Rorug01G0183100

Villin headpiece domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005563
Physical Location & Seq
Forward (+)
25230804 .. 25232267
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0183100.1

Sequence Viewer

Length: 1464 bp
ATGGCCGGAGTCAGAGAGATATTCGTAGTCTCAGCAGCGGGGCAAGGCCACCTGCAACCATGCATGGAGCTCTGCAACCACCTCCTCTCCCGCCACTACCACACCACCATCGTCATTCCCTCCACTCTCTCCTCCGCCATCCCTTCCTCATTTTCCCAAAACCCACTCGCCCAGATCCTCCAGCTCACCGCCTCTTCCGGCCCACCCCGACCCGGACCCGACCCGCTTCGCAACCAAGCCGCCCAGGACCTCGACCGCCACCTCGCCAACCGCCCAGCCACCGCCCCCCGTCCTCTCTGCGCCGTGATCGACTTCCAGATGGGCTGGTCCAAGGAGGTCTTCTGGAAATTCGACGTCCCCGTCGTCGGATTCTTCACCTTCGGCGCGTGCGCCGCCGCCATGGAGTGGGGCTCGTGGAAGGTCCAAGCCGGAGACATCCGCCCCGACGAGATCCGCCCAATTCCCGGCCTACCCGAAGAAATGGCTCTCACTATTTCCGATCTCAAGCGTAAGGCCGGCGGTCCCGGCGGTCCCGGCGGCCCACCACCTGGCGGTCCGAACGGTCCACCTGGAGGTCCGGGCGGTCCGGGTGGCCCACCAGGCGGAGGAGGAAAAGGAGGAGGAGGTCCGCCGAAACCGGGGAACCGGCCGCCGTGGGTCCCGGAGGTGGAAGGCTCGGTAGCGTTGATGTTCAACACTTGTGACTACCTGGAGCGTCCGTTCATCAACTACATGACCGAGCAGATGGGGATTCCGACGTGGGGGGTGGGCCCGCTCTTACCGGAATCATATTGGCAGTCGCCGTCGGATTCTTCAATCCACCACCATCAGATCAGAGCTAAGCAGCGGCAATCGAACTACACCGAAGACGACGTCGTTAAGTGGCTTGACGGAAAGCCTCCCGGGTCGGTGTTGTACGTGGCGTTCGGAAGTGAAGTGGGTCCGACTGCGGAAGAGTATCCGGAGCTGGCTGGCGCATTGGAGGAGTCGACCGGGCCGTTCATCTGGGTCATCCAACCCGGGTCGGGTGGGTCCGACCCAGGCTATGGGTTGGAGAGCAAAGTGGGGGAGAGGGGTTTGATGATACGGGGATGGGCACCACAGCTGTTGATACTGAGCCACCCATCAACGGGTGGGTTCTTGTCACATTGTGGGTGGAACTCAACGGCGGAAGCGTTGGGCCGGGGAGTGCCGTTTTTGGGCTGGCCGGTTAGAGGTGATCAGTACTTCAATGCAAAATTGGTGGTGAACCATTTGAAGGTTGGGTACAGAGTTGCTGAGGACTTGTCGAAGATGGTTAGGAAGGAGGATGTTGTGAGAGGGATAGAGAAGCTGATGGGGGATGAGGAGATGAAGAAGAGGGCGGTGGCGATAGGGCGGAAGTTTGAGAATGGGTTTCCGGCCAGCTCCGAGGCGGCATTGGATGCTTTTGGTGATTTTATTACTAGCCGAAAAGCAACTTAA

Protein Analysis

487

Amino Acids

52.05

Weight (kDa)

