Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004215

Physical Location & Seq

Genomic Coordinates Reverse (-)

30564975 .. 30567419

Gene Structure 2445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G021920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGCATAGGATTATGGCTTCCATTGGCATTGCTATCCTTCTCTTCTCACTATATACTCCATGTAAAGGAGCTCAACAAAACAACAATTCTAGCCACATACGCCTGGGATCTTCAATCTTTCCAGAAACTCGCAACAGCTCATGGCCTTCTGCCTCTGGCCAGTTCGAATTCGGCTTTTATCAGCAAGGAAGTGGTTTCGCTGTTGGAATTTGGTTGGTGGGAGTAAACGGAAACACAACTGTAAACACAACTGTATGGACAGCAAACCGTGATGATCCCCCACTGCTGCCCTCACCAAATAATACAGTGCAGCTGCAGTTAGCCAATGATGGCAAGCTTGTTCTAACCAGTAACCAAGGTCATAAAAAAGTGACCAAGGTCATCGCAAGTGCAACAAACGGTTCTGTTTCCTCTGCCTCCATGCTTGATTCTGGAAACTTCGTTCTTTTCGATGAAAACAATAAAGTGATCTGGGAGAGTTTCAGTCATCCTACTGATACTATCCTGGGTGGTCAGACTCTGCCGGCTGGGGGTCAATTAGTCTCCAGTTTGTCCGATGATGACCACTCAACAGGACATTTTCATCTCAACATGCAGCTTGACGGAAACCTTGTTCTGTACCCAACAAACTCTGAAAACTCTCCGGTAGATGCCTACTATGCTTCAGGTACTAATGGGATCTTGAACCTTCACCTCTATCTTAACAGTACAGATGGCCGTCTGGCTCTAATCAACACCACCAGCTCGGAAGTTTACAGTGACATCAATTTGGGTGGTGGTAATGATGATCTAGCCAATAACACCGTATATCGGGCAACTGTTAGCGTGAACGGAGTTCTACGGGTGTATTCTCATCAGATGGATCCTGACAAAGGCAAACTCCAAGCACCTGTTATCATGCGGTACACACCAGATGATCCTTGCGATGTTAATGGCGTGTGTGGCCTTAACAGCTATTGCACACTCAATGATGCCCAACCAGATTGTCATTGCCTTCCTGGATCAGATTACGCTGACCCCAACAGCATGACTATGGGTTGCTTGAGAAACTATTCAGAACCGGCATGTGCAGCTGGCGGGAAAGAGAATACAACCTTTTATGACATAGTCACCATGGAAAATATGGCCTGGGTAGATACTCCTGACTTTGAAGCCACAATGACCATAGAAGAATGCAAGAGGTCTTGTCTAGAGGACTGCAATTGTGGAGCAGCTCTGTTCGAGCAGACGAACTGTATGAAACATAAGCCACCACTCAGATATTGGAGAAGAGATCTACAGAAATCCAACACAGCTTTCTTCAAGGTAAGCAACTTAACAAGTCCTGAAAGCAAGAATAATGGAACTGAAACTAAAGAACCCACTACGGTTATTATCCGAAGCAAGAAAGTCATTGTGCAAATTCTTGTTTTAACCTTGGGTCTCATCCTCTTCTCATGTGTTGCGCTTGCAATATCTGGATTTTACATTTTCAAAATTCGAGTTCTGAGGTACAAAAGGCTGACAGAGGTTAATGGGAATCTGGGGCTGGCTGATGAGGAACTCACTATGAGAGTTTTTTCATACAACGAGCTGAAGAGGGCTACAAATGGTTTCAAGCAAGAATTGGGGAAGGGCTCCTTCGGAGCAGTTTACAAAGGGGCTCTAAATAGAGGGAAAAAACTCATTGCAGTGAAAAGACTAGAGAAGTTGGTGGAAGAAGGTGAGAGGGAGTTTCGAGCGGAGATGCAAGCAATTGGAAGAACTCATCACAAGAACTTAGTTCGGTTGCTGGGTTACTCTGTTGAGGACTCGAAAAGGCTCCTGGTCTATGAATATATGAGCAATGGCTCCCTTGCAGATCTTCTTTTCAAGAAAGAATGGCATCCAGCTTGGGATGAAAGAGTGAGAATTGCAGTTGATGTCGCAAGAGGTCTCCTCTTTTTACATGAAGAGTGCAAGTCCCCTGTCATTCATTGCGACATAAAGCCTCAAAACATTCTGATGGATGAATTCTGGAATGCAAAAATCTCTGATTTTGGGCTTGCAAAACTGCTAATGCCTGATCAAACAAGGACATTCACAGGGATAAGAGGGACAAGAGGGTATTTGGCACCAGAATGGCAAAAGAACAATCCAATATCAGTGAAGGCAGATATCTACAGTTTTGGTATAGTGCTTCTTGAACTTGTGTGTTGCAGGAGGAACTTGGACGTCAGTGTTAGAGCAGAGGAGATAGTTCTTTCTACTTGGATTTATAAGTCCTTTGTCAGTAGAGAGCTACATAAGCTTATTGTGGGTGGCGAAGAAGTGAATAAGAAGAGCTTGGAGAATATGGTTAAGGTGGGATTATGGTGTATACAAGATGAACCAGCTCTACGTCCATCAATGAAGTCTGTAGTATTGATGTTACAAGGCATTACTGATATATCTATCCCTCCATGTCCAACCACTGCTTCTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin Pkinase S_locus_glycop

