCTTCCACTTTCTGGCACGCCTTCTGACCTTACCCAGATACAATTGCAAATAGAAGCCTATGTTACAAGGGAGACAGAGCAGCAGCCTCCATCTTCCCAAAAGCCATCCCAAACTTTATGGTTTAAGCCAAACCCATTGGATTTACCGATTTCTGGTGTTCTAGGTCCAAAAAGTTGGTTATGGCTTGCTGCTATTATCTCATCCTCATTTGTCTTATTTCTCATTATTTTGGGCATGGTCACTCGTTTCTATATATACCCGATTGACCACAACACTGGTGATAGTTACCATTACTCCTTCACTTGCCTTTGGTACATGTTTTTGGTCTGTGCCTCCATTTTTATAGTCTCTAGTACAGTTTTCATCTGGTGCAAGAACCAAAATGCCATGGAAAGAAATCAAATTCAGAATATAGAAGTACCCACAGATACAATTGATGATAGAGAACTTGAAAGCTTTCCCCTTCATCATGAGTCCCTGATTGCTCAAGCTACCCAGGTGCATTATGGAACAAGGCCTGATCTCAAGAAAATTCTGTTGGAAACTAAAGGATCTGATATCGGAGTGTTGGCTTGTGGCCCAAAGAAGATGAGACATGAGGTTGCCAAACTTTGTTCATCCGGTTTGGCACAGAATCTGCACTTTGAGTCTATTAGCTTTAACTGCGAGAGTAGTGGTCCTCTTTTCACTTCTGAAGCCATGAGCACAAATACAGCAGTAGTATCAGTTATGAGGTTCATATTGCTTCTAATGTTTGTTGGATGGCTCATCGTTTGGATTCTGTTGCCAACCAAAGCTTACGGATATTCCTGGAGACCCAACCTACAAGGCAAACTCAACTCAACATATTTCGGAGATAAAGGGACAAATCTGCTGCTTTTCTCGTTCCCAATGATGTTCATAGCAGCTTTGAGTTGTGTTTATCTCCATCTAATTCAGAAGGAGAAACCATCATACTCGACATACTACTCCACAAGTTTTGCTAGCTTCTGGAGGCGTCCGGCGAGTGTGAAAGCTCCACTGGGAATTGTAACTACGATGGAGCTTGGATTCGCTGTAATGTTTGTTGCACTCTTGGTGTGGTCTCTTACCAATTACATACATGTCGGGTTTGCCTACCTCCATAATATGTATAAGAAACCAGTTCCGGGCCTCAAAGAGAAAGAGTGGGAAGCAAAGCTTCATTATGTATCGTACTGGCTTGGCTTAACCGGAAACATATGTCTGGCATTTCTCTTCTTCCCTGTAACTCGAGGCTCTTCGATTTTGGCTCTGGTTGGGTTAACATCGGAGTCCAGTATGAAGTACCATATTTGGCTTGGCCATCTCTCCATGGTTCTATTTGCTGCTCATTCCCTAGGCTACATCATCTTCTGGGCCATCACTCATCAAATGGCTGAGATGCTAGAATGGAGCAAGACTTATGTATCAAACGTAGCTGGGGAGATTGCAATTGTATTTGCAATGGCAATGTGGGTGACTAGCATTCCTCGTGTTAGACGAAAAATGTTTGAACTATTTTTCTACACCCACCAGCTCTACGTTCTCTACATCGTCTTCTACGTACTGCACGCCGGTGCTTTCTCCTTCTGTACAATCCTTCCTGGAATCTTCCTGTTCATCATAGATCGGTACCTGAGATTTTTACAGTCTAATCAACGTGCTGAGTTACTCTCTGCACGCATTTTACCTTGTGAGACTATTGAACTCAACTTCTCCAAGAGCCAAGGTGATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

1050

Amino Acids

119.58

Weight (kDa)

9.1

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferric_reduct PF01794 160 - 279 2.1e-18 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 315 - 411 1.5e-23 FAD-binding domain
NAD_binding_6 PF08030 419 - 677 5e-21 Ferric reductase NAD binding domain
Ferric_reduct PF01794 872 - 992 2.7e-19 Ferric reductase like transmembrane component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1017, 1164
AatII GACGTC 1 cut(s) 286
Acc65I GGTACC 1 cut(s) 3045
