Rorug04G0124600

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002325
Physical Location & Seq
Forward (+)
19725362 .. 19731358
5997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0124600.1

Sequence Viewer

Length: 549 bp
ATGGGCATTTTGTTCACGAAAATGTTCTCTTCCCTTTTTGGGAACAAGGAGGCTCGGATCCTCGTCCTCGGATTGGACAATGCTGGCAAAACCACTATTCTCTATCGGCTGCAGATGGGGGAGGTTGTGTCCACAATTCCAACGATTGGATTCAATGTTGAGACAGTGCAGTACAACAACATCAAATTCCAAGTATGGGATCTCGGTGGACAGACAAGTATCAGGCCATATTGGAGATGCTATTTTCCAAATACTCAAGCCATAATCTATGTTGTTGACTCTAGCGACACTGACAGGCTGGTAATAGCTAAAGAGGAGTTTCATGCTATCTTGGAGGAGGAAGAGTTGAAGGGTGCAGTTGTCCTTATTTTTGCCAATAAGCAGGATCTTCCTGGTGCACTTGATGATGCTGCAATAACTGAGGCCTTAGAGTTGCACAAGATAAAAAACCGTCAATGGTCTATCTTTAAAACTTCTGCCATAAAAGGCGAAGGTCTTTTTGAGGGCTTAGACTGGCTGAGTAACACACTGAAGTCTGGAGGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000184 GO:0000278 GO:0000956 GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006417 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010212 GO:0010332 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0015031 GO:0015833 GO:0015934 GO:0016070 GO:0016071 GO:0016072 GO:0019222 GO:0019439 GO:0019538 GO:0022402 GO:0022613 GO:0022625 GO:0022626 GO:0023051 GO:0023052 GO:0023056 GO:0030330 GO:0030424 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031570 GO:0031571 GO:0031667 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033036 GO:0033267 GO:0033365 GO:0033554 GO:0034248 GO:0034250 GO:0034470 GO:0034613 GO:0034641 GO:0034644 GO:0034645 GO:0034655 GO:0034660 GO:0035556 GO:0042254 GO:0042273 GO:0042594 GO:0042770 GO:0042886 GO:0042981 GO:0042995 GO:0043005 GO:0043043 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043195 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043516 GO:0043517 GO:0043603 GO:0043604 GO:0043679 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044306 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045047 GO:0045184 GO:0045202 GO:0045727 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0046907 GO:0048027 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051641 GO:0051649 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070972 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071482 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0072594 GO:0072599 GO:0072657 GO:0080090 GO:0080134 GO:0080135 GO:0090068 GO:0090150 GO:0090304 GO:0097159 GO:0097458 GO:0098793 GO:0104004 GO:0120025 GO:0120038 GO:0150034 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901796 GO:1901798 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902162 GO:1902164 GO:1902165 GO:1902167 GO:1902229 GO:1902231 GO:1902253 GO:1902255 GO:1902400 GO:1902402 GO:1902403 GO:1902531 GO:1902533 GO:1902806 GO:1902807 GO:1903047 GO:1904803 GO:1990904 GO:1990928 GO:2000045 GO:2000112 GO:2000134 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KOW Ribosomal_L26

Protein Analysis

182

Amino Acids

20.34

Weight (kDa)

