Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003667

Physical Location & Seq

Genomic Coordinates Forward (+)

22790527 .. 22799138

Gene Structure 8612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G012500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGGGGAAGAAGAAAAGCAGAGATGGGAGCCATACCAGAACCGAAACCGAGCACTGTACCTCCACCGTCTTCGTCTCCAATTTGCCCTACTCTTTCACCAATTCCCAGCTGGAGGAGACGTTCAGTGAAGTCGGACCGATCAGGCGGTGCTTTATGGTCACAAAAAAGGGGTCGGCGGAGCACCGTGGTTTTGGCTTTGTTCAATTTGCTGTCACAACTGATGCTGATCGCTCCGTTGAGCTGAAGAATGGTGTGTCTGTTGGAGGTCGAAAGATTGCTGTTAAGCATGCCATGCACCGTGCACCTTTTGAACAACGAAGATCAAAGAAAGATCAAGAGATTAAATTGGATAAGACCATGAAGTCAAAGAATGATAAGGATGCTGGCAATTTGAAAAAGGAAAAGGATGCTTCAAAGTTGGAGGAAAAAGAAAAAACTGTGAAACCCAGAAAACCAGTAGCACCATGTAAAGATGTTGTGGCAAAAGTGGGAGGTTCTGAAAAGCAGAGGGTTGCTAGGACTGTTATATTTGGTTGCCTTCTCAATGCTGGCATGGCGGAAGATGTTCATCGCCAAGCTAGGGAGGTTGGCGATGTGTGTTCTATAACTTATCCTTTTCCCAAAGAAGAGCTTCAACAACATGGTCTTGTACAAGATGGATGCAAAATGGATGCCTCAGCTGTACTTTACAACAGTGTTAAATCAGCTCATGCTTCTGTTGCAATGTTACACCAAAAGGAGATAAAAGGAGGAATTGTTTGGGCCCGTCAGCTGGGTGGGGAGGGTTCCAAGACTCGGAAGTGGAAGCTCATTGTCAGAAATCTTCCTTTCAAGGCCAAAGTCAGTGATATCAAAGATATGTTTTCATCTGCGGGATTTGTCTGGGAGGTTTATATACCACAGAATTCTGATACAGGGTTGTCTAAAGGATTTGCATTCGTCAAATTCACACGCAGACAAGATGCGGAAAATGCAATAAAAAAGTTCAATGGACAAAAACTTCTTAAAAGACCAATTGCTGTGGACTGGGCTGTTCCTAAGCAGATATATGGTAGTGGCAATGACACCCTTGCTTCAGAAGATGGAGAAAAGGGCGGAAGAGATGGGGGAAATGATAGTAGCAGTGATGATTTTGAGGGTGATGCTGGAGATGCATGTAAAAAGTCCCAGCACCTTGATGGGATTGACAGTGCTTCAGATGACTCCAATACCACAGAGCTGAAAGACATCCCTACTGAAATTGATTTTGAGGAGGAAGCAGACATAGCAAGAAAAGTTCTAAAAAACTTGACCACGCCCTCTGGTACAGTAACTGCTGTTGATAATTCTGCATTGCCCAAAGGTAACAAGGAGCCAACTATTGATTTTGAGGAGGAAGCAGACATTGCAAGAAAAGTTCTAAAAAAGTTTACCACACCCTCTGGTACAGTAACTACTGTTGATGATTCTGCACTGCCCGTAAACAACAAGGAGCCAACTATTGAAGAATCTGCTAATGAGACTAGTAAGTTATCTTCCGAGACTGCGCCTAATAAATTATCTTCTGAAAGTGCCAGTGCATCAGATGTTGCTAAACCACAAACGGAAGAAGTAGATGATTTGCATAGAACAATTTTCATCAGCAACCTTCCTTTTGAGATCACTAATGAAGAAGTCAAACAAAGGTTTTCTGCTTTCGGGCAAGTGCAATCTTTTGTCCCAGTTCTTCATCCACTTACCAAGCGACCAAAAGGAACTGGCTTTCTCAAGTTTAAGACAATAGATGCAGTGAATTCTGCTGTTTCAGCTGGGAGTGCCGCATCTGGCTTGGGGATTTTTCTCAAGGGTAGACAACTGACAGTCTTCCAGGCTTTGGATAGAAAATCAGCTCATGAGAAGGAAGAAAATATGGCCAAAAAAGAGGACATAGACCACCGCAATCTCTATTTGGCAAAGGAAGGTCTTATTCTTGCGGGAACTCCCGCTGCAGAAGGGGTTTCAGCTAATGATATGTCAAAACGCCAAATGTTGGAGAGGAGCAAGGCGATGAAGCTTAAATCTCCAAATTTTCATGTTTCAAAGACAAGATTAGTTATGTACAATTTGCCAAAGTCCATGACTGAAAAACAGCTTAAGAAACTTTGTATGGATGCTGTTACCTCACGAGCGAAGCTACAAAAACCTGTGATTCGACAGATAAAGTGCTTGAAGGATGTGAAAAAGGGAAAGATTGTTGCAAAGAACCACTCTCGCGGTGTTGCTTTCATTGAGTTTACAGAGCACCAGCATGCCCTTGTGGCTCTGAGAGTTCTTAACAACAATCCTGAAACTTTTGGTTCAGAACATCGGCCAATTGTGGAGTTTGCACTTGATAATGTTCAGAAACTGAGAGCACGTCAGGTATCACAACAAACTCAGCAGCATGCCGCTAATGGTAATCAGAAAGAGGGGCGGCAATTTGATCACTCAAATAGAGCAGATGCCCACCCAAGCAAGAAGTTTGAAAACCGGAGAGACAAAGGTGACAAGCGAAAACTGGATGAGGTAGTACCAAATAAAGAGGGTGAAGTGGAAAATAGGGCAAGTGATGGAGCTGCCACTGAAGGGCAGAGATTTCCCAAGAGGCAGAAGAATGGTTCAGATAGAAAGGCAGAAAATATTTCATCAAAAAAAGTACTGGGAGGCTCATCGGTAGAAGGAGGCTGGCGGTGGAGATCGTATGCAGGTGAAATCAAGGCAGTTGATGCGCCCACATCAATATCTTCGAGAAAGACAAATGTGGAACCTAAGAAGAGGAAGCTGCAGGAGCAAAATTACACGGAGGGAGGAGAAAACATGATGAGAAGAAGGCCGAAGAATAAAGACCCAACAGGGCGGGATGTTACAGATAAACTTGACATGCTGATTGAAAAATATAGATCCACATTCTCAAATCAGAATTCCTTCCAAAAAGGTGGCGGAAGGCAAGATTCAATTAGAAAATGGATTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RRM_1

Protein Analysis

990

Amino Acids

109.87

Weight (kDa)

9.46

Isoelectric Point (pI)

39.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	23 - 92	2.3e-16	RNA recognition motif
RRM_1	PF00076	270 - 339	6.4e-16	RNA recognition motif
RRM_1	PF00076	538 - 595	1.1e-12	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003667)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21440
fragaria_vesca	FvH4_2g09800 FvH4_2g09800 FvH4_2g09800 FvH4_2g09800 FvH4_2g09800 FvH4_7g06401 FvH4_7g06402 FvH4_7g06403 FvH4_7g12280 FvH4_7g12280 FvH4_7g12280 FvH4_7g12280 FvH4_7g12280
malus_domestica	MD05G1059700.v1.1 MD10G1067900.v1.1
prunus_persica	Prupe.8G085300_v2.0.a1 Prupe.8G089600_v2.0.a1 Prupe.8G089700_v2.0.a1 Prupe.8G095100_v2.0.a1
pyrus_communis	pycom05g05100 pycom10g05370
