Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0006006

Physical Location & Seq

Genomic Coordinates Forward (+)

84096716 .. 84106082

Gene Structure 9367 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG616500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4155 bp

ATGAAGAGAATACGAGACGATGTGTACGGTGGTTCTGGTAGTGGTGGTGTTGGTGTCGCTGGTGGTGGTGGTGGTTCTTCTCAATTGAAACGGCCGGTTGTTGCTTCTTCGCGCGGTGACTCAAATGGGCCACCCCAACTCCAAGTACAAGGAGGAGGACGCGCGGTAGGTGAAGTAATAGCTACAGTTGGAGTAGGTGCCTCTCAGAAATTGACTACGAATGATGCACTTACATACCTCAAAGAAGTGAAGGACATGTTTCAAGACCAAAGAGAAAAGTATGATATGTTTCTTGAGGTCATGAAGGATTTCAAGGCTCAAAGAACGGACACGACAGGTGTCATTGCAAGAGTAAAGGAACTGTTTAAAGGGCATACCAAGTTAATTTTGGGGTTTAATACCTTCTTGCCAAAGGGTTATGAGATCACTCTTGAGGAAGTGGAACCAAAAAAGACTGTTGAGTTCGAAGAAGCTATCAGTTTTGTAAACAAGATCAAGAAACGTTTCCAAAATGATGAGCAAGTATACAAGTCATTCTTAGACATACTGAATATGTATCGAAAGGAGCACAAGGACATAAATGAGGTTTACCAGGAGGTTGCTTCTCTATTTGATGACCAACCAGATTTGCTGGATGAGTTCACCAGATTTTTACCAGATACGTCAGCAACAACTTCAACTCATCAGGGGCAATATGGCCGCAATGCATATCCCCGTTTTAATGAGCGGAGCTCTGCTACACCAACTTTGCGGCCAATGCCTATTGACAAGCAACGTAGGCGGGATAAGATTACTTCTCATGGTGATCATGATATTAGTGTTGATCGTCCTGAGCTAGATGATGACAAAGGAATGATGAAAGTACTGAAGGAGCAGAAAAAGCGTTTTCCTGAGAAGGAGAATAGGGATAGGAGAATTCGTGACCATGAAGATAGAGAAGTAGAGACTGATAACAACAGGGACTACAACTTGCAGCATTTTCCAGAGAAAAGGAAATCTTCCAGGAAGGTTGAAGGTTTTGGAGCGAATGCTAACTTTGCTCCTTATGATGACAAAGATACCTTGAAGAGTAAGTACAGCCAGGCATTCGGTTTCTGTGAGAAAGTTAAGGAGCGCTTGTGCAGCCAAGATGACTACCAGGCATTTTTGAAGTGCCTTCACATTTATAGCAATGGCATTATTAAAAGGAATGATTTACAAAATTTGGTGACTGATTTATTGGGGAAGTATCCAGATCTTATGGACGAATTTAATGATTTCTTGGAGCGCTGTGAGAATATTGATGGGTTCCTTGCTGGTGTAATGAGAAAATCTCTGAGTAGTGATGGTCATTTATCGCGATCAGTGAAAGTAGAGGACAAAGACAAGGAGCCAAAGCGTGAAATGGAGGGAGTTAAAGAAAAGGAAAGATACAGGGAGAAGTATTGGGCGAAATCTATTCAAGAGCTTGACCTCTCTAACTGTGAACGCTGTACTCCAAGCTATCGCCTTCTTCCAGAAGATTATCCAATACCCTCAGCAAGCCAGAGATCAGAACTTGCTGCTCAGGTCTTGAATGACCATTGGGTATCTGTGACTTCAGGAAGTGAAGATTATTCTTTTAAACACATGCGCAGAAATCAATATGAAGAAAGTCTTTTCCGTTGTGAAGATGATAGGTTTGAGCTAGATATGTTGTTGGAGTCAGTAAGCTCAACTTCTAAGCGAGCAGAGGAACTGTTGAACAGCATGAATGAAAATAAACTCAGTATGGAAACTCAAATTCACATCGAAGACCACTTTACTGCTCTAAACTTAAGGTGCATTGAACGTTTATATGGTGACCATGGTCTTGATGTGATGGACATACTGCGGAAAAGTCCAACGCTCGCTCTGCCAGTCATATTAACTCGCCTGAAACAGAAACAAGAGGAATGGACAAGGTGTAGAGCGGATTTCAACAAGGTTTGGGCTGATATATATGCCAAAAACCATTACAAATCCCTTGATCACCGAAGCTTTTATTTCAAGCAACAAGATTCAAAGAACTTGAGCAGCAAATATTTGGTGGCTGAGATCAAGGAATTGAAAGAGAAAAAACAGATAGAGGACGATATTCTTCTGGCTGTTGCTGCTGGAAACAGGCAATCTATAGTTCCACATCTGGAGTATGAATACCTTGATATCAGCATTCATGAGGACTTGTATAAACTTGTCGAATATTCATGTGACGAGCTTAGCTCAACAAAAGAACAGTTAAATAAGGCTATGAGACTTTATACTACCTTTTTGGAGCCAATGTTGGGCATTCCTTCTCGGCCTCATGGTTCAGAGGATGATGAAGATGTGGACAAAGCTAGGAAGGGCGCTATGAATTGTACTGCATCAAGCAATGGAGAAAGTGATGGAAGTCCCGGTGGTGAGACTGCCATGGTAAATTTTAAGCAGCCTAAATCTGGGGGTAATGAAGATGAAAATGCTTTAGCAGATGTGGCAAGTTCTAGGAATAGTTTGGCTAATGGGGATACCTTAGCCAAAGAAGATGGCAGTTGCGATGCAGATAATGCCTGCAGGGATGATTCAATGTGTAATAACATTCGGGTAGAGAAAGAAGTCAAAGAACAGAAGAATATTAGTATCCCTGATAAAATGCATGGGTCAAGTAAACCGGTTGCCTCCATTGATCGAGTAGGCAATTCCAATGCATCATTTGCAGTTGGAGGAGAAAATAACCATGGAAGAATCAGCTTGGAAGTGACATCAGGGTCTGTTGCAACTACCTCAAGACCCTACGACTCCATTAGTGAAAATGAGCAATCAAAGAAAACTAATGCTGATCCTGCTGTTCCTTCATCAGAGGGTGGTGATATTGTAAAACCTGCTTCATTTGGTAATGGGGTGTTTACGGAAAGCACTAAAGTCAACAGTCGTCATGAAGAGTCTGTTGGGCCCTCTAAAATTGAGAAGGAAGAAGGTGAATTATCACCAGTCGGTGATTATGGGGAGGATAACTTCGTTGTCTCTGGAGACGCTGGGGTGCAGGCTTTGCCTAAGGAAAACAATGGTGTTGAAAGACAATATCAATCTGGAAACGGGGAAGAAATTTGCTCTCAGGATGCTGGGGAAAATGATGCAGATGCTGATGATGAAAACAGTGAAAATGTTTCTGAGGCTGGTGAAGATGCCTCTGGCAGTGAGACAGCTGGTGATGAGTGTTCCCGAGAAGAGCATGGAGAGGAGGAGGATGCTGAACATGATGACGTTGATGGTAAGGCTGAGAGTGAAGGTGAGGCTGAAGGGATGGCTGATGGGCACCTTGTTGGAGATGGCTGTTCCTTGCAATTGCCAGAACGTTTTCTTATGTCTGTGAAGCCTCTTGCAAAGCATGTATCGGCACCTTTAGTTGACGAAAAGAAAGACTCTCGTGTTTTTTATGGAAATGATAACTTCTATGTGCTCTATAGGCTTCATCAAATTTTATACGAAAGGATTTTATCTGCAAAATCAAATTCAGTAGGAGCAGAAACTAAGTGGAGAACTTCCAAGGATGGCAACCCTCCGGATCTTTATGGCAGGTTTATGGGTGCACTGTACAATTTGCTTGATGGATCTGCTGATAATGCAAAATTTGAGGATGAGTGCCGAGCTATTATTGGAAATCAGTCGTACATCTTATTCACGTTGGACAAGTTAATATATAAATTTGTTAAACAGCTTCAAACGGTTGCAACTGATGAGATGGACAATAAGCTTCTTCAGTTATATGAATATGAAAAGTCTCGGAAAAAAGGGAAGCTAATTGATTCTGTCTATTTTGAAAACACACGTGTCCTTGTTCACGAGGAGAACATTTACAGATTGGAATTCTTCTCTGCACCCTCCCGGCTGTCCATCCAGCTGATGGACAGCGTGAGTGAAAAGCCTGAGGCGTCGGCAGTTTCTATGGAACCTAATTTTTCATCTTATCTGCACAATGATTTCCTGTCACTTTATCCTGGCAAAAAGGAGCCACATGGAATTACCCTGCTGAGAAACAAGCGCAAATATGCAGGGCAAGATGAATCCTCGGCGTTTTCCAATGCCATGGAGGGTGTTCAATTAGTCAATGGGTTGGAGTGTAAAATTGCTTGCAACTCATCCAAGGTATCTCATCTCTTGCCTTTGCATGCTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0035601 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0098732 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202

