Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0003870

Physical Location & Seq

Genomic Coordinates Forward (+)

20462098 .. 20464676

Gene Structure 2579 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00181290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGGCGGAACAAGAAGCAGAGACCTCGAGCGGTATCAGAGTCAGTGGCATGCAATTCGCGTACGACGCGCAACCGCCGCTCTTCTACGACTTCAGCCTCAACATTGCTCCCGGATCGCGGTGCCTTCTCGTCGGCGCCAACGGATCCGGGAAGACTACTCTGCTGAAGATTTTGGCGGGGAAGCAAATGGTGGGAGGGAGGGATGTGGTGCGGGTGTTGAATGGTTCGGCTTTTCATGACACTCATTTGGTCTGTAGCGGCGACTTGGCCTATTTGGGAGGCTCTTGGAGCAAAACTGTTGGCTGTGCTGGAGAGCTGCCGCTCCAAGGAGACTTCTCTGCTGAACATATGATATTTGGAGTTGAAGGAACTGATCCAGTTAGGAGAGAAAAGTTGATTGAGCTGCTTGATATAGATCTGAAGTGGCGAATGCATAAAGTATCTGATGGGCAGCGACGCCGAGTGCAAATCTGCATGGGACTTCTTCATCCTTTCAAGGTTCTCTTGCTTGATGAGGTAACAGTCGACTTGGATGTTGTTGCTAGGATGGACTTATTGGATTTCTTTAAGGAAGAATGTGAGCAGAGAGGAGCTACAATTGTATATGCAACTCATATCTTTGATGGCTTGGAACCATGGGCAACCCATTTAGCTTACATCCAAGATGGTGAGCTAAAGAGGGCTCAGAAGTTATTGGAGGTTGAAGAGTTGAAGAGTTCAGTCAATCTGCTTTCTGTTGTCGAGTCCTGGCTTCGTGCTGAAACGAAGCAGGAAAAAAGAAACCCAAATATCCTGCCCAAACGTCATCTCCTTTTGTCTCAGTTCGACTCTGTTTTGATGCTTGTTTCTGCCTTATGTCTGGAACATCATGATCTTGTGCTTTCAAATGGCCTGGAATTATGTACTCTTTTAGGTTTTCCAGATTGCAAAAATAGTCTGCAGAAAATGTTGCCTGTTGCTTCAGTAGTTTCATCTTCTTTTTACGAAAAGATAATGTTGGACCATCTCACGCATTCGATGCCATTTGAATCAAGACTCTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0010114 GO:0010218 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

ABC_tran

Protein Analysis

347

Amino Acids

38.7

Weight (kDa)

5.66

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	30 - 174	4.2e-15	ABC transporter
AAA_21	PF13304	141 - 205	1.2e-06	AAA domain, putative AbiEii toxin, Type IV TA system

