Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006006

Physical Location & Seq

Genomic Coordinates Reverse (-)

4713421 .. 4722456

Gene Structure 9036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00081730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4245 bp

ATGAAGAGAATACGAGACGATGTGTACGGTGGTAGTGGTGGTGGTGGTGGTTCTTCTCAATTGAAACGGCCGGTTGTTGCTTCTTCGCGCGGTGACTCAAATGGGCCACCCCAACTCCAAGTACAAGGAGGAGGAGGCGCGGTAGGTGGAGTAATAGCTACCGTTGGAGTAGCTACTGTTGGAGTAGGTGCCTCTCAGAAGTTGACGACGAATGATGCTCTTACATACCTCAAAGAAGTGAAGGACATGTTTCAAGACCAAAGAGAAAAGTATGATATGTTTCTTGAGGTCATGAAGGATTTCAAGGCTCAAAGAACTGACACGACAGGTGTCATTGCAAGAGTAAAGGAACTGTTTAAAGGGCATACCAAGTTAATTTTGGGGTTTAATACCTTCTTGCCAAAGGGTTATGAGATCACTCTTGAGGATGTGGAACCAAAAAAGACTGTTGAGTTCGAAGAAGCTATCAGTTTTGTAAACAAGATCAAGAAACGTTTCCAAAATGATGAGCAAGTATACAAGTCATTCTTAGACATACTGAATATGTATCGAAAGGAGCACAAGGACATAAATGAGGTTTACCAGGAGGTTGCTTCTCTATTTGATGACCAACCAGATTTGCTGGATGAGTTCACCAGATTTTTACCAGATACGTCAGCAACAACTTCAACTCATCAGGGGCAATATGGCCGCAATGCATATCCCCGTTTTAATGAGCGGAGCTCTGCTACACCAACTTTGCGGCCAATGCCTATTGACAAGCAACGTAGGCGGGAGAAGATTACTTCTCATGGTGATCATGATATTAGTGTTGATCGTCCTGAGCTAGATGATGACAAAGGAATGATGAAAGTACTGAAGGAGCAGAGAAAGCGTTTTCCTGAGAAGGAGAATAGGGATAGGAGAATTCGTGACCATGAAGATAGAGAAGTAGAGACTGATAACAACAGGGACTACAACTTGCAGCGTTTTCCAGAGAAAAGGAAATCTTCCAGGAAGGTTGAAGGTTTTGGAGCGAATGCTAACTTTGCTCCTTATGATGACAAAGATACCTTGAAGAGTAAGTACAGCCAGGCATTCGGTTTCTGTGAGAAAGTTAAGGAGCGCTTGTGCAGCCAAGATGACTACCAGGCATTTTTGAAGTGCCTTCACATTTATAGCAATGGCATTATTAAAAGGAATGATTTACAAAATTTGGTGACTGATTTATTGGGGAAGTATCCAGATCTTATGGACGAATTTAATGATTTCTTGGAGCGCTGTGAGAATATTGATGGGTTCCTTGCTGGTGTAATGAGAAAATCTCTGAGTAGTGATGGTCATTTATCGCGATCAGTGAAGGTAGAGGACAAAGACAAGGAGCCAAAGCGTGAAATGGAGGGAGTTAAAGAAAAGGAAAGATACAGGGAGAAGTATTGGGCGAAATCTATTCAAGAGCTTGACCTCTCTAACTGTGAACGCTGTACTCCAAGCTATCGCCTTCTTCCAGAAGATTATCCAATACCCTCAGCAAGCCAGAGATCAGAACTTGCTGCTCAGGTCTTGAATGACCATTGGGTATCTGTGACTTCAGGAAGTGAAGATTATTCTTTTAAACACATGCGCAGAAATCAATATGAAGAAAGTCTTTTCCGTTGTGAAGATGATAGGTTTGAGCTAGATATGTTGTTGGAGTCAGTAAGCTCAACTTCTAAGCGAGCAGAGGAACTGTTGAACAGCATGAATGAAAATAAACTCAGTATGGAAACTCAAATTCATATCGAAGACCACTTTACTGCTCTAAACTTAAGGTGCATTGAACGTTTATATGGTGACCATGGTCTTGATGTGATGGACATACTGCGGAAAAGTCCAACGCTTGCTCTGCCAGTCATATTAACTCGCCTGAAACAGAAACAAGAGGAATGGACAAGGTGTAGAGCGGATTTCAACAAGGTTTGGGCTGATATATATGCCAAAAACCATTACAAATCACTTGATCACCGAAGCTTTTATTTCAAGCAACAAGATTCAAAGAACTTGAGCAGCAAATATTTGGTGGCTGAGATCAAGGAATTGAAAGAGAAAAAACAGATAGAGGACGATATTCTTCTGGCTGTTGCTGCTGGAAACAGGCAATCTATAGTTCCACATCTGGAGTATGAATACCTTGATATCAGCATTCATGAGGACTTGTATAAACTTGTCGAATATTCATGTGACGAGCTTAGCTCAACAAAAGAACAGTTAAATAAGGCTATGAGACTTTATACTACCTTTTTGGAGCCAATGTTGGGCATTCCTTCTCGGCCTCATGGTTCAGAGGATGATGAAGATGTGGACAAAGCTAGGAAGGGCGCTATGAATTGTACTGCATCAAGCAATGGAGAAAGTGATGGTAGTCCCGGTGGTGAGACTACCATGGTAAATTTTAAGCAGCCTAAATCTGGGGGTAATGAAGATGAAAATGCTTTAGCAGATGTGGCAAGTTCTAGGAATAGTTTGGCTAATGGGGATACCTTAGCCAAAGAAGATGGCAGTTGCGATGCAGATAATGCCTGCAGGGATGATTCAATGTGTAATAACATTCGGGTAGAGAAAGAAGTCAAAGAACAGAAGAATATTAGTATCCCTGATAAAATGCATGGGTCAAGTAAACCGGTTGCCTCCATTGATCGAGTAGGCAATTCCAATGCATCATTTGCAGTTGGAGGAGAAAATAACCATGGAAGAATCAGCTTGGAAGTGACATCAGGGTCTGTTGCAACTACCTCAAGACCCTACGACTCCATTAGTGAAAATGAGCAATCAAAGAAAACTAATGCTGATCCTGCTGTTCCTTCATCAGAGGGTGGTGATATTGTAAAACCTGCTTCATTTGGTAATGGGGTGTTTAAAGAAAGCACGAAAGTCAACAGTCGTCATGAAGAGTCTGTTGGGCCCTCTAAAATTGAGAAGGAAGAAGGTGAATTATCACCGGTCGGTGATTATGGGGAGGATAACTTCATTGTCTCCGGAGACGCTGGGGTGCAGGCCTTGCCTAAGGAAAACCATGGTGTTGAAAGACAATATCAATCTGGAAACAGGGAAGAAATTTGCTCTCAGGATGCTGGGGAAAATGATGCAGATGCTGATGATGAAAACAGTGAAAATGTTTCTGAGGCTGGTGAAGATGCCTCTGGCAGTGAGACAGCTGGTGATGAGTGTTCCCGAGAAGAGCATGGAGAGGAGGAGGATGCTGAACATGATGACGTTGATGGTAAGGCTGAGAGTGAAGGTGAGGCTGAAGGGATGGCTGATGGGCACCTTGTTGGAGATGGCTGTTCTGTGCAATTTCCAGAACGTTTTCTTATGTCTGTGAAGCCTCTTGCAAAGCATGTATCGGCACCTTTAGTTGACGAAAAGAAAGACTCTCGTGTTTTTTATGGAAATGATAACTTCTATGTGCTCTATAGGCTTCATCAAATTTTATACGAAAGGATTTTATCTGCAAAATCAAATTCAGTAGGAGCAGAAACTAAGTGGAGAACTTCCAAGGATGGCAACCCTCCGGATCTTTATGGCAGGTTTATGGGTGCACTGTACAATTTGCTTGATGGATCTGCTGATAATGCAAAATTTGAGGATGAGTGCCGAGCTATTATTGGAAATCAGTCGTACATCTTATTCACGTTGGACAAGTTGATATATAAATTTGTTAAACAGCTTCAAACGGTTGCAACTGATGAGATGGACAATAAGCTTCTTCAGTTATATGAATATGAAAAGTCTCGGAAAAAAGGGAAGCTAATTGATTCTGTCTATTTTGAAAACACACGTGTCCTTGTTCATGAGGAGAACATTTACAGATTGGAATTCTTCTCTGCACCCTCCCGGCTGTCCATCCAGCTGATGGACAGCGTGAGTGAAAAGCCTGAGGCGTCGGCAGTTTCTATGGAACCTAATTTTTCATCTTATCTGCACAATGATTTCCTGTCACTTTATCCTGGCAAAAAGGAGCCACATGGAATTACCCTGCAGAGAAACAAGCGCAAATATGCAGGGCAAGATGAATCCTCGGCGTTTTCCAATGCCATGGAGGGTGTTCAATTAGTCAATGGGTTGGAGTGTAAAATTGCTTGCAACTCATCCAAGATCTCTTATGTTCTCGACACAGAAGATTACTTCTTCCGTATGAGAAGGAAAAGGAGAAAGTCAGAGTCCAGATCACCATATTGTGATCAGACGAGGGTACAGCGGTTCCACAAATTCTTGTCCGTCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0035601 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0098732 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1414

Amino Acids

160.09

Weight (kDa)

5.25

Isoelectric Point (pI)

44.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	89 - 133	3e-17	Paired amphipathic helix repeat
PAH	PF02671	171 - 215	4.5e-18	Paired amphipathic helix repeat
PAH	PF02671	375 - 418	6.1e-15	Paired amphipathic helix repeat
Sin3_corepress	PF08295	486 - 578	2.4e-34	Sin3 family co-repressor
Sin3a_C	PF16879	1134 - 1382	8.3e-47	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006006)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr0c44g0503731 RchiOBHm_Chr0c44g0503741 RchiOBHm_Chr2g0169931 RchiOBHm_Chr2g0169991 RchiOBHm_Chr2g0170001