Pfam Domains

Protein Families

PAH Sin3_corepress Sin3a_C

Protein Analysis

1384

Amino Acids

155.86

Weight (kDa)

5.14

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAH	PF02671	92 - 136	3e-17	Paired amphipathic helix repeat
PAH	PF02671	174 - 218	4.3e-18	Paired amphipathic helix repeat
PAH	PF02671	378 - 421	5.9e-15	Paired amphipathic helix repeat
Sin3_corepress	PF08295	489 - 581	2.3e-34	Sin3 family co-repressor
Sin3a_C	PF16879	1137 - 1375	4.9e-42	C-terminal domain of Sin3a protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0006006)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr0c44g0503731 RchiOBHm_Chr0c44g0503741 RchiOBHm_Chr2g0169931 RchiOBHm_Chr2g0169991 RchiOBHm_Chr2g0170001
rosa_laevigata	RLG00000021911
rosa_multiflora	Rmu_co8017160.1_g000001 Rmu_sc0013078.1_g000017 Rmu_ssc0000389.1_g000009
rosa_roxburghii	Rroxscaffold_2G00081730
rosa_rugosa	Rorug02G0544200 Rorug02G0544300 Rorug02G0544400 Rorug02G0545100
rosa_samantha	Rh2AG616500 Rh2AG617200 Rh2BG628300 Rh2BG628600 Rh2CG597600 Rh2CG598100 Rh2DG639300 Rh2DG640200 Rh4CG011500 Rh4CG012100 Rh4CG012500
rosa_wichuraiana	Rw2G051060 Rw2G051110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2743
Acc16I	TGCGCA	1 cut(s)	1613
Acc36I	ACCTGC	2 cut(s)	2866, 3543
AccB1I	GGYRCC	3 cut(s)	197, 3291, 3373
AccB7I	CCANNNNNTGG	1 cut(s)	3880
AccBSI	CCGCTC	2 cut(s)	727, 1931
AccI	GTMKAC	1 cut(s)	525
AccII	CGCG	5 cut(s)	112, 114, 162, 164, 1339
AccIII	TCCGGA	1 cut(s)	3538
AciI	CCGC	8 cut(s)	114, 164, 700, 727, 751, 781, 1852, 1931
AclI	AACGTT	3 cut(s)	502, 1810, 3331
AclWI	GGATC	3 cut(s)	2810, 3549, 3595
AcoI	YGGCCR	3 cut(s)	92, 697, 752
AcuI	CTGAAG	4 cut(s)	887, 1563, 3294, 3719
AcvI	CACGTG	1 cut(s)	3806
AcyI	GRCGYC	1 cut(s)	3908
AfaI	GTAC	8 cut(s)	26, 147, 864, 1076, 1474, 2359, 3572, 3647
AfeI	AGCGCT	2 cut(s)	1115, 1268
AfiI	CCNNNNNNNGG	3 cut(s)	2282, 2435, 3880
AflII	CTTAAG	1 cut(s)	1795
AflIII	ACRYGT	3 cut(s)	255, 3803, 3805
AgeI	ACCGGT	1 cut(s)	2647
AjnI	CCWGG	5 cut(s)	591, 1001, 1080, 1137, 3973
AjuI	GAANNNNNNNTTGG	2 cut(s)	2907, 2939
Alw21I	GWGCWC	4 cut(s)	570, 734, 3438, 3568
Alw26I	GTCTC	8 cut(s)	9, 938, 2245, 2396, 3000, 3004, 3170, 3762
Alw44I	GTGCAC	1 cut(s)	3564
AlwI	GGATC	3 cut(s)	2810, 3549, 3595
AlwNI	CAGNNNCTG	2 cut(s)	2747, 3119
Ama87I	CYCGRG	1 cut(s)	3198
Aor13HI	TCCGGA	1 cut(s)	3538
Aor51HI	AGCGCT	2 cut(s)	1115, 1268
AoxI	GGCC	6 cut(s)	92, 128, 697, 752, 2297, 2927
ApaI	GGGCCC	1 cut(s)	2931
ApaLI	GTGCAC	1 cut(s)	3564
ApeKI	GCWGC	6 cut(s)	973, 1122, 1541, 2034, 2111, 2425
AsiGI	ACCGGT	1 cut(s)	2647
Asp700I	GAANNNNTTC	3 cut(s)	308, 503, 3333
AspLEI	GCGC	7 cut(s)	114, 164, 1116, 1269, 1614, 2348, 4020
AspS9I	GGNCC	3 cut(s)	128, 2927, 2928
AsuC2I	CCSGG	2 cut(s)	2394, 3862
AsuII	TTCGAA	1 cut(s)	465