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003870)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03905 AT1G03905 AT1G03905 AT1G03905 AT5G44110 AT5G44110 AT5G44110
fragaria_vesca	FvH4_2g21190 FvH4_2g21190
malus_domestica	MD05G1194500.v1.1
prunus_persica	Prupe.8G221200_v2.0.a1
pyrus_communis	pycom05g18180
rosa_chinensis	RchiOBHm_Chr5g0049941 RchiOBHm_Chr6g0287691
rosa_laevigata	RLG00000012473
rosa_multiflora	Rmu_sc0003335.1_g000001 Rmu_sc0003667.1_g000009 Rmu_sc0003877.1_g000018 Rmu_ssc0000100.1_g000025
rosa_roxburghii	Rroxscaffold_5G00344430 Rroxscaffold_7G00181070 Rroxscaffold_7G00181290
rosa_rugosa	Rorug06G0190700
rosa_samantha	Rh3DG342300 Rh6AG301200 Rh6BG306900 Rh6CG302600 Rh6CG315100 Rh6DG298600
rosa_wichuraiana	Rw0G000340 Rw2G014690 Rw2G034360 Rw5G023630 Rw6G025990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	120, 134
AccBSI	CCGCTC	3 cut(s)	30, 79, 322
AccI	GTMKAC	1 cut(s)	525
AccII	CGCG	3 cut(s)	59, 68, 118
AciI	CCGC	9 cut(s)	5, 30, 74, 77, 118, 176, 211, 258, 320
AclWI	GGATC	4 cut(s)	121, 138, 151, 368
AcuI	CTGAAG	4 cut(s)	76, 185, 440, 945
AcyI	GRCGYC	2 cut(s)	135, 457
AfaI	GTAC	2 cut(s)	62, 904
AfiI	CCNNNNNNNGG	2 cut(s)	117, 326
AgsI	TTSAA	7 cut(s)	220, 365, 496, 704, 712, 885, 1028
AjnI	CCWGG	2 cut(s)	746, 891
AluBI	AGCT	5 cut(s)	316, 403, 593, 653, 673
AluI	AGCT	5 cut(s)	316, 403, 593, 653, 673
Alw26I	GTCTC	3 cut(s)	14, 324, 822
AlwI	GGATC	4 cut(s)	121, 138, 151, 368
Ama87I	CYCGRG	1 cut(s)	25
AoxI	GGCC	2 cut(s)	267, 889
ApeKI	GCWGC	3 cut(s)	316, 403, 451
AspLEI	GCGC	2 cut(s)	70, 137
AspS9I	GGNCC	1 cut(s)	1000
AsuC2I	CCSGG	2 cut(s)	111, 148
AsuHPI	GGTGA	1 cut(s)	680
AvaI	CYCGRG	1 cut(s)	25
AvaII	GGWCC	1 cut(s)	1000
BamHI	GGATCC	1 cut(s)	143
BanI	GGYRCC	2 cut(s)	120, 134
BanII	GRGCYC	1 cut(s)	685
BbsI	GAAGAC	1 cut(s)	158
BbvI	GCAGC	3 cut(s)	303, 390, 463
BccI	CCATC	5 cut(s)	440, 541, 617, 659, 1011
BcgI	CGANNNNNNTGC	4 cut(s)	6, 37, 40, 71
BciT130I	CCWGG	2 cut(s)	748, 893
BcnI	CCSGG	2 cut(s)	111, 148
BcoDI	GTCTC	3 cut(s)	14, 324, 822
BfaI	CTAG	1 cut(s)	543
BfmI	CTRYAG	2 cut(s)	253, 938
BfoI	RGCGCY	1 cut(s)	138
BglII	AGATCT	1 cut(s)	415
BisI	GCNGC	6 cut(s)	77, 259, 317, 320, 404, 452
BlsI	GCNGC	6 cut(s)	78, 260, 318, 321, 405, 453
