Rorug02G0501600

Protein of unknown function (DUF2854)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005284
Physical Location & Seq
Reverse (-)
63375238 .. 63383354
8117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0501600.1

Sequence Viewer

Length: 2643 bp
ATGAGTAGTGGTGGTGGTGGGTTTAGGGTTTCAATCCCAAACAGTGTGAGGAAGACGATCCAGGACATCAAAGAGATCACGGGCAATCACAGCGAGGAAGAAATCTATGCAATGCTCAAGGAATGTAACATGGATCCCAACGAGACCGCGCAGAAACTTCTGCTTCAGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAGAGAAAAGAGAATCTGAACAACAGAGAGTCTGCAGAGCCACGGTGGAGGCCTGGAATGCCGGGTAGGGGGGGTAGGGGTGGTCGGGTGAATTTTTCATCTCGTCCACCACATGATGCCGGTGGTGGCAGGAACTCAGGTCCTGGAACTGAGAATGGACCCGCTCAGGTTGCAGAGAAAGGTGTTGCTCCCTCTTTGCCAACCACTCGTGAGACAAAAACTAAAGAGAGAAGTCTGGTAACAAGCTCTGTACCTGTGATTGTGGGTGGTCCTTCCAATGTGGCTTCAGGAACTACTACTGTTGTGCCTGCCTCGCAGTTATCTACTGGAACTAGTGCTGAAATAAGTTTGTCATTGGTTGGGGATAAATCGGGTAGCGCGGTCTCCACAGTGGATGCAAAAAAAATCCCCACCAGTGCATTCGGAAATGAGGACTTGCACGAGCAGGCTGCACCAAGCTCCATTAGTAGCTCAGTTTTGCCAAACCCAGCGTCTACATTGGGAGCATGCTTTTCCCCCTCTGATCCTGTACTGGTGCCGTCTAATGATTCACGGCTCCCAGGTTCTGTGGGCACAATCAAACGTGAAGTTGTGAGCCATCATCCTCCTGCTGCTGAGGTTGGCAGCTCTTTAGCACAAGGAAAAACAACAAGCAAAACACAGGGAGTTGGGAAAACTCAGCCCTCTGATCTTTCACATCCTTCATCTACATCAATTCATGGCGGTTCGGTTAGTCGGCCATCATCTAATTATAGTAGCCGGTCGCAACAACTAATTGGCACTCAAAAAGCAGTTGGTACCAACAAGGAGTGGAAACCAAAGCCCATAGTTTCTGCTGTTGTTCAAGGTCAAGCAACAGCTAATGTAGCTGCACCTGAGGTGCCTGCTGATTTGGTAGAGGTCTCTCCGCAGTCACAACCTGTTTCAAGTGTCCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAAGAAGCTAGAGGAATTACACCTTCCACAGCTTCCACAGCGCAAATTAGTTATTCTTCCAAATCACATACACGTTCCTGAGTCTGAACGAAACAAGTTGAGTTTTGGAAGCTTTGGGGCAACTTTTGGCATAACGAATAGCTATGTCAGTGGTCCTGAGAGTGAGAAGAGCTCTACTCCTCGGTCTGAAACTTCTCAGGTTATTGAAGAATCTGTGGAGGAACAGTCCTCAAGCAATCAAAATGTGTTGGCAACTGCCGATGAGGGTGATTTTCCTGATCATTCGCAATCACCTACTCATATGCTTGAAAATCTATCATCTGGTGAGGGCGATGTCTCATCCAGTGCATCCCAAGGACATAATGAATCTAAGCATGACACTGTATTGACTTCTGGAGGTCATCAGCTCCCAGTGGCTAATACTTCTCCGAATTATAGTTTTGGTATTGTACCTCCAATTTTAGGGAGTCAGCTTGCGGCATTTGAAAACTCTGAATCTCAAGCACGTGATATATCTCGGCTTCCAAACTTTGTAGTACAACAACCCCTTGACCCAGCAAGTTATTATGCCCAATTTTACCGCTCAGGTGCTGATAGTGATGGCCGTCTTTCTCCTTTTCCATCTCCTGGGGTATCATCAAAGTACAATGGCAATGTTGGAGTATTGCCTCCAAGTTCTCAGTCTCCACAAGAGGGTGGGGTTCTTTCAGCAGCAGGTCCAACACCATTGGTGACACAGGCTTCTGGGCTCGTGCAAAGCTCAATAGCTGTAACTCAGCAGCCAGTTCCTGTCTTCCGACCAACAGCTGGGGTGCACATACCTCATTATGCTAACTACATTCCTTACAGTCACTACTTTAACCCATTCTATGTTCCACCTCCTATCCACCAATATTTGGGTAATGGAGCATTTCCTCAACAACCTCAAGCAGGTGGTGTGTATCCAGCTCCACCAGCAGCTGCTGCCACAGGAGTCAAATACTCACTGCCACAATACAAAGCAGGGACTAATACAGGAAACTCAAATCACATGGGAATGGCAAGTGGATATGGGCCCTATGGCTCTTCCCCAGCTGGTTATAATCCCAGTCCTGCCACGACTGCAGGAAACACTACTCCCAATGAAGATCTTAGCACATCTCAGTTCAAAGAGAGTAATGTATACATCACCGGACAGCAGAGTGAAGGTTCATCTGTATGGGTTGCTGCACCTAACCGAGAAATGCCCAGCTTAACAAGTTCATTTTACAACCTTCCTCCACAGGGTCAGCATGTGACTTTCACTCCAACGCAGGCCGGCCATGGTACCTTTGCTGGTCTCTACCACCCTGCACAAGCAGTATCAGCTGCAGCTGTCCATCCACTTCTGCAACAGTCCCAAACCATGGCTGGGGCAGTTGACATGGTGGGACCTGGGGGAAGTGTTTATCAGCAGCCTCAGCATGCACAACAAATCAACTGGCCGAGCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2854 UMP1

Protein Analysis

880

Amino Acids

92.32

Weight (kDa)

6.3

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 12 - 71 1e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2247
AarI CACCTGC 1 cut(s) 2087
Acc36I ACCTGC 2 cut(s) 1871, 2087
Acc65I GGTACC 2 cut(s) 998, 2471
AccB1I GGYRCC 4 cut(s) 736, 998, 1081, 2471
AccB7I CCANNNNNTGG 4 cut(s) 1793, 1973, 2062, 2551
AccBSI CCGCTC 2 cut(s) 365, 1749
AccI GTMKAC 2 cut(s) 695, 2328
AccII CGCG 2 cut(s) 149, 581
AciI CCGC 7 cut(s) 147, 363, 581, 924, 1109, 1643, 1747
AclWI GGATC 6 cut(s) 52, 128, 141, 164, 177, 719
AcoI YGGCCR 4 cut(s) 938, 1768, 2464, 2627
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 2 cut(s) 149, 471
AcvI CACGTG 1 cut(s) 1673
AfaI GTAC 7 cut(s) 453, 732, 1000, 1617, 1704, 1811, 2473
AflIII ACRYGT 1 cut(s) 1237
AgsI TTSAA 8 cut(s) 33, 1046, 1128, 1158, 1373, 1475, 1652, 2314
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 6 cut(s) 60, 253, 343, 760, 1792, 2578
Alw21I GWGCWC 2 cut(s) 1340, 1983
Alw26I GTCTC 7 cut(s) 137, 407, 589, 1108, 1507, 1854, 2489
Alw44I GTGCAC 1 cut(s) 1979
AlwI GGATC 6 cut(s) 52, 128, 141, 164, 177, 719
AlwNI CAGNNNCTG 6 cut(s) 344, 767, 1757, 1955, 2126, 2129
AoxI GGCC 7 cut(s) 251, 938, 1768, 2219, 2460, 2464, 2627
ApaI GGGCCC 1 cut(s) 2223
ApaLI GTGCAC 1 cut(s) 1979
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp718I GGTACC 2 cut(s) 998, 2471
