Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003461

Physical Location & Seq

Genomic Coordinates Forward (+)

17774061 .. 17780341

Gene Structure 6281 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0127900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGGCAAGGTGAAGGGAAAGCATCGTCTTGACAAGTACTACCACCTCGCGAAGGAGCACGGCTACCGCTCCCGTGCCTCATGGAAGCTTCTCCAGCTCGACGCCAAGCACTCCTTCCTCCACTCCGCCCACGCCGTCCTCGATCTCTGCGCCGCCCCCGGCGGTTGGATGCAAGTCGCCGTGCAGCGCGTCCCCGTCGGCAGCCTCGTCGTCGGCGTCGACTTGGTCCCCATCGCTCCGGTTCGCGGCGCCCACTCTATCCAGCAGGACATCACGAGGACCGAGTGCGTCGCCAAGCTGAGGAGGCTGATGAGGGACGAAGGCTGCTCTGCCTTCGATTTGGTGCTTCATGATGGCTCGCCCAACGTCGGTGGAGCCTGGTCGTCGGAAGCTACGGCGCAGAACGCCTTGGTTATTGATTCGGTGAAGCTGGCGACGCAGCTCTTGGCTCCCAAAGGCACATTTGTCACCAAGATCTTCAGGTCGCAAGATTATAATGCTGTCTTGTTTTGCCTTAAGGAGTTATTTGAGAAGGTTGAACAGTATAAACCAGCAGCTAGTCGTAGTGCATCTGCCGAGACATATCTTTTGGGTTTGAAGTACAAGGCTCCAGCAAAAATTGATCCTCGCCTACTTGATATGAAGCATCTGTTTAAAGCTGTGGAACCTCAGAAGAAGGTGGTGGATGTACTTAGAGGAACAAAGCAAAAAAGACACCGTGACGGGTATGAAGATGGAGACACAATTTTGAGAAAAGTATCTTCAGCCGCTGACTTCATTTGGTCTGTGGATCCTCTAAATATTCTTGGATCTGTCACTTCCATAACATTTGATGATGTGACATCTTTGCCCATCAAGGATCATGCTTTGACAACAGAGGAGGTTAAAATTCTATGTGATGATCTGCGTGTTTTGGGGAAGCAAGATTTCAAGCATCTTTTGAAGTGGCGGGTGCAGATAAGAAAGGCCTTGTCTCCTTCCGAGAAAGCTGATGTTACTACTGCTCCGGATGTTGAAAAGGAGACTAAGGAGGATGATGATGACAGAATACTGAATGAAATGGAAGAGCTTACAAACGCTATGGAGCGCAAGAAGAAACGGGAAAAGAAGCTTCAAGCAAAGAAACGTGCTCAGGACAAGGTCCGGAAGGCGACTGGGATGCAAATAGATGCAATGCAAGATGGTTATACAGATGATACTCTGTTTTCCCTATCTGCCATCAAGGGTAAGAAAGATCTGGTAGCGATTGATTCCACCGAATATGATGGTGAGAATGGTGACTTGGCAGATAGCGAAAATGAGGATATGCAGGATAAACCTGAGGAAGCATCTTCCAGTGATGTAGACTCTGATGACGGCCGTAGAAGATATGATGAAAAAATGGAAGAACTTCTAGATCAGGCTTATGAACATTATGTGGCCAAAAAGGAGGGAACTGCAAAGCAGCGAAAGCGTTTGAAAGAAGAGGCTCAAAGTTTGGAGGATGTTGATGGTGAAGATACAATTCCATCTGATTATGATTCAGACAAAGATGAGGCTGATCAGGAAAAGAATCCTCTCTTAGATGCTCTTGATGATGGGGAAGGACCAACTCAGGAGGAGGTTACAAATAACTGGTTTAGTCAGCCGATCTTTGCTGAAGCTGTAGAACAGGGAGATTTGGAGAAGTCTGATAGTGAAGACGAAATGCAGGTTGAAAGGCCAGAAGAGAATTTGGCCCTGCAAGAGAAGCCCAAGGAAAAGACGGTTATTAAAAATGTTAAAGAAAAGTCTGAAAATCGTGTTGCAGGGTCCAATTCCAATCATGAACTCAAAGCAAAGAAGGCAGATGATGATTTTGAGATAGTCCCTGCTCCCGATACAGATTCAAGTGATGATTCATCATCAGATGAGTCGGAGGATATGGATACCTATAGAAAAGCCGAGATACTGGCATGTGCCAAAAAAATGTTGTCAAAGAAGCAAAGGGAACATATGCTTGATGATGCGTATAATAAGTACATGCTTGATGACGAGGGCTTGCCCAAATGGTTTTTGGACGAGGAAAAGAAGCACCGACGACCAAATAAGCCAGTGACCAAAGAAGAGATTAATGCAATGAAAGCGCAGTTTAAAGAAATTGATGCCCGGCCAGCAAAGAAGGTGGCAGAGGCTAAAGCTCGAAAGAAGCGGGTTGCCATGAGGAAGCTTGAGAAGATTCGTAAGAAGGCTAACACAATATCAGATCAGGCAGATATCTCTGATCGTTCAAAGAGCAAGCAAATCGACCAATTGTATAAGAAGGCAGTGCCCAAGAGGCCTAAAAAGGAGTATGTGGTTGCGAAGAAAGGTGTTCAAGTCAAGGTTGGCAAGGGTAAGGTCCGTGTTGATGCAAGAATGAAAAAGGATGCAAGGGGTCGTGGGACGGGTAATAAATGGGCAGGAAAAGGCAGTGAAAAGAAAGGAAAAAATATGAAGGACAAGAGAGGTAAAGGTAAAGGATCTGGGAAGCCTTCAGGAGGGAAGCCTTCTGGAAAGAAGGGTGGTAGCAAAGGAAGAAAGATGGGCATGCATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

852

Amino Acids

95.7

Weight (kDa)

8.61

Isoelectric Point (pI)

38.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsJ	PF01728	22 - 201	1.6e-47	FtsJ-like methyltransferase
DUF3381	PF11861	234 - 382	2.2e-45	Ribosomal RNA methyltransferase Spb1, DUF3381
Spb1_C	PF07780	607 - 801	2.9e-60	Spb1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003461)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g16100 FvH4_2g16100 FvH4_2g16100 FvH4_3g37810
pyrus_communis	pycom16g03910
rosa_chinensis	RchiOBHm_Chr5g0011221 RchiOBHm_Chr6g0279771
rosa_laevigata	RLG00000001558 RLG00000013122 RLG00000030029 RLG00000031819
rosa_multiflora	Rmu_co8468977.1_g000001 Rmu_sc0000898.1_g000056 Rmu_sc0001298.1_g000055 Rmu_sc0002990.1_g000006 Rmu_sc0003242.1_g000033
rosa_roxburghii	Rroxscaffold_1G00065180 Rroxscaffold_7G00188680
rosa_rugosa	Rorug01G0480900 Rorug02G0507500 Rorug04G0455700 Rorug04G0455800 Rorug06G0127900 Rorug07G0130900
rosa_samantha	Rh2CG560400 Rh2DG469000 Rh4AG014400 Rh5AG087800 Rh5CG095600 Rh5DG082500 Rh6AG236100 Rh6BG240300 Rh6CG242500 Rh6DG234000
rosa_wichuraiana	Rw5G007600 Rw6G020580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1681
AccB1I	GGYRCC	1 cut(s)	248
AccBSI	CCGCTC	1 cut(s)	69
AccI	GTMKAC	2 cut(s)	219, 1344
AccII	CGCG	3 cut(s)	50, 189, 246
AccIII	TCCGGA	2 cut(s)	1006, 1143
AciI	CCGC	8 cut(s)	67, 126, 153, 162, 246, 768, 949, 2170
AclWI	GGATC	6 cut(s)	617, 785, 798, 817, 867, 2488
AcoI	YGGCCR	3 cut(s)	1357, 1419, 2129
AcsI	RAATTY	2 cut(s)	888, 1711
AcuI	CTGAAG	4 cut(s)	463, 747, 1659, 2478
AcyI	GRCGYC	3 cut(s)	102, 216, 249
AfaI	GTAC	4 cut(s)	38, 602, 690, 2000