rosa_laevigata	RLG00000021911
rosa_multiflora	Rmu_co8017160.1_g000001 Rmu_sc0013078.1_g000017 Rmu_ssc0000389.1_g000009
rosa_roxburghii	Rroxscaffold_2G00081730
rosa_rugosa	Rorug02G0544200 Rorug02G0544300 Rorug02G0544400 Rorug02G0545100
rosa_samantha	Rh2AG616500 Rh2AG617200 Rh2BG628300 Rh2BG628600 Rh2CG597600 Rh2CG598100 Rh2DG639300 Rh2DG640200 Rh4CG011500 Rh4CG012100 Rh4CG012500
rosa_wichuraiana	Rw2G051060 Rw2G051110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2734
Acc16I	TGCGCA	1 cut(s)	1604
Acc36I	ACCTGC	2 cut(s)	2857, 3534
AccB1I	GGYRCC	3 cut(s)	188, 3282, 3364
AccB7I	CCANNNNNTGG	1 cut(s)	3871
AccBSI	CCGCTC	2 cut(s)	718, 1922
AccI	GTMKAC	1 cut(s)	516
AccII	CGCG	4 cut(s)	88, 90, 140, 1330
AccIII	TCCGGA	2 cut(s)	2993, 3529
AciI	CCGC	9 cut(s)	90, 140, 691, 718, 742, 772, 1843, 1922, 4216
AclI	AACGTT	3 cut(s)	493, 1801, 3322
AclWI	GGATC	3 cut(s)	2801, 3540, 3586
AcoI	YGGCCR	3 cut(s)	68, 688, 743
AcuI	CTGAAG	4 cut(s)	878, 1554, 3285, 3710
AcvI	CACGTG	1 cut(s)	3797
AcyI	GRCGYC	1 cut(s)	3899
AfaI	GTAC	9 cut(s)	26, 123, 855, 1067, 1465, 2350, 3563, 3638, 4212
AfeI	AGCGCT	2 cut(s)	1106, 1259
AfiI	CCNNNNNNNGG	3 cut(s)	2273, 2426, 3871
AflII	CTTAAG	1 cut(s)	1786
AflIII	ACRYGT	3 cut(s)	246, 3794, 3796
AgeI	ACCGGT	2 cut(s)	2638, 2956
AjnI	CCWGG	5 cut(s)	582, 992, 1071, 1128, 3964
AjuI	GAANNNNNNNTTGG	2 cut(s)	2898, 2930
Alw21I	GWGCWC	4 cut(s)	561, 725, 3429, 3559
Alw26I	GTCTC	8 cut(s)	9, 929, 2236, 2387, 2991, 2995, 3161, 3753
Alw44I	GTGCAC	1 cut(s)	3555
AlwI	GGATC	3 cut(s)	2801, 3540, 3586
AlwNI	CAGNNNCTG	2 cut(s)	2738, 3110
Ama87I	CYCGRG	1 cut(s)	3189
Aor13HI	TCCGGA	2 cut(s)	2993, 3529
Aor51HI	AGCGCT	2 cut(s)	1106, 1259
AoxI	GGCC	7 cut(s)	68, 104, 688, 743, 2288, 2918, 3012
ApaI	GGGCCC	1 cut(s)	2922
ApaLI	GTGCAC	1 cut(s)	3555
ApeKI	GCWGC	6 cut(s)	964, 1113, 1532, 2025, 2102, 2416
AsiGI	ACCGGT	2 cut(s)	2638, 2956
Asp700I	GAANNNNTTC	3 cut(s)	299, 494, 3324
AspLEI	GCGC	7 cut(s)	90, 140, 1107, 1260, 1605, 2339, 4011
AspS9I	GGNCC	3 cut(s)	104, 2918, 2919
AsuC2I	CCSGG	2 cut(s)	2385, 3853
AsuII	TTCGAA	1 cut(s)	456
AvaI	CYCGRG	1 cut(s)	3189
AxyI	CCTNAGG	2 cut(s)	3021, 3894
BaeGI	GKGCMC	3 cut(s)	2922, 3285, 3559
BaeI	ACNNNNGTAYC	2 cut(s)	4202, 4235
BanI	GGYRCC	3 cut(s)	188, 3282, 3364
BanII	GRGCYC	2 cut(s)	725, 2922
BarI	GAAGNNNNNNTAC	2 cut(s)	1049, 1081
BauI	CACGAG	1 cut(s)	3393
BbrPI	CACGTG	1 cut(s)	3797
BbsI	GAAGAC	1 cut(s)	1770
Bbv12I	GWGCWC	4 cut(s)	561, 725, 3429, 3559
BbvCI	CCTCAGC	1 cut(s)	1507
BbvI	GCAGC	6 cut(s)	976, 1125, 1519, 2037, 2089, 2428
BceAI	ACGGC	1 cut(s)	83
BciT130I	CCWGG	5 cut(s)	584, 994, 1073, 1130, 3966
BciVI	GTATCC	3 cut(s)	1230, 2488, 2618