AvaI	CYCGRG	1 cut(s)	3198
AxyI	CCTNAGG	2 cut(s)	3030, 3903
BaeGI	GKGCMC	3 cut(s)	2931, 3294, 3568
BanI	GGYRCC	3 cut(s)	197, 3291, 3373
BanII	GRGCYC	2 cut(s)	734, 2931
BarI	GAAGNNNNNNTAC	2 cut(s)	1058, 1090
BauI	CACGAG	2 cut(s)	3402, 3818
BbrPI	CACGTG	1 cut(s)	3806
BbsI	GAAGAC	1 cut(s)	1779
Bbv12I	GWGCWC	4 cut(s)	570, 734, 3438, 3568
BbvCI	CCTCAGC	1 cut(s)	1516
BbvI	GCAGC	6 cut(s)	985, 1134, 1528, 2046, 2098, 2437
BceAI	ACGGC	1 cut(s)	107
BciT130I	CCWGG	5 cut(s)	593, 1003, 1082, 1139, 3975
BciVI	GTATCC	3 cut(s)	1239, 2497, 2627
BclI	TGATCA	2 cut(s)	805, 1987
BcnI	CCSGG	2 cut(s)	2394, 3862
BcoDI	GTCTC	8 cut(s)	9, 938, 2245, 2396, 3000, 3004, 3170, 3762
BfaI	CTAG	4 cut(s)	836, 1667, 2337, 2481
BfmI	CTRYAG	4 cut(s)	183, 2130, 2548, 3439
BfoI	RGCGCY	3 cut(s)	1117, 1270, 2349
BfrI	CTTAAG	1 cut(s)	1795
BfuAI	ACCTGC	2 cut(s)	2866, 3543
BfuI	GTATCC	3 cut(s)	1239, 2497, 2627
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	8 cut(s)	700, 752, 974, 1123, 1542, 2035, 2112, 2426
BlpI	GCTNAGC	1 cut(s)	2216
BlsI	GCNGC	8 cut(s)	701, 753, 975, 1124, 1543, 2036, 2113, 2427
BmcAI	AGTACT	1 cut(s)	864
Bme1390I	CCNGG	7 cut(s)	593, 1003, 1082, 1139, 2394, 3862, 3975
BmeT110I	CYCGRG	1 cut(s)	3198
BmgT120I	GGNCC	3 cut(s)	128, 2927, 2928
BmrFI	CCNGG	7 cut(s)	593, 1003, 1082, 1139, 2394, 3862, 3975
BmsI	GCATC	9 cut(s)	214, 2372, 2524, 2693, 3085, 3100, 3106, 3151, 3214
BoxI	GACNNNNGTC	2 cut(s)	338, 1827
BpiI	GAAGAC	1 cut(s)	1779
BplI	GAGNNNNNCTC	6 cut(s)	716, 748, 1297, 1329, 2204, 2236
BpmI	CTGGAG	2 cut(s)	2165, 3024
Bpu10I	CCTNAGC	4 cut(s)	831, 1516, 1545, 2509
Bpu1102I	GCTNAGC	1 cut(s)	2216
Bpu14I	TTCGAA	1 cut(s)	465
BpuEI	CTTGAG	4 cut(s)	314, 452, 2050, 2746
BpuMI	CCSGG	2 cut(s)	2394, 3862
BsaAI	YACGTR	1 cut(s)	3806
BsaHI	GRCGYC	1 cut(s)	3908
BsaJI	CCNNGG	7 cut(s)	1825, 2409, 2713, 3522, 4044, 4062, 4119
BsaWI	WCCGGW	2 cut(s)	2647, 3538
BsaXI	ACNNNNNCTCC	8 cut(s)	123, 153, 904, 934, 1103, 1133, 2264, 2294
Bsc4I	CCNNNNNNNGG	3 cut(s)	2282, 2435, 3880
Bse118I	RCCGGY	2 cut(s)	94, 2647
Bse1I	ACTGG	2 cut(s)	1877, 2966
Bse21I	CCTNAGG	2 cut(s)	3030, 3903
Bse3DI	GCAATG	4 cut(s)	342, 709, 1177, 2377
BseAI	TCCGGA	1 cut(s)	3538
BseBI	CCWGG	5 cut(s)	593, 1003, 1082, 1139, 3975
BseDI	CCNNGG	7 cut(s)	1825, 2409, 2713, 3522, 4044, 4062, 4119
BseLI	CCNNNNNNNGG	3 cut(s)	2282, 2435, 3880
BseMI	GCAATG	4 cut(s)	342, 709, 1177, 2377
BseNI	ACTGG	2 cut(s)	1877, 2966
BseRI	GAGGAG	5 cut(s)	168, 2715, 3230, 3233, 3836
BseSI	GKGCMC	3 cut(s)	2931, 3294, 3568
BseX3I	CGGCCG	1 cut(s)	92
BseXI	GCAGC	6 cut(s)	985, 1134, 1528, 2046, 2098, 2437
BseYI	CCCAGC	2 cut(s)	3011, 3098
BsgI	GTGCAG	4 cut(s)	1141, 3038, 3837, 3932
Bsh1236I	CGCG	5 cut(s)	112, 114, 162, 164, 1339
Bsh1285I	CGRYCG	1 cut(s)	95
