Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0005563

Physical Location & Seq

Genomic Coordinates Forward (+)

25239490 .. 25245112

Gene Structure 5623 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0183200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGGCGTTGCGACGTCTCTCATTGGTGGCAGTCCTACTCCTGTTCTCGGCGGCTGTTACTACTACGACAACAGCAGCTGCTGCTCAAGCCAAGTCTGGCTGCACAGACAAGTGCGGCAATCTCACAATTCCATATCCATTTGGCATGAAAGAGGGTTGTTACCTGCGAGAAGAATTCTTTATCAATTGCAGCCATACGACCCTATCACCAAAGGCATATTTGACGAATCTCATTGTTACCAACATTTCCCTTGATGACGGTGAGTTGCAAATATTGGGCAACGGAGCTGAATATTGCTATGACGCCCAGGGCAATGTGACGTCCGAACTTTATTCTGTGCTCACCCTGTCTCTTCCTTACACGATCTCCCATTCCAAAAACAAGTTTTATGCCGTTGGCTGTGACAATATTTCTTCACTTTCTCAAGGCTTCCGAGGGGAAGATGAGCTCACTTTCGGGTGCCGGTCAGTATGCAACAGGCCTGGCATTGGTGATCAAAACTCTTGCTCTAGCAGTGGGTGTTGCGAGACTAGAATACCCCGCGGACTGAAAAATTGGACGCTGTATTTGACTCCCTTGGACAGTCATTCTGATATATGGAAGTTTAATCGCTGCAGATATTCCTTCATTGCGAAACAAGGCGAATTAAATTTCTCTGCTGCAATTTTAAAGATACCGAACTACAACTTGCTTCCGGTGGTTCTTAATTTTGCTATTGGGAATGACAAAGACTCCTGTGATGAAGCTCAAAAGAGGAAGGATTTTGCATGTAAGGCTAATAGCATTTGCTTCAACCGGCGAATCGTTGGGTCTGGGGGTTACTTGTGCCGGTGCTTGCCTGGTTATGAAGGAAACCCTTACCACCCAGGTGGTTGCCAAGATATCAATGAGTGCAGCACTTTAAACCCTTGCCAGTTTGGAGAGTGCTTAAATTCACCTCCAGGAAGTTACTCTTGTTTATGTCACAAAGGATACAAAAACGACCGCATGAATGACAAGATTTGCATAAAACACAATCATTCTAAAAGACACTCAAAGATCGTTCTCCTGCGTATTGTTTTATTTGGTACTGGCAGCAGTCTTGGACTAATACTTCTAGCCATAGCTGCATGGTGGTTGGTCCAAGTCATAAAGAAAAGGAAAAACAAAAAACGAAAGGAGAAGTTTTTCAAACAAAATGGTGGTTTAGTATTGGAGCAACAATTATCTTCTGGTGATCAAGCTAATGTTGAAAAAATTAAGTTGTTCAATTCAAGGGAGCTGGAGAAAGCCACAGACCAGTTTAGCATAGATAGAATTCTTGGCCAGGGTGGCCAAGGTACTGTTTACAGTGGAATGTTGATGGATGGACAAACTGTTGCTGTCAAAAAGTCTAAAATAGTTAACGGAGGCGAAGTTCAACAGTTCATTAATGAAATTGTGATTCTTTCACAAATTAACCACAGAAATGTGGTTAAACTATTGGGTTGTTGTTTGGAGACCGAAATTCCCCTCTTGGTCTATGAATTTCTACCAAACGGAACACTTTCTCAGTACATTCATGACCCAGATGAAGAGTTTCCTCTTACATGGGGAACGCGATTGCGAGTGTCTACAGAAGTTGCTGGAGCTCTTTCATATTTACACTCGGCAGCTTCTATTCCTATTTACCACCTGGACATCAAGTCCACAAACATACTCTTGGATGCCGAGTATAGAGCAAAAATTGCAGATTTTGGGACTTCCAGATCCATTTCCATTGACCAGACACACCTCACCACACTGATACATGGAACATTTGGTTATTTGGACCCAGAGTACTTTCAATCAAGCCAATTTACAGAAAAGAGTGATGTTTACAGCTTCGGTGTGGTCCTTGCTGAGCTCTTGACCGGACAAAAACCAGTTTTCATGATGAGGTCGCAAGAAAGTAGAAGCCTAGCAACTCATTTTCTCCTTTCCATGGAGCAGAATCGTTTGTTTGATATTCTTGACGCTCAAGTTATGAAAGATGGTGGCAAAGAAGATATTATAGCAGTTGCTAACCTTGCACAAAGTTGCTTAAACTTGGATGGAAGGAAACGTCCTACTATGAAAGAAGTAGCGGCGGAATTAGAGGGAGTTCGATTGGCAGTCAAAGATGTTGATCATGTTCAACAAAATTTGGTAGAGGAAGGAGATGTCGGAACTCATGAAATAACCACCGAGACTTATTGCGATGTTGTTTCGACATCACCCGGTCCTTTTACAGATGGTGGTACTGCAGGTTCTTCATCTGATGTACAACCACTAATGTTTTATAATACACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046982 