BclI	TGATCA	3 cut(s)	796, 1978, 4198
BcnI	CCSGG	2 cut(s)	2385, 3853
BcoDI	GTCTC	8 cut(s)	9, 929, 2236, 2387, 2991, 2995, 3161, 3753
BfaI	CTAG	4 cut(s)	827, 1658, 2328, 2472
BfmI	CTRYAG	4 cut(s)	2121, 2539, 3430, 3995
BfoI	RGCGCY	3 cut(s)	1108, 1261, 2340
BfrI	CTTAAG	1 cut(s)	1786
BfuAI	ACCTGC	2 cut(s)	2857, 3534
BfuI	GTATCC	3 cut(s)	1230, 2488, 2618
BglII	AGATCT	2 cut(s)	1225, 4113
BisI	GCNGC	8 cut(s)	691, 743, 965, 1114, 1533, 2026, 2103, 2417
BlpI	GCTNAGC	1 cut(s)	2207
BlsI	GCNGC	8 cut(s)	692, 744, 966, 1115, 1534, 2027, 2104, 2418
BmcAI	AGTACT	1 cut(s)	855
Bme1390I	CCNGG	7 cut(s)	584, 994, 1073, 1130, 2385, 3853, 3966
BmeT110I	CYCGRG	1 cut(s)	3189
BmgT120I	GGNCC	3 cut(s)	104, 2918, 2919
BmrFI	CCNGG	7 cut(s)	584, 994, 1073, 1130, 2385, 3853, 3966
BmsI	GCATC	9 cut(s)	205, 2363, 2515, 2684, 3076, 3091, 3097, 3142, 3205
BoxI	GACNNNNGTC	2 cut(s)	329, 1818
BpiI	GAAGAC	1 cut(s)	1770
BplI	GAGNNNNNCTC	6 cut(s)	707, 739, 1288, 1320, 2195, 2227
BpmI	CTGGAG	1 cut(s)	2156
Bpu10I	CCTNAGC	4 cut(s)	822, 1507, 1536, 2500
Bpu1102I	GCTNAGC	1 cut(s)	2207
Bpu14I	TTCGAA	1 cut(s)	456
BpuEI	CTTGAG	4 cut(s)	305, 443, 2041, 2737
BpuMI	CCSGG	2 cut(s)	2385, 3853
BsaAI	YACGTR	1 cut(s)	3797
BsaHI	GRCGYC	1 cut(s)	3899
BsaJI	CCNNGG	7 cut(s)	1816, 2400, 2704, 3031, 3513, 4035, 4053
BsaWI	WCCGGW	4 cut(s)	2638, 2956, 2993, 3529
Bsc4I	CCNNNNNNNGG	3 cut(s)	2273, 2426, 3871
Bse118I	RCCGGY	3 cut(s)	70, 2638, 2956
Bse1I	ACTGG	1 cut(s)	1868
Bse21I	CCTNAGG	2 cut(s)	3021, 3894
Bse3DI	GCAATG	4 cut(s)	333, 700, 1168, 2368
BseAI	TCCGGA	2 cut(s)	2993, 3529
BseBI	CCWGG	5 cut(s)	584, 994, 1073, 1130, 3966
BseDI	CCNNGG	7 cut(s)	1816, 2400, 2704, 3031, 3513, 4035, 4053
BseLI	CCNNNNNNNGG	3 cut(s)	2273, 2426, 3871
BseMI	GCAATG	4 cut(s)	333, 700, 1168, 2368
BseNI	ACTGG	1 cut(s)	1868
BseRI	GAGGAG	6 cut(s)	144, 147, 2706, 3221, 3224, 3827
BseSI	GKGCMC	3 cut(s)	2922, 3285, 3559
BseX3I	CGGCCG	1 cut(s)	68
BseXI	GCAGC	6 cut(s)	976, 1125, 1519, 2037, 2089, 2428
BseYI	CCCAGC	2 cut(s)	3002, 3089
BsgI	GTGCAG	4 cut(s)	1132, 3029, 3828, 3923
Bsh1236I	CGCG	4 cut(s)	88, 90, 140, 1330
Bsh1285I	CGRYCG	2 cut(s)	71, 2961
BshFI	GGCC	7 cut(s)	70, 106, 690, 745, 2290, 2920, 3014
BshNI	GGYRCC	3 cut(s)	188, 3282, 3364
BshTI	ACCGGT	2 cut(s)	2638, 2956
BsiEI	CGRYCG	2 cut(s)	71, 2961
BsiHKAI	GWGCWC	4 cut(s)	561, 725, 3429, 3559
BsiHKCI	CYCGRG	1 cut(s)	3189
BsiSI	CCGG	7 cut(s)	71, 2385, 2639, 2957, 2994, 3530, 3853
BslFI	GGGAC	2 cut(s)	965, 2367
BslI	CCNNNNNNNGG	3 cut(s)	2273, 2426, 3871
BsmAI	GTCTC	8 cut(s)	9, 929, 2236, 2387, 2991, 2995, 3161, 3753
BsmBI	CGTCTC	2 cut(s)	9, 2991
BsmFI	GGGAC	2 cut(s)	965, 2367
BsmI	GAATGC	4 cut(s)	1024, 1076, 2160, 2277
BsnI	GGCC	7 cut(s)	70, 106, 690, 745, 2290, 2920, 3014
