Rorug01G0175100

Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002779
Physical Location & Seq
Reverse (-)
23832088 .. 23833004
917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0175100.1

Sequence Viewer

Length: 573 bp
ATGTGTGAAAAAGCTGCAACTCTCTCAAATGTGAAATTGGAGCAAGGATCAGTGACAACACTAATCGAGGAAAAGGGCATTGTCAAGGGGATGATTTACAAGAACAAGGCTAGAGAGGAGATGAGAACATATGCTCCACTAACAATTGTGTGCGATGGCTGCCTTTCAAATCTGCGCAAATCTCTCAGTGCACCGAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGATCTTGGAGAACTGTGAGCTTCCACATGCAAATCATGGACATGTCATTTTGGGAGATCCTTCACGTATCTTGTTTTATCCCATCAGTAGCACAGAAGTTCGCTGTTTGGTGGATGTACCTGGAACCAAAGTACCTTCAGTAGCTAATGGTGAAATGGCTAACTATTTGAAAACTATGGTGGCTGCTGAGATTCCCCCACAGCTGCACAATGCTTTTATGGTCACAGTAGAGAAAGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTCCCCACTCCTGGTGCAATTTTATTGGGGGATGCATTCAACATGAGGCATCCTTTAACAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MORN

Protein Analysis

190

Amino Acids

20.56

Weight (kDa)