AccB1I GGYRCC 3 cut(s) 70, 1071, 3045
AccB7I CCANNNNNTGG 1 cut(s) 1424
AciI CCGC 1 cut(s) 1109
AclWI GGATC 2 cut(s) 868, 1972
AcoI YGGCCR 2 cut(s) 598, 2734
AcsI RAATTY 3 cut(s) 1087, 1815, 1944
AcuI CTGAAG 1 cut(s) 2127
AcyI GRCGYC 3 cut(s) 114, 283, 2410
AfiI CCNNNNNNNGG 3 cut(s) 1424, 1565, 2561
AflIII ACRYGT 4 cut(s) 549, 847, 1728, 2515
AgsI TTSAA 7 cut(s) 791, 1092, 1211, 1378, 1865, 2927, 3119
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 5 cut(s) 95, 995, 1908, 2222, 3016
AjuI GAANNNNNNNTTGG 2 cut(s) 1934, 1966
AleI CACNNNNGTG 1 cut(s) 2988
Alw21I GWGCWC 3 cut(s) 695, 987, 2120
AlwI GGATC 2 cut(s) 868, 1972
AlwNI CAGNNNCTG 1 cut(s) 2050
Ama87I CYCGRG 1 cut(s) 2664
AoxI GGCC 6 cut(s) 598, 1928, 1990, 2563, 2734, 2790
ApoI RAATTY 3 cut(s) 1087, 1815, 1944
Asp700I GAANNNNTTC 2 cut(s) 535, 1869
Asp718I GGTACC 1 cut(s) 3045
AspA2I CCTAGG 1 cut(s) 2770
AspS9I GGNCC 7 cut(s) 1214, 1223, 1577, 1991, 2090, 2563, 2790
AsuC2I CCSGG 2 cut(s) 882, 2562
AsuHPI GGTGA 4 cut(s) 355, 766, 1703, 2902
AsuII TTCGAA 1 cut(s) 460
AsuNHI GCTAGC 1 cut(s) 2396
AvaI CYCGRG 1 cut(s) 2664
AvaII GGWCC 4 cut(s) 1214, 1223, 1577, 2090
AvrII CCTAGG 1 cut(s) 2770
BaeI ACNNNNGTAYC 4 cut(s) 687, 720, 2823, 2856
BalI TGGCCA 2 cut(s) 600, 2736
BanI GGYRCC 3 cut(s) 70, 1071, 3045
BanII GRGCYC 3 cut(s) 561, 695, 987
BauI CACGAG 2 cut(s) 768, 2904
BbsI GAAGAC 2 cut(s) 921, 2962
Bbv12I GWGCWC 3 cut(s) 695, 987, 2120
BceAI ACGGC 2 cut(s) 315, 1376
BcgI CGANNNNNNTGC 2 cut(s) 1018, 1052
BciT130I CCWGG 5 cut(s) 97, 997, 1910, 2224, 3018
BclI TGATCA 2 cut(s) 12, 1128
BcnI CCSGG 2 cut(s) 882, 2562
BlnI CCTAGG 1 cut(s) 2770
Bme1390I CCNGG 7 cut(s) 97, 882, 997, 1910, 2224, 2562, 3018
Bme18I GGWCC 4 cut(s) 1214, 1223, 1577, 2090
BmeT110I CYCGRG 1 cut(s) 2664
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 7 cut(s) 1214, 1223, 1577, 1991, 2090, 2563, 2790
BmiI GGNNCC 4 cut(s) 72, 303, 1073, 3047
BmrFI CCNGG 7 cut(s) 97, 882, 997, 1910, 2224, 2562, 3018
BmrI ACTGGG 2 cut(s) 661, 2444
BmsI GCATC 2 cut(s) 1038, 2805
BmtI GCTAGC 1 cut(s) 2400
BmuI ACTGGG 2 cut(s) 661, 2444
BpiI GAAGAC 2 cut(s) 921, 2962
BplI GAGNNNNNCTC 2 cut(s) 3071, 3103
BpmI CTGGAG 5 cut(s) 298, 738, 979, 2245, 2425
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 4 cut(s) 217, 934, 1884, 1922