AspLEI GCGC 3 cut(s) 151, 581, 1209
AspS9I GGNCC 8 cut(s) 341, 359, 470, 1319, 1883, 2219, 2220, 2576
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 6 cut(s) 301, 1445, 1449, 1502, 1909, 2326
AvaII GGWCC 6 cut(s) 341, 359, 470, 1319, 1883, 2576
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 3 cut(s) 776, 1983, 2223
BamHI GGATCC 2 cut(s) 133, 169
BanI GGYRCC 4 cut(s) 736, 998, 1081, 2471
BanII GRGCYC 3 cut(s) 1340, 1917, 2223
BauI CACGAG 3 cut(s) 408, 641, 1916
BbrPI CACGTG 1 cut(s) 1673
BbsI GAAGAC 2 cut(s) 59, 1951
Bbv12I GWGCWC 2 cut(s) 1340, 1983
BbvCI CCTCAGC 2 cut(s) 816, 2604
BccI CCATC 5 cut(s) 807, 949, 1760, 1795, 2532
BceAI ACGGC 3 cut(s) 724, 770, 1755
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciT130I CCWGG 6 cut(s) 62, 255, 345, 762, 1794, 2580
BciVI GTATCC 1 cut(s) 2118
BclI TGATCA 1 cut(s) 1444
BcnI CCSGG 1 cut(s) 264
BcoDI GTCTC 7 cut(s) 137, 407, 589, 1108, 1507, 1854, 2489
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 2 cut(s) 534, 1175
BfmI CTRYAG 4 cut(s) 234, 1163, 2268, 2514
BfuAI ACCTGC 2 cut(s) 1871, 2087
BfuI GTATCC 1 cut(s) 2118
BglII AGATCT 1 cut(s) 2293
Bme1390I CCNGG 7 cut(s) 62, 255, 264, 345, 762, 1794, 2580
Bme18I GGWCC 6 cut(s) 341, 359, 470, 1319, 1883, 2576
BmgT120I GGNCC 8 cut(s) 341, 359, 470, 1319, 1883, 2219, 2220, 2576
BmrFI CCNGG 7 cut(s) 62, 255, 264, 345, 762, 1794, 2580
BmrI ACTGGG 2 cut(s) 1571, 2247
BmsI GCATC 3 cut(s) 307, 586, 1523
BmuI ACTGGG 2 cut(s) 1571, 2247
BpiI GAAGAC 2 cut(s) 59, 1951
BplI GAGNNNNNCTC 4 cut(s) 1322, 1354, 1327, 1359
BpmI CTGGAG 1 cut(s) 1581
Bpu10I CCTNAGC 4 cut(s) 366, 816, 1750, 2604
BpuEI CTTGAG 4 cut(s) 101, 1381, 1650, 2076
BpuMI CCSGG 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 1673
BsaI GGTCTC 4 cut(s) 137, 589, 1108, 2489
BsaJI CCNNGG 8 cut(s) 242, 760, 1346, 1519, 1793, 2467, 2550, 2579
BsaWI WCCGGW 1 cut(s) 2336
BsaXI ACNNNNNCTCC 4 cut(s) 1376, 1406, 2434, 2464
Bse118I RCCGGY 3 cut(s) 320, 960, 2462
Bse1I ACTGG 8 cut(s) 532, 615, 738, 1509, 1577, 1949, 2253, 2630
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 2 cut(s) 117, 1825
BseBI CCWGG 6 cut(s) 62, 255, 345, 762, 1794, 2580