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPR_1 TPR_11 TPR_2 TPR_8

Protein Analysis

763

Amino Acids

84.31

Weight (kDa)

6.51

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	34 - 91	1.8e-09	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	429 - 695	9e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	430 - 698	5.2e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005563)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr5g0071761
rosa_multiflora	Rmu_sc0000694.1_g000024 Rmu_sc0002285.1_g000031 Rmu_sc0005500.1_g000002 Rmu_sc0013612.1_g000018
rosa_roxburghii	Rroxscaffold_4G00322960 Rroxscaffold_5G00335950 Rroxscaffold_5G00366950 Rroxscaffold_6G00388330 Rroxscaffold_6G00389350 Rroxscaffold_7G00165060 Rroxscaffold_7G00197390
rosa_rugosa	Rorug01G0183100 Rorug01G0183200 Rorug03G0077400 Rorug05G0261600 Rorug05G0261700
rosa_samantha	Rh2AG454500 Rh2BG458700 Rh2CG441600 Rh4CG103500 Rh4CG324600 Rh4DG275900 Rh4DG307600 Rh5CG264700 Rh6CG064500 Rh6CG148200 Rh6DG061500 Rh7CG217000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2280
AatII	GACGTC	2 cut(s)	16, 323
Acc36I	ACCTGC	2 cut(s)	171, 2234
AccB1I	GGYRCC	1 cut(s)	459
AccI	GTMKAC	1 cut(s)	1592
AccII	CGCG	2 cut(s)	543, 1579
AciI	CCGC	7 cut(s)	50, 114, 541, 543, 985, 2084, 2087
AclWI	GGATC	1 cut(s)	1722
AcoI	YGGCCR	2 cut(s)	1303, 1312
AcsI	RAATTY	7 cut(s)	173, 649, 931, 1296, 1485, 1505, 2140
AcyI	GRCGYC	3 cut(s)	13, 303, 320
AfaI	GTAC	6 cut(s)	1069, 1321, 1535, 1799, 2239, 2262
AfiI	CCNNNNNNNGG	3 cut(s)	46, 488, 1942
AgsI	TTSAA	8 cut(s)	793, 1171, 1232, 1249, 1254, 1400, 1805, 2135
AhdI	GACNNNNNGTC	1 cut(s)	1663
AjnI	CCWGG	7 cut(s)	306, 481, 838, 865, 940, 1305, 1653
AleI	CACNNNNGTG	1 cut(s)	867
AloI	GAACNNNNNNTCC	2 cut(s)	1380, 1412
Alw21I	GWGCWC	4 cut(s)	342, 450, 1612, 1866
Alw26I	GTCTC	5 cut(s)	20, 354, 521, 1472, 2180
AlwI	GGATC	1 cut(s)	1722
AlwNI	CAGNNNCTG	2 cut(s)	77, 80
AoxI	GGCC	3 cut(s)	479, 1303, 1312
ApoI	RAATTY	7 cut(s)	173, 649, 931, 1296, 1485, 1505, 2140