AfiI	CCNNNNNNNGG	6 cut(s)	52, 165, 245, 300, 368, 2498
AflII	CTTAAG	1 cut(s)	515
AjnI	CCWGG	1 cut(s)	377
AjuI	GAANNNNNNNTTGG	4 cut(s)	98, 130, 1265, 1297
Alw21I	GWGCWC	2 cut(s)	60, 1132
Alw26I	GTCTC	4 cut(s)	572, 732, 978, 1016
AlwI	GGATC	6 cut(s)	617, 785, 798, 817, 867, 2488
AlwNI	CAGNNNCTG	1 cut(s)	770
Aor13HI	TCCGGA	2 cut(s)	1006, 1143
AoxI	GGCC	7 cut(s)	966, 1357, 1419, 1700, 1716, 2129, 2297
ApeKI	GCWGC	6 cut(s)	184, 201, 324, 439, 554, 1444
ApoI	RAATTY	2 cut(s)	888, 1711
ArsI	GACNNNNNNTTYG	2 cut(s)	571, 603
AseI	ATTAAT	1 cut(s)	2091
Asp700I	GAANNNNTTC	1 cut(s)	1389
AspLEI	GCGC	6 cut(s)	152, 189, 251, 400, 1089, 2107
AspS9I	GGNCC	7 cut(s)	226, 279, 1141, 1586, 1717, 1791, 2359
AsuC2I	CCSGG	2 cut(s)	159, 2128
AsuHPI	GGTGA	6 cut(s)	22, 436, 460, 1280, 1289, 1505
AvaII	GGWCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2359
AxyI	CCTNAGG	1 cut(s)	1320
BaeGI	GKGCMC	1 cut(s)	2292
BalI	TGGCCA	1 cut(s)	1421
BamHI	GGATCC	1 cut(s)	790
BanI	GGYRCC	1 cut(s)	248
BauI	CACGAG	1 cut(s)	274
BbsI	GAAGAC	1 cut(s)	1686
Bbv12I	GWGCWC	2 cut(s)	60, 1132
BbvCI	CCTCAGC	1 cut(s)	299
BbvI	GCAGC	6 cut(s)	196, 213, 311, 451, 566, 1456
BceAI	ACGGC	6 cut(s)	76, 119, 164, 411, 1344, 1372
BcgI	CGANNNNNNTGC	6 cut(s)	190, 224, 1141, 1175, 2245, 2279
BciT130I	CCWGG	1 cut(s)	379
BciVI	GTATCC	1 cut(s)	1900
BclI	TGATCA	1 cut(s)	1540
BcnI	CCSGG	2 cut(s)	159, 2128
BcoDI	GTCTC	4 cut(s)	572, 732, 978, 1016
BfaI	CTAG	2 cut(s)	558, 1394
BfmI	CTRYAG	2 cut(s)	1644, 1912
BfoI	RGCGCY	1 cut(s)	252
BfrI	CTTAAG	1 cut(s)	515
BfuAI	ACCTGC	1 cut(s)	1681
BfuI	GTATCC	1 cut(s)	1900
BglI	GCCNNNNNGGC	1 cut(s)	2296
BglII	AGATCT	2 cut(s)	474, 1234
BisI	GCNGC	9 cut(s)	153, 185, 202, 247, 325, 440, 555, 768, 1445
BlsI	GCNGC	9 cut(s)	154, 186, 203, 248, 326, 441, 556, 769, 1446
BmcAI	AGTACT	1 cut(s)	38
Bme1390I	CCNGG	3 cut(s)	159, 379, 2128
Bme18I	GGWCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2359
BmgT120I	GGNCC	7 cut(s)	226, 279, 1141, 1586, 1717, 1791, 2359
BmiI	GGNNCC	8 cut(s)	228, 250, 376, 450, 609, 666, 792, 1792
BmrFI	CCNGG	3 cut(s)	159, 379, 2128
BmrI	ACTGGG	1 cut(s)	1164
BmuI	ACTGGG	1 cut(s)	1164
BpiI	GAAGAC	1 cut(s)	1686
BpmI	CTGGAG	2 cut(s)	77, 594
Bpu10I	CCTNAGC	2 cut(s)	299, 1131
BpuEI	CTTGAG	1 cut(s)	2210
BpuMI	CCSGG	2 cut(s)	159, 2128