BsoBI	CYCGRG	1 cut(s)	3189
Bsp119I	TTCGAA	1 cut(s)	456
Bsp120I	GGGCCC	1 cut(s)	2918
Bsp1286I	GDGCHC	6 cut(s)	561, 725, 2922, 3285, 3429, 3559
Bsp13I	TCCGGA	2 cut(s)	2993, 3529
Bsp1407I	TGTACA	1 cut(s)	3561
Bsp1720I	GCTNAGC	1 cut(s)	2207
Bsp19I	CCATGG	5 cut(s)	1816, 2400, 2704, 3031, 4053
Bsp68I	TCGCGA	1 cut(s)	1330
BspACI	CCGC	9 cut(s)	90, 140, 691, 718, 742, 772, 1843, 1922, 4216
BspANI	GGCC	7 cut(s)	70, 106, 690, 745, 2290, 2920, 3014
BspEI	TCCGGA	2 cut(s)	2993, 3529
BspFNI	CGCG	4 cut(s)	88, 90, 140, 1330
BspHI	TCATGA	5 cut(s)	291, 799, 2164, 2902, 3808
BspMAI	CTGCAG	2 cut(s)	2543, 3999
BspMI	ACCTGC	2 cut(s)	2857, 3534
BspPI	GGATC	3 cut(s)	2801, 3540, 3586
BspQI	GCTCTTC	1 cut(s)	3189
BspT104I	TTCGAA	1 cut(s)	456
BspT107I	GGYRCC	3 cut(s)	188, 3282, 3364
BspTI	CTTAAG	1 cut(s)	1786
BsrBI	CCGCTC	2 cut(s)	718, 1922
BsrDI	GCAATG	4 cut(s)	333, 700, 1168, 2368
BsrFI	RCCGGY	3 cut(s)	70, 2638, 2956
BsrGI	TGTACA	1 cut(s)	3561
BsrI	ACTGG	1 cut(s)	1868
BssAI	RCCGGY	3 cut(s)	70, 2638, 2956
BssECI	CCNNGG	7 cut(s)	1816, 2400, 2704, 3031, 3513, 4035, 4053
BssNAI	GTATAC	1 cut(s)	517
BssNI	GRCGYC	1 cut(s)	3899
BssSI	CACGAG	1 cut(s)	3393
BssT1I	CCWWGG	6 cut(s)	1816, 2400, 2704, 3031, 3513, 4053
Bst1107I	GTATAC	1 cut(s)	517
Bst2BI	CACGAG	1 cut(s)	3393
Bst2UI	CCWGG	5 cut(s)	584, 994, 1073, 1130, 3966
Bst6I	CTCTTC	3 cut(s)	1052, 2901, 3189
BstACI	GRCGYC	1 cut(s)	3899
BstAFI	CTTAAG	1 cut(s)	1786
BstAPI	GCANNNNNTGC	3 cut(s)	2534, 2681, 3016
BstAUI	TGTACA	1 cut(s)	3561
BstBAI	YACGTR	1 cut(s)	3797
BstBI	TTCGAA	1 cut(s)	456
BstC8I	GCNNGC	6 cut(s)	1513, 1698, 1860, 2539, 3012, 4099
BstDSI	CCRYGG	5 cut(s)	1816, 2400, 2704, 3031, 4053
BstEII	GGTNACC	1 cut(s)	1811
BstFNI	CGCG	4 cut(s)	88, 90, 140, 1330
BstH2I	RGCGCY	3 cut(s)	1108, 1261, 2340
BstHHI	GCGC	7 cut(s)	90, 140, 1107, 1260, 1605, 2339, 4011
BstMAI	GTCTC	8 cut(s)	9, 929, 2236, 2387, 2991, 2995, 3161, 3753
BstMCI	CGRYCG	2 cut(s)	71, 2961
BstNI	CCWGG	5 cut(s)	584, 994, 1073, 1130, 3966
BstNSI	RCATGY	3 cut(s)	250, 1603, 3359
BstPAI	GACNNNNGTC	2 cut(s)	329, 1818
BstPI	GGTNACC	1 cut(s)	1811
BstSCI	CCNGG	7 cut(s)	582, 992, 1071, 1128, 2383, 3851, 3964
BstSFI	CTRYAG	4 cut(s)	2121, 2539, 3430, 3995
BstSLI	GKGCMC	3 cut(s)	2922, 3285, 3559
BstUI	CGCG	4 cut(s)	88, 90, 140, 1330
BstV1I	GCAGC	6 cut(s)	976, 1125, 1519, 2037, 2089, 2428
BstV2I	GAAGAC	1 cut(s)	1770
BstX2I	RGATCY	4 cut(s)	1225, 3532, 3578, 4113
BstXI	CCANNNNNNTGG	1 cut(s)	4054
BstYI	RGATCY	4 cut(s)	1225, 3532, 3578, 4113
BstZ17I	GTATAC	1 cut(s)	517
BstZI	CGGCCG	1 cut(s)	68
Bsu36I	CCTNAGG	2 cut(s)	3021, 3894
BsuI	GTATCC	3 cut(s)	1230, 2488, 2618
BsuRI	GGCC	7 cut(s)	70, 106, 690, 745, 2290, 2920, 3014