ArsI	GACNNNNNNTTYG	2 cut(s)	550, 582
AseI	ATTAAT	1 cut(s)	1410
Asp700I	GAANNNNTTC	3 cut(s)	1166, 1525, 1557
AspS9I	GGNCC	4 cut(s)	1120, 1789, 1852, 2219
AsuC2I	CCSGG	1 cut(s)	2217
AsuHPI	GGTGA	8 cut(s)	198, 272, 334, 503, 927, 1226, 1747, 2205
AvaII	GGWCC	4 cut(s)	1120, 1789, 1852, 2219
BalI	TGGCCA	2 cut(s)	1305, 1314
BanI	GGYRCC	1 cut(s)	459
BanII	GRGCYC	3 cut(s)	450, 1612, 1866
Bbv12I	GWGCWC	4 cut(s)	342, 450, 1612, 1866
BccI	CCATC	5 cut(s)	1336, 1340, 1985, 2045, 2225
BceAI	ACGGC	1 cut(s)	377
BciT130I	CCWGG	7 cut(s)	308, 483, 840, 867, 942, 1307, 1655
BciVI	GTATCC	1 cut(s)	965
BclI	TGATCA	3 cut(s)	493, 1216, 2125
BcnI	CCSGG	1 cut(s)	2217
BcoDI	GTCTC	5 cut(s)	20, 354, 521, 1472, 2180
BfaI	CTAG	4 cut(s)	510, 531, 1097, 1919
BfmI	CTRYAG	3 cut(s)	613, 1593, 2241
BfuAI	ACCTGC	2 cut(s)	171, 2234
BfuI	GTATCC	1 cut(s)	965
BglI	GCCNNNNNGGC	1 cut(s)	1311
BlpI	GCTNAGC	1 cut(s)	1860
BmcAI	AGTACT	1 cut(s)	1799
Bme1390I	CCNGG	8 cut(s)	308, 483, 840, 867, 942, 1307, 1655, 2217
Bme18I	GGWCC	4 cut(s)	1120, 1789, 1852, 2219
BmeRI	GACNNNNNGTC	1 cut(s)	1663
BmgT120I	GGNCC	4 cut(s)	1120, 1789, 1852, 2219
BmiI	GGNNCC	2 cut(s)	461, 1791
BmrFI	CCNGG	8 cut(s)	308, 483, 840, 867, 942, 1307, 1655, 2217
BmsI	GCATC	1 cut(s)	1675
BpmI	CTGGAG	3 cut(s)	924, 1283, 1626
Bpu1102I	GCTNAGC	1 cut(s)	1860
BpuEI	CTTGAG	3 cut(s)	69, 408, 1962
BpuMI	CCSGG	1 cut(s)	2217
BsaBI	GATNNNNATC	1 cut(s)	2124
BsaHI	GRCGYC	3 cut(s)	13, 303, 320
BsaI	GGTCTC	1 cut(s)	1472
BsaJI	CCNNGG	9 cut(s)	306, 307, 433, 541, 576, 865, 1306, 1315, 1941
BsaWI	WCCGGW	2 cut(s)	694, 1871
BsaXI	ACNNNNNCTCC	6 cut(s)	350, 380, 716, 746, 1380, 1410
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 488, 1942
Bse118I	RCCGGY	3 cut(s)	462, 795, 828
Bse1I	ACTGG	4 cut(s)	913, 1075, 1279, 1883
Bse3DI	GCAATG	2 cut(s)	319, 627
Bse8I	GATNNNNATC	1 cut(s)	2124
BseBI	CCWGG	7 cut(s)	308, 483, 840, 867, 942, 1307, 1655
BseDI	CCNNGG	9 cut(s)	306, 307, 433, 541, 576, 865, 1306, 1315, 1941
BseGI	GGATG	3 cut(s)	1351, 1690, 2056
BseJI	GATNNNNATC	1 cut(s)	2124
BseLI	CCNNNNNNNGG	3 cut(s)	46, 488, 1942
BseMI	GCAATG	2 cut(s)	319, 627
BseMII	CTCAG	2 cut(s)	1544, 1851
BseNI	ACTGG	4 cut(s)	913, 1075, 1279, 1883
BsgI	GTGCAG	2 cut(s)	85, 913
Bsh1236I	CGCG	2 cut(s)	543, 1579
Bsh1285I	CGRYCG	1 cut(s)	985
BshFI	GGCC	3 cut(s)	481, 1305, 1314
BshNI	GGYRCC	1 cut(s)	459
