Rroxscaffold_4G00291450

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005545
Physical Location & Seq
Forward (+)
11892922 .. 11893951
1030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291450.1

Sequence Viewer

Length: 948 bp
ATGAACACCGTTCGGTCCTTCCTTAAGGGAAAACAAAATTGTTATAAATTGAAGGTCTACAAAAAAAAGGTGCGCTACTTGATTCGTGCAGGGTTCTGTTATGGCAACTACGATGGTCTCTCTAGTCCACCAACGTTCGATCTTCATCTGGATGGCAAGAAATGGACAACCGTCAAAACTTCAATGATAGGAGACCCAATATATCGTGAAGCCATATATGAGGCCCGATCAGATCATATTAGCCTGTGCGTTGTCCGAGTTAAGGATGGAGGGGTGCCTTTTATTTCTTCAATAGAGGTTGTTCCATTGGAGGCTCCATTTCCTTTGTACCCTAAGATGCAAAGCAGTCACACTTTCAATCTAGAATCTAGGGTAAACTTGGGTGGGAATGAAGTAATAAGGTACACAGGAATCTTATCAGATGAAAAGTACAACCGGATATGGACTCGTGGAGTAACTCCACCAAACTGCGATGAAGTCACTACCGATTCGGATTCCTCCACAGAAAATGAGCCTCCTGATGAGGTGATCGGCGACTCCATAGAATCACAAAATCTCACTAATCCGATAACCTTGTCCGTTGATATCTCACAACTGCCGACTCCACAATCAGCTTACATTGTGCTCTATTTCGCCGAAAAGACAGTCCTAGAAGCTGGAGACATCAGAATAATTCAAATCTACATTGACGACCATATGAAGTCCACAGTGACACTTGAGTTCAACAAATGCAAGGTGATCACCATATATCCTGTGATTCCTGTTGGTCCTAGGATGAGTGTGACTTTGGCCTCGGACAGTGGTTCCAACCTACCTCCGATGATCAGTGCAATGGAAGTGTTCACAACGTTAGATAAAAGCTCCGCTCCTATCCTCCACTGTCGCTTCTTCTGTGCACTCATTAATTTCGTAATTTCTTGTACCTGTTTGTTTCTGTTTTTAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like

Protein Analysis

315

Amino Acids

35.24

Weight (kDa)