BtgI	CCRYGG	5 cut(s)	1816, 2400, 2704, 3031, 4053
BtgZI	GCGATG	1 cut(s)	2538
BtsI	GCAGTG	1 cut(s)	3169
BtsIMutI	CAGTG	4 cut(s)	1341, 3130, 3169, 3557
BtuMI	TCGCGA	1 cut(s)	1330
BveI	ACCTGC	2 cut(s)	2857, 3534
Cac8I	GCNNGC	6 cut(s)	1513, 1698, 1860, 2539, 3012, 4099
CaiI	CAGNNNCTG	2 cut(s)	2738, 3110
CciI	TCATGA	5 cut(s)	291, 799, 2164, 2902, 3808
CfoI	GCGC	7 cut(s)	90, 140, 1107, 1260, 1605, 2339, 4011
Cfr10I	RCCGGY	3 cut(s)	70, 2638, 2956
Cfr13I	GGNCC	3 cut(s)	104, 2918, 2919
CseI	GACGC	2 cut(s)	3008, 3888
Csp6I	GTAC	9 cut(s)	25, 122, 854, 1066, 1464, 2349, 3562, 3637, 4211
CspAI	ACCGGT	2 cut(s)	2638, 2956
CviQI	GTAC	9 cut(s)	25, 122, 854, 1066, 1464, 2349, 3562, 3637, 4211
DraI	TTTAAA	3 cut(s)	358, 1594, 2875
DrdI	GACNNNNNNGTC	1 cut(s)	2734
DseDI	GACNNNNNNGTC	1 cut(s)	2734
EaeI	YGGCCR	3 cut(s)	68, 688, 743
EagI	CGGCCG	1 cut(s)	68
Eam1104I	CTCTTC	3 cut(s)	1052, 2901, 3189
EarI	CTCTTC	3 cut(s)	1052, 2901, 3189
Ecl136II	GAGCTC	1 cut(s)	723
EclXI	CGGCCG	1 cut(s)	68
Eco130I	CCWWGG	6 cut(s)	1816, 2400, 2704, 3031, 3513, 4053
Eco147I	AGGCCT	1 cut(s)	3014
Eco24I	GRGCYC	2 cut(s)	725, 2922
Eco32I	GATATC	1 cut(s)	2155
Eco47III	AGCGCT	2 cut(s)	1106, 1259
Eco52I	CGGCCG	1 cut(s)	68
Eco53kI	GAGCTC	1 cut(s)	723
Eco57I	CTGAAG	4 cut(s)	878, 1554, 3285, 3710
Eco72I	CACGTG	1 cut(s)	3797
Eco81I	CCTNAGG	2 cut(s)	3021, 3894
Eco88I	CYCGRG	1 cut(s)	3189
Eco91I	GGTNACC	1 cut(s)	1811
EcoICRI	GAGCTC	1 cut(s)	723
EcoO109I	RGGNCCY	1 cut(s)	2919
EcoO65I	GGTNACC	1 cut(s)	1811
EcoRI	GAATTC	2 cut(s)	906, 3833
EcoRII	CCWGG	5 cut(s)	582, 992, 1071, 1128, 3964
EcoRV	GATATC	1 cut(s)	2155
EcoT14I	CCWWGG	6 cut(s)	1816, 2400, 2704, 3031, 3513, 4053
EcoT22I	ATGCAT	3 cut(s)	700, 2625, 2677
EcoT38I	GRGCYC	2 cut(s)	725, 2922
ErhI	CCWWGG	6 cut(s)	1816, 2400, 2704, 3031, 3513, 4053
Esp3I	CGTCTC	2 cut(s)	9, 2991
FaqI	GGGAC	2 cut(s)	965, 2367
FauI	CCCGC	1 cut(s)	765
FbaI	TGATCA	3 cut(s)	796, 1978, 4198
FblI	GTMKAC	1 cut(s)	516
Fnu4HI	GCNGC	8 cut(s)	691, 743, 965, 1114, 1533, 2026, 2103, 2417
FriOI	GRGCYC	2 cut(s)	725, 2922
Fsp4HI	GCNGC	8 cut(s)	691, 743, 965, 1114, 1533, 2026, 2103, 2417
FspBI	CTAG	4 cut(s)	827, 1658, 2328, 2472
FspI	TGCGCA	1 cut(s)	1604
GlaI	GCGC	7 cut(s)	89, 139, 1106, 1259, 1604, 2338, 4010
GluI	GCNGC	8 cut(s)	691, 743, 965, 1114, 1533, 2026, 2103, 2417
GsaI	CCCAGC	2 cut(s)	3006, 3093
GsuI	CTGGAG	1 cut(s)	2156
HaeII	RGCGCY	3 cut(s)	1108, 1261, 2340
HaeIII	GGCC	7 cut(s)	70, 106, 690, 745, 2290, 2920, 3014
HapII	CCGG	7 cut(s)	71, 2385, 2639, 2957, 2994, 3530, 3853
HgaI	GACGC	2 cut(s)	3008, 3888
HhaI	GCGC	7 cut(s)	90, 140, 1107, 1260, 1605, 2339, 4011
Hin1I	GRCGYC	1 cut(s)	3899
Hin6I	GCGC	7 cut(s)	88, 138, 1105, 1258, 1603, 2337, 4009