Bme1390I	CCNGG	4 cut(s)	111, 148, 748, 893
Bme18I	GGWCC	1 cut(s)	1000
BmeT110I	CYCGRG	1 cut(s)	25
BmgT120I	GGNCC	1 cut(s)	1000
BmiI	GGNNCC	4 cut(s)	122, 136, 145, 633
BmrFI	CCNGG	4 cut(s)	111, 148, 748, 893
BmsI	GCATC	2 cut(s)	828, 1008
BpiI	GAAGAC	1 cut(s)	158
BpmI	CTGGAG	1 cut(s)	330
BpuMI	CCSGG	2 cut(s)	111, 148
BsaBI	GATNNNNATC	1 cut(s)	414
BsaHI	GRCGYC	2 cut(s)	135, 457
BsaI	GGTCTC	1 cut(s)	14
BsaJI	CCNNGG	2 cut(s)	325, 635
BsaXI	ACNNNNNCTCC	2 cut(s)	792, 822
Bsc4I	CCNNNNNNNGG	2 cut(s)	117, 326
Bse1I	ACTGG	1 cut(s)	377
Bse3DI	GCAATG	1 cut(s)	102
Bse8I	GATNNNNATC	1 cut(s)	414
BseBI	CCWGG	2 cut(s)	748, 893
BseDI	CCNNGG	2 cut(s)	325, 635
BseGI	GGATG	5 cut(s)	208, 487, 538, 552, 657
BseJI	GATNNNNATC	1 cut(s)	414
BseLI	CCNNNNNNNGG	2 cut(s)	117, 326
BseMI	GCAATG	1 cut(s)	102
BseMII	CTCAG	2 cut(s)	698, 833
BseNI	ACTGG	1 cut(s)	377
BseRI	GAGGAG	1 cut(s)	603
BseXI	GCAGC	3 cut(s)	303, 390, 463
Bsh1236I	CGCG	3 cut(s)	59, 68, 118
BshFI	GGCC	2 cut(s)	269, 891
BshNI	GGYRCC	2 cut(s)	120, 134
BsiHKCI	CYCGRG	1 cut(s)	25
BsiSI	CCGG	2 cut(s)	111, 147
BsiWI	CGTACG	1 cut(s)	60
BslFI	GGGAC	1 cut(s)	492
BslI	CCNNNNNNNGG	2 cut(s)	117, 326
BsmAI	GTCTC	3 cut(s)	14, 324, 822
BsmFI	GGGAC	1 cut(s)	492
BsmI	GAATGC	2 cut(s)	435, 1012
BsnI	GGCC	2 cut(s)	269, 891
Bso31I	GGTCTC	1 cut(s)	14
BsoBI	CYCGRG	1 cut(s)	25
Bsp1286I	GDGCHC	1 cut(s)	685
Bsp143I	GATC	5 cut(s)	113, 143, 373, 415, 871
Bsp19I	CCATGG	1 cut(s)	635
BspACI	CCGC	9 cut(s)	5, 30, 74, 77, 118, 176, 211, 258, 320
BspANI	GGCC	2 cut(s)	269, 891
BspCNI	CTCAG	2 cut(s)	697, 832
BspFNI	CGCG	3 cut(s)	59, 68, 118
BspHI	TCATGA	2 cut(s)	235, 868
BspLI	GGNNCC	4 cut(s)	122, 136, 145, 633
BspMAI	CTGCAG	1 cut(s)	942
BspPI	GGATC	4 cut(s)	121, 138, 151, 368
BspQI	GCTCTTC	1 cut(s)	86
BspT107I	GGYRCC	2 cut(s)	120, 134
BspTNI	GGTCTC	1 cut(s)	14
BsrBI	CCGCTC	3 cut(s)	30, 79, 322
BsrDI	GCAATG	1 cut(s)	102
BsrI	ACTGG	1 cut(s)	377
BssECI	CCNNGG	2 cut(s)	325, 635
BssMI	GATC	5 cut(s)	113, 143, 373, 415, 871
BssNI	GRCGYC	2 cut(s)	135, 457
BssT1I	CCWWGG	2 cut(s)	325, 635
Bst2UI	CCWGG	2 cut(s)	748, 893
Bst4CI	ACNGT	2 cut(s)	298, 523
Bst6I	CTCTTC	3 cut(s)	86, 699, 707
BstACI	GRCGYC	2 cut(s)	135, 457
BstAPI	GCANNNNNTGC	1 cut(s)	1018