BseDI CCNNGG 8 cut(s) 242, 760, 1346, 1519, 1793, 2467, 2550, 2579
BseGI GGATG 6 cut(s) 601, 802, 898, 1505, 1514, 2524
BseMI GCAATG 2 cut(s) 117, 1825
BseNI ACTGG 8 cut(s) 532, 615, 738, 1509, 1577, 1949, 2253, 2630
BseRI GAGGAG 1 cut(s) 1335
BseSI GKGCMC 3 cut(s) 776, 1983, 2223
BseYI CCCAGC 6 cut(s) 688, 1720, 1973, 2236, 2393, 2555
BsgI GTGCAG 4 cut(s) 636, 1056, 2358, 2481
Bsh1236I CGCG 2 cut(s) 149, 581
Bsh1285I CGRYCG 1 cut(s) 965
BshFI GGCC 7 cut(s) 253, 940, 1770, 2221, 2462, 2466, 2629
BshNI GGYRCC 4 cut(s) 736, 998, 1081, 2471
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 2 cut(s) 1340, 1983
BsiSI CCGG 5 cut(s) 263, 321, 961, 2337, 2463
BslFI GGGAC 3 cut(s) 2185, 2527, 2589
BsmAI GTCTC 7 cut(s) 137, 407, 589, 1108, 1507, 1854, 2489
BsmFI GGGAC 3 cut(s) 2185, 2527, 2589
BsmI GAATGC 2 cut(s) 264, 620
BsnI GGCC 7 cut(s) 253, 940, 1770, 2221, 2462, 2466, 2629
Bso31I GGTCTC 4 cut(s) 137, 589, 1108, 2489
Bsp120I GGGCCC 1 cut(s) 2219
Bsp1286I GDGCHC 5 cut(s) 776, 1340, 1917, 1983, 2223
Bsp143I GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
Bsp19I CCATGG 2 cut(s) 2467, 2550
BspACI CCGC 7 cut(s) 147, 363, 581, 924, 1109, 1643, 1747
BspANI GGCC 7 cut(s) 253, 940, 1770, 2221, 2462, 2466, 2629
BspFNI CGCG 2 cut(s) 149, 581
BspHI TCATGA 1 cut(s) 178
BspMAI CTGCAG 4 cut(s) 238, 1167, 2272, 2518
BspMI ACCTGC 2 cut(s) 1871, 2087
BspPI GGATC 6 cut(s) 52, 128, 141, 164, 177, 719
BspQI GCTCTTC 2 cut(s) 1328, 2236
BspT107I GGYRCC 4 cut(s) 736, 998, 1081, 2471
BspTNI GGTCTC 4 cut(s) 137, 589, 1108, 2489
BsrBI CCGCTC 2 cut(s) 365, 1749
BsrDI GCAATG 2 cut(s) 117, 1825
BsrFI RCCGGY 3 cut(s) 320, 960, 2462
BsrI ACTGG 8 cut(s) 532, 615, 738, 1509, 1577, 1949, 2253, 2630
BssAI RCCGGY 3 cut(s) 320, 960, 2462
BssECI CCNNGG 8 cut(s) 242, 760, 1346, 1519, 1793, 2467, 2550, 2579
BssMI GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
BssNAI GTATAC 1 cut(s) 2329
BssSI CACGAG 3 cut(s) 408, 641, 1916
BssT1I CCWWGG 3 cut(s) 1519, 2467, 2550
Bst1107I GTATAC 1 cut(s) 2329
Bst2BI CACGAG 3 cut(s) 408, 641, 1916
Bst2UI CCWGG 6 cut(s) 62, 255, 345, 762, 1794, 2580
Bst4CI ACNGT 8 cut(s) 44, 246, 502, 592, 1392, 1549, 2015, 2541