rosa_chinensis	RchiOBHm_Chr6g0266121
rosa_laevigata	RLG00000014114
rosa_multiflora	Rmu_sc0004324.1_g000009 Rmu_sc0022087.1_g000001 Rmu_sc0040448.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435210 Rroxscaffold_7G00201960
rosa_rugosa	Rorug06G0024200
rosa_samantha	Rh6AG144800 Rh6BG145400 Rh6CG141900 Rh6DG129700
rosa_wichuraiana	Rw6G012500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2693
AasI	GACNNNNNNGTC	1 cut(s)	361
Acc36I	ACCTGC	1 cut(s)	2693
AccB7I	CCANNNNNTGG	2 cut(s)	1852, 2008
AccI	GTMKAC	1 cut(s)	1828
AccII	CGCG	1 cut(s)	2232
AclWI	GGATC	1 cut(s)	2892
AcoI	YGGCCR	2 cut(s)	1890, 2327
AcsI	RAATTY	5 cut(s)	904, 944, 1771, 2044, 2917
AcuI	CTGAAG	4 cut(s)	263, 1059, 1179, 2601
AfaI	GTAC	8 cut(s)	58, 651, 684, 1306, 1426, 2078, 2529, 2655
AfiI	CCNNNNNNNGG	7 cut(s)	112, 580, 772, 795, 1852, 2008, 2583
AflII	CTTAAG	1 cut(s)	2111
AhlI	ACTAGT	1 cut(s)	1502
AjiI	CACGTC	1 cut(s)	2375
AjnI	CCWGG	1 cut(s)	1845
AloI	GAACNNNNNNTCC	2 cut(s)	104, 136
Alw21I	GWGCWC	5 cut(s)	54, 183, 304, 2262, 2374
Alw26I	GTCTC	5 cut(s)	79, 110, 1493, 1514, 2487
Alw44I	GTGCAC	1 cut(s)	300
AlwI	GGATC	1 cut(s)	2892
AlwNI	CAGNNNCTG	2 cut(s)	1313, 2365
AoxI	GGCC	5 cut(s)	762, 834, 1890, 2327, 2828
ApaI	GGGCCC	1 cut(s)	766
ApaLI	GTGCAC	1 cut(s)	300
ApeKI	GCWGC	4 cut(s)	1964, 2398, 2573, 2779
ApoI	RAATTY	5 cut(s)	904, 944, 1771, 2044, 2917
ArsI	GACNNNNNNTTYG	2 cut(s)	155, 187
Asp700I	GAANNNNTTC	3 cut(s)	564, 630, 2921
AspLEI	GCGC	2 cut(s)	1528, 2728
AspS9I	GGNCC	3 cut(s)	134, 762, 763
AsuHPI	GGTGA	5 cut(s)	88, 1151, 2513, 2555, 2717
AvaII	GGWCC	1 cut(s)	134
BaeGI	GKGCMC	2 cut(s)	304, 766
BalI	TGGCCA	1 cut(s)	1892
BanII	GRGCYC	1 cut(s)	766
BarI	GAAGNNNNNNTAC	2 cut(s)	1498, 1530
BauI	CACGAG	1 cut(s)	2142
BbsI	GAAGAC	2 cut(s)	61, 1834
Bbv12I	GWGCWC	5 cut(s)	54, 183, 304, 2262, 2374
BbvCI	CCTCAGC	1 cut(s)	676
BbvI	GCAGC	4 cut(s)	1951, 2410, 2560, 2766
BccI	CCATC	6 cut(s)	17, 650, 1076, 1097, 1172, 2561
BciT130I	CCWGG	1 cut(s)	1847
BclI	TGATCA	1 cut(s)	2440
BcoDI	GTCTC	5 cut(s)	79, 110, 1493, 1514, 2487
