Rw2G014690

ABC transporter I family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003870
Physical Location & Seq
Reverse (-)
16664746 .. 16668013
3268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014690.1

Sequence Viewer

Length: 636 bp
ATGACTCGTCCTGAAGAAGAAAGAACGAGACGCTTTTATGTCTGTTTTGATTTCAATTTTGATTTCAATTATTTTGATTTATGTCTGTTTGTTTCAACATTGTTGAAATCAGGAAAAGAGGTAGTTGTTTTAATAAATAAAAATTTGTGGGCATTGCTGTTTATTCACTCTGCTGAACATATGATATTTGGAGTGGAAGGAACTGATCCGGTTAGGAGAGAAAAGTTGATTGAGCTGCTTGATATAGATCTCAAGTGGCGAATGCATAAAGTATCTGATGGGCAGCGACGCCGAGTGCAAATCTGCATGGGACTTCTTCATCCTTTCAAGATTCTCTTGCTTGATGAGGTAACAGTCGACTTGGATGTTGTTGCTAGGATGGACTTATTAGATTTCTTCAAGGAAGAATGTGACCAGAGAGGAGCTACAATTGTATACGCAACTCATATCTTTGATGGCTTGGAAACATGGGCAACCCATTTAGCTTACATCCAAGATGGTGAGCTAAAGAGAGCTCAGAAGTTATTGGAGGTTGAAGAGTTGAAGAGTTCACAGGAGAAAAAGAAACACAAAAATCCTGCCCAAACGTCATCTCCTTTTGGTATGTGGTCAAGACACATGGCGTATTACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0010114 GO:0010218 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
ABC_tran

Protein Analysis

211

Amino Acids

24.95

Weight (kDa)