BpuMI CCSGG 2 cut(s) 882, 2562
Bsa29I ATCGAT 1 cut(s) 907
BsaAI YACGTR 2 cut(s) 841, 2977
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 3 cut(s) 114, 283, 2410
BsaI GGTCTC 4 cut(s) 375, 1155, 2221, 2502
BsaJI CCNNGG 5 cut(s) 1800, 1908, 2745, 2770, 3139
BsaWI WCCGGW 3 cut(s) 286, 2033, 2624
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 1691, 1721
Bsc4I CCNNNNNNNGG 3 cut(s) 1424, 1565, 2561
Bse118I RCCGGY 1 cut(s) 2987
Bse1I ACTGG 7 cut(s) 656, 1176, 1693, 2439, 2555, 2615, 2709
Bse3DI GCAATG 5 cut(s) 747, 753, 1073, 2883, 2889
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 5 cut(s) 97, 997, 1910, 2224, 3018
BseCI ATCGAT 1 cut(s) 907
BseDI CCNNGG 5 cut(s) 1800, 1908, 2745, 2770, 3139
BseGI GGATG 5 cut(s) 53, 1517, 1613, 2030, 2180
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 3 cut(s) 1424, 1565, 2561
BseMI GCAATG 5 cut(s) 747, 753, 1073, 2883, 2889
BseMII CTCAG 5 cut(s) 966, 1398, 2802, 3041, 3069
BseNI ACTGG 7 cut(s) 656, 1176, 1693, 2439, 2555, 2615, 2709
BseRI GAGGAG 1 cut(s) 294
BseYI CCCAGC 2 cut(s) 501, 2852
BsgI GTGCAG 3 cut(s) 2036, 2966, 3075
BshFI GGCC 6 cut(s) 600, 1930, 1992, 2565, 2736, 2792
BshNI GGYRCC 3 cut(s) 70, 1071, 3045
BshVI ATCGAT 1 cut(s) 907
BsiHKAI GWGCWC 3 cut(s) 695, 987, 2120
BsiHKCI CYCGRG 1 cut(s) 2664
BsiSI CCGG 7 cut(s) 287, 882, 2034, 2414, 2561, 2625, 2988
BslFI GGGAC 3 cut(s) 163, 1873, 2290
BslI CCNNNNNNNGG 3 cut(s) 1424, 1565, 2561
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 3 cut(s) 163, 1873, 2290
BsmI GAATGC 3 cut(s) 762, 1373, 2898
BsnI GGCC 6 cut(s) 600, 1930, 1992, 2565, 2736, 2792
Bso31I GGTCTC 4 cut(s) 375, 1155, 2221, 2502
BsoBI CYCGRG 1 cut(s) 2664
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 4 cut(s) 561, 695, 987, 2120
Bsp1407I TGTACA 2 cut(s) 625, 3005
Bsp143I GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
Bsp19I CCATGG 2 cut(s) 1800, 2745
BspACI CCGC 1 cut(s) 1109
BspANI GGCC 6 cut(s) 600, 1930, 1992, 2565, 2736, 2792
BspCNI CTCAG 5 cut(s) 965, 1397, 2803, 3042, 3070
BspDI ATCGAT 1 cut(s) 907
BspHI TCATGA 1 cut(s) 1882
BspLI GGNNCC 4 cut(s) 72, 303, 1073, 3047
BspOI GCTAGC 1 cut(s) 2400
BspPI GGATC 2 cut(s) 868, 1972
BspQI GCTCTTC 2 cut(s) 541, 2677
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 3 cut(s) 70, 1071, 3045
BspTNI GGTCTC 4 cut(s) 375, 1155, 2221, 2502