BshFI	GGCC	6 cut(s)	94, 130, 699, 754, 2299, 2929
BshNI	GGYRCC	3 cut(s)	197, 3291, 3373
BshTI	ACCGGT	1 cut(s)	2647
BsiEI	CGRYCG	1 cut(s)	95
BsiHKAI	GWGCWC	4 cut(s)	570, 734, 3438, 3568
BsiHKCI	CYCGRG	1 cut(s)	3198
BsiSI	CCGG	5 cut(s)	95, 2394, 2648, 3539, 3862
BslFI	GGGAC	2 cut(s)	974, 2376
BslI	CCNNNNNNNGG	3 cut(s)	2282, 2435, 3880
BsmAI	GTCTC	8 cut(s)	9, 938, 2245, 2396, 3000, 3004, 3170, 3762
BsmBI	CGTCTC	2 cut(s)	9, 3000
BsmFI	GGGAC	2 cut(s)	974, 2376
BsmI	GAATGC	4 cut(s)	1033, 1085, 2169, 2286
BsnI	GGCC	6 cut(s)	94, 130, 699, 754, 2299, 2929
BsoBI	CYCGRG	1 cut(s)	3198
Bsp119I	TTCGAA	1 cut(s)	465
Bsp120I	GGGCCC	1 cut(s)	2927
Bsp1286I	GDGCHC	6 cut(s)	570, 734, 2931, 3294, 3438, 3568
Bsp13I	TCCGGA	1 cut(s)	3538
Bsp1407I	TGTACA	1 cut(s)	3570
Bsp1720I	GCTNAGC	1 cut(s)	2216
Bsp19I	CCATGG	4 cut(s)	1825, 2409, 2713, 4062
Bsp68I	TCGCGA	1 cut(s)	1339
BspACI	CCGC	8 cut(s)	114, 164, 700, 727, 751, 781, 1852, 1931
BspANI	GGCC	6 cut(s)	94, 130, 699, 754, 2299, 2929
BspEI	TCCGGA	1 cut(s)	3538
BspFNI	CGCG	5 cut(s)	112, 114, 162, 164, 1339
BspHI	TCATGA	4 cut(s)	300, 808, 2173, 2911
BspMAI	CTGCAG	1 cut(s)	2552
BspMI	ACCTGC	2 cut(s)	2866, 3543
BspPI	GGATC	3 cut(s)	2810, 3549, 3595
BspQI	GCTCTTC	1 cut(s)	3198
BspT104I	TTCGAA	1 cut(s)	465
BspT107I	GGYRCC	3 cut(s)	197, 3291, 3373
BspTI	CTTAAG	1 cut(s)	1795
BsrBI	CCGCTC	2 cut(s)	727, 1931
BsrDI	GCAATG	4 cut(s)	342, 709, 1177, 2377
BsrFI	RCCGGY	2 cut(s)	94, 2647
BsrGI	TGTACA	1 cut(s)	3570
BsrI	ACTGG	2 cut(s)	1877, 2966
BssAI	RCCGGY	2 cut(s)	94, 2647
BssECI	CCNNGG	7 cut(s)	1825, 2409, 2713, 3522, 4044, 4062, 4119
BssNAI	GTATAC	1 cut(s)	526
BssNI	GRCGYC	1 cut(s)	3908
BssSI	CACGAG	2 cut(s)	3402, 3818
BssT1I	CCWWGG	6 cut(s)	1825, 2409, 2713, 3522, 4062, 4119
Bst1107I	GTATAC	1 cut(s)	526
Bst2BI	CACGAG	2 cut(s)	3402, 3818
Bst2UI	CCWGG	5 cut(s)	593, 1003, 1082, 1139, 3975
Bst6I	CTCTTC	3 cut(s)	1061, 2910, 3198
BstACI	GRCGYC	1 cut(s)	3908
BstAFI	CTTAAG	1 cut(s)	1795
BstAPI	GCANNNNNTGC	3 cut(s)	2543, 2690, 3025
BstAUI	TGTACA	1 cut(s)	3570
BstBAI	YACGTR	1 cut(s)	3806
BstBI	TTCGAA	1 cut(s)	465
BstC8I	GCNNGC	7 cut(s)	1522, 1707, 1869, 2548, 3021, 4108, 4146
BstDSI	CCRYGG	4 cut(s)	1825, 2409, 2713, 4062
BstEII	GGTNACC	1 cut(s)	1820
BstFNI	CGCG	5 cut(s)	112, 114, 162, 164, 1339
BstH2I	RGCGCY	3 cut(s)	1117, 1270, 2349
BstHHI	GCGC	7 cut(s)	114, 164, 1116, 1269, 1614, 2348, 4020
BstMAI	GTCTC	8 cut(s)	9, 938, 2245, 2396, 3000, 3004, 3170, 3762
BstMCI	CGRYCG	1 cut(s)	95
BstNI	CCWGG	5 cut(s)	593, 1003, 1082, 1139, 3975
BstNSI	RCATGY	4 cut(s)	259, 1612, 3368, 4148
BstPAI	GACNNNNGTC	2 cut(s)	338, 1827
BstPI	GGTNACC	1 cut(s)	1820
BstSCI	CCNGG	7 cut(s)	591, 1001, 1080, 1137, 2392, 3860, 3973
BstSFI	CTRYAG	4 cut(s)	183, 2130, 2548, 3439
BstSLI	GKGCMC	3 cut(s)	2931, 3294, 3568