BsaHI	GRCGYC	3 cut(s)	102, 216, 249
BsaJI	CCNNGG	3 cut(s)	157, 408, 1734
BsaWI	WCCGGW	3 cut(s)	238, 1006, 1143
BsaXI	ACNNNNNCTCC	2 cut(s)	988, 1018
Bsc4I	CCNNNNNNNGG	6 cut(s)	52, 165, 245, 300, 368, 2498
Bse1I	ACTGG	5 cut(s)	1159, 1335, 1619, 1935, 2072
Bse21I	CCTNAGG	1 cut(s)	1320
Bse3DI	GCAATG	2 cut(s)	1179, 2103
BseAI	TCCGGA	2 cut(s)	1006, 1143
BseBI	CCWGG	1 cut(s)	379
BseDI	CCNNGG	3 cut(s)	157, 408, 1734
BseGI	GGATG	7 cut(s)	174, 691, 1015, 1039, 1164, 1489, 2392
BseLI	CCNNNNNNNGG	6 cut(s)	52, 165, 245, 300, 368, 2498
BseMI	GCAATG	2 cut(s)	1179, 2103
BseMII	CTCAG	5 cut(s)	290, 683, 1145, 1311, 1607
BseNI	ACTGG	5 cut(s)	1159, 1335, 1619, 1935, 2072
BseRI	GAGGAG	3 cut(s)	316, 893, 1613
BseSI	GKGCMC	1 cut(s)	2292
BseX3I	CGGCCG	1 cut(s)	1357
BseXI	GCAGC	6 cut(s)	196, 213, 311, 451, 566, 1456
BsgI	GTGCAG	2 cut(s)	203, 974
Bsh1236I	CGCG	3 cut(s)	50, 189, 246
Bsh1285I	CGRYCG	1 cut(s)	1360
BshFI	GGCC	7 cut(s)	968, 1359, 1421, 1702, 1718, 2131, 2299
BshNI	GGYRCC	1 cut(s)	248
BsiEI	CGRYCG	1 cut(s)	1360
BsiHKAI	GWGCWC	2 cut(s)	60, 1132
BsiSI	CCGG	5 cut(s)	159, 239, 1007, 1144, 2128
BslFI	GGGAC	5 cut(s)	176, 212, 329, 1832, 2416
BslI	CCNNNNNNNGG	6 cut(s)	52, 165, 245, 300, 368, 2498
BsmAI	GTCTC	4 cut(s)	572, 732, 978, 1016
BsmFI	GGGAC	5 cut(s)	176, 212, 329, 1832, 2416
BsnI	GGCC	7 cut(s)	968, 1359, 1421, 1702, 1718, 2131, 2299
Bsp1286I	GDGCHC	3 cut(s)	60, 1132, 2292
Bsp13I	TCCGGA	2 cut(s)	1006, 1143
Bsp68I	TCGCGA	1 cut(s)	50
BspACI	CCGC	8 cut(s)	67, 126, 153, 162, 246, 768, 949, 2170
BspANI	GGCC	7 cut(s)	968, 1359, 1421, 1702, 1718, 2131, 2299
BspCNI	CTCAG	5 cut(s)	291, 682, 1144, 1312, 1606
BspEI	TCCGGA	2 cut(s)	1006, 1143
BspFNI	CGCG	3 cut(s)	50, 189, 246
BspHI	TCATGA	2 cut(s)	349, 1804
BspLI	GGNNCC	8 cut(s)	228, 250, 376, 450, 609, 666, 792, 1792
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	6 cut(s)	617, 785, 798, 817, 867, 2488
BspQI	GCTCTTC	1 cut(s)	1059
BspT107I	GGYRCC	1 cut(s)	248
BspTI	CTTAAG	1 cut(s)	515
BsrBI	CCGCTC	1 cut(s)	69
BsrDI	GCAATG	2 cut(s)	1179, 2103
BsrI	ACTGG	5 cut(s)	1159, 1335, 1619, 1935, 2072
BssECI	CCNNGG	3 cut(s)	157, 408, 1734
BssNI	GRCGYC	3 cut(s)	102, 216, 249
BssSI	CACGAG	1 cut(s)	274
BssT1I	CCWWGG	2 cut(s)	408, 1734
Bst2BI	CACGAG	1 cut(s)	274
Bst2UI	CCWGG	1 cut(s)	379
Bst4CI	ACNGT	3 cut(s)	543, 719, 1747