BsrDI GCAATG 5 cut(s) 747, 753, 1073, 2883, 2889
BsrFI RCCGGY 1 cut(s) 2987
BsrGI TGTACA 2 cut(s) 625, 3005
BsrI ACTGG 7 cut(s) 656, 1176, 1693, 2439, 2555, 2615, 2709
BssAI RCCGGY 1 cut(s) 2987
BssECI CCNNGG 5 cut(s) 1800, 1908, 2745, 2770, 3139
BssMI GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
BssNI GRCGYC 3 cut(s) 114, 283, 2410
BssSI CACGAG 2 cut(s) 768, 2904
BssT1I CCWWGG 4 cut(s) 1800, 2745, 2770, 3139
Bst2BI CACGAG 2 cut(s) 768, 2904
Bst2UI CCWGG 5 cut(s) 97, 997, 1910, 2224, 3018
Bst4CI ACNGT 5 cut(s) 634, 927, 1084, 1771, 3063
Bst6I CTCTTC 4 cut(s) 518, 541, 2654, 2677
BstACI GRCGYC 3 cut(s) 114, 283, 2410
BstAPI GCANNNNNTGC 1 cut(s) 1411
BstAUI TGTACA 2 cut(s) 625, 3005
BstBAI YACGTR 2 cut(s) 841, 2977
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 6 cut(s) 271, 1430, 1599, 2398, 2985, 3094
BstDEI CTNAG 5 cut(s) 952, 1384, 2811, 3050, 3078
BstDSI CCRYGG 2 cut(s) 1800, 2745
BstF5I GGATG 5 cut(s) 53, 1517, 1613, 2030, 2180
BstKTI GATC 9 cut(s) 15, 252, 876, 907, 1131, 1393, 1936, 1967, 3043
BstMBI GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
BstMWI GCNNNNNNNGC 3 cut(s) 1165, 1411, 2076
BstNI CCWGG 5 cut(s) 97, 997, 1910, 2224, 3018
BstNSI RCATGY 4 cut(s) 553, 851, 1732, 2519
BstSCI CCNGG 7 cut(s) 95, 880, 995, 1908, 2222, 2560, 3016
BstSNI TACGTA 2 cut(s) 841, 2977
BstV2I GAAGAC 2 cut(s) 921, 2962
BstX2I RGATCY 1 cut(s) 1964
BstXI CCANNNNNNTGG 2 cut(s) 1587, 1688
BstYI RGATCY 1 cut(s) 1964
Bsu15I ATCGAT 1 cut(s) 907
BsuRI GGCC 6 cut(s) 600, 1930, 1992, 2565, 2736, 2792
BsuTUI ATCGAT 1 cut(s) 907
BtgI CCRYGG 2 cut(s) 1800, 2745
BtgZI GCGATG 1 cut(s) 338
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 5 cut(s) 53, 1517, 1613, 2030, 2180
BtsI GCAGTG 3 cut(s) 318, 1285, 1397
BtsIMutI CAGTG 6 cut(s) 318, 1089, 1285, 1397, 1686, 2432
Cac8I GCNNGC 6 cut(s) 271, 1430, 1599, 2398, 2985, 3094
CaiI CAGNNNCTG 1 cut(s) 2050
CciI TCATGA 1 cut(s) 1882
Cfr10I RCCGGY 1 cut(s) 2987
Cfr13I GGNCC 7 cut(s) 1214, 1223, 1577, 1991, 2090, 2563, 2790
ClaI ATCGAT 1 cut(s) 907
CseI GACGC 2 cut(s) 122, 2399
CspCI CAANNNNNGTGG 2 cut(s) 2374, 2409
DdeI CTNAG 5 cut(s) 952, 1384, 2811, 3050, 3078
DpnI GATC 9 cut(s) 14, 251, 875, 906, 1130, 1392, 1935, 1966, 3042
DpnII GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