BstUI	CGCG	5 cut(s)	112, 114, 162, 164, 1339
BstV1I	GCAGC	6 cut(s)	985, 1134, 1528, 2046, 2098, 2437
BstV2I	GAAGAC	1 cut(s)	1779
BstX2I	RGATCY	3 cut(s)	1234, 3541, 3587
BstXI	CCANNNNNNTGG	1 cut(s)	4063
BstYI	RGATCY	3 cut(s)	1234, 3541, 3587
BstZ17I	GTATAC	1 cut(s)	526
BstZI	CGGCCG	1 cut(s)	92
Bsu36I	CCTNAGG	2 cut(s)	3030, 3903
BsuI	GTATCC	3 cut(s)	1239, 2497, 2627
BsuRI	GGCC	6 cut(s)	94, 130, 699, 754, 2299, 2929
BtgI	CCRYGG	4 cut(s)	1825, 2409, 2713, 4062
BtgZI	GCGATG	1 cut(s)	2547
BtsI	GCAGTG	1 cut(s)	3178
BtsIMutI	CAGTG	4 cut(s)	1350, 3139, 3178, 3566
BtuMI	TCGCGA	1 cut(s)	1339
BveI	ACCTGC	2 cut(s)	2866, 3543
Cac8I	GCNNGC	7 cut(s)	1522, 1707, 1869, 2548, 3021, 4108, 4146
CaiI	CAGNNNCTG	2 cut(s)	2747, 3119
CciI	TCATGA	4 cut(s)	300, 808, 2173, 2911
CfoI	GCGC	7 cut(s)	114, 164, 1116, 1269, 1614, 2348, 4020
Cfr10I	RCCGGY	2 cut(s)	94, 2647
Cfr13I	GGNCC	3 cut(s)	128, 2927, 2928
CseI	GACGC	3 cut(s)	168, 3017, 3897
Csp6I	GTAC	8 cut(s)	25, 146, 863, 1075, 1473, 2358, 3571, 3646
CspAI	ACCGGT	1 cut(s)	2647
CviQI	GTAC	8 cut(s)	25, 146, 863, 1075, 1473, 2358, 3571, 3646
DraI	TTTAAA	2 cut(s)	367, 1603
DrdI	GACNNNNNNGTC	1 cut(s)	2743
DseDI	GACNNNNNNGTC	1 cut(s)	2743
EaeI	YGGCCR	3 cut(s)	92, 697, 752
EagI	CGGCCG	1 cut(s)	92
Eam1104I	CTCTTC	3 cut(s)	1061, 2910, 3198
EarI	CTCTTC	3 cut(s)	1061, 2910, 3198
Ecl136II	GAGCTC	1 cut(s)	732
EclXI	CGGCCG	1 cut(s)	92
Eco130I	CCWWGG	6 cut(s)	1825, 2409, 2713, 3522, 4062, 4119
Eco24I	GRGCYC	2 cut(s)	734, 2931
Eco32I	GATATC	1 cut(s)	2164
Eco47III	AGCGCT	2 cut(s)	1115, 1268
Eco52I	CGGCCG	1 cut(s)	92
Eco53kI	GAGCTC	1 cut(s)	732
Eco57I	CTGAAG	4 cut(s)	887, 1563, 3294, 3719
Eco72I	CACGTG	1 cut(s)	3806
Eco81I	CCTNAGG	2 cut(s)	3030, 3903
Eco88I	CYCGRG	1 cut(s)	3198
Eco91I	GGTNACC	1 cut(s)	1820
EcoICRI	GAGCTC	1 cut(s)	732
EcoO109I	RGGNCCY	1 cut(s)	2928
EcoO65I	GGTNACC	1 cut(s)	1820
EcoRI	GAATTC	2 cut(s)	915, 3842
EcoRII	CCWGG	5 cut(s)	591, 1001, 1080, 1137, 3973
EcoRV	GATATC	1 cut(s)	2164
EcoT14I	CCWWGG	6 cut(s)	1825, 2409, 2713, 3522, 4062, 4119
EcoT22I	ATGCAT	3 cut(s)	709, 2634, 2686
EcoT38I	GRGCYC	2 cut(s)	734, 2931
ErhI	CCWWGG	6 cut(s)	1825, 2409, 2713, 3522, 4062, 4119
Esp3I	CGTCTC	2 cut(s)	9, 3000
FaqI	GGGAC	2 cut(s)	974, 2376
FauI	CCCGC	1 cut(s)	774
FbaI	TGATCA	2 cut(s)	805, 1987
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	8 cut(s)	700, 752, 974, 1123, 1542, 2035, 2112, 2426
FriOI	GRGCYC	2 cut(s)	734, 2931
Fsp4HI	GCNGC	8 cut(s)	700, 752, 974, 1123, 1542, 2035, 2112, 2426
FspBI	CTAG	4 cut(s)	836, 1667, 2337, 2481
FspI	TGCGCA	1 cut(s)	1613
GlaI	GCGC	7 cut(s)	113, 163, 1115, 1268, 1613, 2347, 4019
GluI	GCNGC	8 cut(s)	700, 752, 974, 1123, 1542, 2035, 2112, 2426
GsaI	CCCAGC	2 cut(s)	3015, 3102
GsuI	CTGGAG	2 cut(s)	2165, 3024
HaeII	RGCGCY	3 cut(s)	1117, 1270, 2349