8.21

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 45 - 190 1.8e-59 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 176
AclWI GGATC 2 cut(s) 55, 278
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 2 cut(s) 345, 360
AfiI CCNNNNNNNGG 2 cut(s) 518, 566
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 4 cut(s) 168, 203, 397, 547
AjnI CCWGG 2 cut(s) 346, 517
AloI GAACNNNNNNTCC 2 cut(s) 118, 150
AluBI AGCT 4 cut(s) 14, 247, 371, 430
AluI AGCT 4 cut(s) 14, 247, 371, 430
Alw21I GWGCWC 1 cut(s) 193
Alw44I GTGCAC 1 cut(s) 189
AlwI GGATC 2 cut(s) 55, 278
ApaLI GTGCAC 1 cut(s) 189
ApeKI GCWGC 5 cut(s) 14, 159, 410, 430, 497
AspLEI GCGC 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 193
BaeI ACNNNNGTAYC 2 cut(s) 342, 375
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 4 cut(s) 146, 397, 417, 484
BccI CCATC 2 cut(s) 149, 317
BciT130I CCWGG 2 cut(s) 348, 519
BfaI CTAG 1 cut(s) 111
BisI GCNGC 5 cut(s) 15, 160, 411, 431, 498
BlsI GCNGC 5 cut(s) 16, 161, 412, 432, 499
Bme1390I CCNGG 2 cut(s) 348, 519
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 2 cut(s) 348, 519
BmsI GCATC 2 cut(s) 529, 565
BsaAI YACGTR 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 518, 566
BseBI CCWGG 2 cut(s) 348, 519
BseGI GGATG 4 cut(s) 96, 346, 544, 556
BseLI CCNNNNNNNGG 2 cut(s) 518, 566
BseMII CTCAG 2 cut(s) 199, 405
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 4 cut(s) 146, 397, 417, 484
BsgI GTGCAG 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 193
BslFI GGGAC 2 cut(s) 192, 494
BslI CCNNNNNNNGG 2 cut(s) 518, 566
BsmFI GGGAC 2 cut(s) 192, 494
BsmI GAATGC 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 3 cut(s) 47, 228, 283
BspCNI CTCAG 2 cut(s) 198, 406
BspLI GGNNCC 1 cut(s) 352
BspPI GGATC 2 cut(s) 55, 278
BssMI GATC 3 cut(s) 47, 228, 283
Bst2UI CCWGG 2 cut(s) 348, 519
Bst4CI ACNGT 2 cut(s) 242, 454
BstBAI YACGTR 1 cut(s) 293
BstDEI CTNAG 2 cut(s) 185, 414
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 4 cut(s) 96, 346, 544, 556
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 3 cut(s) 50, 231, 286
BstMBI GATC 3 cut(s) 47, 228, 283
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 2 cut(s) 348, 519
BstNSI RCATGY 2 cut(s) 257, 272
BstSCI CCNGG 2 cut(s) 346, 517
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 4 cut(s) 146, 397, 417, 484
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 4 cut(s) 96, 346, 544, 556
BtsIMutI CAGTG 2 cut(s) 57, 193
CfoI GCGC 1 cut(s) 177
Csp6I GTAC 2 cut(s) 344, 359
CviAII CATG 5 cut(s) 254, 263, 269, 493, 550
CviJI RGCY 9 cut(s) 14, 110, 159, 247, 371, 386, 410, 430, 497
CviKI_1 RGCY 9 cut(s) 14, 110, 159, 247, 371, 386, 410, 430, 497
CviQI GTAC 2 cut(s) 344, 359
DdeI CTNAG 2 cut(s) 185, 414
DpnI GATC 3 cut(s) 49, 230, 285
DpnII GATC 3 cut(s) 47, 228, 283
Eco57I CTGAAG 1 cut(s) 348
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 346, 517
EcoT22I ATGCAT 1 cut(s) 544
FaeI CATG 5 cut(s) 257, 266, 272, 496, 553
FaiI YATR 9 cut(s) 130, 132, 255, 264, 270, 404, 446, 494, 551
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 2 cut(s) 192, 494
FatI CATG 5 cut(s) 253, 262, 268, 492, 549
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 5 cut(s) 15, 160, 411, 431, 498
FokI GGATG 4 cut(s) 103, 353, 543, 551
Fsp4HI GCNGC 5 cut(s) 15, 160, 411, 431, 498
FspBI CTAG 1 cut(s) 111
FspI TGCGCA 1 cut(s) 176
GlaI GCGC 1 cut(s) 176
GluI GCNGC 5 cut(s) 15, 160, 411, 431, 498
HhaI GCGC 1 cut(s) 177
Hin1II CATG 5 cut(s) 257, 266, 272, 496, 553
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HinfI GANTC 1 cut(s) 418
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 1 cut(s) 473
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 190, 297, 372
HpyCH4III ACNGT 2 cut(s) 242, 454
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 7 cut(s) 17, 191, 257, 433, 481, 524, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 2 cut(s) 185, 414
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 5 cut(s) 257, 266, 272, 496, 553
HspAI GCGC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 47, 228, 283
LmnI GCTCC 2 cut(s) 40, 139
LpnPI CCDG 6 cut(s) 333, 360, 504, 519, 531, 552
Lsp1109I GCAGC 4 cut(s) 146, 397, 417, 484
LweI GCATC 2 cut(s) 529, 565
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 52, 448
MalI GATC 3 cut(s) 49, 230, 285
MboI GATC 3 cut(s) 47, 228, 283
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 3 cut(s) 35, 144, 525
MnlI CCTC 4 cut(s) 61, 109, 220, 546
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 430
MspR9I CCNGG 2 cut(s) 348, 519
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 2 cut(s) 348, 519
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeI CATATG 1 cut(s) 130
NdeII GATC 3 cut(s) 47, 228, 283
NlaIII CATG 5 cut(s) 257, 266, 272, 496, 553
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 52, 448
NsbI TGCGCA 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 2 cut(s) 257, 272
PciI ACATGT 1 cut(s) 268
PctI GAATGC 1 cut(s) 542
PfeI GAWTC 1 cut(s) 418
PkrI GCNGC 5 cut(s) 16, 161, 412, 432, 499
Ppu21I YACGTR 1 cut(s) 293
PscI ACATGT 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 346, 517
PspGI CCWGG 2 cut(s) 346, 517
PspN4I GGNNCC 1 cut(s) 352
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 2 cut(s) 345, 360
RsaNI GTAC 2 cut(s) 344, 359
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 5 cut(s) 15, 160, 411, 431, 498
Sau3AI GATC 3 cut(s) 47, 228, 283
ScrFI CCNGG 2 cut(s) 348, 519
SduI GDGCHC 1 cut(s) 193
SetI ASST 8 cut(s) 16, 201, 249, 295, 349, 364, 373, 432
SfaNI GCATC 2 cut(s) 529, 565
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 3 cut(s) 35, 144, 525
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 346, 517
TaaI ACNGT 2 cut(s) 242, 454
TaiI ACGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 35, 144, 525
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 2 cut(s) 57, 193
TseFI GTSAC 2 cut(s) 52, 448
TseI GCWGC 5 cut(s) 14, 159, 410, 430, 497
Tsp45I GTSAC 2 cut(s) 52, 448
TspRI CASTG 2 cut(s) 57, 193
VneI GTGCAC 1 cut(s) 189
XagI CCTNNNNNAGG 1 cut(s) 564
XceI RCATGY 2 cut(s) 257, 272
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.