6.15

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 304 - 457 4.4e-19 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 60
AasI GACNNNNNNGTC 1 cut(s) 359
AatII GACGTC 2 cut(s) 357, 876
Acc36I ACCTGC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 1096
AccB7I CCANNNNNTGG 3 cut(s) 405, 548, 1046
AccBSI CCGCTC 1 cut(s) 775
AccI GTMKAC 1 cut(s) 989
AccII CGCG 1 cut(s) 386
AccIII TCCGGA 1 cut(s) 961
AclWI GGATC 2 cut(s) 169, 445
AcoI YGGCCR 4 cut(s) 3, 647, 1205, 1401
AcsI RAATTY 1 cut(s) 347
AcyI GRCGYC 2 cut(s) 354, 873
AdeI CACNNNGTG 1 cut(s) 1151
AfaI GTAC 3 cut(s) 917, 1226, 1268
AgsI TTSAA 4 cut(s) 694, 816, 1231, 1258
AjiI CACGTC 1 cut(s) 759
AjnI CCWGG 6 cut(s) 243, 547, 568, 598, 708, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AloI GAACNNNNNNTCC 2 cut(s) 704, 736
AluBI AGCT 7 cut(s) 70, 184, 839, 967, 1105, 1333, 1407
AluI AGCT 7 cut(s) 70, 184, 839, 967, 1105, 1333, 1407
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 34, 426
AlwI GGATC 2 cut(s) 169, 445
Ama87I CYCGRG 2 cut(s) 902, 1019
Aor13HI TCCGGA 1 cut(s) 961
ApaI GGGCCC 1 cut(s) 773
ApeKI GCWGC 2 cut(s) 35, 844
ApoI RAATTY 1 cut(s) 347
ArsI GACNNNNNNTTYG 2 cut(s) 858, 890
AspLEI GCGC 4 cut(s) 302, 386, 392, 977
AsuHPI GGTGA 5 cut(s) 178, 367, 1229, 1258, 1445
AvaI CYCGRG 2 cut(s) 902, 1019
BaeGI GKGCMC 2 cut(s) 773, 1099
BanI GGYRCC 1 cut(s) 1096
BanII GRGCYC 3 cut(s) 72, 413, 773
BarI GAAGNNNNNNTAC 2 cut(s) 1250, 1282
BauI CACGAG 1 cut(s) 412
BbsI GAAGAC 2 cut(s) 331, 873
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 1278
BbvI GCAGC 2 cut(s) 47, 856
BccI CCATC 9 cut(s) 116, 146, 313, 739, 834, 1086, 1132, 1288, 1330
BceAI ACGGC 6 cut(s) 287, 637, 787, 982, 1177, 1182
BciT130I CCWGG 6 cut(s) 245, 549, 570, 600, 710, 1041
BciVI GTATCC 1 cut(s) 969
BclI TGATCA 1 cut(s) 1219
BcoDI GTCTC 2 cut(s) 34, 426
BfaI CTAG 1 cut(s) 1446
BfuAI ACCTGC 1 cut(s) 60
BfuI GTATCC 1 cut(s) 969
BglI GCCNNNNNGGC 1 cut(s) 600
BisI GCNGC 9 cut(s) 36, 240, 393, 396, 538, 650, 845, 848, 1416
BlpI GCTNAGC 1 cut(s) 840
BlsI GCNGC 9 cut(s) 37, 241, 394, 397, 539, 651, 846, 849, 1417
BmcAI AGTACT 1 cut(s) 1226
BmeT110I CYCGRG 2 cut(s) 902, 1019
BmgBI CACGTC 1 cut(s) 759
BmsI GCATC 1 cut(s) 1414
BpiI GAAGAC 2 cut(s) 331, 873
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpmI CTGGAG 3 cut(s) 164, 591, 731
Bpu10I CCTNAGC 1 cut(s) 1278
Bpu1102I GCTNAGC 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 919
BsaHI GRCGYC 2 cut(s) 354, 873
BsaWI WCCGGW 2 cut(s) 781, 961
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 704, 734
Bse118I RCCGGY 3 cut(s) 515, 645, 1207
BseAI TCCGGA 1 cut(s) 961
BseBI CCWGG 6 cut(s) 245, 549, 570, 600, 710, 1041
BseGI GGATG 7 cut(s) 138, 435, 1011, 1097, 1315, 1348, 1429
BseMII CTCAG 3 cut(s) 45, 1106, 1269
BseRI GAGGAG 7 cut(s) 74, 121, 621, 633, 636, 998, 1361
BseSI GKGCMC 2 cut(s) 773, 1099
BseX3I CGGCCG 1 cut(s) 647
BseXI GCAGC 2 cut(s) 47, 856