Bst6I	CTCTTC	4 cut(s)	1059, 1458, 1701, 2079
BstACI	GRCGYC	3 cut(s)	102, 216, 249
BstAFI	CTTAAG	1 cut(s)	515
BstC8I	GCNNGC	6 cut(s)	359, 432, 2021, 2133, 2258, 2549
BstDEI	CTNAG	8 cut(s)	299, 669, 692, 1026, 1131, 1320, 1561, 1593
BstENI	CCTNNNNNAGG	2 cut(s)	50, 2496
BstF5I	GGATG	7 cut(s)	174, 691, 1015, 1039, 1164, 1489, 2392
BstFNI	CGCG	3 cut(s)	50, 189, 246
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	6 cut(s)	152, 189, 251, 400, 1089, 2107
BstMAI	GTCTC	4 cut(s)	572, 732, 978, 1016
BstMCI	CGRYCG	1 cut(s)	1360
BstNI	CCWGG	1 cut(s)	379
BstNSI	RCATGY	3 cut(s)	1938, 2005, 2551
BstSCI	CCNGG	3 cut(s)	157, 377, 2126
BstSFI	CTRYAG	2 cut(s)	1644, 1912
BstSLI	GKGCMC	1 cut(s)	2292
BstUI	CGCG	3 cut(s)	50, 189, 246
BstV1I	GCAGC	6 cut(s)	196, 213, 311, 451, 566, 1456
BstV2I	GAAGAC	1 cut(s)	1686
BstX2I	RGATCY	5 cut(s)	474, 790, 809, 1234, 2480
BstYI	RGATCY	5 cut(s)	474, 790, 809, 1234, 2480
BstZI	CGGCCG	1 cut(s)	1357
Bsu36I	CCTNAGG	1 cut(s)	1320
BsuI	GTATCC	1 cut(s)	1900
BsuRI	GGCC	7 cut(s)	968, 1359, 1421, 1702, 1718, 2131, 2299
BtgZI	GCGATG	1 cut(s)	217
BtsCI	GGATG	7 cut(s)	174, 691, 1015, 1039, 1164, 1489, 2392
BtsI	GCAGTG	2 cut(s)	2292, 2437
BtsIMutI	CAGTG	4 cut(s)	1342, 2079, 2292, 2437
BtuMI	TCGCGA	1 cut(s)	50
BveI	ACCTGC	1 cut(s)	1681
Cac8I	GCNNGC	6 cut(s)	359, 432, 2021, 2133, 2258, 2549
CaiI	CAGNNNCTG	1 cut(s)	770
CciI	TCATGA	2 cut(s)	349, 1804
CfoI	GCGC	6 cut(s)	152, 189, 251, 400, 1089, 2107
Cfr13I	GGNCC	7 cut(s)	226, 279, 1141, 1586, 1717, 1791, 2359
CseI	GACGC	5 cut(s)	110, 178, 205, 277, 445
Csp6I	GTAC	4 cut(s)	37, 601, 689, 1999
CspCI	CAANNNNNGTGG	4 cut(s)	352, 387, 2124, 2159
CviAII	CATG	9 cut(s)	81, 350, 863, 1805, 1935, 2002, 2179, 2548, 2552
CviQI	GTAC	4 cut(s)	37, 601, 689, 1999
DdeI	CTNAG	8 cut(s)	299, 669, 692, 1026, 1131, 1320, 1561, 1593
DinI	GGCGCC	1 cut(s)	250
DraI	TTTAAA	2 cut(s)	655, 2113
EaeI	YGGCCR	3 cut(s)	1357, 1419, 2129
EagI	CGGCCG	1 cut(s)	1357
Eam1104I	CTCTTC	4 cut(s)	1059, 1458, 1701, 2079
EarI	CTCTTC	4 cut(s)	1059, 1458, 1701, 2079
EciI	GGCGGA	1 cut(s)	115
EclXI	CGGCCG	1 cut(s)	1357
Eco130I	CCWWGG	2 cut(s)	408, 1734
Eco147I	AGGCCT	2 cut(s)	968, 2299
Eco32I	GATATC	1 cut(s)	2236
Eco47I	GGWCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2359
Eco52I	CGGCCG	1 cut(s)	1357
