Rroxscaffold_4G00280710

Ferric reduction oxidase 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001860
Physical Location & Seq
Forward (+)
3179667 .. 3180610
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280710.1

Sequence Viewer

Length: 819 bp
ATGGTAAGTGGAGGCAGTGGAATTACTCCTATGATTTCCATAATCCGAGAAATTATTTTCCAAAGCACTAAACCCGACTGCCATGTTCCAAGTGTACGGCTGATATGTGCATTCAAGAACTCAGCTGATCTCTCACTGCTGCAACTATTGCTTCCACTTTCTGGCACGCCTTCTGACCTTACCCAGATACAATTGCAAATAGAAGCCTATGTTACAAGGGAGACAGAGCAGCAGCCTCCATCTTCCCAGAAGCCATCCCAAACTTTATGGTTCAAGCCAAACCCATTGGATTTACCGATTTCTGGTGTTCTAGGTCCAAAAAGTTGGTTATGGCTTGCTGCTATTATCTCATCCTCATTTGTCTTATTTCTCTTTGTTTTGGGCATGGTCACTCGTTTCTATATATACCCGATTGACCACAACACTGGTGATAGTTACCATTACTCCTTCACTTGCCTTTGGTACATGTTTTTGGTCTGTGCCTCCATTTTTATACTCTCTAGTACAGTTTTCATCTGGTGCAAGAACCAAAATGCCATGGAAAGAAATCAAATTCAGAATGTAGAAGTACCCACAGAAATAATTGATGATAGAGAACTTGAAAGCTTTCCCCTTCATCATGAGTCCCTGATTGCTCAAGCTACCCAGGTGCACTATGGAACAAGGCCTGATCTCAAGAAAATTCTGTTGGAAACTAAAGGATCTGATATCGGAGTGTTGGCTTGTGGCCCAAAGAAGATGAGACATGAGGTTGCCAAACTTTGTTCATCCGGTTTGGCACAGAATCTGCACTTTGAGTCTATTAGCTTTAACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

272

Amino Acids

30.62

Weight (kDa)