BstC8I	GCNNGC	1 cut(s)	50
BstDEI	CTNAG	2 cut(s)	684, 819
BstDSI	CCRYGG	1 cut(s)	635
BstF5I	GGATG	5 cut(s)	208, 487, 538, 552, 657
BstFNI	CGCG	3 cut(s)	59, 68, 118
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	2 cut(s)	70, 137
BstKTI	GATC	5 cut(s)	116, 146, 376, 418, 874
BstMAI	GTCTC	3 cut(s)	14, 324, 822
BstMBI	GATC	5 cut(s)	113, 143, 373, 415, 871
BstMWI	GCNNNNNNNGC	4 cut(s)	65, 76, 288, 1018
BstNI	CCWGG	2 cut(s)	748, 893
BstNSI	RCATGY	1 cut(s)	52
BstSCI	CCNGG	4 cut(s)	109, 146, 746, 891
BstSFI	CTRYAG	2 cut(s)	253, 938
BstUI	CGCG	3 cut(s)	59, 68, 118
BstV1I	GCAGC	3 cut(s)	303, 390, 463
BstV2I	GAAGAC	1 cut(s)	158
BstX2I	RGATCY	2 cut(s)	143, 415
BstYI	RGATCY	2 cut(s)	143, 415
BsuRI	GGCC	2 cut(s)	269, 891
BtgI	CCRYGG	1 cut(s)	635
BtsCI	GGATG	5 cut(s)	208, 487, 538, 552, 657
BtsIMutI	CAGTG	1 cut(s)	49
Cac8I	GCNNGC	1 cut(s)	50
CciI	TCATGA	2 cut(s)	235, 868
CfoI	GCGC	2 cut(s)	70, 137
Cfr13I	GGNCC	1 cut(s)	1000
CseI	GACGC	2 cut(s)	74, 465
Csp6I	GTAC	2 cut(s)	61, 903
CviAII	CATG	5 cut(s)	49, 236, 475, 636, 869
CviQI	GTAC	2 cut(s)	61, 903
DdeI	CTNAG	2 cut(s)	684, 819
DinI	GGCGCC	1 cut(s)	136
DpnI	GATC	5 cut(s)	115, 145, 375, 417, 873
DpnII	GATC	5 cut(s)	113, 143, 373, 415, 871
Eam1104I	CTCTTC	3 cut(s)	86, 699, 707
EarI	CTCTTC	3 cut(s)	86, 699, 707
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	2 cut(s)	325, 635
Eco24I	GRGCYC	1 cut(s)	685
Eco31I	GGTCTC	1 cut(s)	14
Eco47I	GGWCC	1 cut(s)	1000
Eco57I	CTGAAG	4 cut(s)	76, 185, 440, 945
Eco88I	CYCGRG	1 cut(s)	25
EcoRII	CCWGG	2 cut(s)	746, 891
EcoT14I	CCWWGG	2 cut(s)	325, 635
EcoT22I	ATGCAT	1 cut(s)	435
EcoT38I	GRGCYC	1 cut(s)	685
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
ErhI	CCWWGG	2 cut(s)	325, 635
FaeI	CATG	5 cut(s)	52, 239, 478, 639, 872
FalI	AAGNNNNNCTT	2 cut(s)	488, 520
FaqI	GGGAC	1 cut(s)	492
FatI	CATG	5 cut(s)	48, 235, 474, 635, 868
FauI	CCCGC	2 cut(s)	169, 204
FauNDI	CATATG	1 cut(s)	348
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	6 cut(s)	77, 259, 317, 320, 404, 452
FokI	GGATG	5 cut(s)	215, 474, 545, 559, 644
FriOI	GRGCYC	1 cut(s)	685
Fsp4HI	GCNGC	6 cut(s)	77, 259, 317, 320, 404, 452
FspBI	CTAG	1 cut(s)	543
GlaI	GCGC	2 cut(s)	69, 136
GluI	GCNGC	6 cut(s)	77, 259, 317, 320, 404, 452