6.0

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 2 - 282 3.7e-55 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 866
AccI GTMKAC 1 cut(s) 57
AciI CCGC 1 cut(s) 864
AclI AACGTT 2 cut(s) 134, 848
AfaI GTAC 4 cut(s) 329, 404, 431, 922
AfiI CCNNNNNNNGG 1 cut(s) 262
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 6 cut(s) 52, 183, 291, 358, 677, 724
AluBI AGCT 3 cut(s) 614, 656, 861
AluI AGCT 3 cut(s) 614, 656, 861
Alw21I GWGCWC 2 cut(s) 627, 898
Alw26I GTCTC 3 cut(s) 122, 186, 654
Alw44I GTGCAC 1 cut(s) 894
AoxI GGCC 2 cut(s) 222, 789
ApaLI GTGCAC 1 cut(s) 894
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
AseI ATTAAT 1 cut(s) 903
AspA2I CCTAGG 1 cut(s) 770
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 15, 223, 767
AsuHPI GGTGA 3 cut(s) 538, 733, 748
AvaII GGWCC 2 cut(s) 15, 767
AvrII CCTAGG 1 cut(s) 770
BaeGI GKGCMC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 274
BauI CACGAG 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 627, 898
BccI CCATC 3 cut(s) 107, 146, 260
BclI TGATCA 2 cut(s) 738, 822
BcoDI GTCTC 3 cut(s) 122, 186, 654
BfaI CTAG 5 cut(s) 123, 362, 369, 650, 771
BfrI CTTAAG 1 cut(s) 23
BlnI CCTAGG 1 cut(s) 770
Bme18I GGWCC 2 cut(s) 15, 767
BmgT120I GGNCC 3 cut(s) 15, 223, 767
BmiI GGNNCC 3 cut(s) 276, 315, 805
BmsI GCATC 1 cut(s) 327
BoxI GACNNNNGTC 1 cut(s) 170
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 737
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 2 cut(s) 122, 186
BsaJI CCNNGG 2 cut(s) 770, 792
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 837
Bse8I GATNNNNATC 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 770, 792
BseGI GGATG 3 cut(s) 157, 271, 780
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 837
BseSI GKGCMC 1 cut(s) 898
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 2 cut(s) 224, 791
BshNI GGYRCC 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 627, 898
BsiSI CCGG 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 122, 186, 654
BsnI GGCC 2 cut(s) 224, 791
Bso31I GGTCTC 2 cut(s) 122, 186
Bsp1286I GDGCHC 2 cut(s) 627, 898
Bsp143I GATC 6 cut(s) 139, 227, 232, 528, 738, 822
BspACI CCGC 1 cut(s) 864
BspANI GGCC 2 cut(s) 224, 791
BspLI GGNNCC 3 cut(s) 276, 315, 805
BspT107I GGYRCC 1 cut(s) 274
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 2 cut(s) 122, 186
BsrBI CCGCTC 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 837
BssECI CCNNGG 2 cut(s) 770, 792
BssMI GATC 6 cut(s) 139, 227, 232, 528, 738, 822
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 770
Bst2BI CACGAG 1 cut(s) 447
Bst4CI ACNGT 6 cut(s) 10, 172, 646, 709, 800, 881
BstAFI CTTAAG 1 cut(s) 23
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 157, 271, 780
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 6 cut(s) 142, 230, 235, 531, 741, 825
BstMAI GTCTC 3 cut(s) 122, 186, 654
BstMBI GATC 6 cut(s) 139, 227, 232, 528, 738, 822
BstPAI GACNNNNGTC 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 898
BsuRI GGCC 2 cut(s) 224, 791
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 3 cut(s) 157, 271, 780
BtsIMutI CAGTG 4 cut(s) 714, 805, 832, 877
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 15, 223, 767
Csp6I GTAC 4 cut(s) 328, 403, 430, 921
CviQI GTAC 4 cut(s) 328, 403, 430, 921
DdeI CTNAG 1 cut(s) 333
DpnI GATC 6 cut(s) 141, 229, 234, 530, 740, 824
DpnII GATC 6 cut(s) 139, 227, 232, 528, 738, 822
Eco130I CCWWGG 1 cut(s) 770
Eco31I GGTCTC 2 cut(s) 122, 186
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 15, 767