Bst6I CTCTTC 2 cut(s) 1328, 2236
BstAPI GCANNNNNTGC 1 cut(s) 2129
BstBAI YACGTR 1 cut(s) 1673
BstC8I GCNNGC 8 cut(s) 510, 648, 709, 1086, 1641, 2460, 2464, 2610
BstDSI CCRYGG 3 cut(s) 242, 2467, 2550
BstENI CCTNNNNNAGG 1 cut(s) 2094
BstF5I GGATG 6 cut(s) 601, 802, 898, 1505, 1514, 2524
BstFNI CGCG 2 cut(s) 149, 581
BstHHI GCGC 3 cut(s) 151, 581, 1209
BstKTI GATC 8 cut(s) 60, 78, 136, 172, 727, 892, 1447, 2296
BstMAI GTCTC 7 cut(s) 137, 407, 589, 1108, 1507, 1854, 2489
BstMBI GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
BstMCI CGRYCG 1 cut(s) 965
BstNI CCWGG 6 cut(s) 62, 255, 345, 762, 1794, 2580
BstNSI RCATGY 3 cut(s) 711, 2441, 2612
BstSCI CCNGG 7 cut(s) 60, 253, 262, 343, 760, 1792, 2578
BstSFI CTRYAG 4 cut(s) 234, 1163, 2268, 2514
BstSLI GKGCMC 3 cut(s) 776, 1983, 2223
BstUI CGCG 2 cut(s) 149, 581
BstV2I GAAGAC 2 cut(s) 59, 1951
BstX2I RGATCY 3 cut(s) 133, 169, 2293
BstYI RGATCY 3 cut(s) 133, 169, 2293
BstZ17I GTATAC 1 cut(s) 2329
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 1 cut(s) 2118
BsuRI GGCC 7 cut(s) 253, 940, 1770, 2221, 2462, 2466, 2629
BtgI CCRYGG 3 cut(s) 242, 2467, 2550
BtgZI GCGATG 1 cut(s) 1512
BtsCI GGATG 6 cut(s) 601, 802, 898, 1505, 1514, 2524
BtsI GCAGTG 1 cut(s) 2150
BtsIMutI CAGTG 8 cut(s) 49, 597, 622, 1321, 1516, 1545, 1584, 2150
BveI ACCTGC 2 cut(s) 1871, 2087
Cac8I GCNNGC 8 cut(s) 510, 648, 709, 1086, 1641, 2460, 2464, 2610
CaiI CAGNNNCTG 6 cut(s) 344, 767, 1757, 1955, 2126, 2129
CciI TCATGA 1 cut(s) 178
CfoI GCGC 3 cut(s) 151, 581, 1209
Cfr10I RCCGGY 3 cut(s) 320, 960, 2462
Cfr13I GGNCC 8 cut(s) 341, 359, 470, 1319, 1883, 2219, 2220, 2576
CseI GACGC 1 cut(s) 681
Csp6I GTAC 7 cut(s) 452, 731, 999, 1616, 1703, 1810, 2472
CviQI GTAC 7 cut(s) 452, 731, 999, 1616, 1703, 1810, 2472
DpnI GATC 8 cut(s) 59, 77, 135, 171, 726, 891, 1446, 2295
DpnII GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
EaeI YGGCCR 4 cut(s) 938, 1768, 2464, 2627
Eam1104I CTCTTC 2 cut(s) 1328, 2236
EarI CTCTTC 2 cut(s) 1328, 2236
Ecl136II GAGCTC 1 cut(s) 1338
Eco130I CCWWGG 3 cut(s) 1519, 2467, 2550
Eco147I AGGCCT 1 cut(s) 253
Eco24I GRGCYC 3 cut(s) 1340, 1917, 2223
Eco31I GGTCTC 4 cut(s) 137, 589, 1108, 2489