HinP1I	GCGC	7 cut(s)	88, 138, 1105, 1258, 1603, 2337, 4009
HincII	GTYRAC	3 cut(s)	204, 2893, 3376
HindII	GTYRAC	3 cut(s)	204, 2893, 3376
HindIII	AAGCTT	2 cut(s)	1987, 3719
HpaII	CCGG	7 cut(s)	71, 2385, 2639, 2957, 2994, 3530, 3853
Hpy188I	TCNGA	9 cut(s)	198, 1308, 1525, 2302, 2827, 3139, 3753, 4177, 4203
Hpy99I	CGWCG	2 cut(s)	211, 3904
HpyCH4IV	ACGT	8 cut(s)	493, 653, 766, 1801, 3231, 3322, 3650, 3796
HpySE526I	ACGT	8 cut(s)	493, 653, 766, 1801, 3231, 3322, 3650, 3796
Hsp92I	GRCGYC	1 cut(s)	3899
HspAI	GCGC	7 cut(s)	88, 138, 1105, 1258, 1603, 2337, 4009
Kpn2I	TCCGGA	2 cut(s)	2993, 3529
Ksp22I	TGATCA	3 cut(s)	796, 1978, 4198
LguI	GCTCTTC	1 cut(s)	3189
Lsp1109I	GCAGC	6 cut(s)	976, 1125, 1519, 2037, 2089, 2428
LweI	GCATC	9 cut(s)	205, 2363, 2515, 2684, 3076, 3091, 3097, 3142, 3205
MaeI	CTAG	4 cut(s)	827, 1658, 2328, 2472
MaeII	ACGT	8 cut(s)	493, 653, 766, 1801, 3231, 3322, 3650, 3796
MaeIII	GTNAC	8 cut(s)	92, 911, 1198, 1564, 1811, 2198, 2725, 3954
MbiI	CCGCTC	2 cut(s)	718, 1922
MfeI	CAATTG	1 cut(s)	59
MflI	RGATCY	4 cut(s)	1225, 3532, 3578, 4113
MhlI	GDGCHC	6 cut(s)	561, 725, 2922, 3285, 3429, 3559
MlyI	GAGTC	6 cut(s)	89, 1682, 2759, 2918, 3383, 4187
MmeI	TCCRAC	8 cut(s)	145, 160, 1650, 1877, 2668, 3271, 3633, 4062
Mph1103I	ATGCAT	3 cut(s)	700, 2625, 2677
MroI	TCCGGA	2 cut(s)	2993, 3529
MroXI	GAANNNNTTC	3 cut(s)	299, 494, 3324
MspA1I	CMGCKG	3 cut(s)	3173, 3868, 4216
MspCI	CTTAAG	1 cut(s)	1786
MspI	CCGG	7 cut(s)	71, 2385, 2639, 2957, 2994, 3530, 3853
MspR9I	CCNGG	7 cut(s)	584, 994, 1073, 1130, 2385, 3853, 3966
MunI	CAATTG	1 cut(s)	59
Mva1269I	GAATGC	4 cut(s)	1024, 1076, 2160, 2277
MvaI	CCWGG	5 cut(s)	584, 994, 1073, 1130, 3966
MvnI	CGCG	4 cut(s)	88, 90, 140, 1330
NciI	CCSGG	2 cut(s)	2385, 3853
NcoI	CCATGG	5 cut(s)	1816, 2400, 2704, 3031, 4053
NmeAIII	GCCGAG	3 cut(s)	2266, 3638, 4016
NmuCI	GTSAC	8 cut(s)	92, 911, 1198, 1564, 1811, 2198, 2725, 3954
NruI	TCGCGA	1 cut(s)	1330
NsbI	TGCGCA	1 cut(s)	1604
NsiI	ATGCAT	3 cut(s)	700, 2625, 2677
NspI	RCATGY	3 cut(s)	250, 1603, 3359
NspV	TTCGAA	1 cut(s)	456
PagI	TCATGA	5 cut(s)	291, 799, 2164, 2902, 3808
PceI	AGGCCT	1 cut(s)	3014
PciI	ACATGT	1 cut(s)	246
PciSI	GCTCTTC	1 cut(s)	3189
PctI	GAATGC	4 cut(s)	1024, 1076, 2160, 2277
PdmI	GAANNNNTTC	3 cut(s)	299, 494, 3324
PfeI	GAWTC	5 cut(s)	2009, 2549, 2712, 3773, 4031
PflMI	CCANNNNNTGG	1 cut(s)	3871
PfoI	TCCNGGA	1 cut(s)	992
PinAI	ACCGGT	2 cut(s)	2638, 2956
PkrI	GCNGC	8 cut(s)	692, 744, 966, 1115, 1534, 2027, 2104, 2418
PleI	GAGTC	6 cut(s)	89, 1681, 2759, 2917, 3383, 4186
PmaCI	CACGTG	1 cut(s)	3797
PmlI	CACGTG	1 cut(s)	3797
PpsI	GAGTC	6 cut(s)	89, 1681, 2759, 2917, 3383, 4186
Ppu21I	YACGTR	1 cut(s)	3797