HaeIII	GGCC	6 cut(s)	94, 130, 699, 754, 2299, 2929
HapII	CCGG	5 cut(s)	95, 2394, 2648, 3539, 3862
HgaI	GACGC	3 cut(s)	168, 3017, 3897
HhaI	GCGC	7 cut(s)	114, 164, 1116, 1269, 1614, 2348, 4020
Hin1I	GRCGYC	1 cut(s)	3908
Hin6I	GCGC	7 cut(s)	112, 162, 1114, 1267, 1612, 2346, 4018
HinP1I	GCGC	7 cut(s)	112, 162, 1114, 1267, 1612, 2346, 4018
HincII	GTYRAC	2 cut(s)	2902, 3385
HindII	GTYRAC	2 cut(s)	2902, 3385
HindIII	AAGCTT	2 cut(s)	1996, 3728
HpaII	CCGG	5 cut(s)	95, 2394, 2648, 3539, 3862
Hpy188I	TCNGA	7 cut(s)	207, 1317, 1534, 2311, 2836, 3148, 3762
Hpy99I	CGWCG	1 cut(s)	3913
HpyCH4IV	ACGT	8 cut(s)	502, 662, 775, 1810, 3240, 3331, 3659, 3805
HpySE526I	ACGT	8 cut(s)	502, 662, 775, 1810, 3240, 3331, 3659, 3805
Hsp92I	GRCGYC	1 cut(s)	3908
HspAI	GCGC	7 cut(s)	112, 162, 1114, 1267, 1612, 2346, 4018
Kpn2I	TCCGGA	1 cut(s)	3538
Ksp22I	TGATCA	2 cut(s)	805, 1987
LguI	GCTCTTC	1 cut(s)	3198
Lsp1109I	GCAGC	6 cut(s)	985, 1134, 1528, 2046, 2098, 2437
LweI	GCATC	9 cut(s)	214, 2372, 2524, 2693, 3085, 3100, 3106, 3151, 3214
MaeI	CTAG	4 cut(s)	836, 1667, 2337, 2481
MaeII	ACGT	8 cut(s)	502, 662, 775, 1810, 3240, 3331, 3659, 3805
MaeIII	GTNAC	8 cut(s)	116, 920, 1207, 1573, 1820, 2207, 2734, 3963
MbiI	CCGCTC	2 cut(s)	727, 1931
MfeI	CAATTG	2 cut(s)	83, 3320
MflI	RGATCY	3 cut(s)	1234, 3541, 3587
MhlI	GDGCHC	6 cut(s)	570, 734, 2931, 3294, 3438, 3568
MlyI	GAGTC	5 cut(s)	113, 1691, 2768, 2927, 3392
MmeI	TCCRAC	7 cut(s)	169, 1659, 1886, 2677, 3280, 3642, 4071
Mph1103I	ATGCAT	3 cut(s)	709, 2634, 2686
MroI	TCCGGA	1 cut(s)	3538
MroXI	GAANNNNTTC	3 cut(s)	308, 503, 3333
MspA1I	CMGCKG	2 cut(s)	3182, 3877
MspCI	CTTAAG	1 cut(s)	1795
MspI	CCGG	5 cut(s)	95, 2394, 2648, 3539, 3862
MspR9I	CCNGG	7 cut(s)	593, 1003, 1082, 1139, 2394, 3862, 3975
MunI	CAATTG	2 cut(s)	83, 3320
Mva1269I	GAATGC	4 cut(s)	1033, 1085, 2169, 2286
MvaI	CCWGG	5 cut(s)	593, 1003, 1082, 1139, 3975
MvnI	CGCG	5 cut(s)	112, 114, 162, 164, 1339
NciI	CCSGG	2 cut(s)	2394, 3862
NcoI	CCATGG	4 cut(s)	1825, 2409, 2713, 4062
NmeAIII	GCCGAG	3 cut(s)	2275, 3647, 4025
NmuCI	GTSAC	8 cut(s)	116, 920, 1207, 1573, 1820, 2207, 2734, 3963
NruI	TCGCGA	1 cut(s)	1339
NsbI	TGCGCA	1 cut(s)	1613
NsiI	ATGCAT	3 cut(s)	709, 2634, 2686
NspI	RCATGY	4 cut(s)	259, 1612, 3368, 4148
NspV	TTCGAA	1 cut(s)	465
PaeI	GCATGC	1 cut(s)	4148
PagI	TCATGA	4 cut(s)	300, 808, 2173, 2911
PciI	ACATGT	1 cut(s)	255
PciSI	GCTCTTC	1 cut(s)	3198
PctI	GAATGC	4 cut(s)	1033, 1085, 2169, 2286
PdmI	GAANNNNTTC	3 cut(s)	308, 503, 3333
PfeI	GAWTC	5 cut(s)	2018, 2558, 2721, 3782, 4040
PflMI	CCANNNNNTGG	1 cut(s)	3880
PfoI	TCCNGGA	1 cut(s)	1001
PinAI	ACCGGT	1 cut(s)	2647
PkrI	GCNGC	8 cut(s)	701, 753, 975, 1124, 1543, 2036, 2113, 2427
PleI	GAGTC	5 cut(s)	113, 1690, 2768, 2926, 3392