BsiEI	CGRYCG	1 cut(s)	985
BsiHKAI	GWGCWC	4 cut(s)	342, 450, 1612, 1866
BsiSI	CCGG	6 cut(s)	463, 695, 796, 829, 1872, 2217
BslFI	GGGAC	1 cut(s)	1732
BslI	CCNNNNNNNGG	3 cut(s)	46, 488, 1942
BsmAI	GTCTC	5 cut(s)	20, 354, 521, 1472, 2180
BsmBI	CGTCTC	1 cut(s)	20
BsmFI	GGGAC	1 cut(s)	1732
BsnI	GGCC	3 cut(s)	481, 1305, 1314
Bso31I	GGTCTC	1 cut(s)	1472
Bsp1286I	GDGCHC	4 cut(s)	342, 450, 1612, 1866
Bsp1407I	TGTACA	1 cut(s)	2260
Bsp143I	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
Bsp1720I	GCTNAGC	1 cut(s)	1860
Bsp19I	CCATGG	1 cut(s)	1941
BspACI	CCGC	7 cut(s)	50, 114, 541, 543, 985, 2084, 2087
BspANI	GGCC	3 cut(s)	481, 1305, 1314
BspCNI	CTCAG	2 cut(s)	1543, 1852
BspFNI	CGCG	2 cut(s)	543, 1579
BspHI	TCATGA	3 cut(s)	1540, 1890, 2170
BspLI	GGNNCC	2 cut(s)	461, 1791
BspMAI	CTGCAG	2 cut(s)	617, 2245
BspMI	ACCTGC	2 cut(s)	171, 2234
BspPI	GGATC	1 cut(s)	1722
BspT107I	GGYRCC	1 cut(s)	459
BspTNI	GGTCTC	1 cut(s)	1472
BsrDI	GCAATG	2 cut(s)	319, 627
BsrFI	RCCGGY	3 cut(s)	462, 795, 828
BsrGI	TGTACA	1 cut(s)	2260
BsrI	ACTGG	4 cut(s)	913, 1075, 1279, 1883
BssAI	RCCGGY	3 cut(s)	462, 795, 828
BssECI	CCNNGG	9 cut(s)	306, 307, 433, 541, 576, 865, 1306, 1315, 1941
BssMI	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
BssNI	GRCGYC	3 cut(s)	13, 303, 320
BssT1I	CCWWGG	3 cut(s)	576, 1315, 1941
Bst2UI	CCWGG	7 cut(s)	308, 483, 840, 867, 942, 1307, 1655
Bst4CI	ACNGT	7 cut(s)	260, 584, 1324, 1331, 1357, 1404, 2289
Bst6I	CTCTTC	2 cut(s)	357, 1548
BstACI	GRCGYC	3 cut(s)	13, 303, 320
BstAPI	GCANNNNNTGC	2 cut(s)	80, 1706
BstAUI	TGTACA	1 cut(s)	2260
BstC8I	GCNNGC	1 cut(s)	836
BstDEI	CTNAG	2 cut(s)	1530, 1860
BstDSI	CCRYGG	2 cut(s)	541, 1941
BstF5I	GGATG	3 cut(s)	1351, 1690, 2056
BstFNI	CGCG	2 cut(s)	543, 1579
BstKTI	GATC	6 cut(s)	366, 496, 1041, 1219, 1730, 2128
BstMAI	GTCTC	5 cut(s)	20, 354, 521, 1472, 2180
BstMBI	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
BstMCI	CGRYCG	1 cut(s)	985
BstMWI	GCNNNNNNNGC	7 cut(s)	80, 86, 773, 1106, 1311, 1706, 2027
BstNI	CCWGG	7 cut(s)	308, 483, 840, 867, 942, 1307, 1655
BstNSI	RCATGY	1 cut(s)	771
BstSCI	CCNGG	8 cut(s)	306, 481, 838, 865, 940, 1305, 1653, 2215
BstSFI	CTRYAG	3 cut(s)	613, 1593, 2241
BstUI	CGCG	2 cut(s)	543, 1579
BstX2I	RGATCY	1 cut(s)	1727
BstXI	CCANNNNNNTGG	1 cut(s)	869
BstYI	RGATCY	1 cut(s)	1727
BsuI	GTATCC	1 cut(s)	965