Eco57I	CTGAAG	4 cut(s)	463, 747, 1659, 2478
Eco81I	CCTNAGG	1 cut(s)	1320
EcoNI	CCTNNNNNAGG	2 cut(s)	50, 2496
EcoRII	CCWGG	1 cut(s)	377
EcoRV	GATATC	1 cut(s)	2236
EcoT14I	CCWWGG	2 cut(s)	408, 1734
EcoT22I	ATGCAT	1 cut(s)	2553
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	2 cut(s)	408, 1734
FaeI	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2551, 2555
FalI	AAGNNNNNCTT	2 cut(s)	98, 130
FaqI	GGGAC	5 cut(s)	176, 212, 329, 1832, 2416
FatI	CATG	9 cut(s)	80, 349, 862, 1804, 1934, 2001, 2178, 2547, 2551
FauI	CCCGC	2 cut(s)	942, 2163
FauNDI	CATATG	1 cut(s)	1974
FbaI	TGATCA	1 cut(s)	1540
FblI	GTMKAC	2 cut(s)	219, 1344
Fnu4HI	GCNGC	9 cut(s)	153, 185, 202, 247, 325, 440, 555, 768, 1445
FokI	GGATG	7 cut(s)	181, 698, 1022, 1046, 1171, 1496, 2399
Fsp4HI	GCNGC	9 cut(s)	153, 185, 202, 247, 325, 440, 555, 768, 1445
FspBI	CTAG	2 cut(s)	558, 1394
GlaI	GCGC	6 cut(s)	151, 188, 250, 399, 1088, 2106
GluI	GCNGC	9 cut(s)	153, 185, 202, 247, 325, 440, 555, 768, 1445
GsuI	CTGGAG	2 cut(s)	77, 594
HaeII	RGCGCY	1 cut(s)	252
HaeIII	GGCC	7 cut(s)	968, 1359, 1421, 1702, 1718, 2131, 2299
HapII	CCGG	5 cut(s)	159, 239, 1007, 1144, 2128
HgaI	GACGC	5 cut(s)	110, 178, 205, 277, 445
HhaI	GCGC	6 cut(s)	152, 189, 251, 400, 1089, 2107
Hin1I	GRCGYC	3 cut(s)	102, 216, 249
Hin1II	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2551, 2555
Hin6I	GCGC	6 cut(s)	150, 187, 249, 398, 1087, 2105
HinP1I	GCGC	6 cut(s)	150, 187, 249, 398, 1087, 2105
HincII	GTYRAC	1 cut(s)	220
HindII	GTYRAC	1 cut(s)	220
HindIII	AAGCTT	3 cut(s)	86, 1109, 2186
HinfI	GANTC	9 cut(s)	419, 1250, 1346, 1520, 1552, 1865, 1877, 1892, 2197
HpaII	CCGG	5 cut(s)	159, 239, 1007, 1144, 2128
HphI	GGTGA	6 cut(s)	22, 436, 460, 1280, 1289, 1505
Hpy166II	GTNNAC	2 cut(s)	220, 1345
Hpy8I	GTNNAC	2 cut(s)	220, 1345
HpyCH4III	ACNGT	3 cut(s)	543, 719, 1747
HpyCH4IV	ACGT	2 cut(s)	366, 1126
HpyF3I	CTNAG	8 cut(s)	299, 669, 692, 1026, 1131, 1320, 1561, 1593
HpySE526I	ACGT	2 cut(s)	366, 1126
Hsp92I	GRCGYC	3 cut(s)	102, 216, 249
Hsp92II	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2551, 2555
HspAI	GCGC	6 cut(s)	150, 187, 249, 398, 1087, 2105
KasI	GGCGCC	1 cut(s)	248
Kpn2I	TCCGGA	2 cut(s)	1006, 1143
Ksp22I	TGATCA	1 cut(s)	1540
LguI	GCTCTTC	1 cut(s)	1059