PmaCI	CACGTG	1 cut(s)	3806
PmlI	CACGTG	1 cut(s)	3806
PpsI	GAGTC	5 cut(s)	113, 1690, 2768, 2926, 3392
Ppu21I	YACGTR	1 cut(s)	3806
PscI	ACATGT	1 cut(s)	255
PshAI	GACNNNNGTC	2 cut(s)	338, 1827
Psp124BI	GAGCTC	1 cut(s)	734
Psp1406I	AACGTT	3 cut(s)	502, 1810, 3331
Psp6I	CCWGG	5 cut(s)	591, 1001, 1080, 1137, 3973
PspCI	CACGTG	1 cut(s)	3806
PspEI	GGTNACC	1 cut(s)	1820
PspFI	CCCAGC	2 cut(s)	3011, 3098
PspGI	CCWGG	5 cut(s)	591, 1001, 1080, 1137, 3973
PspOMI	GGGCCC	1 cut(s)	2927
PspPI	GGNCC	3 cut(s)	128, 2927, 2928
PstI	CTGCAG	1 cut(s)	2552
PstNI	CAGNNNCTG	2 cut(s)	2747, 3119
PsuI	RGATCY	3 cut(s)	1234, 3541, 3587
PvuII	CAGCTG	2 cut(s)	3182, 3877
RruI	TCGCGA	1 cut(s)	1339
RsaI	GTAC	8 cut(s)	26, 147, 864, 1076, 1474, 2359, 3572, 3647
RsaNI	GTAC	8 cut(s)	25, 146, 863, 1075, 1473, 2358, 3571, 3646
SacI	GAGCTC	1 cut(s)	734
SapI	GCTCTTC	1 cut(s)	3198
SatI	GCNGC	8 cut(s)	700, 752, 974, 1123, 1542, 2035, 2112, 2426
Sau96I	GGNCC	3 cut(s)	128, 2927, 2928
SbfI	CCTGCAGG	1 cut(s)	2552
ScaI	AGTACT	1 cut(s)	864
SchI	GAGTC	5 cut(s)	113, 1691, 2768, 2927, 3392
ScrFI	CCNGG	7 cut(s)	593, 1003, 1082, 1139, 2394, 3862, 3975
SdaI	CCTGCAGG	1 cut(s)	2552
SduI	GDGCHC	6 cut(s)	570, 734, 2931, 3294, 3438, 3568
SfaNI	GCATC	9 cut(s)	214, 2372, 2524, 2693, 3085, 3100, 3106, 3151, 3214
SfcI	CTRYAG	4 cut(s)	183, 2130, 2548, 3439
SfuI	TTCGAA	1 cut(s)	465
SmlI	CTYRAG	5 cut(s)	293, 431, 1795, 2029, 2761
SmoI	CTYRAG	5 cut(s)	293, 431, 1795, 2029, 2761
SphI	GCATGC	1 cut(s)	4148
Sse8387I	CCTGCAGG	1 cut(s)	2552
SsiI	CCGC	8 cut(s)	114, 164, 700, 727, 751, 781, 1852, 1931
SspI	AATATT	4 cut(s)	1279, 2042, 2201, 2611
SspMI	CTAG	4 cut(s)	836, 1667, 2337, 2481
SstI	GAGCTC	1 cut(s)	734
StyD4I	CCNGG	7 cut(s)	591, 1001, 1080, 1137, 2392, 3860, 3973
StyI	CCWWGG	6 cut(s)	1825, 2409, 2713, 3522, 4062, 4119
TaiI	ACGT	8 cut(s)	505, 665, 778, 1813, 3243, 3334, 3662, 3808
TaqI	TCGA	5 cut(s)	465, 559, 1770, 2196, 2665
TatI	WGTACW	6 cut(s)	145, 862, 1074, 1472, 2357, 3570
TauI	GCSGC	2 cut(s)	702, 754
TfiI	GAWTC	5 cut(s)	2018, 2558, 2721, 3782, 4040
TscAI	CASTG	4 cut(s)	1350, 3139, 3178, 3573
TseFI	GTSAC	8 cut(s)	116, 920, 1207, 1573, 1820, 2207, 2734, 3963
TseI	GCWGC	6 cut(s)	973, 1122, 1541, 2034, 2111, 2425
Tsp45I	GTSAC	8 cut(s)	116, 920, 1207, 1573, 1820, 2207, 2734, 3963
TspGWI	ACGGA	3 cut(s)	341, 1631, 2900
TspRI	CASTG	4 cut(s)	1350, 3139, 3178, 3573
Van91I	CCANNNNNTGG	1 cut(s)	3880
Vha464I	CTTAAG	1 cut(s)	1795
VneI	GTGCAC	1 cut(s)	3564
XceI	RCATGY	4 cut(s)	259, 1612, 3368, 4148
XcmI	CCANNNNNNNNNTGG	2 cut(s)	385, 3877
XmiI	GTMKAC	1 cut(s)	525
XmnI	GAANNNNTTC	3 cut(s)	308, 503, 3333
XspI	CTAG	4 cut(s)	836, 1667, 2337, 2481
ZrmI	AGTACT	1 cut(s)	864
Zsp2I	ATGCAT	3 cut(s)	709, 2634, 2686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.