Rroxscaffold_2G00131900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006967
Physical Location & Seq
Reverse (-)
68752937 .. 68755469
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131900.1

Sequence Viewer

Length: 558 bp
ATGCCTCGCCTTCAATCTCAGCCAGTCTCGAGTCCAATCCTCCACTGCCAAACCACTGCCGCCACAATAATGGACGGGCACGATTCGGAAGATTCAAAGCATTGCAATGTTGATATGTATCCATTTAATCTCATTCCCTTCCAATCTGAAATTCCGAATTTGAATTCTCTGGAATCACAACCCAATTACCCAAATTTCCAAATTAGGGTTTTGAAAGACGACGGCCACCTCCGGAACAACCCGTATTCCCAGCCACCTCCCAATCGATACGAGCCGAAAGATGACGACGATGCTATCGACGACGATCTGGACGAGGAGAATTACGGCCAAGACAACGTCGTCGTGGAAGAGCAGTACCAGCACAACCTGCACGACCTGAATCTGAGAGTGTATTTGGAGAAGTCGAATGGGTTTGATGAGATGAGGGATATTCCAGGGAGTCCGAGACGGATGAGGAGGAGAATGAAAGTGAGCAGAATGGGTTTGGGTTTGGGATTCCTCCGAGGCAGGGAGGGGGAGGGTTTATTTGCTTGTTTCTTGATTTTGGGGGCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

21.25

Weight (kDa)

4.89

Isoelectric Point (pI)

63.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 231
AciI CCGC 1 cut(s) 60
AcoI YGGCCR 2 cut(s) 223, 325
AcsI RAATTY 4 cut(s) 150, 157, 163, 193
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 2 cut(s) 205, 508
AgsI TTSAA 4 cut(s) 14, 96, 163, 214
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 2 cut(s) 135, 167
Alw26I GTCTC 2 cut(s) 31, 439
Ama87I CYCGRG 1 cut(s) 28
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 2 cut(s) 223, 325
ApoI RAATTY 4 cut(s) 150, 157, 163, 193
AvaI CYCGRG 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 81
BceAI ACGGC 2 cut(s) 238, 340
BciT130I CCWGG 1 cut(s) 435
BciVI GTATCC 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 31, 439
BfuAI ACCTGC 1 cut(s) 375
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 1 cut(s) 280
Bsa29I ATCGAT 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 434, 502
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 508
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 2 cut(s) 100, 112
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 1 cut(s) 435
BseCI ATCGAT 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 434, 502
BseGI GGATG 1 cut(s) 456
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 205, 508
BseMI GCAATG 2 cut(s) 100, 112
BseMII CTCAG 2 cut(s) 32, 374
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 3 cut(s) 329, 469, 472
BseSI GKGCMC 1 cut(s) 81
BseYI CCCAGC 1 cut(s) 249
BsgI GTGCAG 1 cut(s) 353
BshFI GGCC 2 cut(s) 225, 327
BshVI ATCGAT 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 205, 508
BsmAI GTCTC 2 cut(s) 31, 439
BsmBI CGTCTC 1 cut(s) 439
BsnI GGCC 2 cut(s) 225, 327
BsoBI CYCGRG 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 81
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 1 cut(s) 304
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 225, 327
BspCNI CTCAG 2 cut(s) 31, 375
BspDI ATCGAT 1 cut(s) 265
BspEI TCCGGA 1 cut(s) 231
BspMI ACCTGC 1 cut(s) 375
BspQI GCTCTTC 1 cut(s) 342
BsrDI GCAATG 2 cut(s) 100, 112
BsrI ACTGG 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 434, 502
BssMI GATC 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 435
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 18, 383
BstF5I GGATG 1 cut(s) 456
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 2 cut(s) 31, 439
BstMBI GATC 1 cut(s) 304
BstMWI GCNNNNNNNGC 2 cut(s) 358, 367
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 433
BstSLI GKGCMC 1 cut(s) 81
BstXI CCANNNNNNTGG 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 265
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 225, 327
BsuTUI ATCGAT 1 cut(s) 265
BtsCI GGATG 1 cut(s) 456
BtsI GCAGTG 2 cut(s) 43, 54
BtsIMutI CAGTG 2 cut(s) 43, 54
BveI ACCTGC 1 cut(s) 375
ClaI ATCGAT 1 cut(s) 265
Csp6I GTAC 1 cut(s) 355
CviJI RGCY 5 cut(s) 22, 225, 253, 274, 327
CviKI_1 RGCY 5 cut(s) 22, 225, 253, 274, 327
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 18, 383
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 2 cut(s) 223, 325
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 433
Esp3I CGTCTC 1 cut(s) 439
FaiI YATR 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 1 cut(s) 463
Fsp4HI GCNGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 60
GsaI CCCAGC 1 cut(s) 253
HaeIII GGCC 2 cut(s) 225, 327
HapII CCGG 1 cut(s) 232
HinfI GANTC 7 cut(s) 31, 83, 92, 173, 379, 439, 495
HpaII CCGG 1 cut(s) 232
Hpy188I TCNGA 6 cut(s) 88, 148, 156, 384, 444, 503
Hpy188III TCNNGA 5 cut(s) 28, 170, 232, 308, 538
Hpy99I CGWCG 6 cut(s) 224, 290, 302, 305, 341, 344
HpyAV CCTTC 2 cut(s) 20, 148
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 2 cut(s) 105, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 367
HpyF3I CTNAG 2 cut(s) 18, 383
HpySE526I ACGT 1 cut(s) 336
Kpn2I TCCGGA 1 cut(s) 231
Kzo9I GATC 1 cut(s) 304
LguI GCTCTTC 1 cut(s) 342
LweI GCATC 1 cut(s) 280
MaeII ACGT 1 cut(s) 336
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MboII GAAGA 2 cut(s) 101, 359
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 7 cut(s) 150, 157, 163, 184, 193, 201, 319
MlyI GAGTC 2 cut(s) 40, 448
MroI TCCGGA 1 cut(s) 231
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 2 cut(s) 68, 105
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 358, 367
NdeII GATC 1 cut(s) 304
PaeR7I CTCGAG 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 309
PfeI GAWTC 5 cut(s) 83, 92, 173, 379, 495
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 2 cut(s) 39, 447
PpsI GAGTC 2 cut(s) 39, 447
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 335
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 2 cut(s) 68, 105
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 304
SchI GAGTC 2 cut(s) 40, 448
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 81
SetI ASST 5 cut(s) 231, 259, 339, 369, 378
SfaNI GCATC 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 28
SlaI CTCGAG 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 68, 105
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 7 cut(s) 150, 157, 163, 184, 193, 201, 319
SsiI CCGC 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 433
TaiI ACGT 1 cut(s) 339
TaqI TCGA 4 cut(s) 29, 265, 297, 404
TasI AATT 7 cut(s) 150, 157, 163, 184, 193, 201, 319
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 5 cut(s) 83, 92, 173, 379, 495
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 2 cut(s) 50, 61
TspDTI ATGAA 1 cut(s) 479
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 2 cut(s) 50, 61
Tth111I GACNNNGTC 1 cut(s) 335
XapI RAATTY 4 cut(s) 150, 157, 163, 193
XhoI CTCGAG 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.