BsuRI	GGCC	3 cut(s)	481, 1305, 1314
BtgI	CCRYGG	2 cut(s)	541, 1941
BtgZI	GCGATG	1 cut(s)	2211
BtsCI	GGATG	3 cut(s)	1351, 1690, 2056
BtsI	GCAGTG	1 cut(s)	520
BtsIMutI	CAGTG	3 cut(s)	520, 1336, 1760
BveI	ACCTGC	2 cut(s)	171, 2234
Cac8I	GCNNGC	1 cut(s)	836
CaiI	CAGNNNCTG	2 cut(s)	77, 80
CciI	TCATGA	3 cut(s)	1540, 1890, 2170
Cfr10I	RCCGGY	3 cut(s)	462, 795, 828
Cfr13I	GGNCC	4 cut(s)	1120, 1789, 1852, 2219
Cfr42I	CCGCGG	1 cut(s)	544
CseI	GACGC	3 cut(s)	311, 568, 1982
Csp6I	GTAC	6 cut(s)	1068, 1320, 1534, 1798, 2238, 2261
CviQI	GTAC	6 cut(s)	1068, 1320, 1534, 1798, 2238, 2261
DdeI	CTNAG	2 cut(s)	1530, 1860
DpnI	GATC	6 cut(s)	365, 495, 1040, 1218, 1729, 2127
DpnII	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
DraI	TTTAAA	2 cut(s)	669, 903
DriI	GACNNNNNGTC	1 cut(s)	1663
EaeI	YGGCCR	2 cut(s)	1303, 1312
Eam1104I	CTCTTC	2 cut(s)	357, 1548
Eam1105I	GACNNNNNGTC	1 cut(s)	1663
EarI	CTCTTC	2 cut(s)	357, 1548
EciI	GGCGGA	1 cut(s)	2102
Ecl136II	GAGCTC	3 cut(s)	448, 1610, 1864
Eco130I	CCWWGG	3 cut(s)	576, 1315, 1941
Eco147I	AGGCCT	1 cut(s)	481
Eco24I	GRGCYC	3 cut(s)	450, 1612, 1866
Eco31I	GGTCTC	1 cut(s)	1472
Eco32I	GATATC	1 cut(s)	883
Eco47I	GGWCC	4 cut(s)	1120, 1789, 1852, 2219
Eco53kI	GAGCTC	3 cut(s)	448, 1610, 1864
EcoICRI	GAGCTC	3 cut(s)	448, 1610, 1864
EcoRI	GAATTC	2 cut(s)	173, 1296
EcoRII	CCWGG	7 cut(s)	306, 481, 838, 865, 940, 1305, 1653
EcoRV	GATATC	1 cut(s)	883
EcoT14I	CCWWGG	3 cut(s)	576, 1315, 1941
EcoT38I	GRGCYC	3 cut(s)	450, 1612, 1866
ErhI	CCWWGG	3 cut(s)	576, 1315, 1941
Esp3I	CGTCTC	1 cut(s)	20
FalI	AAGNNNNNCTT	2 cut(s)	937, 969
FaqI	GGGAC	1 cut(s)	1732
FauI	CCCGC	1 cut(s)	548
FbaI	TGATCA	3 cut(s)	493, 1216, 2125
FblI	GTMKAC	1 cut(s)	1592
FokI	GGATG	3 cut(s)	1358, 1697, 2063
FriOI	GRGCYC	3 cut(s)	450, 1612, 1866
FspBI	CTAG	4 cut(s)	510, 531, 1097, 1919
GsuI	CTGGAG	3 cut(s)	924, 1283, 1626
HaeIII	GGCC	3 cut(s)	481, 1305, 1314
HapII	CCGG	6 cut(s)	463, 695, 796, 829, 1872, 2217
HgaI	GACGC	3 cut(s)	311, 568, 1982
Hin1I	GRCGYC	3 cut(s)	13, 303, 320
HincII	GTYRAC	1 cut(s)	1384
HindII	GTYRAC	1 cut(s)	1384
HinfI	GANTC	6 cut(s)	226, 571, 731, 801, 1423, 1951
HpaI	GTTAAC	1 cut(s)	1384
HpaII	CCGG	6 cut(s)	463, 695, 796, 829, 1872, 2217
HphI	GGTGA	8 cut(s)	198, 272, 334, 503, 927, 1226, 1747, 2205
Hpy166II	GTNNAC	5 cut(s)	1327, 1384, 1593, 1668, 1837