Eco47I GGWCC 6 cut(s) 341, 359, 470, 1319, 1883, 2576
Eco53kI GAGCTC 1 cut(s) 1338
Eco57I CTGAAG 2 cut(s) 149, 471
Eco72I CACGTG 1 cut(s) 1673
Eco81I CCTNAGG 1 cut(s) 1077
EcoICRI GAGCTC 1 cut(s) 1338
EcoNI CCTNNNNNAGG 1 cut(s) 2094
EcoO109I RGGNCCY 3 cut(s) 341, 2220, 2576
EcoRII CCWGG 6 cut(s) 60, 253, 343, 760, 1792, 2578
EcoT14I CCWWGG 3 cut(s) 1519, 2467, 2550
EcoT38I GRGCYC 3 cut(s) 1340, 1917, 2223
ErhI CCWWGG 3 cut(s) 1519, 2467, 2550
FaqI GGGAC 3 cut(s) 2185, 2527, 2589
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 1467
FbaI TGATCA 1 cut(s) 1444
FblI GTMKAC 2 cut(s) 695, 2328
FokI GGATG 6 cut(s) 608, 789, 885, 1492, 1501, 2511
FriOI GRGCYC 3 cut(s) 1340, 1917, 2223
FseI GGCCGGCC 1 cut(s) 2466
FspBI CTAG 2 cut(s) 534, 1175
GlaI GCGC 3 cut(s) 150, 580, 1208
GsaI CCCAGC 6 cut(s) 692, 1724, 1977, 2240, 2397, 2559
GsuI CTGGAG 1 cut(s) 1581
HaeIII GGCC 7 cut(s) 253, 940, 1770, 2221, 2462, 2466, 2629
HapII CCGG 5 cut(s) 263, 321, 961, 2337, 2463
HgaI GACGC 1 cut(s) 681
HhaI GCGC 3 cut(s) 151, 581, 1209
Hin6I GCGC 3 cut(s) 149, 579, 1207
HinP1I GCGC 3 cut(s) 149, 579, 1207
HincII GTYRAC 1 cut(s) 2566
HindII GTYRAC 1 cut(s) 2566
HindIII AAGCTT 1 cut(s) 1276
HpaII CCGG 5 cut(s) 263, 321, 961, 2337, 2463
HphI GGTGA 6 cut(s) 301, 1445, 1449, 1502, 1909, 2326
Hpy166II GTNNAC 5 cut(s) 308, 696, 1981, 2329, 2566
Hpy188III TCNNGA 8 cut(s) 167, 179, 410, 489, 1244, 1322, 1442, 1560
Hpy8I GTNNAC 5 cut(s) 308, 696, 1981, 2329, 2566
HpyAV CCTTC 5 cut(s) 483, 912, 1199, 2345, 2429
HpyCH4III ACNGT 8 cut(s) 44, 246, 502, 592, 1392, 1549, 2015, 2541
HpyCH4IV ACGT 4 cut(s) 196, 784, 1239, 1672
HpySE526I ACGT 4 cut(s) 196, 784, 1239, 1672
HspAI GCGC 3 cut(s) 149, 579, 1207
KpnI GGTACC 2 cut(s) 1002, 2475
KroI GCCGGC 1 cut(s) 2462
KroNI GCCGGC 1 cut(s) 2464
Ksp22I TGATCA 1 cut(s) 1444
Kzo9I GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
LguI GCTCTTC 2 cut(s) 1328, 2236
LmnI GCTCC 7 cut(s) 394, 665, 704, 762, 1578, 2072, 2119
LweI GCATC 3 cut(s) 307, 586, 1523
MaeI CTAG 2 cut(s) 534, 1175
MaeII ACGT 4 cut(s) 196, 784, 1239, 1672
MaeIII GTNAC 8 cut(s) 125, 197, 439, 1113, 1897, 1936, 2015, 2440
MalI GATC 8 cut(s) 59, 77, 135, 171, 726, 891, 1446, 2295
MbiI CCGCTC 2 cut(s) 365, 1749
