Rh2BG287300

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004215
Physical Location & Seq
Forward (+)
31746660 .. 31754766
8107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG287300.1

Sequence Viewer

Length: 852 bp
ATGCAGCCTGACGGAAACCTTGTTCTGTACCCAGAAAACTCTGAAAACTCTCCGATAGATGCCTACTATGCTACAGATACATATGGGATCTTGGACCTCTATCTCAACAGTACAGATGGCAGTCTTGCTCTAATCAACACCACCAGCTCGGAAGTTTACAGTGTCATCAATTATGATAATCTAGCCAGTAATGATGATCTAGCCAATAACACTGTATATCGGGCAACTGTTGGCATAAACGGAGTATTAAGGGTGTATTCTCATCGGATGGATCCTGACAAAGGCAAACTCAAAGCACCTGTTATTATGTGGTACAAACCAGATGATCCTTGCGATGTTTATGGCCTCTGTGGCCTTAACAGCTATTGCACACTCAATGATGACCAACCAGCCTGTCTTTGCCTTCCTGGATCAGATTACGCTGACCTCAACCGCAGGACTATGGGTTGCTTGAGAAACTATTCAGAACCGGCATGTGCAGCTGGCGGGAAAGAGAATACGACCTTTTATGACATACTCACCATGGAAAATATGGCCTGGGAAGATACTCCTGACTTTAAAGCCACAATGACCATGGAAGAATGCAAGAGGTCTTGTCTAGAGGACTGCAATTGCGGAGCAGCTCTGTTCGACGGGAAAAATTGTATGAAACAGAAGCCACCTCTCAGATATGTGAGAAGAGATGTGCAGCAATCCAACACAGCTTTCTTCAAGGTAAGCAACTTAACAAGTCCTGAAAGCAAGAATAATGGAACTGAAACTAAAGAACCATTTCCGATGACTCCCACTACGAGGGAGGAAGAAAATGCCGCTGGAGATGAGGGGAAAGAGCCACCAGTGACGGTTGGATGA

Protein Analysis

283

Amino Acids

31.36

Weight (kDa)

