Rorug06G0024200

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003667
Physical Location & Seq
Reverse (-)
2973123 .. 2975261
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0024200.1

Sequence Viewer

Length: 1449 bp
ATGGGAGACAAGAAACCAAAGCATCTTTCTGGTTGCTCAAAGCTTGACAAACTGCCGGATGAGTTGGGTCATGTTGCCTGTTTGGAGAATCTTGATGTGAGTGGAAGTGGCATAAGAGAACTGCCCTCCTCTATTGGCATGCTTGAAGGACTTGTTTCTATATCGTTGAGAGATTGCAAACATCTTCACTGTCTTCCAAGCAGTGTAGGTGGCTTAAAATCTCTCAAAGATCTCAATCTTTCAGGTTGCTCAAAGCTTGACAAATTGCCGGATGAGTTGGGTGATGTTGCCTGTTTGGAGAAGCTCGATGTGAGTGGAAGTGGCATAAGAGAACTGCCCTCCTCTATTGGTCTTTTGAAAAACCTCAAAGAATTCTCTCTTGCTGGGTGTAAAGCAGAGTCACCTAAAACATGGAATATAATCCCCTTTCAGTTATTGCGAAAAAGAAGTCACATTCTGGCAGGATTGTCGTTGCCTTGTTTATCTGGTTTGCATTCATTAACTAAATTGAATCTAAGTGACTGCAATCTTTCTGAAGAAGCAATGCCCAGTGATTTTGGATGCTTGTCTTCATTAAGAGAATTAAATCTCAGCAGAAATCAATTCAGTAGACTACCCGAGAGCATCGGCCAACTCTCTAGACTTGACTATCTTCACTTGGAATGGTGCCAGAAGCTTCGGAGATTACCAGAGCTTCCATCTCATGTGTGGGTAGACGCTGACAACTGCATTTCATTAGATACATTGGCCAATCAAAGCAATTCGTTGGGACAAGGATATTTTGTTAACTGTTTCAAACTAGTGGAGAATCAAAGTTGTGAGAGTATAGCACTTTCATTGCTAACACGATGCCTTAAGAGTCCAGCCAATAAAGAATTTGGCTTTGTTGTTCCTGGAAATGAAATTCCAGAGTGGTACAATCACCGAAGTGTGGGGTCTTCAATAACCGTAGAGCTGCATCCAGGTTGGTTTAGTAACAAGTGGATGGGATTCGCCTGCTGTGTCGTTTTTAGACCCCTCAAACCACTCCCGCCTTTGGTTCACTGGCACATTAAATGCGAACTGAGTGCCAATGGAGAATCCTTGCATCACTTTAGGAGTTTGGAGTTTGGGGGAGAGTGGGGTCAACCTGTGTTGGGTCACATTTGGTTGTCGTATGTGCACAGAGATGGATACTCCTCCCAACATAAGTGGCAGGATATTTACACTCAGCTTGTATTCTCATTTAAAGCAGGTGGAAATGAAATCAATGAGGAAAATGTGCAAGTGAAGAAGTGTGGGGTCCGTATGATATATGAGGAAGATGCAGAGGAGCTTTCGGAAACATTATTGAAGCAGAGGAATATTGGCATTAATACCAAGCAATGCCTTCAACACAATGACGGTGATGAAGCATCTAGTAGTGCAAGTCAGGCTCATCCAAAAAGAATTAAACAAACTCCACCTTGA

Protein Analysis

482

Amino Acids

53.57

Weight (kDa)