MboI GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
MflI RGATCY 3 cut(s) 133, 169, 2293
MhlI GDGCHC 5 cut(s) 776, 1340, 1917, 1983, 2223
MluCI AATT 9 cut(s) 292, 915, 949, 975, 1181, 1211, 1597, 1623, 1739
MlyI GAGTC 4 cut(s) 239, 1256, 1642, 2148
MmeI TCCRAC 4 cut(s) 1804, 1910, 1987, 2477
MroNI GCCGGC 1 cut(s) 2462
MseI TTAA 2 cut(s) 2025, 2399
MslI CAYNNNNRTG 2 cut(s) 2201, 2362
MspA1I CMGCKG 5 cut(s) 1973, 2126, 2240, 2513, 2519
MspI CCGG 5 cut(s) 263, 321, 961, 2337, 2463
MspR9I CCNGG 7 cut(s) 62, 255, 264, 345, 762, 1794, 2580
Mva1269I GAATGC 2 cut(s) 264, 620
MvaI CCWGG 6 cut(s) 62, 255, 345, 762, 1794, 2580
MvnI CGCG 2 cut(s) 149, 581
NaeI GCCGGC 1 cut(s) 2464
NciI CCSGG 1 cut(s) 264
NcoI CCATGG 2 cut(s) 2467, 2550
NdeI CATATG 1 cut(s) 1467
NdeII GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
NgoMIV GCCGGC 1 cut(s) 2462
NmeAIII GCCGAG 1 cut(s) 1663
NmuCI GTSAC 5 cut(s) 197, 1113, 1897, 2015, 2440
NspI RCATGY 3 cut(s) 711, 2441, 2612
PaeI GCATGC 2 cut(s) 711, 2612
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 2087
PceI AGGCCT 1 cut(s) 253
PciSI GCTCTTC 2 cut(s) 1328, 2236
PctI GAATGC 2 cut(s) 264, 620
PdiI GCCGGC 1 cut(s) 2464
PfeI GAWTC 6 cut(s) 214, 749, 1139, 1376, 1532, 1661
PflMI CCANNNNNTGG 4 cut(s) 1793, 1973, 2062, 2551
PfoI TCCNGGA 2 cut(s) 60, 343
PleI GAGTC 4 cut(s) 238, 1255, 1641, 2147
PmaCI CACGTG 1 cut(s) 1673
PmlI CACGTG 1 cut(s) 1673
PpsI GAGTC 4 cut(s) 238, 1255, 1641, 2147
Ppu21I YACGTR 1 cut(s) 1673
PpuMI RGGWCCY 2 cut(s) 341, 2576
PsiI TTATAA 1 cut(s) 2247
Psp124BI GAGCTC 1 cut(s) 1340
Psp5II RGGWCCY 2 cut(s) 341, 2576
Psp6I CCWGG 6 cut(s) 60, 253, 343, 760, 1792, 2578
PspCI CACGTG 1 cut(s) 1673
PspFI CCCAGC 6 cut(s) 688, 1720, 1973, 2236, 2393, 2555
PspGI CCWGG 6 cut(s) 60, 253, 343, 760, 1792, 2578
PspOMI GGGCCC 1 cut(s) 2219
PspPI GGNCC 8 cut(s) 341, 359, 470, 1319, 1883, 2219, 2220, 2576
PspPPI RGGWCCY 2 cut(s) 341, 2576
PstI CTGCAG 4 cut(s) 238, 1167, 2272, 2518
PstNI CAGNNNCTG 6 cut(s) 344, 767, 1757, 1955, 2126, 2129
PsuI RGATCY 3 cut(s) 133, 169, 2293
PvuII CAGCTG 5 cut(s) 1973, 2126, 2240, 2513, 2519
RigI GGCCGGCC 1 cut(s) 2466
RsaI GTAC 7 cut(s) 453, 732, 1000, 1617, 1704, 1811, 2473