EaeI YGGCCR 2 cut(s) 598, 2734
Eam1104I CTCTTC 4 cut(s) 518, 541, 2654, 2677
EarI CTCTTC 4 cut(s) 518, 541, 2654, 2677
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 2 cut(s) 693, 985
Eco105I TACGTA 2 cut(s) 841, 2977
Eco130I CCWWGG 4 cut(s) 1800, 2745, 2770, 3139
Eco147I AGGCCT 1 cut(s) 1930
Eco24I GRGCYC 3 cut(s) 561, 695, 987
Eco31I GGTCTC 4 cut(s) 375, 1155, 2221, 2502
Eco32I GATATC 1 cut(s) 1972
Eco47I GGWCC 4 cut(s) 1214, 1223, 1577, 2090
Eco53kI GAGCTC 2 cut(s) 693, 985
Eco57I CTGAAG 1 cut(s) 2127
Eco88I CYCGRG 1 cut(s) 2664
EcoICRI GAGCTC 2 cut(s) 693, 985
EcoO109I RGGNCCY 1 cut(s) 1214
EcoRI GAATTC 1 cut(s) 1087
EcoRII CCWGG 5 cut(s) 95, 995, 1908, 2222, 3016
EcoRV GATATC 1 cut(s) 1972
EcoT14I CCWWGG 4 cut(s) 1800, 2745, 2770, 3139
EcoT22I ATGCAT 1 cut(s) 417
EcoT38I GRGCYC 3 cut(s) 561, 695, 987
ErhI CCWWGG 4 cut(s) 1800, 2745, 2770, 3139
Esp3I CGTCTC 1 cut(s) 274
FalI AAGNNNNNCTT 2 cut(s) 2577, 2609
FaqI GGGAC 3 cut(s) 163, 1873, 2290
FauNDI CATATG 4 cut(s) 411, 417, 497, 2633
FbaI TGATCA 2 cut(s) 12, 1128
FokI GGATG 5 cut(s) 60, 1504, 1600, 2017, 2187
FriOI GRGCYC 3 cut(s) 561, 695, 987
GsaI CCCAGC 2 cut(s) 505, 2856
GsuI CTGGAG 5 cut(s) 298, 738, 979, 2245, 2425
HaeIII GGCC 6 cut(s) 600, 1930, 1992, 2565, 2736, 2792
HapII CCGG 7 cut(s) 287, 882, 2034, 2414, 2561, 2625, 2988
HgaI GACGC 2 cut(s) 122, 2399
Hin1I GRCGYC 3 cut(s) 114, 283, 2410
HincII GTYRAC 2 cut(s) 129, 2697
HindII GTYRAC 2 cut(s) 129, 2697
HindIII AAGCTT 4 cut(s) 1157, 1867, 2208, 2591
HpaI GTTAAC 1 cut(s) 2697
HpaII CCGG 7 cut(s) 287, 882, 2034, 2414, 2561, 2625, 2988
HphI GGTGA 4 cut(s) 355, 766, 1703, 2902
Hpy166II GTNNAC 5 cut(s) 129, 351, 628, 1358, 2697
Hpy188III TCNNGA 6 cut(s) 277, 1132, 1378, 1883, 1939, 2404
Hpy8I GTNNAC 5 cut(s) 129, 351, 628, 1358, 2697
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 8 cut(s) 887, 1205, 1443, 1720, 1886, 2347, 3010, 3023
HpyCH4III ACNGT 5 cut(s) 634, 927, 1084, 1771, 3063
HpyCH4IV ACGT 8 cut(s) 125, 283, 711, 840, 2847, 2955, 2976, 3073
HpyF10VI GCNNNNNNNGC 3 cut(s) 1165, 1411, 2076
HpyF3I CTNAG 5 cut(s) 952, 1384, 2811, 3050, 3078
HpySE526I ACGT 8 cut(s) 125, 283, 711, 840, 2847, 2955, 2976, 3073
Hsp92I GRCGYC 3 cut(s) 114, 283, 2410
KpnI GGTACC 1 cut(s) 3049
Ksp22I TGATCA 2 cut(s) 12, 1128
KspAI GTTAAC 1 cut(s) 2697
