Rroxscaffold_5G00333850

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003339
Physical Location & Seq
Forward (+)
1187584 .. 1191081
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333850.1

Sequence Viewer

Length: 489 bp
ATGCTGAAGCGGATTGAGCCGCTTGACAGCTCCGTCATCTCCAAAGGCGTTTCGGATTCCGCTAAGGACTCGATGAAGCAGACCATCTCTACTATGCTGGGATTGCTGCCGTCCGATCAGTTCTCCGTCACCGTTAGGGTTTGCAGAGCTCCCCTTGATCGCCTCCTCGCCTCTTCCATATTCACCGGGTGTACACTGTGGAACGCGGAGTATCGGATATCGTTGATGAGGAATTTTGACATTTCTACAGAGAATTCTGTGGCTTTGGATGGGTCGAAGATCGATGGGAGTTGGGAGACGGGGGATAGTGAGGAAAGGGATAGCGAGAATGGATTTGGGGCGAGTAATCAATACCTGGGCTCAACAAGTCCTCAAGTTTTCGGGGATTTGTCTCCGGAGGCTTTGAATTACATCCAGAGGTTGCAATCAGAGTTAACTAATGTTAAGGAGTATGATAGGTTTATGCTCTCTCTCTCTAGGGGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

162

Amino Acids

17.94

Weight (kDa)

4.69

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 1 - 78 5.5e-19 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AccII CGCG 1 cut(s) 206
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 4 cut(s) 10, 20, 60, 206
AcsI RAATTY 2 cut(s) 232, 253
AcuI CTGAAG 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 189
AfaI GTAC 1 cut(s) 193
AgsI TTSAA 1 cut(s) 406
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 30, 149
AluI AGCT 2 cut(s) 30, 149
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 290, 396
Aor13HI TCCGGA 1 cut(s) 394
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 2 cut(s) 232, 253
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 121, 175
BanII GRGCYC 2 cut(s) 151, 362
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 93
BccI CCATC 3 cut(s) 92, 263, 278
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 2 cut(s) 290, 396
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 246
BisI GCNGC 2 cut(s) 20, 107
BlsI GCNGC 2 cut(s) 21, 108
Bme1390I CCNGG 2 cut(s) 187, 356
BmrFI CCNGG 2 cut(s) 187, 356
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 1 cut(s) 394
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 1 cut(s) 356
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 2 cut(s) 274, 411
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 93
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 2 cut(s) 186, 395
BsmAI GTCTC 2 cut(s) 290, 396
BsmBI CGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 151, 362
Bsp13I TCCGGA 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 115, 157, 279
BspACI CCGC 4 cut(s) 10, 20, 60, 206
BspDI ATCGAT 1 cut(s) 282
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 3 cut(s) 115, 157, 279
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 133, 198
Bst6I CTCTTC 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 274, 411
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 3 cut(s) 118, 160, 282
BstMAI GTCTC 2 cut(s) 290, 396
BstMBI GATC 3 cut(s) 115, 157, 279
BstMWI GCNNNNNNNGC 2 cut(s) 16, 103
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 185, 354
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 282
BsuTUI ATCGAT 1 cut(s) 282
BtsCI GGATG 2 cut(s) 274, 411
BtsIMutI CAGTG 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 282
Csp6I GTAC 1 cut(s) 192
CviJI RGCY 6 cut(s) 19, 30, 149, 263, 360, 401
CviKI_1 RGCY 6 cut(s) 19, 30, 149, 263, 360, 401
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 1 cut(s) 63
DpnI GATC 3 cut(s) 117, 159, 281
DpnII GATC 3 cut(s) 115, 157, 279
DraIII CACNNNGTG 1 cut(s) 189
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 149
Eco24I GRGCYC 2 cut(s) 151, 362
Eco32I GATATC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 219
EcoT38I GRGCYC 2 cut(s) 151, 362
Esp3I CGTCTC 1 cut(s) 290
FaiI YATR 4 cut(s) 95, 179, 453, 464
Fnu4HI GCNGC 2 cut(s) 20, 107
FokI GGATG 2 cut(s) 281, 398
FriOI GRGCYC 2 cut(s) 151, 362
Fsp4HI GCNGC 2 cut(s) 20, 107
FspBI CTAG 1 cut(s) 477
GluI GCNGC 2 cut(s) 20, 107
GsaI CCCAGC 1 cut(s) 101
HapII CCGG 2 cut(s) 186, 395
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 3 cut(s) 56, 68, 483
HpaI GTTAAC 1 cut(s) 435
HpaII CCGG 2 cut(s) 186, 395
HphI GGTGA 2 cut(s) 121, 175
Hpy166II GTNNAC 3 cut(s) 192, 194, 435
Hpy188I TCNGA 4 cut(s) 55, 115, 216, 430
Hpy188III TCNNGA 2 cut(s) 395, 415
Hpy8I GTNNAC 3 cut(s) 192, 194, 435
HpyCH4III ACNGT 2 cut(s) 133, 198
HpyCH4V TGCA 2 cut(s) 144, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 103
HpyF3I CTNAG 1 cut(s) 63
Kpn2I TCCGGA 1 cut(s) 394
KspAI GTTAAC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 115, 157, 279
LmnI GCTCC 2 cut(s) 35, 154
LpnPI CCDG 6 cut(s) 83, 199, 341, 368, 408, 428
Lsp1109I GCAGC 1 cut(s) 93
MaeI CTAG 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 127
MalI GATC 3 cut(s) 117, 159, 281
MboI GATC 3 cut(s) 115, 157, 279
MboII GAAGA 2 cut(s) 165, 289
MhlI GDGCHC 2 cut(s) 151, 362
MluCI AATT 3 cut(s) 232, 253, 406
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 8 cut(s) 173, 176, 181, 222, 304, 381, 391, 411
MroI TCCGGA 1 cut(s) 394
MseI TTAA 2 cut(s) 434, 444
MspI CCGG 2 cut(s) 186, 395
MspR9I CCNGG 2 cut(s) 187, 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 16, 103
NciI CCSGG 1 cut(s) 187
NdeII GATC 3 cut(s) 115, 157, 279
NmuCI GTSAC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 56, 483
PkrI GCNGC 2 cut(s) 21, 108
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 354
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 434, 444
SatI GCNGC 2 cut(s) 20, 107
Sau3AI GATC 3 cut(s) 115, 157, 279
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 187, 356
SduI GDGCHC 2 cut(s) 151, 362
SetI ASST 5 cut(s) 32, 151, 357, 422, 461
SfcI CTRYAG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 372
SmoI CTYRAG 1 cut(s) 372
Sse9I AATT 3 cut(s) 232, 253, 406
SsiI CCGC 4 cut(s) 10, 20, 60, 206
SspMI CTAG 1 cut(s) 477
SstI GAGCTC 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 185, 354
TaaI ACNGT 2 cut(s) 133, 198
TaqI TCGA 3 cut(s) 71, 275, 282
TasI AATT 3 cut(s) 232, 253, 406
TatI WGTACW 1 cut(s) 191
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 2 cut(s) 56, 483
Tru1I TTAA 2 cut(s) 434, 444
Tru9I TTAA 2 cut(s) 434, 444
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 89
TspGWI ACGGA 2 cut(s) 22, 115
TspRI CASTG 1 cut(s) 201
XapI RAATTY 2 cut(s) 232, 253
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.