4.45

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 100 - 137 4.2e-07 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 433, 486, 615, 810
AclWI GGATC 5 cut(s) 95, 266, 279, 320, 418
AfaI GTAC 3 cut(s) 29, 112, 314
AfiI CCNNNNNNNGG 2 cut(s) 281, 792
AgsI TTSAA 1 cut(s) 712
AjnI CCWGG 2 cut(s) 406, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AloI GAACNNNNNNTCC 1 cut(s) 38
AluBI AGCT 5 cut(s) 147, 363, 482, 623, 704
AluI AGCT 5 cut(s) 147, 363, 482, 623, 704
AlwI GGATC 5 cut(s) 95, 266, 279, 320, 418
AoxI GGCC 3 cut(s) 343, 352, 534
ApeKI GCWGC 4 cut(s) 4, 479, 620, 688
Asp700I GAANNNNTTC 2 cut(s) 460, 771
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 511
AvaII GGWCC 1 cut(s) 94
BamHI GGATCC 1 cut(s) 271
BbvI GCAGC 4 cut(s) 16, 491, 632, 700
BccI CCATC 2 cut(s) 110, 262
BciT130I CCWGG 2 cut(s) 408, 538
BfaI CTAG 3 cut(s) 182, 200, 599
BfmI CTRYAG 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 351
BisI GCNGC 5 cut(s) 5, 480, 621, 689, 810
BlsI GCNGC 5 cut(s) 6, 481, 622, 690, 811
Bme1390I CCNGG 2 cut(s) 408, 538
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 2 cut(s) 408, 538
BmsI GCATC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 522, 537, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 792
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 2 cut(s) 186, 836
BseBI CCWGG 2 cut(s) 408, 538
BseDI CCNNGG 3 cut(s) 522, 537, 573
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 281, 792
BseMII CTCAG 1 cut(s) 679
BseNI ACTGG 2 cut(s) 186, 836
BseXI GCAGC 4 cut(s) 16, 491, 632, 700
BsgI GTGCAG 2 cut(s) 498, 707
BshFI GGCC 3 cut(s) 345, 354, 536
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 281, 792
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 3 cut(s) 345, 354, 536
Bsp143I GATC 5 cut(s) 87, 196, 271, 325, 410
Bsp19I CCATGG 2 cut(s) 522, 573
BspACI CCGC 4 cut(s) 433, 486, 615, 810
BspANI GGCC 3 cut(s) 345, 354, 536
BspCNI CTCAG 1 cut(s) 678
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 5 cut(s) 95, 266, 279, 320, 418
BsrFI RCCGGY 1 cut(s) 469
BsrI ACTGG 2 cut(s) 186, 836
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 522, 537, 573
BssMI GATC 5 cut(s) 87, 196, 271, 325, 410
BssT1I CCWWGG 2 cut(s) 522, 573
Bst2UI CCWGG 2 cut(s) 408, 538
Bst4CI ACNGT 5 cut(s) 110, 161, 214, 229, 844
Bst6I CTCTTC 1 cut(s) 673
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 1 cut(s) 665
BstDSI CCRYGG 2 cut(s) 522, 573
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 5 cut(s) 90, 199, 274, 328, 413
BstMBI GATC 5 cut(s) 87, 196, 271, 325, 410
BstMWI GCNNNNNNNGC 4 cut(s) 68, 351, 360, 479
BstNI CCWGG 2 cut(s) 408, 538
BstNSI RCATGY 1 cut(s) 477
BstSCI CCNGG 2 cut(s) 406, 536
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 16, 491, 632, 700
BstX2I RGATCY 2 cut(s) 87, 271
BstYI RGATCY 2 cut(s) 87, 271
BsuRI GGCC 3 cut(s) 345, 354, 536
BtgI CCRYGG 2 cut(s) 522, 573
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 166, 210, 843
Cac8I GCNNGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 94
Csp6I GTAC 3 cut(s) 28, 111, 313
CviAII CATG 3 cut(s) 474, 523, 574
CviQI GTAC 3 cut(s) 28, 111, 313
DdeI CTNAG 1 cut(s) 665
DpnI GATC 5 cut(s) 89, 198, 273, 327, 412
DpnII GATC 5 cut(s) 87, 196, 271, 325, 410
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 673
Eco130I CCWWGG 2 cut(s) 522, 573
Eco47I GGWCC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 406, 536
EcoT14I CCWWGG 2 cut(s) 522, 573
ErhI CCWWGG 2 cut(s) 522, 573
FaeI CATG 3 cut(s) 477, 526, 577
FatI CATG 3 cut(s) 473, 522, 573
FauI CCCGC 1 cut(s) 479