PscI	ACATGT	1 cut(s)	246
PshAI	GACNNNNGTC	2 cut(s)	329, 1818
Psp124BI	GAGCTC	1 cut(s)	725
Psp1406I	AACGTT	3 cut(s)	493, 1801, 3322
Psp6I	CCWGG	5 cut(s)	582, 992, 1071, 1128, 3964
PspCI	CACGTG	1 cut(s)	3797
PspEI	GGTNACC	1 cut(s)	1811
PspFI	CCCAGC	2 cut(s)	3002, 3089
PspGI	CCWGG	5 cut(s)	582, 992, 1071, 1128, 3964
PspOMI	GGGCCC	1 cut(s)	2918
PspPI	GGNCC	3 cut(s)	104, 2918, 2919
PstI	CTGCAG	2 cut(s)	2543, 3999
PstNI	CAGNNNCTG	2 cut(s)	2738, 3110
PsuI	RGATCY	4 cut(s)	1225, 3532, 3578, 4113
PvuII	CAGCTG	2 cut(s)	3173, 3868
RruI	TCGCGA	1 cut(s)	1330
RsaI	GTAC	9 cut(s)	26, 123, 855, 1067, 1465, 2350, 3563, 3638, 4212
RsaNI	GTAC	9 cut(s)	25, 122, 854, 1066, 1464, 2349, 3562, 3637, 4211
SacI	GAGCTC	1 cut(s)	725
SapI	GCTCTTC	1 cut(s)	3189
SatI	GCNGC	8 cut(s)	691, 743, 965, 1114, 1533, 2026, 2103, 2417
Sau96I	GGNCC	3 cut(s)	104, 2918, 2919
SbfI	CCTGCAGG	1 cut(s)	2543
ScaI	AGTACT	1 cut(s)	855
SchI	GAGTC	6 cut(s)	89, 1682, 2759, 2918, 3383, 4187
ScrFI	CCNGG	7 cut(s)	584, 994, 1073, 1130, 2385, 3853, 3966
SdaI	CCTGCAGG	1 cut(s)	2543
SduI	GDGCHC	6 cut(s)	561, 725, 2922, 3285, 3429, 3559
SfaNI	GCATC	9 cut(s)	205, 2363, 2515, 2684, 3076, 3091, 3097, 3142, 3205
SfcI	CTRYAG	4 cut(s)	2121, 2539, 3430, 3995
SfuI	TTCGAA	1 cut(s)	456
SmlI	CTYRAG	5 cut(s)	284, 422, 1786, 2020, 2752
SmoI	CTYRAG	5 cut(s)	284, 422, 1786, 2020, 2752
Sse8387I	CCTGCAGG	1 cut(s)	2543
SseBI	AGGCCT	1 cut(s)	3014
SsiI	CCGC	9 cut(s)	90, 140, 691, 718, 742, 772, 1843, 1922, 4216
SspI	AATATT	4 cut(s)	1270, 2033, 2192, 2602
SspMI	CTAG	4 cut(s)	827, 1658, 2328, 2472
SstI	GAGCTC	1 cut(s)	725
StuI	AGGCCT	1 cut(s)	3014
StyD4I	CCNGG	7 cut(s)	582, 992, 1071, 1128, 2383, 3851, 3964
StyI	CCWWGG	6 cut(s)	1816, 2400, 2704, 3031, 3513, 4053
TaiI	ACGT	8 cut(s)	496, 656, 769, 1804, 3234, 3325, 3653, 3799
TaqI	TCGA	6 cut(s)	456, 550, 1761, 2187, 2656, 4128
TatI	WGTACW	6 cut(s)	121, 853, 1065, 1463, 2348, 3561
TauI	GCSGC	2 cut(s)	693, 745
TfiI	GAWTC	5 cut(s)	2009, 2549, 2712, 3773, 4031
TscAI	CASTG	4 cut(s)	1341, 3130, 3169, 3564
TseFI	GTSAC	8 cut(s)	92, 911, 1198, 1564, 1811, 2198, 2725, 3954
TseI	GCWGC	6 cut(s)	964, 1113, 1532, 2025, 2102, 2416
Tsp45I	GTSAC	8 cut(s)	92, 911, 1198, 1564, 1811, 2198, 2725, 3954
TspGWI	ACGGA	3 cut(s)	1622, 4139, 4225
TspRI	CASTG	4 cut(s)	1341, 3130, 3169, 3564
Van91I	CCANNNNNTGG	1 cut(s)	3871
Vha464I	CTTAAG	1 cut(s)	1786
VneI	GTGCAC	1 cut(s)	3555
XceI	RCATGY	3 cut(s)	250, 1603, 3359
XcmI	CCANNNNNNNNNTGG	2 cut(s)	376, 3868
XmiI	GTMKAC	1 cut(s)	516
XmnI	GAANNNNTTC	3 cut(s)	299, 494, 3324
XspI	CTAG	4 cut(s)	827, 1658, 2328, 2472
ZrmI	AGTACT	1 cut(s)	855
Zsp2I	ATGCAT	3 cut(s)	700, 2625, 2677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.