GsuI	CTGGAG	1 cut(s)	330
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	2 cut(s)	269, 891
HapII	CCGG	2 cut(s)	111, 147
HgaI	GACGC	2 cut(s)	74, 465
HhaI	GCGC	2 cut(s)	70, 137
Hin1I	GRCGYC	2 cut(s)	135, 457
Hin1II	CATG	5 cut(s)	52, 239, 478, 639, 872
Hin6I	GCGC	2 cut(s)	68, 135
HinP1I	GCGC	2 cut(s)	68, 135
HincII	GTYRAC	1 cut(s)	526
HindII	GTYRAC	1 cut(s)	526
HinfI	GANTC	5 cut(s)	39, 743, 827, 1028, 1035
HpaII	CCGG	2 cut(s)	111, 147
HphI	GGTGA	1 cut(s)	680
Hpy166II	GTNNAC	1 cut(s)	526
Hpy188I	TCNGA	4 cut(s)	38, 420, 445, 687
Hpy188III	TCNNGA	5 cut(s)	236, 860, 869, 920, 1032
Hpy8I	GTNNAC	1 cut(s)	526
Hpy99I	CGWCG	3 cut(s)	68, 134, 459
HpyAV	CCTTC	2 cut(s)	134, 359
HpyCH4III	ACNGT	2 cut(s)	298, 523
HpyCH4IV	ACGT	1 cut(s)	802
HpyCH4V	TGCA	7 cut(s)	52, 433, 466, 474, 608, 927, 940
HpyF10VI	GCNNNNNNNGC	4 cut(s)	65, 76, 288, 1018
HpyF3I	CTNAG	2 cut(s)	684, 819
HpySE526I	ACGT	1 cut(s)	802
Hsp92I	GRCGYC	2 cut(s)	135, 457
Hsp92II	CATG	5 cut(s)	52, 239, 478, 639, 872
HspAI	GCGC	2 cut(s)	68, 135
KasI	GGCGCC	1 cut(s)	134
Kzo9I	GATC	5 cut(s)	113, 143, 373, 415, 871
LguI	GCTCTTC	1 cut(s)	86
LmnI	GCTCC	4 cut(s)	112, 288, 327, 590
Lsp1109I	GCAGC	3 cut(s)	303, 390, 463
LweI	GCATC	2 cut(s)	828, 1008
MaeI	CTAG	1 cut(s)	543
MaeII	ACGT	1 cut(s)	802
MaeIII	GTNAC	1 cut(s)	517
MalI	GATC	5 cut(s)	115, 145, 375, 417, 873
MbiI	CCGCTC	3 cut(s)	30, 79, 322
MboI	GATC	5 cut(s)	113, 143, 373, 415, 871
MboII	GAAGA	8 cut(s)	73, 163, 178, 476, 584, 716, 724, 966
MfeI	CAATTG	1 cut(s)	597
MflI	RGATCY	2 cut(s)	143, 415
MhlI	GDGCHC	1 cut(s)	685
MluCI	AATT	3 cut(s)	53, 597, 896
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	4 cut(s)	48, 752, 821, 1029
MmeI	TCCRAC	1 cut(s)	978
MnlI	CCTC	9 cut(s)	34, 107, 188, 192, 272, 508, 581, 672, 691
Mph1103I	ATGCAT	1 cut(s)	435
MseI	TTAA	1 cut(s)	567
MspI	CCGG	2 cut(s)	111, 147
MspR9I	CCNGG	4 cut(s)	111, 148, 748, 893
MunI	CAATTG	1 cut(s)	597
Mva1269I	GAATGC	2 cut(s)	435, 1012
MvaI	CCWGG	2 cut(s)	748, 893
MvnI	CGCG	3 cut(s)	59, 68, 118
MwoI	GCNNNNNNNGC	4 cut(s)	65, 76, 288, 1018
NarI	GGCGCC	1 cut(s)	135
NciI	CCSGG	2 cut(s)	111, 148
NcoI	CCATGG	1 cut(s)	635
NdeI	CATATG	1 cut(s)	348
NdeII	GATC	5 cut(s)	113, 143, 373, 415, 871