BseYI CCCAGC 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 386
Bsh1285I CGRYCG 3 cut(s) 256, 650, 993
BshNI GGYRCC 1 cut(s) 1096
BsiEI CGRYCG 3 cut(s) 256, 650, 993
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 2 cut(s) 902, 1019
BslFI GGGAC 4 cut(s) 341, 507, 516, 644
BsmAI GTCTC 2 cut(s) 34, 426
BsmFI GGGAC 4 cut(s) 341, 507, 516, 644
BsoBI CYCGRG 2 cut(s) 902, 1019
Bsp120I GGGCCC 1 cut(s) 769
Bsp1286I GDGCHC 4 cut(s) 72, 413, 773, 1099
Bsp13I TCCGGA 1 cut(s) 961
Bsp143I GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
Bsp1720I GCTNAGC 1 cut(s) 840
Bsp19I CCATGG 1 cut(s) 399
BspCNI CTCAG 3 cut(s) 44, 1107, 1270
BspEI TCCGGA 1 cut(s) 961
BspFNI CGCG 1 cut(s) 386
BspMI ACCTGC 1 cut(s) 60
BspPI GGATC 2 cut(s) 169, 445
BspT107I GGYRCC 1 cut(s) 1096
BsrBI CCGCTC 1 cut(s) 775
BsrFI RCCGGY 3 cut(s) 515, 645, 1207
BssAI RCCGGY 3 cut(s) 515, 645, 1207
BssMI GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
BssNI GRCGYC 2 cut(s) 354, 873
BssSI CACGAG 1 cut(s) 412
BssT1I CCWWGG 2 cut(s) 330, 399
Bst2BI CACGAG 1 cut(s) 412
Bst2UI CCWGG 6 cut(s) 245, 549, 570, 600, 710, 1041
Bst4CI ACNGT 1 cut(s) 563
Bst6I CTCTTC 3 cut(s) 199, 948, 1352
BstACI GRCGYC 2 cut(s) 354, 873
BstAPI GCANNNNNTGC 1 cut(s) 1424
BstBAI YACGTR 1 cut(s) 919
BstC8I GCNNGC 7 cut(s) 388, 517, 773, 969, 973, 1205, 1405
BstDEI CTNAG 4 cut(s) 31, 840, 1115, 1278
BstDSI CCRYGG 2 cut(s) 399, 653
BstF5I GGATG 7 cut(s) 138, 435, 1011, 1097, 1315, 1348, 1429
BstFNI CGCG 1 cut(s) 386
BstHHI GCGC 4 cut(s) 302, 386, 392, 977
BstKTI GATC 6 cut(s) 177, 309, 453, 502, 834, 1222
BstMAI GTCTC 2 cut(s) 34, 426
BstMBI GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
BstMCI CGRYCG 3 cut(s) 256, 650, 993
BstMWI GCNNNNNNNGC 5 cut(s) 392, 525, 534, 600, 1424
BstNI CCWGG 6 cut(s) 245, 549, 570, 600, 710, 1041
BstSLI GKGCMC 2 cut(s) 773, 1099
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 2 cut(s) 47, 856
BstV2I GAAGAC 2 cut(s) 331, 873
BstX2I RGATCY 2 cut(s) 174, 450
BstYI RGATCY 2 cut(s) 174, 450
BstZI CGGCCG 1 cut(s) 647
BsuI GTATCC 1 cut(s) 969
BtgI CCRYGG 2 cut(s) 399, 653
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 7 cut(s) 138, 435, 1011, 1097, 1315, 1348, 1429
BveI ACCTGC 1 cut(s) 60
Cac8I GCNNGC 7 cut(s) 388, 517, 773, 969, 973, 1205, 1405
CfoI GCGC 4 cut(s) 302, 386, 392, 977
Cfr10I RCCGGY 3 cut(s) 515, 645, 1207
Cfr9I CCCGGG 2 cut(s) 902, 1019
CpoI CGGWCCG 2 cut(s) 554, 584
CseI GACGC 1 cut(s) 704
Csp6I GTAC 3 cut(s) 916, 1225, 1267
CspCI CAANNNNNGTGG 4 cut(s) 1089, 1124, 1224, 1259
CspI CGGWCCG 2 cut(s) 554, 584
CviAII CATG 4 cut(s) 60, 64, 400, 733
CviQI GTAC 3 cut(s) 916, 1225, 1267
DdeI CTNAG 4 cut(s) 31, 840, 1115, 1278
DpnI GATC 6 cut(s) 176, 308, 452, 501, 833, 1221
DpnII GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
DraIII CACNNNGTG 1 cut(s) 1151
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 4 cut(s) 3, 647, 1205, 1401
EagI CGGCCG 1 cut(s) 647
Eam1104I CTCTTC 3 cut(s) 199, 948, 1352