6.55

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 9 - 87 1.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 14 - 81 2.6e-10 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 20 - 83 5e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 57 - 128 1.8e-09 Leucine-rich repeat region
LRR_13 PF23286 87 - 267 4e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 157 - 231 3.1e-08 Leucine-rich repeat region
LRR_8 PF13855 186 - 224 8e-06 Leucine rich repeat
C-JID PF20160 297 - 437 2e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1223
Acc36I ACCTGC 1 cut(s) 1223
AccB1I GGYRCC 1 cut(s) 666
AccI GTMKAC 2 cut(s) 610, 714
AciI CCGC 1 cut(s) 1031
AcoI YGGCCR 2 cut(s) 628, 747
AcsI RAATTY 3 cut(s) 371, 875, 903
AcuI CTGAAG 1 cut(s) 555
AfaI GTAC 1 cut(s) 917
AfiI CCNNNNNNNGG 3 cut(s) 931, 1036, 1136
AflII CTTAAG 1 cut(s) 854
AgsI TTSAA 7 cut(s) 146, 358, 511, 796, 942, 1333, 1373
AhlI ACTAGT 1 cut(s) 799
AjnI CCWGG 2 cut(s) 892, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AleI CACNNNNGTG 1 cut(s) 927
AluBI AGCT 8 cut(s) 43, 256, 304, 676, 694, 955, 1213, 1315
AluI AGCT 8 cut(s) 43, 256, 304, 676, 694, 955, 1213, 1315
Alw21I GWGCWC 1 cut(s) 1164
Alw44I GTGCAC 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 2 cut(s) 628, 747
ApaLI GTGCAC 1 cut(s) 1160
ApeKI GCWGC 1 cut(s) 955
ApoI RAATTY 3 cut(s) 371, 875, 903
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AseI ATTAAT 1 cut(s) 1353
AspS9I GGNCC 1 cut(s) 1282
AsuHPI GGTGA 4 cut(s) 293, 393, 914, 1397
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 1282
BaeGI GKGCMC 1 cut(s) 1164
BalI TGGCCA 1 cut(s) 749
BanI GGYRCC 1 cut(s) 666
BbsI GAAGAC 3 cut(s) 185, 561, 930
Bbv12I GWGCWC 1 cut(s) 1164
BbvI GCAGC 1 cut(s) 942
BccI CCATC 3 cut(s) 706, 979, 1163
BcgI CGANNNNNNTGC 4 cut(s) 450, 484, 1049, 1083
BciT130I CCWGG 2 cut(s) 894, 963
BciVI GTATCC 1 cut(s) 1166
BcuI ACTAGT 1 cut(s) 799
BfaI CTAG 3 cut(s) 639, 800, 1398
BfrI CTTAAG 1 cut(s) 854
BfuAI ACCTGC 1 cut(s) 1223
BfuI GTATCC 1 cut(s) 1166
BglII AGATCT 1 cut(s) 229
BisI GCNGC 1 cut(s) 956
BlsI GCNGC 1 cut(s) 957
Bme1390I CCNGG 2 cut(s) 894, 963
Bme18I GGWCC 1 cut(s) 1282
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 1282
BmiI GGNNCC 2 cut(s) 668, 1283
BmrFI CCNGG 2 cut(s) 894, 963
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 8 cut(s) 31, 551, 633, 839, 967, 1096, 1294, 1403
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 185, 561, 930
BsaBI GATNNNNATC 1 cut(s) 234
Bsc4I CCNNNNNNNGG 3 cut(s) 931, 1036, 1136
Bse1I ACTGG 2 cut(s) 549, 1049
Bse3DI GCAATG 3 cut(s) 549, 836, 1370
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 2 cut(s) 894, 963
BseGI GGATG 6 cut(s) 64, 277, 566, 958, 990, 1417
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 3 cut(s) 931, 1036, 1136
BseMI GCAATG 3 cut(s) 549, 836, 1370
BseMII CTCAG 3 cut(s) 604, 1055, 1223
BseNI ACTGG 2 cut(s) 549, 1049
BseRI GAGGAG 4 cut(s) 118, 331, 1168, 1325
BseSI GKGCMC 1 cut(s) 1164
BseXI GCAGC 1 cut(s) 942
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 2 cut(s) 630, 749