BcuI	ACTAGT	1 cut(s)	1502
BfaI	CTAG	3 cut(s)	516, 579, 1503
BfmI	CTRYAG	2 cut(s)	1965, 2780
BfrI	CTTAAG	1 cut(s)	2111
BfuAI	ACCTGC	1 cut(s)	2693
BglI	GCCNNNNNGGC	1 cut(s)	2276
BisI	GCNGC	7 cut(s)	1797, 1965, 2399, 2406, 2432, 2574, 2780
BlsI	GCNGC	7 cut(s)	1798, 1966, 2400, 2407, 2433, 2575, 2781
BmcAI	AGTACT	1 cut(s)	2655
Bme1390I	CCNGG	1 cut(s)	1847
Bme18I	GGWCC	1 cut(s)	134
BmgBI	CACGTC	1 cut(s)	2375
BmgT120I	GGNCC	3 cut(s)	134, 762, 763
BmiI	GGNNCC	6 cut(s)	29, 764, 787, 1353, 1473, 2763
BmrFI	CCNGG	1 cut(s)	1847
BmrI	ACTGGG	3 cut(s)	1036, 1694, 2666
BmuI	ACTGGG	3 cut(s)	1036, 1694, 2666
BpiI	GAAGAC	2 cut(s)	61, 1834
BpmI	CTGGAG	2 cut(s)	131, 1167
Bpu10I	CCTNAGC	2 cut(s)	676, 1038
BpuEI	CTTGAG	2 cut(s)	1730, 1805
BsaBI	GATNNNNATC	2 cut(s)	225, 567
BsaJI	CCNNGG	1 cut(s)	184
BsaWI	WCCGGW	1 cut(s)	2487
BsaXI	ACNNNNNCTCC	6 cut(s)	44, 74, 104, 134, 741, 771
Bsc4I	CCNNNNNNNGG	7 cut(s)	112, 580, 772, 795, 1852, 2008, 2583
Bse1I	ACTGG	7 cut(s)	455, 1031, 1554, 1700, 1741, 2520, 2661
Bse3DI	GCAATG	4 cut(s)	729, 1066, 1331, 1383
Bse8I	GATNNNNATC	2 cut(s)	225, 567
BseBI	CCWGG	1 cut(s)	1847
BseDI	CCNNGG	1 cut(s)	184
BseJI	GATNNNNATC	2 cut(s)	225, 567
BseLI	CCNNNNNNNGG	7 cut(s)	112, 580, 772, 795, 1852, 2008, 2583
BseMI	GCAATG	4 cut(s)	729, 1066, 1331, 1383
BseMII	CTCAG	4 cut(s)	690, 2273, 2357, 2408
BseNI	ACTGG	7 cut(s)	455, 1031, 1554, 1700, 1741, 2520, 2661
BseRI	GAGGAG	5 cut(s)	128, 1265, 1385, 2029, 2820
BseSI	GKGCMC	2 cut(s)	304, 766
BseXI	GCAGC	4 cut(s)	1951, 2410, 2560, 2766
BseYI	CCCAGC	4 cut(s)	105, 772, 1167, 1787
BsgI	GTGCAG	1 cut(s)	1434
Bsh1236I	CGCG	1 cut(s)	2232
BshFI	GGCC	5 cut(s)	764, 836, 1892, 2329, 2830
BsiHKAI	GWGCWC	5 cut(s)	54, 183, 304, 2262, 2374
BsiSI	CCGG	1 cut(s)	2488
BslFI	GGGAC	2 cut(s)	1150, 1682
BslI	CCNNNNNNNGG	7 cut(s)	112, 580, 772, 795, 1852, 2008, 2583
BsmAI	GTCTC	5 cut(s)	79, 110, 1493, 1514, 2487
BsmBI	CGTCTC	2 cut(s)	79, 110
BsmFI	GGGAC	2 cut(s)	1150, 1682
BsmI	GAATGC	1 cut(s)	935
BsnI	GGCC	5 cut(s)	764, 836, 1892, 2329, 2830
Bsp120I	GGGCCC	1 cut(s)	762