Hpy188I	TCNGA	5 cut(s)	325, 434, 592, 2165, 2257
Hpy188III	TCNNGA	6 cut(s)	1541, 1725, 1867, 1891, 1970, 2171
Hpy8I	GTNNAC	5 cut(s)	1327, 1384, 1593, 1668, 1837
Hpy99I	CGWCG	1 cut(s)	15
HpyAV	CCTTC	5 cut(s)	634, 751, 842, 2049, 2147
HpyCH4III	ACNGT	7 cut(s)	260, 584, 1324, 1331, 1357, 1404, 2289
HpyCH4IV	ACGT	3 cut(s)	13, 320, 2062
HpyF10VI	GCNNNNNNNGC	7 cut(s)	80, 86, 773, 1106, 1311, 1706, 2027
HpyF3I	CTNAG	2 cut(s)	1530, 1860
HpySE526I	ACGT	3 cut(s)	13, 320, 2062
Hsp92I	GRCGYC	3 cut(s)	13, 303, 320
Ksp22I	TGATCA	3 cut(s)	493, 1216, 2125
KspAI	GTTAAC	1 cut(s)	1384
KspI	CCGCGG	1 cut(s)	544
Kzo9I	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
LmnI	GCTCC	5 cut(s)	284, 1195, 1258, 1607, 1945
LweI	GCATC	1 cut(s)	1675
MaeI	CTAG	4 cut(s)	510, 531, 1097, 1919
MaeII	ACGT	3 cut(s)	13, 320, 2062
MaeIII	GTNAC	8 cut(s)	55, 158, 235, 316, 401, 818, 947, 962
MalI	GATC	6 cut(s)	365, 495, 1040, 1218, 1729, 2127
MboI	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
MboII	GAAGA	8 cut(s)	182, 344, 405, 452, 1200, 1565, 2015, 2241
MfeI	CAATTG	1 cut(s)	184
MflI	RGATCY	1 cut(s)	1727
MhlI	GDGCHC	4 cut(s)	342, 450, 1612, 1866
MlsI	TGGCCA	2 cut(s)	1305, 1314
MluNI	TGGCCA	2 cut(s)	1305, 1314
MlyI	GAGTC	2 cut(s)	565, 725
MmeI	TCCRAC	1 cut(s)	2143
Mox20I	TGGCCA	2 cut(s)	1305, 1314
MroXI	GAANNNNTTC	3 cut(s)	1166, 1525, 1557
MscI	TGGCCA	2 cut(s)	1305, 1314
MslI	CAYNNNNRTG	2 cut(s)	867, 1446
Msp20I	TGGCCA	2 cut(s)	1305, 1314
MspA1I	CMGCKG	2 cut(s)	77, 543
MspI	CCGG	6 cut(s)	463, 695, 796, 829, 1872, 2217
MspR9I	CCNGG	8 cut(s)	308, 483, 840, 867, 942, 1307, 1655, 2217
MunI	CAATTG	1 cut(s)	184
MvaI	CCWGG	7 cut(s)	308, 483, 840, 867, 942, 1307, 1655
MvnI	CGCG	2 cut(s)	543, 1579
MwoI	GCNNNNNNNGC	7 cut(s)	80, 86, 773, 1106, 1311, 1706, 2027
NciI	CCSGG	1 cut(s)	2217
NcoI	CCATGG	1 cut(s)	1941
NdeII	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
NlaIV	GGNNCC	2 cut(s)	461, 1791
NmeAIII	GCCGAG	3 cut(s)	26, 1607, 1714
NmuCI	GTSAC	3 cut(s)	316, 401, 962
NspI	RCATGY	1 cut(s)	771
OliI	CACNNNNGTG	1 cut(s)	867
PagI	TCATGA	3 cut(s)	1540, 1890, 2170
PasI	CCCWGGG	1 cut(s)	307
PceI	AGGCCT	1 cut(s)	481
PdmI	GAANNNNTTC	3 cut(s)	1166, 1525, 1557
PfeI	GAWTC	4 cut(s)	226, 801, 1423, 1951
PfoI	TCCNGGA	1 cut(s)	940
PleI	GAGTC	2 cut(s)	565, 725
PpsI	GAGTC	2 cut(s)	565, 725
PshBI	ATTAAT	1 cut(s)	1410
PsiI	TTATAA	1 cut(s)	2280