RsaNI GTAC 7 cut(s) 452, 731, 999, 1616, 1703, 1810, 2472
RseI CAYNNNNRTG 2 cut(s) 2201, 2362
SacI GAGCTC 1 cut(s) 1340
SapI GCTCTTC 2 cut(s) 1328, 2236
SaqAI TTAA 2 cut(s) 2025, 2399
Sau3AI GATC 8 cut(s) 57, 75, 133, 169, 724, 889, 1444, 2293
Sau96I GGNCC 8 cut(s) 341, 359, 470, 1319, 1883, 2219, 2220, 2576
SchI GAGTC 4 cut(s) 239, 1256, 1642, 2148
ScrFI CCNGG 7 cut(s) 62, 255, 264, 345, 762, 1794, 2580
SduI GDGCHC 5 cut(s) 776, 1340, 1917, 1983, 2223
SfaNI GCATC 3 cut(s) 307, 586, 1523
SfcI CTRYAG 4 cut(s) 234, 1163, 2268, 2514
SinI GGWCC 6 cut(s) 341, 359, 470, 1319, 1883, 2576
SmiMI CAYNNNNRTG 2 cut(s) 2201, 2362
SmlI CTYRAG 4 cut(s) 116, 1396, 1665, 2091
SmoI CTYRAG 4 cut(s) 116, 1396, 1665, 2091
SpeI ACTAGT 1 cut(s) 533
SphI GCATGC 2 cut(s) 711, 2612
Sse9I AATT 9 cut(s) 292, 915, 949, 975, 1181, 1211, 1597, 1623, 1739
SseBI AGGCCT 1 cut(s) 253
SsiI CCGC 7 cut(s) 147, 363, 581, 924, 1109, 1643, 1747
SspI AATATT 1 cut(s) 2060
SspMI CTAG 2 cut(s) 534, 1175
SstI GAGCTC 1 cut(s) 1340
StuI AGGCCT 1 cut(s) 253
StyD4I CCNGG 7 cut(s) 60, 253, 262, 343, 760, 1792, 2578
StyI CCWWGG 3 cut(s) 1519, 2467, 2550
TaaI ACNGT 8 cut(s) 44, 246, 502, 592, 1392, 1549, 2015, 2541
TaiI ACGT 4 cut(s) 199, 787, 1242, 1675
TaqII GACCGA 1 cut(s) 1338
TasI AATT 9 cut(s) 292, 915, 949, 975, 1181, 1211, 1597, 1623, 1739
TatI WGTACW 3 cut(s) 730, 1702, 1809
TauI GCSGC 1 cut(s) 1646
TfiI GAWTC 6 cut(s) 214, 749, 1139, 1376, 1532, 1661
Tru1I TTAA 2 cut(s) 2025, 2399
Tru9I TTAA 2 cut(s) 2025, 2399
TscAI CASTG 8 cut(s) 49, 597, 622, 1321, 1516, 1552, 1584, 2157
TseFI GTSAC 5 cut(s) 197, 1113, 1897, 2015, 2440
Tsp45I GTSAC 5 cut(s) 197, 1113, 1897, 2015, 2440
TspDTI ATGAA 8 cut(s) 167, 288, 894, 908, 1545, 2304, 2346, 2397
TspRI CASTG 8 cut(s) 49, 597, 622, 1321, 1516, 1552, 1584, 2157
Van91I CCANNNNNTGG 4 cut(s) 1793, 1973, 2062, 2551
VneI GTGCAC 1 cut(s) 1979
VpaK11BI GGWCC 6 cut(s) 341, 359, 470, 1319, 1883, 2576
XagI CCTNNNNNAGG 1 cut(s) 2094
XapI RAATTY 1 cut(s) 292
XceI RCATGY 3 cut(s) 711, 2441, 2612
XcmI CCANNNNNNNNNTGG 1 cut(s) 2552
XmiI GTMKAC 2 cut(s) 695, 2328
XspI CTAG 2 cut(s) 534, 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.