Bsp1286I	GDGCHC	6 cut(s)	54, 183, 304, 766, 2262, 2374
Bsp1407I	TGTACA	2 cut(s)	649, 2076
Bsp143I	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
BspANI	GGCC	5 cut(s)	764, 836, 1892, 2329, 2830
BspCNI	CTCAG	4 cut(s)	689, 2274, 2358, 2407
BspFNI	CGCG	1 cut(s)	2232
BspHI	TCATGA	1 cut(s)	1870
BspLI	GGNNCC	6 cut(s)	29, 764, 787, 1353, 1473, 2763
BspMAI	CTGCAG	2 cut(s)	1969, 2784
BspMI	ACCTGC	1 cut(s)	2693
BspPI	GGATC	1 cut(s)	2892
BspQI	GCTCTTC	1 cut(s)	621
BspTI	CTTAAG	1 cut(s)	2111
BsrDI	GCAATG	4 cut(s)	729, 1066, 1331, 1383
BsrGI	TGTACA	2 cut(s)	649, 2076
BsrI	ACTGG	7 cut(s)	455, 1031, 1554, 1700, 1741, 2520, 2661
BssECI	CCNNGG	1 cut(s)	184
BssMI	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
BssSI	CACGAG	1 cut(s)	2142
Bst2BI	CACGAG	1 cut(s)	2142
Bst2UI	CCWGG	1 cut(s)	1847
Bst6I	CTCTTC	3 cut(s)	621, 1093, 2765
BstAFI	CTTAAG	1 cut(s)	2111
BstAPI	GCANNNNNTGC	3 cut(s)	292, 1385, 2723
BstAUI	TGTACA	2 cut(s)	649, 2076
BstC8I	GCNNGC	6 cut(s)	288, 385, 550, 2268, 2403, 2684
BstDEI	CTNAG	6 cut(s)	676, 1038, 2282, 2366, 2394, 2766
BstDSI	CCRYGG	1 cut(s)	184
BstFNI	CGCG	1 cut(s)	2232
BstHHI	GCGC	2 cut(s)	1528, 2728
BstKTI	GATC	8 cut(s)	141, 229, 323, 334, 1641, 2443, 2696, 2900
BstMAI	GTCTC	5 cut(s)	79, 110, 1493, 1514, 2487
BstMBI	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
BstNI	CCWGG	1 cut(s)	1847
BstNSI	RCATGY	5 cut(s)	290, 1158, 2270, 2405, 2881
BstSCI	CCNGG	1 cut(s)	1845
BstSFI	CTRYAG	2 cut(s)	1965, 2780
BstSLI	GKGCMC	2 cut(s)	304, 766
BstUI	CGCG	1 cut(s)	2232
BstV1I	GCAGC	4 cut(s)	1951, 2410, 2560, 2766
BstV2I	GAAGAC	2 cut(s)	61, 1834
BstX2I	RGATCY	1 cut(s)	2897
BstXI	CCANNNNNNTGG	1 cut(s)	2933
BstYI	RGATCY	1 cut(s)	2897
BsuRI	GGCC	5 cut(s)	764, 836, 1892, 2329, 2830
BtgI	CCRYGG	1 cut(s)	184
BtgZI	GCGATG	3 cut(s)	554, 606, 2039
BtrI	CACGTC	1 cut(s)	2375
BtsI	GCAGTG	3 cut(s)	1129, 1451, 1773
BveI	ACCTGC	1 cut(s)	2693
Cac8I	GCNNGC	6 cut(s)	288, 385, 550, 2268, 2403, 2684
CaiI	CAGNNNCTG	2 cut(s)	1313, 2365
CciI	TCATGA	1 cut(s)	1870
CfoI	GCGC	2 cut(s)	1528, 2728
Cfr13I	GGNCC	3 cut(s)	134, 762, 763