EarI CTCTTC 3 cut(s) 199, 948, 1352
EciI GGCGGA 7 cut(s) 124, 428, 443, 618, 618, 1184, 1393
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 647
Eco130I CCWWGG 2 cut(s) 330, 399
Eco24I GRGCYC 3 cut(s) 72, 413, 773
Eco52I CGGCCG 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 902, 1019
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 2 cut(s) 247, 658
EcoRII CCWGG 6 cut(s) 243, 547, 568, 598, 708, 1039
EcoT14I CCWWGG 2 cut(s) 330, 399
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 3 cut(s) 72, 413, 773
ErhI CCWWGG 2 cut(s) 330, 399
FaeI CATG 4 cut(s) 63, 67, 403, 736
FaiI YATR 6 cut(s) 61, 65, 401, 734, 790, 1047
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 4 cut(s) 341, 507, 516, 644
FatI CATG 4 cut(s) 59, 63, 399, 732
FauI CCCGC 4 cut(s) 31, 98, 231, 780
FbaI TGATCA 1 cut(s) 1219
FblI GTMKAC 1 cut(s) 989
Fnu4HI GCNGC 9 cut(s) 36, 240, 393, 396, 538, 650, 845, 848, 1416
FokI GGATG 7 cut(s) 125, 422, 998, 1104, 1322, 1355, 1436
FriOI GRGCYC 3 cut(s) 72, 413, 773
Fsp4HI GCNGC 9 cut(s) 36, 240, 393, 396, 538, 650, 845, 848, 1416
FspBI CTAG 1 cut(s) 1446
GlaI GCGC 4 cut(s) 301, 385, 391, 976
GluI GCNGC 9 cut(s) 36, 240, 393, 396, 538, 650, 845, 848, 1416
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 3 cut(s) 164, 591, 731
HgaI GACGC 1 cut(s) 704
HhaI GCGC 4 cut(s) 302, 386, 392, 977
Hin1I GRCGYC 2 cut(s) 354, 873
Hin1II CATG 4 cut(s) 63, 67, 403, 736
Hin6I GCGC 4 cut(s) 300, 384, 390, 975
HinP1I GCGC 4 cut(s) 300, 384, 390, 975
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HinfI GANTC 6 cut(s) 9, 369, 751, 785, 809, 986
HphI GGTGA 5 cut(s) 178, 367, 1229, 1258, 1445
Hpy166II GTNNAC 3 cut(s) 566, 990, 1249
Hpy188III TCNNGA 3 cut(s) 316, 343, 962
Hpy8I GTNNAC 3 cut(s) 566, 990, 1249
Hpy99I CGWCG 8 cut(s) 356, 365, 368, 449, 760, 808, 875, 878
HpyAV CCTTC 6 cut(s) 153, 388, 412, 665, 1252, 1297
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4IV ACGT 4 cut(s) 354, 758, 873, 918
HpyCH4V TGCA 4 cut(s) 55, 63, 75, 1235
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 525, 534, 600, 1424
HpyF3I CTNAG 4 cut(s) 31, 840, 1115, 1278
HpySE526I ACGT 4 cut(s) 354, 758, 873, 918
Hsp92I GRCGYC 2 cut(s) 354, 873
Hsp92II CATG 4 cut(s) 63, 67, 403, 736
HspAI GCGC 4 cut(s) 300, 384, 390, 975
KflI GGGWCCC 1 cut(s) 658
Kpn2I TCCGGA 1 cut(s) 961
KroI GCCGGC 1 cut(s) 515
KroNI GCCGGC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 1219
Kzo9I GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
LmnI GCTCC 4 cut(s) 67, 712, 964, 1412
Lsp1109I GCAGC 2 cut(s) 47, 856
LweI GCATC 1 cut(s) 1414
MaeI CTAG 1 cut(s) 1446
MaeII ACGT 4 cut(s) 354, 758, 873, 918
MaeIII GTNAC 2 cut(s) 701, 1143
MalI GATC 6 cut(s) 176, 308, 452, 501, 833, 1221
MbiI CCGCTC 1 cut(s) 775
MboI GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
MflI RGATCY 2 cut(s) 174, 450
MhlI GDGCHC 4 cut(s) 72, 413, 773, 1099
MluCI AATT 3 cut(s) 347, 459, 1238
MlyI GAGTC 2 cut(s) 18, 995