BshNI GGYRCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 1164
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 2 cut(s) 56, 269
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 3 cut(s) 931, 1036, 1136
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 2 cut(s) 630, 749
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 1164
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 1 cut(s) 1031
BspANI GGCC 2 cut(s) 630, 749
BspCNI CTCAG 3 cut(s) 603, 1056, 1222
BspLI GGNNCC 2 cut(s) 668, 1283
BspMI ACCTGC 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 666
BspTI CTTAAG 1 cut(s) 854
BsrDI GCAATG 3 cut(s) 549, 836, 1370
BsrI ACTGG 2 cut(s) 549, 1049
BssMI GATC 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 894, 963
Bst4CI ACNGT 4 cut(s) 191, 791, 949, 1385
BstAFI CTTAAG 1 cut(s) 854
BstC8I GCNNGC 2 cut(s) 140, 997
BstDEI CTNAG 4 cut(s) 515, 590, 1064, 1209
BstF5I GGATG 6 cut(s) 64, 277, 566, 958, 990, 1417
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 1 cut(s) 1412
BstNI CCWGG 2 cut(s) 894, 963
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 892, 961
BstSLI GKGCMC 1 cut(s) 1164
BstV1I GCAGC 1 cut(s) 942
BstV2I GAAGAC 3 cut(s) 185, 561, 930
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuI GTATCC 1 cut(s) 1166
BsuRI GGCC 2 cut(s) 630, 749
BtsCI GGATG 6 cut(s) 64, 277, 566, 958, 990, 1417
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 4 cut(s) 187, 208, 556, 1042
BveI ACCTGC 1 cut(s) 1223
Cac8I GCNNGC 2 cut(s) 140, 997
Cfr13I GGNCC 1 cut(s) 1282
CseI GACGC 1 cut(s) 725
Csp6I GTAC 1 cut(s) 916
CspCI CAANNNNNGTGG 2 cut(s) 1172, 1207
CviAII CATG 4 cut(s) 71, 139, 411, 704
CviQI GTAC 1 cut(s) 916
DdeI CTNAG 4 cut(s) 515, 590, 1064, 1209
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
DraI TTTAAA 1 cut(s) 1228
EaeI YGGCCR 2 cut(s) 628, 747
Eco47I GGWCC 1 cut(s) 1282
Eco57I CTGAAG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 892, 961
FaeI CATG 4 cut(s) 74, 142, 414, 707
FaqI GGGAC 1 cut(s) 783
FatI CATG 4 cut(s) 70, 138, 410, 703
FauI CCCGC 1 cut(s) 1038
FblI GTMKAC 2 cut(s) 610, 714
Fnu4HI GCNGC 1 cut(s) 956
FokI GGATG 6 cut(s) 71, 284, 573, 945, 997, 1404
Fsp4HI GCNGC 1 cut(s) 956
FspBI CTAG 3 cut(s) 639, 800, 1398
GluI GCNGC 1 cut(s) 956
GsaI CCCAGC 1 cut(s) 387
HaeIII GGCC 2 cut(s) 630, 749
HapII CCGG 2 cut(s) 56, 269
HgaI GACGC 1 cut(s) 725
Hin1II CATG 4 cut(s) 74, 142, 414, 707
HincII GTYRAC 2 cut(s) 787, 1127
HindII GTYRAC 2 cut(s) 787, 1127
HindIII AAGCTT 3 cut(s) 41, 254, 674
HinfI GANTC 7 cut(s) 88, 398, 511, 808, 859, 990, 1079
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 2 cut(s) 56, 269
HphI GGTGA 4 cut(s) 293, 393, 914, 1397
Hpy166II GTNNAC 6 cut(s) 611, 715, 787, 1042, 1127, 1162
Hpy188I TCNGA 3 cut(s) 535, 681, 1321
Hpy188III TCNNGA 3 cut(s) 92, 639, 908
Hpy8I GTNNAC 6 cut(s) 611, 715, 787, 1042, 1127, 1162
HpyAV CCTTC 2 cut(s) 140, 1379
HpyCH4III ACNGT 4 cut(s) 191, 791, 949, 1385
HpyF10VI GCNNNNNNNGC 1 cut(s) 1412
HpyF3I CTNAG 4 cut(s) 515, 590, 1064, 1209