CpoI	CGGWCCG	1 cut(s)	134
Csp6I	GTAC	8 cut(s)	57, 650, 683, 1305, 1425, 2077, 2528, 2654
CspCI	CAANNNNNGTGG	2 cut(s)	1002, 1037
CspI	CGGWCCG	1 cut(s)	134
CviQI	GTAC	8 cut(s)	57, 650, 683, 1305, 1425, 2077, 2528, 2654
DdeI	CTNAG	6 cut(s)	676, 1038, 2282, 2366, 2394, 2766
DpnI	GATC	8 cut(s)	140, 228, 322, 333, 1640, 2442, 2695, 2899
DpnII	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
DrdI	GACNNNNNNGTC	1 cut(s)	361
DseDI	GACNNNNNNGTC	1 cut(s)	361
EaeI	YGGCCR	2 cut(s)	1890, 2327
Eam1104I	CTCTTC	3 cut(s)	621, 1093, 2765
EarI	CTCTTC	3 cut(s)	621, 1093, 2765
EciI	GGCGGA	4 cut(s)	191, 572, 1110, 2952
Eco24I	GRGCYC	1 cut(s)	766
Eco32I	GATATC	1 cut(s)	850
Eco47I	GGWCC	1 cut(s)	134
Eco57I	CTGAAG	4 cut(s)	263, 1059, 1179, 2601
EcoRI	GAATTC	3 cut(s)	904, 1771, 2917
EcoRII	CCWGG	1 cut(s)	1845
EcoRV	GATATC	1 cut(s)	850
EcoT22I	ATGCAT	1 cut(s)	1156
EcoT38I	GRGCYC	1 cut(s)	766
Esp3I	CGTCTC	2 cut(s)	79, 110
FalI	AAGNNNNNCTT	2 cut(s)	615, 647
FaqI	GGGAC	2 cut(s)	1150, 1682
FauI	CCCGC	4 cut(s)	865, 1945, 1969, 2847
FbaI	TGATCA	1 cut(s)	2440
FblI	GTMKAC	1 cut(s)	1828
Fnu4HI	GCNGC	7 cut(s)	1797, 1965, 2399, 2406, 2432, 2574, 2780
FriOI	GRGCYC	1 cut(s)	766
Fsp4HI	GCNGC	7 cut(s)	1797, 1965, 2399, 2406, 2432, 2574, 2780
FspBI	CTAG	3 cut(s)	516, 579, 1503
GlaI	GCGC	2 cut(s)	1527, 2727
GluI	GCNGC	7 cut(s)	1797, 1965, 2399, 2406, 2432, 2574, 2780
GsaI	CCCAGC	4 cut(s)	109, 776, 1171, 1791
GsuI	CTGGAG	2 cut(s)	131, 1167
HaeIII	GGCC	5 cut(s)	764, 836, 1892, 2329, 2830
HapII	CCGG	1 cut(s)	2488
HhaI	GCGC	2 cut(s)	1528, 2728
Hin6I	GCGC	2 cut(s)	1526, 2726
HinP1I	GCGC	2 cut(s)	1526, 2726
HindIII	AAGCTT	1 cut(s)	2030
HinfI	GANTC	6 cut(s)	793, 1202, 1445, 1487, 2167, 2948
HpaII	CCGG	1 cut(s)	2488
HphI	GGTGA	5 cut(s)	88, 1151, 2513, 2555, 2717
Hpy166II	GTNNAC	6 cut(s)	302, 1024, 1410, 1462, 1829, 2253
Hpy188III	TCNNGA	5 cut(s)	335, 1871, 2142, 2303, 2745
Hpy8I	GTNNAC	6 cut(s)	302, 1024, 1410, 1462, 1829, 2253
HpyCH4IV	ACGT	2 cut(s)	119, 2374
HpyF3I	CTNAG	6 cut(s)	676, 1038, 2282, 2366, 2394, 2766
HpySE526I	ACGT	2 cut(s)	119, 2374