MmeI TCCRAC 7 cut(s) 346, 779, 786, 968, 1032, 1039, 1059
Mph1103I ATGCAT 1 cut(s) 65
MroI TCCGGA 1 cut(s) 961
MroNI GCCGGC 1 cut(s) 515
MseI TTAA 2 cut(s) 879, 1462
MspA1I CMGCKG 3 cut(s) 38, 847, 1105
MvaI CCWGG 6 cut(s) 245, 549, 570, 600, 710, 1041
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 5 cut(s) 392, 525, 534, 600, 1424
NaeI GCCGGC 1 cut(s) 517
NcoI CCATGG 1 cut(s) 399
NdeII GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
NgoMIV GCCGGC 1 cut(s) 515
NlaIII CATG 4 cut(s) 63, 67, 403, 736
NmuCI GTSAC 2 cut(s) 701, 1143
NsiI ATGCAT 1 cut(s) 65
PaqCI CACCTGC 1 cut(s) 60
PcsI WCGNNNNNNNCGW 4 cut(s) 357, 360, 995, 1172
PdiI GCCGGC 1 cut(s) 517
PfeI GAWTC 4 cut(s) 369, 751, 785, 809
PflFI GACNNNGTC 1 cut(s) 872
PflMI CCANNNNNTGG 3 cut(s) 405, 548, 1046
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 9 cut(s) 37, 241, 394, 397, 539, 651, 846, 849, 1417
PleI GAGTC 2 cut(s) 17, 994
PpsI GAGTC 2 cut(s) 17, 994
Ppu21I YACGTR 1 cut(s) 919
PpuMI RGGWCCY 2 cut(s) 247, 658
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 2 cut(s) 247, 658
Psp6I CCWGG 6 cut(s) 243, 547, 568, 598, 708, 1039
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 6 cut(s) 243, 547, 568, 598, 708, 1039
PspOMI GGGCCC 1 cut(s) 769
PspPPI RGGWCCY 2 cut(s) 247, 658
PsrI GAACNNNNNNTAC 2 cut(s) 908, 940
PsuI RGATCY 2 cut(s) 174, 450
PsyI GACNNNGTC 1 cut(s) 872
PvuII CAGCTG 1 cut(s) 1105
RsaI GTAC 3 cut(s) 917, 1226, 1268
RsaNI GTAC 3 cut(s) 916, 1225, 1267
Rsr2I CGGWCCG 2 cut(s) 554, 584
RsrII CGGWCCG 2 cut(s) 554, 584
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 988
SaqAI TTAA 2 cut(s) 879, 1462
SatI GCNGC 9 cut(s) 36, 240, 393, 396, 538, 650, 845, 848, 1416
Sau3AI GATC 6 cut(s) 174, 306, 450, 499, 831, 1219
ScaI AGTACT 1 cut(s) 1226
SchI GAGTC 2 cut(s) 18, 995
SduI GDGCHC 4 cut(s) 72, 413, 773, 1099
SfaNI GCATC 1 cut(s) 1414
SmaI CCCGGG 2 cut(s) 904, 1021
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 3 cut(s) 347, 459, 1238
SspMI CTAG 1 cut(s) 1446
SstI GAGCTC 1 cut(s) 72
StyI CCWWGG 2 cut(s) 330, 399
TaaI ACNGT 1 cut(s) 563
TaiI ACGT 4 cut(s) 357, 761, 876, 921
TaqI TCGA 6 cut(s) 252, 309, 351, 854, 989, 1289
TaqII GACCGA 1 cut(s) 752
TasI AATT 3 cut(s) 347, 459, 1238
TatI WGTACW 1 cut(s) 1224
TauI GCSGC 7 cut(s) 242, 395, 398, 540, 652, 850, 1418
TfiI GAWTC 4 cut(s) 369, 751, 785, 809
Tru1I TTAA 2 cut(s) 879, 1462
Tru9I TTAA 2 cut(s) 879, 1462
TseFI GTSAC 2 cut(s) 701, 1143
TseI GCWGC 2 cut(s) 35, 844
Tsp45I GTSAC 2 cut(s) 701, 1143
TspDTI ATGAA 3 cut(s) 712, 991, 1367
TspGWI ACGGA 2 cut(s) 708, 906
TspMI CCCGGG 2 cut(s) 902, 1019
Tth111I GACNNNGTC 1 cut(s) 872
Van91I CCANNNNNTGG 3 cut(s) 405, 548, 1046
XapI RAATTY 1 cut(s) 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 1259
XmaI CCCGGG 2 cut(s) 902, 1019
XmiI GTMKAC 1 cut(s) 989
XspI CTAG 1 cut(s) 1446
ZraI GACGTC 2 cut(s) 355, 874
ZrmI AGTACT 1 cut(s) 1226
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.