Psp124BI	GAGCTC	3 cut(s)	450, 1612, 1866
Psp6I	CCWGG	7 cut(s)	306, 481, 838, 865, 940, 1305, 1653
PspGI	CCWGG	7 cut(s)	306, 481, 838, 865, 940, 1305, 1653
PspN4I	GGNNCC	2 cut(s)	461, 1791
PspPI	GGNCC	4 cut(s)	1120, 1789, 1852, 2219
PsrI	GAACNNNNNNTAC	2 cut(s)	2230, 2262
PstI	CTGCAG	2 cut(s)	617, 2245
PstNI	CAGNNNCTG	2 cut(s)	77, 80
PsuI	RGATCY	1 cut(s)	1727
PvuII	CAGCTG	1 cut(s)	77
RsaI	GTAC	6 cut(s)	1069, 1321, 1535, 1799, 2239, 2262
RsaNI	GTAC	6 cut(s)	1068, 1320, 1534, 1798, 2238, 2261
RseI	CAYNNNNRTG	2 cut(s)	867, 1446
SacI	GAGCTC	3 cut(s)	450, 1612, 1866
SacII	CCGCGG	1 cut(s)	544
Sau3AI	GATC	6 cut(s)	363, 493, 1038, 1216, 1727, 2125
Sau96I	GGNCC	4 cut(s)	1120, 1789, 1852, 2219
ScaI	AGTACT	1 cut(s)	1799
SchI	GAGTC	2 cut(s)	565, 725
ScrFI	CCNGG	8 cut(s)	308, 483, 840, 867, 942, 1307, 1655, 2217
SduI	GDGCHC	4 cut(s)	342, 450, 1612, 1866
SfaNI	GCATC	1 cut(s)	1675
SfcI	CTRYAG	3 cut(s)	613, 1593, 2241
SfiI	GGCCNNNNNGGCC	1 cut(s)	1311
Sfr303I	CCGCGG	1 cut(s)	544
SgrBI	CCGCGG	1 cut(s)	544
SinI	GGWCC	4 cut(s)	1120, 1789, 1852, 2219
SmiMI	CAYNNNNRTG	2 cut(s)	867, 1446
SmlI	CTYRAG	3 cut(s)	84, 423, 1977
SmoI	CTYRAG	3 cut(s)	84, 423, 1977
SseBI	AGGCCT	1 cut(s)	481
SsiI	CCGC	7 cut(s)	50, 114, 541, 543, 985, 2084, 2087
SspI	AATATT	3 cut(s)	273, 293, 409
SspMI	CTAG	4 cut(s)	510, 531, 1097, 1919
SstI	GAGCTC	3 cut(s)	450, 1612, 1866
StuI	AGGCCT	1 cut(s)	481
StyD4I	CCNGG	8 cut(s)	306, 481, 838, 865, 940, 1305, 1653, 2215
StyI	CCWWGG	3 cut(s)	576, 1315, 1941
TaaI	ACNGT	7 cut(s)	260, 584, 1324, 1331, 1357, 1404, 2289
TaiI	ACGT	3 cut(s)	16, 323, 2065
TaqI	TCGA	2 cut(s)	2104, 2207
TaqII	GACCGA	1 cut(s)	1496
TatI	WGTACW	3 cut(s)	1533, 1797, 2260
TauI	GCSGC	3 cut(s)	53, 117, 2087
TfiI	GAWTC	4 cut(s)	226, 801, 1423, 1951
TscAI	CASTG	3 cut(s)	520, 1336, 1767
TseFI	GTSAC	3 cut(s)	316, 401, 962
Tsp45I	GTSAC	3 cut(s)	316, 401, 962
TspGWI	ACGGA	3 cut(s)	297, 1401, 1533
TspRI	CASTG	3 cut(s)	520, 1336, 1767
VpaK11BI	GGWCC	4 cut(s)	1120, 1789, 1852, 2219
VspI	ATTAAT	1 cut(s)	1410
XapI	RAATTY	7 cut(s)	173, 649, 931, 1296, 1485, 1505, 2140
XceI	RCATGY	1 cut(s)	771
XmiI	GTMKAC	1 cut(s)	1592
XmnI	GAANNNNTTC	3 cut(s)	1166, 1525, 1557
XspI	CTAG	4 cut(s)	510, 531, 1097, 1919
ZraI	GACGTC	2 cut(s)	14, 321
ZrmI	AGTACT	1 cut(s)	1799

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.