LmnI	GCTCC	9 cut(s)	55, 74, 241, 374, 454, 613, 1009, 1084, 1858
Lsp1109I	GCAGC	6 cut(s)	196, 213, 311, 451, 566, 1456
MaeI	CTAG	2 cut(s)	558, 1394
MaeII	ACGT	2 cut(s)	366, 1126
MaeIII	GTNAC	8 cut(s)	466, 719, 814, 838, 994, 1277, 1603, 2074
MbiI	CCGCTC	1 cut(s)	69
MfeI	CAATTG	1 cut(s)	2270
MflI	RGATCY	5 cut(s)	474, 790, 809, 1234, 2480
MhlI	GDGCHC	3 cut(s)	60, 1132, 2292
MlsI	TGGCCA	1 cut(s)	1421
MluCI	AATT	8 cut(s)	618, 744, 888, 1503, 1711, 1795, 2118, 2270
MluNI	TGGCCA	1 cut(s)	1421
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	1340, 1901
MmeI	TCCRAC	3 cut(s)	146, 366, 1875
Mox20I	TGGCCA	1 cut(s)	1421
Mph1103I	ATGCAT	1 cut(s)	2553
MroI	TCCGGA	2 cut(s)	1006, 1143
MroXI	GAANNNNTTC	1 cut(s)	1389
MscI	TGGCCA	1 cut(s)	1421
MseI	TTAA	7 cut(s)	516, 654, 885, 1752, 1761, 2091, 2112
Msp20I	TGGCCA	1 cut(s)	1421
MspA1I	CMGCKG	1 cut(s)	770
MspCI	CTTAAG	1 cut(s)	515
MspI	CCGG	5 cut(s)	159, 239, 1007, 1144, 2128
MspR9I	CCNGG	3 cut(s)	159, 379, 2128
MunI	CAATTG	1 cut(s)	2270
MvaI	CCWGG	1 cut(s)	379
MvnI	CGCG	3 cut(s)	50, 189, 246
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	2 cut(s)	159, 2128
NdeI	CATATG	1 cut(s)	1974
NlaIII	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2551, 2555
NlaIV	GGNNCC	8 cut(s)	228, 250, 376, 450, 609, 666, 792, 1792
NmeAIII	GCCGAG	2 cut(s)	601, 1948
NmuCI	GTSAC	6 cut(s)	466, 719, 814, 838, 1277, 2074
NruI	TCGCGA	1 cut(s)	50
NsiI	ATGCAT	1 cut(s)	2553
NspI	RCATGY	3 cut(s)	1938, 2005, 2551
PaeI	GCATGC	1 cut(s)	2551
PagI	TCATGA	2 cut(s)	349, 1804
PceI	AGGCCT	2 cut(s)	968, 2299
PciSI	GCTCTTC	1 cut(s)	1059
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 204, 213, 216
PdmI	GAANNNNTTC	1 cut(s)	1389
PfeI	GAWTC	7 cut(s)	419, 1250, 1520, 1552, 1865, 1877, 2197
PflFI	GACNNNGTC	2 cut(s)	224, 1139
PkrI	GCNGC	9 cut(s)	154, 186, 203, 248, 326, 441, 556, 769, 1446
PleI	GAGTC	2 cut(s)	1340, 1900
PluTI	GGCGCC	1 cut(s)	252
PpsI	GAGTC	2 cut(s)	1340, 1900
PshBI	ATTAAT	1 cut(s)	2091
PsiI	TTATAA	1 cut(s)	495
Psp6I	CCWGG	1 cut(s)	377
PspGI	CCWGG	1 cut(s)	377
PspN4I	GGNNCC	8 cut(s)	228, 250, 376, 450, 609, 666, 792, 1792
PspPI	GGNCC	7 cut(s)	226, 279, 1141, 1586, 1717, 1791, 2359
PstNI	CAGNNNCTG	1 cut(s)	770
PsuI	RGATCY	5 cut(s)	474, 790, 809, 1234, 2480
PsyI	GACNNNGTC	2 cut(s)	224, 1139
RruI	TCGCGA	1 cut(s)	50