6.23

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_6 PF08030 1 - 256 1.5e-21 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 161
AclWI GGATC 1 cut(s) 709
AcsI RAATTY 2 cut(s) 552, 681
AfaI GTAC 4 cut(s) 96, 464, 505, 570
AfiI CCNNNNNNNGG 2 cut(s) 161, 302
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 3 cut(s) 115, 274, 602
AjnI CCWGG 1 cut(s) 645
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 4 cut(s) 125, 606, 641, 807
AluI AGCT 4 cut(s) 125, 606, 641, 807
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 215, 736
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 1 cut(s) 709
AlwNI CAGNNNCTG 1 cut(s) 787
AoxI GGCC 2 cut(s) 665, 727
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 4 cut(s) 139, 229, 232, 338
ApoI RAATTY 2 cut(s) 552, 681
Asp700I GAANNNNTTC 1 cut(s) 606
AspS9I GGNCC 2 cut(s) 314, 728
AsuHPI GGTGA 1 cut(s) 440
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 4 cut(s) 126, 241, 244, 325
BccI CCATC 2 cut(s) 247, 262
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 647
BcoDI GTCTC 2 cut(s) 215, 736
BfaI CTAG 2 cut(s) 311, 501
BisI GCNGC 4 cut(s) 140, 230, 233, 339
BlsI GCNGC 4 cut(s) 141, 231, 234, 340
Bme1390I CCNGG 1 cut(s) 647
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 314, 728
BmrFI CCNGG 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 621, 659
BsaJI CCNNGG 2 cut(s) 537, 645
BsaWI WCCGGW 1 cut(s) 770
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 302
Bse1I ACTGG 2 cut(s) 430, 818
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 2 cut(s) 537, 645
BseGI GGATG 3 cut(s) 254, 350, 767
BseLI CCNNNNNNNGG 2 cut(s) 161, 302
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 2 cut(s) 430, 818
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 4 cut(s) 126, 241, 244, 325
BsgI GTGCAG 1 cut(s) 773
BshFI GGCC 2 cut(s) 667, 729
BsiHKAI GWGCWC 1 cut(s) 654
BsiSI CCGG 1 cut(s) 771
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 2 cut(s) 161, 302
BsmAI GTCTC 2 cut(s) 215, 736
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 2 cut(s) 667, 729
Bsp1286I GDGCHC 1 cut(s) 654
Bsp143I GATC 3 cut(s) 127, 670, 701
Bsp19I CCATGG 1 cut(s) 537
BspANI GGCC 2 cut(s) 667, 729
BspCNI CTCAG 1 cut(s) 134
BspHI TCATGA 1 cut(s) 619
BspPI GGATC 1 cut(s) 709
BsrI ACTGG 2 cut(s) 430, 818
BssECI CCNNGG 2 cut(s) 537, 645
BssMI GATC 3 cut(s) 127, 670, 701
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 508
BstAPI GCANNNNNTGC 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 167, 336
BstDEI CTNAG 1 cut(s) 121
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 3 cut(s) 254, 350, 767
BstKTI GATC 3 cut(s) 130, 673, 704
BstMAI GTCTC 2 cut(s) 215, 736
BstMBI GATC 3 cut(s) 127, 670, 701
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 4 cut(s) 126, 241, 244, 325
BstX2I RGATCY 1 cut(s) 701
BstXI CCANNNNNNTGG 2 cut(s) 324, 425
BstYI RGATCY 1 cut(s) 701
BsuRI GGCC 2 cut(s) 667, 729
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 3 cut(s) 254, 350, 767
BtsI GCAGTG 2 cut(s) 22, 134
BtsIMutI CAGTG 3 cut(s) 22, 134, 423
Cac8I GCNNGC 2 cut(s) 167, 336
CaiI CAGNNNCTG 1 cut(s) 787
CciI TCATGA 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 314, 728
Csp6I GTAC 4 cut(s) 95, 463, 504, 569
CviAII CATG 6 cut(s) 83, 385, 466, 538, 620, 746
CviQI GTAC 4 cut(s) 95, 463, 504, 569
DdeI CTNAG 1 cut(s) 121
DpnI GATC 3 cut(s) 129, 672, 703
DpnII GATC 3 cut(s) 127, 670, 701
Eco130I CCWWGG 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 667
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 645
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 6 cut(s) 86, 388, 469, 541, 623, 749
FaqI GGGAC 1 cut(s) 610
FatI CATG 6 cut(s) 82, 384, 465, 537, 619, 745
Fnu4HI GCNGC 4 cut(s) 140, 230, 233, 339
FokI GGATG 3 cut(s) 241, 337, 754
Fsp4HI GCNGC 4 cut(s) 140, 230, 233, 339
FspBI CTAG 2 cut(s) 311, 501
GluI GCNGC 4 cut(s) 140, 230, 233, 339
HaeIII GGCC 2 cut(s) 667, 729
HapII CCGG 1 cut(s) 771
Hin1II CATG 6 cut(s) 86, 388, 469, 541, 623, 749
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 3 cut(s) 623, 784, 797
HpaII CCGG 1 cut(s) 771
HphI GGTGA 1 cut(s) 440
Hpy166II GTNNAC 2 cut(s) 95, 652
Hpy188I TCNGA 5 cut(s) 47, 175, 558, 706, 713
Hpy188III TCNNGA 3 cut(s) 115, 620, 676
Hpy8I GTNNAC 2 cut(s) 95, 652
HpyAV CCTTC 3 cut(s) 180, 457, 623
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4V TGCA 6 cut(s) 110, 142, 196, 522, 652, 790
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 121
Hsp92II CATG 6 cut(s) 86, 388, 469, 541, 623, 749
Kzo9I GATC 3 cut(s) 127, 670, 701
Lsp1109I GCAGC 4 cut(s) 126, 241, 244, 325
MaeI CTAG 2 cut(s) 311, 501
MaeIII GTNAC 3 cut(s) 211, 388, 434
MalI GATC 3 cut(s) 129, 672, 703
MboI GATC 3 cut(s) 127, 670, 701
MboII GAAGA 2 cut(s) 234, 748
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 6 cut(s) 21, 51, 191, 552, 582, 681
MlyI GAGTC 2 cut(s) 632, 806
MmeI TCCRAC 1 cut(s) 669
MnlI CCTC 5 cut(s) 5, 246, 364, 493, 742
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 1 cut(s) 810
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 771
MspR9I CCNGG 1 cut(s) 647
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 148
NcoI CCATGG 1 cut(s) 537
NdeII GATC 3 cut(s) 127, 670, 701
NlaIII CATG 6 cut(s) 86, 388, 469, 541, 623, 749
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 469
PagI TCATGA 1 cut(s) 619
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 465
PctI GAATGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 1 cut(s) 784
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 4 cut(s) 141, 231, 234, 340
PleI GAGTC 2 cut(s) 631, 805
PpsI GAGTC 2 cut(s) 631, 805
PscI ACATGT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspPI GGNCC 2 cut(s) 314, 728
PstNI CAGNNNCTG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 4 cut(s) 96, 464, 505, 570
RsaNI GTAC 4 cut(s) 95, 463, 504, 569
SaqAI TTAA 1 cut(s) 810
SatI GCNGC 4 cut(s) 140, 230, 233, 339
Sau3AI GATC 3 cut(s) 127, 670, 701
Sau96I GGNCC 2 cut(s) 314, 728
SchI GAGTC 2 cut(s) 632, 806
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 1 cut(s) 654
SetI ASST 8 cut(s) 127, 180, 316, 608, 643, 651, 753, 809
SinI GGWCC 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 636, 674
SmoI CTYRAG 2 cut(s) 636, 674
Sse9I AATT 6 cut(s) 21, 51, 191, 552, 582, 681
SseBI AGGCCT 1 cut(s) 667
SspMI CTAG 2 cut(s) 311, 501
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 1 cut(s) 645
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 1 cut(s) 508
TasI AATT 6 cut(s) 21, 51, 191, 552, 582, 681
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 1 cut(s) 784
Tru1I TTAA 1 cut(s) 810
Tru9I TTAA 1 cut(s) 810
TscAI CASTG 3 cut(s) 22, 141, 430
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 4 cut(s) 139, 229, 232, 338
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 3 cut(s) 502, 605, 756
TspRI CASTG 3 cut(s) 22, 141, 430
Van91I CCANNNNNTGG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 2 cut(s) 552, 681
XceI RCATGY 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 2 cut(s) 311, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.