Rroxscaffold_2G00108020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006967
Physical Location & Seq
Forward (+)
32034090 .. 32037493
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108020.1

Sequence Viewer

Length: 420 bp
ATGGGGCCGGCGCACAAAGGTGGCACCACCCATCCTCCATCGAAGGATCTTTCAGAGGTGGAAATGGACCATTTGGCCCAACAGACGGTGGCTGAAGCCCAACTTCAAGCGGTTCGCTACTCCGTCCACGAGATTCGTGCTTCTCACGAGCGTATGCGTTCTGAGAACGCTGAGATTCAAGCTCAAAACGTTGAATTTCGGTCTCAGTTGCTCGACGAACTTCGAGCTCTTCGGGTTACCTCGGTGACTCATTCTTCACCGTCTTCGGTGTCTCCTAACCCGCTGCAATTTGGCTCTCTTGCATCTTCTAAACCATCTAATTCTACGAGTTCGGCTCCTCCACCTATATACCCGCAAGGTCTGGGTGTTCTGAGCTTCACTTCAGATGTAATTTCTTCAGCGGGAAATCCTGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

14.77

Weight (kDa)

6.03

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 23
AciI CCGC 4 cut(s) 110, 281, 353, 401
AclI AACGTT 1 cut(s) 189
AclWI GGATC 1 cut(s) 54
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 3 cut(s) 114, 366, 381
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 3 cut(s) 107, 179, 194
AleI CACNNNNGTG 1 cut(s) 18
AluBI AGCT 3 cut(s) 182, 227, 375
AluI AGCT 3 cut(s) 182, 227, 375
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 207, 276
AlwI GGATC 1 cut(s) 54
AoxI GGCC 2 cut(s) 5, 75
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 3 cut(s) 5, 67, 76
AsuHPI GGTGA 2 cut(s) 249, 256
AvaII GGWCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 229
BauI CACGAG 2 cut(s) 128, 146
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 1 cut(s) 270
BccI CCATC 3 cut(s) 39, 46, 322
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BcoDI GTCTC 2 cut(s) 207, 276
BfmI CTRYAG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 3 cut(s) 5, 67, 76
BmiI GGNNCC 3 cut(s) 6, 25, 336
BmsI GCATC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 319, 351
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 4 cut(s) 153, 162, 218, 362
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 270
BshFI GGCC 2 cut(s) 7, 77
BshNI GGYRCC 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 2 cut(s) 207, 276
BsnI GGCC 2 cut(s) 7, 77
Bso31I GGTCTC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 4 cut(s) 110, 281, 353, 401
BspANI GGCC 2 cut(s) 7, 77
BspCNI CTCAG 4 cut(s) 154, 163, 217, 363
BspLI GGNNCC 3 cut(s) 6, 25, 336
BspMAI CTGCAG 1 cut(s) 415
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 54
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 46
BssSI CACGAG 2 cut(s) 128, 146
Bst2BI CACGAG 2 cut(s) 128, 146
Bst4CI ACNGT 2 cut(s) 88, 261
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 4 cut(s) 162, 171, 204, 371
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 1 cut(s) 31
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 2 cut(s) 207, 276
BstMBI GATC 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 270
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 2 cut(s) 7, 77
BtsCI GGATG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 5, 67, 76
CviJI RGCY 9 cut(s) 7, 77, 92, 98, 182, 227, 294, 335, 375
CviKI_1 RGCY 9 cut(s) 7, 77, 92, 98, 182, 227, 294, 335, 375
DdeI CTNAG 4 cut(s) 162, 171, 204, 371
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 3 cut(s) 114, 366, 381
Eco91I GGTNACC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 229
FaiI YATR 3 cut(s) 155, 347, 349
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FauI CCCGC 3 cut(s) 288, 360, 394
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 1 cut(s) 18
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 284
GlaI GCGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 284
HaeIII GGCC 2 cut(s) 7, 77
HapII CCGG 1 cut(s) 8
HhaI GCGC 1 cut(s) 13
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HinfI GANTC 3 cut(s) 133, 175, 247
HpaII CCGG 1 cut(s) 8
HphI GGTGA 2 cut(s) 249, 256
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 4 cut(s) 55, 163, 372, 385
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 2 cut(s) 88, 261
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 3 cut(s) 286, 302, 413
HpyF3I CTNAG 4 cut(s) 162, 171, 204, 371
HpySE526I ACGT 1 cut(s) 189
HspAI GCGC 1 cut(s) 11
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Kzo9I GATC 1 cut(s) 46
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 3 cut(s) 21, 347, 399
Lsp1109I GCAGC 1 cut(s) 270
LweI GCATC 1 cut(s) 311
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 235, 244
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 5 cut(s) 221, 246, 255, 297, 387
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 4 cut(s) 194, 287, 319, 390
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 4 cut(s) 45, 49, 250, 348
MroNI GCCGGC 1 cut(s) 7
MseI TTAA 1 cut(s) 418
MslI CAYNNNNRTG 1 cut(s) 18
MspA1I CMGCKG 2 cut(s) 283, 401
MspI CCGG 1 cut(s) 8
NaeI GCCGGC 1 cut(s) 9
NdeII GATC 1 cut(s) 46
NgoMIV GCCGGC 1 cut(s) 7
NlaIV GGNNCC 3 cut(s) 6, 25, 336
NmuCI GTSAC 1 cut(s) 244
OliI CACNNNNGTG 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 133, 175
PkrI GCNGC 1 cut(s) 285
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 229
Psp1406I AACGTT 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 6, 25, 336
PspPI GGNCC 3 cut(s) 5, 67, 76
PstI CTGCAG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 418
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 3 cut(s) 5, 67, 76
SbfI CCTGCAGG 1 cut(s) 415
SchI GAGTC 1 cut(s) 241
SdaI CCTGCAGG 1 cut(s) 415
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 1 cut(s) 311
SfcI CTRYAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 18
Sse8387I CCTGCAGG 1 cut(s) 415
Sse9I AATT 4 cut(s) 194, 287, 319, 390
SsiI CCGC 4 cut(s) 110, 281, 353, 401
SstI GAGCTC 1 cut(s) 229
TaaI ACNGT 2 cut(s) 88, 261
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 41, 213, 223
TaqII GACCGA 1 cut(s) 189
TasI AATT 4 cut(s) 194, 287, 319, 390
TfiI GAWTC 2 cut(s) 133, 175
Tru1I TTAA 1 cut(s) 418
Tru9I TTAA 1 cut(s) 418
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.