Kzo9I GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
LguI GCTCTTC 2 cut(s) 541, 2677
LmnI GCTCC 8 cut(s) 307, 328, 690, 999, 1189, 2434, 2455, 2826
LweI GCATC 2 cut(s) 1038, 2805
MaeII ACGT 8 cut(s) 125, 283, 711, 840, 2847, 2955, 2976, 3073
MalI GATC 9 cut(s) 14, 251, 875, 906, 1130, 1392, 1935, 1966, 3042
MboI GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
MfeI CAATTG 5 cut(s) 729, 978, 1454, 1845, 2865
MflI RGATCY 1 cut(s) 1964
MhlI GDGCHC 4 cut(s) 561, 695, 987, 2120
MlsI TGGCCA 2 cut(s) 600, 2736
MluNI TGGCCA 2 cut(s) 600, 2736
MlyI GAGTC 6 cut(s) 520, 578, 655, 1895, 2069, 2714
MmeI TCCRAC 4 cut(s) 25, 267, 1932, 2152
Mox20I TGGCCA 2 cut(s) 600, 2736
Mph1103I ATGCAT 1 cut(s) 417
MroXI GAANNNNTTC 2 cut(s) 535, 1869
MscI TGGCCA 2 cut(s) 600, 2736
MseI TTAA 4 cut(s) 1536, 2073, 2621, 2696
MslI CAYNNNNRTG 3 cut(s) 323, 852, 2988
Msp20I TGGCCA 2 cut(s) 600, 2736
MspI CCGG 7 cut(s) 287, 882, 2034, 2414, 2561, 2625, 2988
MspR9I CCNGG 7 cut(s) 97, 882, 997, 1910, 2224, 2562, 3018
MunI CAATTG 5 cut(s) 729, 978, 1454, 1845, 2865
Mva1269I GAATGC 3 cut(s) 762, 1373, 2898
MvaI CCWGG 5 cut(s) 97, 997, 1910, 2224, 3018
MwoI GCNNNNNNNGC 3 cut(s) 1165, 1411, 2076
NciI CCSGG 2 cut(s) 882, 2562
NcoI CCATGG 2 cut(s) 1800, 2745
NdeI CATATG 4 cut(s) 411, 417, 497, 2633
NdeII GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
NheI GCTAGC 1 cut(s) 2396
NlaIV GGNNCC 4 cut(s) 72, 303, 1073, 3047
NmuCI GTSAC 5 cut(s) 316, 523, 754, 1651, 2890
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 4 cut(s) 553, 851, 1732, 2519
NspV TTCGAA 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 2988
PaeR7I CTCGAG 1 cut(s) 2664
PagI TCATGA 1 cut(s) 1882
PceI AGGCCT 1 cut(s) 1930
PciI ACATGT 4 cut(s) 549, 847, 1728, 2515
PciSI GCTCTTC 2 cut(s) 541, 2677
PcsI WCGNNNNNNNCGW 2 cut(s) 2911, 2973
PctI GAATGC 3 cut(s) 762, 1373, 2898
PdmI GAANNNNTTC 2 cut(s) 535, 1869
PfeI GAWTC 5 cut(s) 885, 2047, 2191, 2463, 3021
PflMI CCANNNNNTGG 1 cut(s) 1424
PfoI TCCNGGA 4 cut(s) 880, 995, 2222, 3016
PleI GAGTC 6 cut(s) 520, 577, 655, 1894, 2068, 2713
PpsI GAGTC 6 cut(s) 520, 577, 655, 1894, 2068, 2713
Ppu21I YACGTR 2 cut(s) 841, 2977
PpuMI RGGWCCY 1 cut(s) 1214
PscI ACATGT 4 cut(s) 549, 847, 1728, 2515
PsiI TTATAA 2 cut(s) 1017, 1164
Psp124BI GAGCTC 2 cut(s) 695, 987
Psp5II RGGWCCY 1 cut(s) 1214