6.45

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_21 PF13304 84 - 149 1.6e-06 AAA domain, putative AbiEii toxin, Type IV TA system
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 357, 435
AciI CCGC 1 cut(s) 631
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 289
AgsI TTSAA 8 cut(s) 55, 67, 96, 106, 328, 400, 536, 544
AluBI AGCT 5 cut(s) 235, 425, 485, 505, 515
AluI AGCT 5 cut(s) 235, 425, 485, 505, 515
Alw21I GWGCWC 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 235, 283
ApoI RAATTY 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 512
BanII GRGCYC 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 517
BbvI GCAGC 2 cut(s) 222, 295
BccI CCATC 4 cut(s) 272, 373, 449, 491
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 1 cut(s) 375
BglII AGATCT 1 cut(s) 247
BisI GCNGC 2 cut(s) 236, 284
BlsI GCNGC 2 cut(s) 237, 285
BpuEI CTTGAG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 289
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 577, 607
Bse3DI GCAATG 1 cut(s) 152
Bse8I GATNNNNATC 1 cut(s) 246
BseGI GGATG 4 cut(s) 319, 370, 384, 489
BseJI GATNNNNATC 1 cut(s) 246
BseMI GCAATG 1 cut(s) 152
BseMII CTCAG 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 2 cut(s) 222, 295
BsiHKAI GWGCWC 1 cut(s) 517
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 22
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 2 cut(s) 205, 247
BspACI CCGC 1 cut(s) 631
BspCNI CTCAG 1 cut(s) 529
BspPI GGATC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 152
BssMI GATC 2 cut(s) 205, 247
BssNAI GTATAC 1 cut(s) 436
BssNI GRCGYC 1 cut(s) 289
Bst1107I GTATAC 1 cut(s) 436
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 2 cut(s) 531, 539
BstACI GRCGYC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 516
BstF5I GGATG 4 cut(s) 319, 370, 384, 489
BstKTI GATC 2 cut(s) 208, 250
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 2 cut(s) 205, 247
BstV1I GCAGC 2 cut(s) 222, 295
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BstZ17I GTATAC 1 cut(s) 436
BtsCI GGATG 4 cut(s) 319, 370, 384, 489
CseI GACGC 2 cut(s) 39, 297
CviAII CATG 3 cut(s) 307, 468, 619
CviJI RGCY 6 cut(s) 235, 425, 459, 485, 505, 515
CviKI_1 RGCY 6 cut(s) 235, 425, 459, 485, 505, 515
DdeI CTNAG 1 cut(s) 516
DpnI GATC 2 cut(s) 207, 249
DpnII GATC 2 cut(s) 205, 247
Eam1104I CTCTTC 2 cut(s) 531, 539
EarI CTCTTC 2 cut(s) 531, 539
Ecl136II GAGCTC 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 515
Eco57I CTGAAG 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 515
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 517
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 3 cut(s) 310, 471, 622
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 324
FatI CATG 3 cut(s) 306, 467, 618
FauNDI CATATG 1 cut(s) 180
FblI GTMKAC 2 cut(s) 357, 435
Fnu4HI GCNGC 2 cut(s) 236, 284
FokI GGATG 4 cut(s) 306, 377, 391, 476
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 2 cut(s) 236, 284
FspBI CTAG 1 cut(s) 375
GluI GCNGC 2 cut(s) 236, 284
HapII CCGG 1 cut(s) 209
HgaI GACGC 2 cut(s) 39, 297
Hin1I GRCGYC 1 cut(s) 289
Hin1II CATG 3 cut(s) 310, 471, 622
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 2 cut(s) 4, 331
HpaII CCGG 1 cut(s) 209
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 3 cut(s) 358, 436, 551
Hpy188I TCNGA 2 cut(s) 277, 519
Hpy188III TCNNGA 4 cut(s) 11, 111, 328, 612
Hpy8I GTNNAC 3 cut(s) 358, 436, 551
Hpy99I CGWCG 1 cut(s) 291
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 587
HpyCH4V TGCA 3 cut(s) 265, 298, 306
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 587
Hsp92I GRCGYC 1 cut(s) 289
Hsp92II CATG 3 cut(s) 310, 471, 622
Kzo9I GATC 2 cut(s) 205, 247
LmnI GCTCC 1 cut(s) 422
LpnPI CCDG 6 cut(s) 24, 96, 222, 428, 539, 591
Lsp1109I GCAGC 2 cut(s) 222, 295
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 587
MaeIII GTNAC 2 cut(s) 349, 410
MalI GATC 2 cut(s) 207, 249
MboI GATC 2 cut(s) 205, 247
MboII GAAGA 7 cut(s) 26, 29, 308, 388, 416, 548, 556
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 4 cut(s) 55, 67, 142, 429
MnlI CCTC 4 cut(s) 112, 340, 413, 523
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 633
MspI CCGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 267
NdeI CATATG 1 cut(s) 180
NdeII GATC 2 cut(s) 205, 247
NlaIII CATG 3 cut(s) 310, 471, 622
NmeAIII GCCGAG 1 cut(s) 317
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 267
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 237, 285
Psp124BI GAGCTC 1 cut(s) 517
PsuI RGATCY 1 cut(s) 247
SacI GAGCTC 1 cut(s) 517
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 131
SatI GCNGC 2 cut(s) 236, 284
Sau3AI GATC 2 cut(s) 205, 247
SduI GDGCHC 1 cut(s) 517
SetI ASST 9 cut(s) 123, 237, 351, 427, 487, 507, 517, 534, 590
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 4 cut(s) 55, 67, 142, 429
SsiI CCGC 1 cut(s) 631
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 517
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 590
TaqI TCGA 1 cut(s) 357
TasI AATT 4 cut(s) 55, 67, 142, 429
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 131
Tru9I TTAA 1 cut(s) 131
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 2 cut(s) 235, 283
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 308
XapI RAATTY 1 cut(s) 142
XmiI GTMKAC 2 cut(s) 357, 435
XspI CTAG 1 cut(s) 375
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.