HspAI	GCGC	2 cut(s)	1526, 2726
Ksp22I	TGATCA	1 cut(s)	2440
Kzo9I	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	8 cut(s)	27, 178, 236, 1351, 1471, 2016, 2570, 2785
Lsp1109I	GCAGC	4 cut(s)	1951, 2410, 2560, 2766
MaeI	CTAG	3 cut(s)	516, 579, 1503
MaeII	ACGT	2 cut(s)	119, 2374
MaeIII	GTNAC	9 cut(s)	157, 211, 726, 1309, 1343, 1429, 2134, 2501, 2860
MalI	GATC	8 cut(s)	140, 228, 322, 333, 1640, 2442, 2695, 2899
MboI	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
MfeI	CAATTG	2 cut(s)	1014, 2331
MflI	RGATCY	1 cut(s)	2897
MhlI	GDGCHC	6 cut(s)	54, 183, 304, 766, 2262, 2374
MlsI	TGGCCA	1 cut(s)	1892
MluNI	TGGCCA	1 cut(s)	1892
MlyI	GAGTC	2 cut(s)	787, 1196
MmeI	TCCRAC	4 cut(s)	112, 241, 399, 1989
Mox20I	TGGCCA	1 cut(s)	1892
Mph1103I	ATGCAT	1 cut(s)	1156
MroXI	GAANNNNTTC	3 cut(s)	564, 630, 2921
MscI	TGGCCA	1 cut(s)	1892
MseI	TTAA	9 cut(s)	282, 342, 699, 1005, 1752, 2034, 2112, 2292, 2971
MslI	CAYNNNNRTG	2 cut(s)	1176, 2265
Msp20I	TGGCCA	1 cut(s)	1892
MspA1I	CMGCKG	5 cut(s)	109, 680, 772, 1787, 1964
MspCI	CTTAAG	1 cut(s)	2111
MspI	CCGG	1 cut(s)	2488
MspR9I	CCNGG	1 cut(s)	1847
MunI	CAATTG	2 cut(s)	1014, 2331
Mva1269I	GAATGC	1 cut(s)	935
MvaI	CCWGG	1 cut(s)	1847
MvnI	CGCG	1 cut(s)	2232
NdeII	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
NlaIV	GGNNCC	6 cut(s)	29, 764, 787, 1353, 1473, 2763
NmuCI	GTSAC	3 cut(s)	157, 211, 2501
NsiI	ATGCAT	1 cut(s)	1156
NspI	RCATGY	5 cut(s)	290, 1158, 2270, 2405, 2881
PaeI	GCATGC	3 cut(s)	290, 2270, 2405
PagI	TCATGA	1 cut(s)	1870
PaqCI	CACCTGC	1 cut(s)	2693
PciSI	GCTCTTC	1 cut(s)	621
PctI	GAATGC	1 cut(s)	935
PdmI	GAANNNNTTC	3 cut(s)	564, 630, 2921
PfeI	GAWTC	4 cut(s)	1445, 1487, 2167, 2948
PflMI	CCANNNNNTGG	2 cut(s)	1852, 2008
PkrI	GCNGC	7 cut(s)	1798, 1966, 2400, 2407, 2433, 2575, 2781
PleI	GAGTC	2 cut(s)	787, 1196
PpsI	GAGTC	2 cut(s)	787, 1196
Psp6I	CCWGG	1 cut(s)	1845
PspFI	CCCAGC	4 cut(s)	105, 772, 1167, 1787
PspGI	CCWGG	1 cut(s)	1845
PspN4I	GGNNCC	6 cut(s)	29, 764, 787, 1353, 1473, 2763
PspOMI	GGGCCC	1 cut(s)	762
PspPI	GGNCC	3 cut(s)	134, 762, 763