Psp6I CCWGG 5 cut(s) 95, 995, 1908, 2222, 3016
PspFI CCCAGC 2 cut(s) 501, 2852
PspGI CCWGG 5 cut(s) 95, 995, 1908, 2222, 3016
PspN4I GGNNCC 4 cut(s) 72, 303, 1073, 3047
PspPI GGNCC 7 cut(s) 1214, 1223, 1577, 1991, 2090, 2563, 2790
PspPPI RGGWCCY 1 cut(s) 1214
PspXI VCTCGAGB 1 cut(s) 2664
PstNI CAGNNNCTG 1 cut(s) 2050
PsuI RGATCY 1 cut(s) 1964
RseI CAYNNNNRTG 3 cut(s) 323, 852, 2988
SacI GAGCTC 2 cut(s) 695, 987
SapI GCTCTTC 2 cut(s) 541, 2677
SaqAI TTAA 4 cut(s) 1536, 2073, 2621, 2696
Sau3AI GATC 9 cut(s) 12, 249, 873, 904, 1128, 1390, 1933, 1964, 3040
Sau96I GGNCC 7 cut(s) 1214, 1223, 1577, 1991, 2090, 2563, 2790
SchI GAGTC 6 cut(s) 520, 578, 655, 1895, 2069, 2714
ScrFI CCNGG 7 cut(s) 97, 882, 997, 1910, 2224, 2562, 3018
SduI GDGCHC 4 cut(s) 561, 695, 987, 2120
SfaNI GCATC 2 cut(s) 1038, 2805
Sfr274I CTCGAG 1 cut(s) 2664
SfuI TTCGAA 1 cut(s) 460
SgrAI CRCCGGYG 1 cut(s) 2987
SinI GGWCC 4 cut(s) 1214, 1223, 1577, 2090
SlaI CTCGAG 1 cut(s) 2664
SmiMI CAYNNNNRTG 3 cut(s) 323, 852, 2988
SmlI CTYRAG 5 cut(s) 196, 913, 1899, 1937, 2664
SmoI CTYRAG 5 cut(s) 196, 913, 1899, 1937, 2664
SnaBI TACGTA 2 cut(s) 841, 2977
SseBI AGGCCT 1 cut(s) 1930
SsiI CCGC 1 cut(s) 1109
SstI GAGCTC 2 cut(s) 695, 987
StuI AGGCCT 1 cut(s) 1930
StyD4I CCNGG 7 cut(s) 95, 880, 995, 1908, 2222, 2560, 3016
StyI CCWWGG 4 cut(s) 1800, 2745, 2770, 3139
TaaI ACNGT 5 cut(s) 634, 927, 1084, 1771, 3063
TaiI ACGT 8 cut(s) 128, 286, 714, 843, 2850, 2958, 2979, 3076
TaqI TCGA 9 cut(s) 460, 529, 539, 572, 695, 907, 2372, 2665, 2675
TatI WGTACW 3 cut(s) 625, 1766, 3005
TfiI GAWTC 5 cut(s) 885, 2047, 2191, 2463, 3021
Tru1I TTAA 4 cut(s) 1536, 2073, 2621, 2696
Tru9I TTAA 4 cut(s) 1536, 2073, 2621, 2696
TscAI CASTG 6 cut(s) 325, 1089, 1285, 1404, 1693, 2439
TseFI GTSAC 5 cut(s) 316, 523, 754, 1651, 2890
Tsp45I GTSAC 5 cut(s) 316, 523, 754, 1651, 2890
TspGWI ACGGA 2 cut(s) 793, 2229
TspRI CASTG 6 cut(s) 325, 1089, 1285, 1404, 1693, 2439
Van91I CCANNNNNTGG 1 cut(s) 1424
VpaK11BI GGWCC 4 cut(s) 1214, 1223, 1577, 2090
XapI RAATTY 3 cut(s) 1087, 1815, 1944
XceI RCATGY 4 cut(s) 553, 851, 1732, 2519
XcmI CCANNNNNNNNNTGG 3 cut(s) 593, 1916, 2729
XhoI CTCGAG 1 cut(s) 2664
XmaJI CCTAGG 1 cut(s) 2770
XmnI GAANNNNTTC 2 cut(s) 535, 1869
ZraI GACGTC 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.