FauNDI CATATG 1 cut(s) 82
Fnu4HI GCNGC 5 cut(s) 5, 480, 621, 689, 810
FokI GGATG 1 cut(s) 280
Fsp4HI GCNGC 5 cut(s) 5, 480, 621, 689, 810
FspBI CTAG 3 cut(s) 182, 200, 599
GluI GCNGC 5 cut(s) 5, 480, 621, 689, 810
GsuI CTGGAG 1 cut(s) 834
HaeIII GGCC 3 cut(s) 345, 354, 536
HapII CCGG 1 cut(s) 470
Hin1II CATG 3 cut(s) 477, 526, 577
HinfI GANTC 1 cut(s) 781
HpaII CCGG 1 cut(s) 470
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 8 cut(s) 43, 54, 151, 267, 415, 466, 668, 777
Hpy188III TCNNGA 4 cut(s) 275, 551, 599, 734
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 5 cut(s) 110, 161, 214, 229, 844
HpyCH4V TGCA 6 cut(s) 4, 369, 479, 585, 609, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 351, 360, 479
HpyF3I CTNAG 1 cut(s) 665
Hsp92II CATG 3 cut(s) 477, 526, 577
Kzo9I GATC 5 cut(s) 87, 196, 271, 325, 410
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 4 cut(s) 16, 491, 632, 700
LweI GCATC 1 cut(s) 49
MaeI CTAG 3 cut(s) 182, 200, 599
MaeIII GTNAC 1 cut(s) 838
MalI GATC 5 cut(s) 89, 198, 273, 327, 412
MboI GATC 5 cut(s) 87, 196, 271, 325, 410
MboII GAAGA 5 cut(s) 554, 590, 690, 700, 812
MfeI CAATTG 1 cut(s) 610
MflI RGATCY 2 cut(s) 87, 271
MluCI AATT 3 cut(s) 169, 610, 640
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 2 cut(s) 720, 826
MnlI CCTC 9 cut(s) 107, 356, 437, 582, 595, 672, 786, 790, 814
MroXI GAANNNNTTC 2 cut(s) 460, 771
MseI TTAA 4 cut(s) 248, 357, 558, 725
MspA1I CMGCKG 2 cut(s) 482, 812
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 408, 538
MunI CAATTG 1 cut(s) 610
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 2 cut(s) 408, 538
MwoI GCNNNNNNNGC 4 cut(s) 68, 351, 360, 479
NcoI CCATGG 2 cut(s) 522, 573
NdeI CATATG 1 cut(s) 82
NdeII GATC 5 cut(s) 87, 196, 271, 325, 410
NlaIII CATG 3 cut(s) 477, 526, 577
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 838
NspI RCATGY 1 cut(s) 477
PctI GAATGC 1 cut(s) 587
PdmI GAANNNNTTC 2 cut(s) 460, 771
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 5 cut(s) 6, 481, 622, 690, 811
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
Psp6I CCWGG 2 cut(s) 406, 536
PspGI CCWGG 2 cut(s) 406, 536
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 2 cut(s) 87, 271
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 29, 112, 314
RsaNI GTAC 3 cut(s) 28, 111, 313
SaqAI TTAA 4 cut(s) 248, 357, 558, 725
SatI GCNGC 5 cut(s) 5, 480, 621, 689, 810
Sau3AI GATC 5 cut(s) 87, 196, 271, 325, 410
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 2 cut(s) 408, 538
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 72
SfiI GGCCNNNNNGGCC 1 cut(s) 351
SinI GGWCC 1 cut(s) 94
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 3 cut(s) 169, 610, 640
SsiI CCGC 4 cut(s) 433, 486, 615, 810
SspMI CTAG 3 cut(s) 182, 200, 599
StyD4I CCNGG 2 cut(s) 406, 536
StyI CCWWGG 2 cut(s) 522, 573
TaaI ACNGT 5 cut(s) 110, 161, 214, 229, 844
TaqI TCGA 1 cut(s) 630
TasI AATT 3 cut(s) 169, 610, 640
TatI WGTACW 1 cut(s) 110
TauI GCSGC 1 cut(s) 812
Tru1I TTAA 4 cut(s) 248, 357, 558, 725
Tru9I TTAA 4 cut(s) 248, 357, 558, 725
TscAI CASTG 3 cut(s) 166, 217, 843
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 4 cut(s) 4, 479, 620, 688
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 1 cut(s) 662
TspGWI ACGGA 2 cut(s) 27, 255
TspRI CASTG 3 cut(s) 166, 217, 843
VpaK11BI GGWCC 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 279
XbaI TCTAGA 1 cut(s) 598
XceI RCATGY 1 cut(s) 477
XcmI CCANNNNNNNNNTGG 2 cut(s) 529, 571
XmnI GAANNNNTTC 2 cut(s) 460, 771
XspI CTAG 3 cut(s) 182, 200, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.