NlaIII	CATG	5 cut(s)	52, 239, 478, 639, 872
NlaIV	GGNNCC	4 cut(s)	122, 136, 145, 633
NmeAIII	GCCGAG	1 cut(s)	485
NsiI	ATGCAT	1 cut(s)	435
NspI	RCATGY	1 cut(s)	52
PaeI	GCATGC	1 cut(s)	52
PaeR7I	CTCGAG	1 cut(s)	25
PagI	TCATGA	2 cut(s)	235, 868
PciSI	GCTCTTC	1 cut(s)	86
PctI	GAATGC	2 cut(s)	435, 1012
PfeI	GAWTC	1 cut(s)	1028
Pfl23II	CGTACG	1 cut(s)	60
PfoI	TCCNGGA	2 cut(s)	109, 146
PkrI	GCNGC	6 cut(s)	78, 260, 318, 321, 405, 453
PleI	GAGTC	4 cut(s)	47, 751, 821, 1029
PluTI	GGCGCC	1 cut(s)	138
PpsI	GAGTC	4 cut(s)	47, 751, 821, 1029
Psp6I	CCWGG	2 cut(s)	746, 891
PspGI	CCWGG	2 cut(s)	746, 891
PspLI	CGTACG	1 cut(s)	60
PspN4I	GGNNCC	4 cut(s)	122, 136, 145, 633
PspPI	GGNCC	1 cut(s)	1000
PspXI	VCTCGAGB	1 cut(s)	25
PstI	CTGCAG	1 cut(s)	942
PsuI	RGATCY	2 cut(s)	143, 415
RsaI	GTAC	2 cut(s)	62, 904
RsaNI	GTAC	2 cut(s)	61, 903
SalI	GTCGAC	1 cut(s)	524
SapI	GCTCTTC	1 cut(s)	86
SaqAI	TTAA	1 cut(s)	567
SatI	GCNGC	6 cut(s)	77, 259, 317, 320, 404, 452
Sau3AI	GATC	5 cut(s)	113, 143, 373, 415, 871
Sau96I	GGNCC	1 cut(s)	1000
SchI	GAGTC	4 cut(s)	48, 752, 821, 1029
ScrFI	CCNGG	4 cut(s)	111, 148, 748, 893
SduI	GDGCHC	1 cut(s)	685
SfaNI	GCATC	2 cut(s)	828, 1008
SfcI	CTRYAG	2 cut(s)	253, 938
SfoI	GGCGCC	1 cut(s)	136
Sfr274I	CTCGAG	1 cut(s)	25
SinI	GGWCC	1 cut(s)	1000
SlaI	CTCGAG	1 cut(s)	25
SmlI	CTYRAG	1 cut(s)	25
SmoI	CTYRAG	1 cut(s)	25
SphI	GCATGC	1 cut(s)	52
Sse9I	AATT	3 cut(s)	53, 597, 896
SsiI	CCGC	9 cut(s)	5, 30, 74, 77, 118, 176, 211, 258, 320
SspDI	GGCGCC	1 cut(s)	134
SspMI	CTAG	1 cut(s)	543
StyD4I	CCNGG	4 cut(s)	109, 146, 746, 891
StyI	CCWWGG	2 cut(s)	325, 635
TaaI	ACNGT	2 cut(s)	298, 523
TaiI	ACGT	1 cut(s)	805
TaqI	TCGA	5 cut(s)	26, 525, 741, 825, 1016
TasI	AATT	3 cut(s)	53, 597, 896
TatI	WGTACW	1 cut(s)	902
TauI	GCSGC	3 cut(s)	79, 261, 322
TfiI	GAWTC	1 cut(s)	1028
Tru1I	TTAA	1 cut(s)	567
Tru9I	TTAA	1 cut(s)	567
TscAI	CASTG	1 cut(s)	49
TseI	GCWGC	3 cut(s)	316, 403, 451
TspDTI	ATGAA	3 cut(s)	224, 476, 960
TspGWI	ACGGA	1 cut(s)	156
TspRI	CASTG	1 cut(s)	49
VpaK11BI	GGWCC	1 cut(s)	1000
XceI	RCATGY	1 cut(s)	52
XhoI	CTCGAG	1 cut(s)	25
XmiI	GTMKAC	1 cut(s)	525
XspI	CTAG	1 cut(s)	543
Zsp2I	ATGCAT	1 cut(s)	435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.