Protein Analysis

814

Amino Acids

90.25

Weight (kDa)

6.41

Isoelectric Point (pI)

35.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	83 - 174	1e-17	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	532 - 795	2.6e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	533 - 794	2.4e-47	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004215)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23400
prunus_persica	Prupe.6G165300_v2.0.a1 Prupe.6G165500_v2.0.a1 Prupe.6G165500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118691 RchiOBHm_Chr2g0118751 RchiOBHm_Chr2g0118771
rosa_laevigata	RLG00000018420
rosa_multiflora	Rmu_co8322425.1_g000001 Rmu_sc0001814.1_g000023 Rmu_sc0001814.1_g000024 Rmu_sc0001814.1_g000031 Rmu_sc0002831.1_g000032 Rmu_sc0003564.1_g000001 Rmu_sc0004345.1_g000011
rosa_roxburghii	Rroxscaffold_2G00124740 Rroxscaffold_2G00124750 Rroxscaffold_2G00124840 Rroxscaffold_2G00124870 Rroxscaffold_2G00124880 Rroxscaffold_2G00125530
rosa_rugosa	Rorug02G0218500
rosa_samantha	Rh2AG274800 Rh2AG275100 Rh2AG275400 Rh2BG286900 Rh2BG287300 Rh2DG282600 Rh2DG301600 Rh2DG301800
rosa_wichuraiana	Rw2G021860 Rw2G021880 Rw2G021920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2238
AatII	GACGTC	1 cut(s)	2196
AccB1I	GGYRCC	1 cut(s)	2092
AccBSI	CCGCTC	1 cut(s)	1721
AccI	GTMKAC	1 cut(s)	2338
AciI	CCGC	3 cut(s)	901, 1077, 1721
AclWI	GGATC	7 cut(s)	115, 269, 686, 857, 870, 911, 1009
AcoI	YGGCCR	2 cut(s)	157, 715
AcsI	RAATTY	5 cut(s)	167, 207, 1401, 1476, 1991
AcuI	CTGAAG	2 cut(s)	648, 1595
AcyI	GRCGYC	1 cut(s)	2193
AfaI	GTAC	5 cut(s)	620, 670, 709, 905, 1493
AfiI	CCNNNNNNNGG	4 cut(s)	65, 530, 810, 872
AgsI	TTSAA	8 cut(s)	114, 685, 1151, 1303, 1474, 1597, 1852, 2165
AjnI	CCWGG	5 cut(s)	102, 504, 997, 1127, 1803
AjuI	GAANNNNNNNTTGG	3 cut(s)	1280, 1312, 2419
AloI	GAACNNNNNNTCC	4 cut(s)	426, 458, 597, 629
Alw21I	GWGCWC	1 cut(s)	73
Alw26I	GTCTC	3 cut(s)	547, 1427, 1919
AlwI	GGATC	7 cut(s)	115, 269, 686, 857, 870, 911, 1009
AlwNI	CAGNNNCTG	1 cut(s)	520
AoxI	GGCC	5 cut(s)	143, 157, 715, 943, 1125
ApeKI	GCWGC	6 cut(s)	286, 310, 313, 595, 1070, 1211
ApoI	RAATTY	5 cut(s)	167, 207, 1401, 1476, 1991
Asp700I	GAANNNNTTC	1 cut(s)	1051
AspLEI	GCGC	1 cut(s)	1447
AsuHPI	GGTGA	4 cut(s)	285, 683, 1102, 1715
AsuII	TTCGAA	1 cut(s)	165
BalI	TGGCCA	1 cut(s)	159
BamHI	GGATCC	1 cut(s)	862
BanI	GGYRCC	1 cut(s)	2092
BanII	GRGCYC	3 cut(s)	73, 1619, 1645
Bbv12I	GWGCWC	1 cut(s)	73
BbvI	GCAGC	6 cut(s)	273, 300, 322, 607, 1082, 1223