5.25

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 5 - 176 1.4e-79 ADP-ribosylation factor family
SRPRB PF09439 16 - 151 9.6e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 1e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 170 4.5e-11 Ras family
Gtr1_RagA PF04670 19 - 130 5.8e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 44 - 130 3.7e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 146
AclWI GGATC 4 cut(s) 52, 65, 207, 393
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 39, 73, 146, 196
AgsI TTSAA 2 cut(s) 154, 349
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 1 cut(s) 308
AluI AGCT 1 cut(s) 308
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 155
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 4 cut(s) 52, 65, 207, 393
AoxI GGCC 2 cut(s) 224, 423
ApaLI GTGCAC 1 cut(s) 396
ApeKI GCWGC 2 cut(s) 109, 410
ApoI RAATTY 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 400
BamHI GGATCC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 96, 397
BccI CCATC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 110
BisI GCNGC 2 cut(s) 110, 411
BlsI GCNGC 2 cut(s) 111, 412
Bme1390I CCNGG 1 cut(s) 393
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 2 cut(s) 227, 397
BpuEI CTTGAG 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 67
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 329, 359
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 73, 146, 196
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 4 cut(s) 39, 73, 146, 196
BseMII CTCAG 2 cut(s) 411, 509
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 2 cut(s) 329, 350
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 2 cut(s) 96, 397
BsgI GTGCAG 2 cut(s) 188, 375
BshFI GGCC 2 cut(s) 226, 425
BsiHKAI GWGCWC 1 cut(s) 400
BslI CCNNNNNNNGG 4 cut(s) 39, 73, 146, 196
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 226, 425
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 57, 199, 385
BspANI GGCC 2 cut(s) 226, 425
BspCNI CTCAG 2 cut(s) 412, 510
BspLI GGNNCC 1 cut(s) 59
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 4 cut(s) 52, 65, 207, 393
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 3 cut(s) 57, 199, 385
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 166, 452
Bst6I CTCTTC 2 cut(s) 34, 336
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 4 cut(s) 420, 427, 508, 518
BstKTI GATC 3 cut(s) 60, 202, 388
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 57, 199, 385
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 2 cut(s) 96, 397
BstX2I RGATCY 3 cut(s) 57, 199, 385
BstYI RGATCY 3 cut(s) 57, 199, 385
BsuRI GGCC 2 cut(s) 226, 425
BtsIMutI CAGTG 3 cut(s) 171, 288, 527
Cac8I GCNNGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 172
CviAII CATG 1 cut(s) 323
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 4 cut(s) 420, 427, 508, 518
DpnI GATC 3 cut(s) 59, 201, 387
DpnII GATC 3 cut(s) 57, 199, 385
DraI TTTAAA 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 34, 336
EarI CTCTTC 2 cut(s) 34, 336
Eco147I AGGCCT 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 326
FaiI YATR 6 cut(s) 196, 229, 263, 270, 324, 482
FatI CATG 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 110, 411
Fsp4HI GCNGC 2 cut(s) 110, 411
FspBI CTAG 1 cut(s) 282
GluI GCNGC 2 cut(s) 110, 411
HaeIII GGCC 2 cut(s) 226, 425
Hin1II CATG 1 cut(s) 326
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 150, 278
Hpy166II GTNNAC 5 cut(s) 15, 132, 209, 277, 398
Hpy188I TCNGA 2 cut(s) 57, 71
Hpy188III TCNNGA 2 cut(s) 16, 537
Hpy8I GTNNAC 5 cut(s) 15, 132, 209, 277, 398
HpyAV CCTTC 2 cut(s) 343, 485
HpyCH4III ACNGT 2 cut(s) 166, 452
HpyCH4V TGCA 6 cut(s) 112, 169, 356, 398, 413, 436
HpyF3I CTNAG 4 cut(s) 420, 427, 508, 518
Hsp92II CATG 1 cut(s) 326
Kzo9I GATC 3 cut(s) 57, 199, 385
LpnPI CCDG 9 cut(s) 69, 208, 280, 284, 368, 378, 405, 499, 522
Lsp1109I GCAGC 2 cut(s) 96, 397
LweI GCATC 2 cut(s) 227, 397
MaeI CTAG 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 521
MalI GATC 3 cut(s) 59, 201, 387
MboI GATC 3 cut(s) 57, 199, 385
MboII GAAGA 3 cut(s) 21, 353, 380
MflI RGATCY 3 cut(s) 57, 199, 385
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 2 cut(s) 135, 185
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
NdeII GATC 3 cut(s) 57, 199, 385
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 1 cut(s) 59
PceI AGGCCT 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 2 cut(s) 111, 412
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 59
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 3 cut(s) 57, 199, 385
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 2 cut(s) 110, 411
Sau3AI GATC 3 cut(s) 57, 199, 385
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 400
SetI ASST 4 cut(s) 126, 310, 496, 544
SfaNI GCATC 2 cut(s) 227, 397
SfcI CTRYAG 1 cut(s) 110
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 2 cut(s) 135, 185
SseBI AGGCCT 1 cut(s) 425
SspMI CTAG 1 cut(s) 282
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 166, 452
TasI AATT 2 cut(s) 135, 185
TatI WGTACW 1 cut(s) 171
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 3 cut(s) 171, 295, 534
TseI GCWGC 2 cut(s) 109, 410
TspDTI ATGAA 1 cut(s) 311
TspRI CASTG 3 cut(s) 171, 295, 534
Van91I CCANNNNNTGG 1 cut(s) 146
VneI GTGCAC 1 cut(s) 396
XapI RAATTY 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.