Hsp92II CATG 4 cut(s) 74, 142, 414, 707
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 1 cut(s) 1312
Lsp1109I GCAGC 1 cut(s) 942
LweI GCATC 8 cut(s) 31, 551, 633, 839, 967, 1096, 1294, 1403
MaeI CTAG 3 cut(s) 639, 800, 1398
MaeIII GTNAC 5 cut(s) 399, 449, 518, 974, 1139
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 8 cut(s) 176, 185, 548, 561, 644, 930, 1282, 1313
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 1164
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 9 cut(s) 263, 371, 506, 581, 602, 760, 875, 903, 1428
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 2 cut(s) 407, 868
Mox20I TGGCCA 1 cut(s) 749
MscI TGGCCA 1 cut(s) 749
MslI CAYNNNNRTG 2 cut(s) 927, 1167
Msp20I TGGCCA 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 854
MspI CCGG 2 cut(s) 56, 269
MspR9I CCNGG 2 cut(s) 894, 963
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 2 cut(s) 894, 963
MwoI GCNNNNNNNGC 1 cut(s) 1412
NdeII GATC 1 cut(s) 229
NlaIII CATG 4 cut(s) 74, 142, 414, 707
NlaIV GGNNCC 2 cut(s) 668, 1283
NmuCI GTSAC 4 cut(s) 399, 449, 518, 1139
NspI RCATGY 1 cut(s) 142
OliI CACNNNNGTG 1 cut(s) 927
PaeI GCATGC 1 cut(s) 142
PaqCI CACCTGC 1 cut(s) 1223
PctI GAATGC 1 cut(s) 493
PfeI GAWTC 5 cut(s) 88, 511, 808, 990, 1079
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 1 cut(s) 957
PleI GAGTC 2 cut(s) 406, 867
PpsI GAGTC 2 cut(s) 406, 867
PshBI ATTAAT 1 cut(s) 1353
Psp6I CCWGG 2 cut(s) 892, 961
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 892, 961
PspN4I GGNNCC 2 cut(s) 668, 1283
PspPI GGNCC 1 cut(s) 1282
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 917
RsaNI GTAC 1 cut(s) 916
RseI CAYNNNNRTG 2 cut(s) 927, 1167
SatI GCNGC 1 cut(s) 956
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 1282
SchI GAGTC 2 cut(s) 407, 868
ScrFI CCNGG 2 cut(s) 894, 963
SduI GDGCHC 1 cut(s) 1164
SfaNI GCATC 8 cut(s) 31, 551, 633, 839, 967, 1096, 1294, 1403
SinI GGWCC 1 cut(s) 1282
SmiMI CAYNNNNRTG 2 cut(s) 927, 1167
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SpeI ACTAGT 1 cut(s) 799
SphI GCATGC 1 cut(s) 142
Sse9I AATT 9 cut(s) 263, 371, 506, 581, 602, 760, 875, 903, 1428
SsiI CCGC 1 cut(s) 1031
SspI AATATT 1 cut(s) 1345
SspMI CTAG 3 cut(s) 639, 800, 1398
StyD4I CCNGG 2 cut(s) 892, 961
TaaI ACNGT 4 cut(s) 191, 791, 949, 1385
TaqI TCGA 1 cut(s) 306
TasI AATT 9 cut(s) 263, 371, 506, 581, 602, 760, 875, 903, 1428
TfiI GAWTC 5 cut(s) 88, 511, 808, 990, 1079
TscAI CASTG 4 cut(s) 194, 208, 556, 1049
TseFI GTSAC 4 cut(s) 399, 449, 518, 1139
TseI GCWGC 1 cut(s) 955
Tsp45I GTSAC 4 cut(s) 399, 449, 518, 1139
TspDTI ATGAA 7 cut(s) 486, 561, 723, 825, 915, 1257, 1404
TspGWI ACGGA 1 cut(s) 1274
TspRI CASTG 4 cut(s) 194, 208, 556, 1049
Vha464I CTTAAG 1 cut(s) 854
VneI GTGCAC 1 cut(s) 1160
VpaK11BI GGWCC 1 cut(s) 1282
VspI ATTAAT 1 cut(s) 1353
XapI RAATTY 3 cut(s) 371, 875, 903
XbaI TCTAGA 1 cut(s) 638
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XmiI GTMKAC 2 cut(s) 610, 714
XspI CTAG 3 cut(s) 639, 800, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.