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 770
FauNDI CATATG 1 cut(s) 696
FbaI TGATCA 2 cut(s) 738, 822
FblI GTMKAC 1 cut(s) 57
FokI GGATG 3 cut(s) 164, 278, 787
FspBI CTAG 5 cut(s) 123, 362, 369, 650, 771
GlaI GCGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 2 cut(s) 224, 791
HapII CCGG 1 cut(s) 436
HhaI GCGC 1 cut(s) 75
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HpaII CCGG 1 cut(s) 436
HphI GGTGA 3 cut(s) 538, 733, 748
Hpy166II GTNNAC 7 cut(s) 58, 128, 376, 405, 705, 843, 896
Hpy188I TCNGA 8 cut(s) 232, 257, 421, 493, 567, 668, 796, 819
Hpy188III TCNNGA 4 cut(s) 149, 206, 362, 518
Hpy8I GTNNAC 7 cut(s) 58, 128, 376, 405, 705, 843, 896
HpyAV CCTTC 2 cut(s) 28, 46
HpyCH4III ACNGT 6 cut(s) 10, 172, 646, 709, 800, 881
HpyCH4IV ACGT 2 cut(s) 134, 848
HpyCH4V TGCA 5 cut(s) 89, 340, 732, 830, 896
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 134, 848
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 2 cut(s) 738, 822
Kzo9I GATC 6 cut(s) 139, 227, 232, 528, 738, 822
LmnI GCTCC 3 cut(s) 319, 866, 871
LweI GCATC 1 cut(s) 327
MaeI CTAG 5 cut(s) 123, 362, 369, 650, 771
MaeII ACGT 2 cut(s) 134, 848
MaeIII GTNAC 5 cut(s) 347, 454, 478, 709, 781
MalI GATC 6 cut(s) 141, 229, 234, 530, 740, 824
MbiI CCGCTC 1 cut(s) 866
MboI GATC 6 cut(s) 139, 227, 232, 528, 738, 822
MboII GAAGA 3 cut(s) 134, 279, 880
MhlI GDGCHC 2 cut(s) 627, 898
MluCI AATT 5 cut(s) 37, 47, 672, 904, 912
MlyI GAGTC 3 cut(s) 439, 530, 595
MmeI TCCRAC 1 cut(s) 831
MseI TTAA 3 cut(s) 24, 261, 903
MslI CAYNNNNRTG 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 1 cut(s) 436
NdeI CATATG 1 cut(s) 696
NdeII GATC 6 cut(s) 139, 227, 232, 528, 738, 822
NlaIV GGNNCC 3 cut(s) 276, 315, 805
NmuCI GTSAC 4 cut(s) 347, 478, 709, 781
PfeI GAWTC 7 cut(s) 82, 365, 411, 488, 494, 545, 757
PleI GAGTC 3 cut(s) 439, 530, 595
PpsI GAGTC 3 cut(s) 439, 530, 595
PshAI GACNNNNGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 903
PsiI TTATAA 1 cut(s) 45
Psp1406I AACGTT 2 cut(s) 134, 848
PspN4I GGNNCC 3 cut(s) 276, 315, 805
PspPI GGNCC 3 cut(s) 15, 223, 767
RsaI GTAC 4 cut(s) 329, 404, 431, 922
RsaNI GTAC 4 cut(s) 328, 403, 430, 921
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 24, 261, 903
Sau3AI GATC 6 cut(s) 139, 227, 232, 528, 738, 822
Sau96I GGNCC 3 cut(s) 15, 223, 767
SchI GAGTC 3 cut(s) 439, 530, 595
SduI GDGCHC 2 cut(s) 627, 898
SfaNI GCATC 1 cut(s) 327
SinI GGWCC 2 cut(s) 15, 767
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 23, 716
SmoI CTYRAG 2 cut(s) 23, 716
Sse9I AATT 5 cut(s) 37, 47, 672, 904, 912
SsiI CCGC 1 cut(s) 864
SspMI CTAG 5 cut(s) 123, 362, 369, 650, 771
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 6 cut(s) 10, 172, 646, 709, 800, 881
TaiI ACGT 2 cut(s) 137, 851
TaqI TCGA 1 cut(s) 138
TasI AATT 5 cut(s) 37, 47, 672, 904, 912
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 7 cut(s) 82, 365, 411, 488, 494, 545, 757
Tru1I TTAA 3 cut(s) 24, 261, 903
Tru9I TTAA 3 cut(s) 24, 261, 903
TscAI CASTG 4 cut(s) 714, 805, 832, 884
TseFI GTSAC 4 cut(s) 347, 478, 709, 781
Tsp45I GTSAC 4 cut(s) 347, 478, 709, 781
TspDTI ATGAA 6 cut(s) 17, 134, 405, 438, 489, 713
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 4 cut(s) 714, 805, 832, 884
Vha464I CTTAAG 1 cut(s) 23
VneI GTGCAC 1 cut(s) 894
VpaK11BI GGWCC 2 cut(s) 15, 767
VspI ATTAAT 1 cut(s) 903
XbaI TCTAGA 1 cut(s) 361
XmaJI CCTAGG 1 cut(s) 770
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 5 cut(s) 123, 362, 369, 650, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.