RsaI	GTAC	4 cut(s)	38, 602, 690, 2000
RsaNI	GTAC	4 cut(s)	37, 601, 689, 1999
SalI	GTCGAC	1 cut(s)	218
SapI	GCTCTTC	1 cut(s)	1059
SaqAI	TTAA	7 cut(s)	516, 654, 885, 1752, 1761, 2091, 2112
SatI	GCNGC	9 cut(s)	153, 185, 202, 247, 325, 440, 555, 768, 1445
Sau96I	GGNCC	7 cut(s)	226, 279, 1141, 1586, 1717, 1791, 2359
ScaI	AGTACT	1 cut(s)	38
SchI	GAGTC	2 cut(s)	1340, 1901
ScrFI	CCNGG	3 cut(s)	159, 379, 2128
SduI	GDGCHC	3 cut(s)	60, 1132, 2292
SfcI	CTRYAG	2 cut(s)	1644, 1912
SfoI	GGCGCC	1 cut(s)	250
SinI	GGWCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2359
SmlI	CTYRAG	2 cut(s)	515, 2189
SmoI	CTYRAG	2 cut(s)	515, 2189
SphI	GCATGC	1 cut(s)	2551
Sse9I	AATT	8 cut(s)	618, 744, 888, 1503, 1711, 1795, 2118, 2270
SseBI	AGGCCT	2 cut(s)	968, 2299
SsiI	CCGC	8 cut(s)	67, 126, 153, 162, 246, 768, 949, 2170
SspDI	GGCGCC	1 cut(s)	248
SspI	AATATT	1 cut(s)	802
SspMI	CTAG	2 cut(s)	558, 1394
StuI	AGGCCT	2 cut(s)	968, 2299
StyD4I	CCNGG	3 cut(s)	157, 377, 2126
StyI	CCWWGG	2 cut(s)	408, 1734
TaaI	ACNGT	3 cut(s)	543, 719, 1747
TaiI	ACGT	2 cut(s)	369, 1129
TaqI	TCGA	6 cut(s)	99, 141, 219, 336, 2161, 2265
TaqII	GACCGA	1 cut(s)	296
TasI	AATT	8 cut(s)	618, 744, 888, 1503, 1711, 1795, 2118, 2270
TatI	WGTACW	4 cut(s)	36, 600, 688, 1998
TauI	GCSGC	3 cut(s)	155, 249, 770
TfiI	GAWTC	7 cut(s)	419, 1250, 1520, 1552, 1865, 1877, 2197
Tru1I	TTAA	7 cut(s)	516, 654, 885, 1752, 1761, 2091, 2112
Tru9I	TTAA	7 cut(s)	516, 654, 885, 1752, 1761, 2091, 2112
TscAI	CASTG	4 cut(s)	1342, 2079, 2292, 2437
TseFI	GTSAC	6 cut(s)	466, 719, 814, 838, 1277, 2074
TseI	GCWGC	6 cut(s)	184, 201, 324, 439, 554, 1444
Tsp45I	GTSAC	6 cut(s)	466, 719, 814, 838, 1277, 2074
TspGWI	ACGGA	1 cut(s)	2351
TspRI	CASTG	4 cut(s)	1342, 2079, 2292, 2437
Tth111I	GACNNNGTC	2 cut(s)	224, 1139
Vha464I	CTTAAG	1 cut(s)	515
VpaK11BI	GGWCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2359
VspI	ATTAAT	1 cut(s)	2091
XagI	CCTNNNNNAGG	2 cut(s)	50, 2496
XapI	RAATTY	2 cut(s)	888, 1711
XbaI	TCTAGA	1 cut(s)	1393
XceI	RCATGY	3 cut(s)	1938, 2005, 2551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2032
XmiI	GTMKAC	2 cut(s)	219, 1344
XmnI	GAANNNNTTC	1 cut(s)	1389
XspI	CTAG	2 cut(s)	558, 1394
ZrmI	AGTACT	1 cut(s)	38
Zsp2I	ATGCAT	1 cut(s)	2553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.