PstI	CTGCAG	2 cut(s)	1969, 2784
PstNI	CAGNNNCTG	2 cut(s)	1313, 2365
PsuI	RGATCY	1 cut(s)	2897
PvuII	CAGCTG	4 cut(s)	109, 680, 772, 1787
RsaI	GTAC	8 cut(s)	58, 651, 684, 1306, 1426, 2078, 2529, 2655
RsaNI	GTAC	8 cut(s)	57, 650, 683, 1305, 1425, 2077, 2528, 2654
RseI	CAYNNNNRTG	2 cut(s)	1176, 2265
Rsr2I	CGGWCCG	1 cut(s)	134
RsrII	CGGWCCG	1 cut(s)	134
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	9 cut(s)	282, 342, 699, 1005, 1752, 2034, 2112, 2292, 2971
SatI	GCNGC	7 cut(s)	1797, 1965, 2399, 2406, 2432, 2574, 2780
Sau3AI	GATC	8 cut(s)	138, 226, 320, 331, 1638, 2440, 2693, 2897
Sau96I	GGNCC	3 cut(s)	134, 762, 763
ScaI	AGTACT	1 cut(s)	2655
SchI	GAGTC	2 cut(s)	787, 1196
ScrFI	CCNGG	1 cut(s)	1847
SduI	GDGCHC	6 cut(s)	54, 183, 304, 766, 2262, 2374
SfcI	CTRYAG	2 cut(s)	1965, 2780
SinI	GGWCC	1 cut(s)	134
SmiMI	CAYNNNNRTG	2 cut(s)	1176, 2265
SmlI	CTYRAG	3 cut(s)	1745, 1820, 2111
SmoI	CTYRAG	3 cut(s)	1745, 1820, 2111
SpeI	ACTAGT	1 cut(s)	1502
SphI	GCATGC	3 cut(s)	290, 2270, 2405
SspI	AATATT	1 cut(s)	2638
SspMI	CTAG	3 cut(s)	516, 579, 1503
StyD4I	CCNGG	1 cut(s)	1845
TaiI	ACGT	2 cut(s)	122, 2377
TaqI	TCGA	3 cut(s)	268, 2170, 2744
TaqII	GACCGA	1 cut(s)	151
TatI	WGTACW	4 cut(s)	649, 682, 2076, 2653
TauI	GCSGC	3 cut(s)	1799, 2408, 2434
TfiI	GAWTC	4 cut(s)	1445, 1487, 2167, 2948
Tru1I	TTAA	9 cut(s)	282, 342, 699, 1005, 1752, 2034, 2112, 2292, 2971
Tru9I	TTAA	9 cut(s)	282, 342, 699, 1005, 1752, 2034, 2112, 2292, 2971
TseFI	GTSAC	3 cut(s)	157, 211, 2501
TseI	GCWGC	4 cut(s)	1964, 2398, 2573, 2779
Tsp45I	GTSAC	3 cut(s)	157, 211, 2501
TspGWI	ACGGA	3 cut(s)	223, 1598, 2813
Van91I	CCANNNNNTGG	2 cut(s)	1852, 2008
Vha464I	CTTAAG	1 cut(s)	2111
VneI	GTGCAC	1 cut(s)	300
VpaK11BI	GGWCC	1 cut(s)	134
XapI	RAATTY	5 cut(s)	904, 944, 1771, 2044, 2917
XceI	RCATGY	5 cut(s)	290, 1158, 2270, 2405, 2881
XcmI	CCANNNNNNNNNTGG	2 cut(s)	106, 1175
XmiI	GTMKAC	1 cut(s)	1828
XmnI	GAANNNNTTC	3 cut(s)	564, 630, 2921
XspI	CTAG	3 cut(s)	516, 579, 1503
ZrmI	AGTACT	1 cut(s)	2655
Zsp2I	ATGCAT	1 cut(s)	1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.