BccI	CCATC	5 cut(s)	323, 707, 853, 1978, 2371
BceAI	ACGGC	1 cut(s)	702
BciT130I	CCWGG	5 cut(s)	104, 506, 999, 1129, 1805
BclI	TGATCA	1 cut(s)	2044
BcoDI	GTCTC	3 cut(s)	547, 1427, 1919
BfaI	CTAG	4 cut(s)	90, 791, 1190, 1682
BfmI	CTRYAG	4 cut(s)	314, 1277, 2140, 2376
BglII	AGATCT	2 cut(s)	1273, 1840
BisI	GCNGC	6 cut(s)	287, 311, 314, 596, 1071, 1212
BlsI	GCNGC	6 cut(s)	288, 312, 315, 597, 1072, 1213
Bme1390I	CCNGG	5 cut(s)	104, 506, 999, 1129, 1805
BmiI	GGNNCC	5 cut(s)	864, 1618, 1802, 1831, 2094
BmrFI	CCNGG	5 cut(s)	104, 506, 999, 1129, 1805
BmsI	GCATC	4 cut(s)	640, 961, 1716, 1873
BoxI	GACNNNNGTC	1 cut(s)	377
BplI	GAGNNNNNCTC	2 cut(s)	1902, 1934
BpmI	CTGGAG	1 cut(s)	529
Bpu14I	TTCGAA	1 cut(s)	165
BpuEI	CTTGAG	1 cut(s)	1063
BsaHI	GRCGYC	1 cut(s)	2193
BsaI	GGTCTC	2 cut(s)	1427, 1919
BsaJI	CCNNGG	7 cut(s)	103, 355, 375, 505, 1113, 1128, 1416
BsaWI	WCCGGW	1 cut(s)	643
Bsc4I	CCNNNNNNNGG	4 cut(s)	65, 530, 810, 872
Bse118I	RCCGGY	2 cut(s)	523, 1060
Bse1I	ACTGG	3 cut(s)	160, 348, 546
Bse3DI	GCAATG	5 cut(s)	27, 988, 1665, 1831, 1954
BseBI	CCWGG	5 cut(s)	104, 506, 999, 1129, 1805
BseDI	CCNNGG	7 cut(s)	103, 355, 375, 505, 1113, 1128, 1416
BseGI	GGATG	5 cut(s)	487, 1425, 1864, 1882, 1993
BseLI	CCNNNNNNNGG	4 cut(s)	65, 530, 810, 872
BseMI	GCAATG	5 cut(s)	27, 988, 1665, 1831, 1954
BseMII	CTCAG	2 cut(s)	1270, 1478
BseNI	ACTGG	3 cut(s)	160, 348, 546
BseRI	GAGGAG	2 cut(s)	1907, 2225
BseXI	GCAGC	6 cut(s)	273, 300, 322, 607, 1082, 1223
BseYI	CCCAGC	2 cut(s)	527, 1771
BsgI	GTGCAG	2 cut(s)	329, 1089
BshFI	GGCC	5 cut(s)	145, 159, 717, 945, 1127
BshNI	GGYRCC	1 cut(s)	2092
BsiHKAI	GWGCWC	1 cut(s)	73
BsiSI	CCGG	3 cut(s)	524, 644, 1061
BslFI	GGGAC	2 cut(s)	1927, 2089
BslI	CCNNNNNNNGG	4 cut(s)	65, 530, 810, 872
BsmAI	GTCTC	3 cut(s)	547, 1427, 1919
BsmFI	GGGAC	2 cut(s)	1927, 2089
BsmI	GAATGC	2 cut(s)	1178, 2005
BsnI	GGCC	5 cut(s)	145, 159, 717, 945, 1127
Bso31I	GGTCTC	2 cut(s)	1427, 1919
Bsp119I	TTCGAA	1 cut(s)	165
Bsp1286I	GDGCHC	3 cut(s)	73, 1619, 1645
Bsp19I	CCATGG	1 cut(s)	1113
BspACI	CCGC	3 cut(s)	901, 1077, 1721
BspANI	GGCC	5 cut(s)	145, 159, 717, 945, 1127
BspCNI	CTCAG	2 cut(s)	1269, 1479
BspLI	GGNNCC	5 cut(s)	864, 1618, 1802, 1831, 2094
BspMAI	CTGCAG	1 cut(s)	318
BspPI	GGATC	7 cut(s)	115, 269, 686, 857, 870, 911, 1009
BspQI	GCTCTTC	1 cut(s)	2294
BspT104I	TTCGAA	1 cut(s)	165
BspT107I	GGYRCC	1 cut(s)	2092
BspTNI	GGTCTC	2 cut(s)	1427, 1919
BsrBI	CCGCTC	1 cut(s)	1721
BsrDI	GCAATG	5 cut(s)	27, 988, 1665, 1831, 1954
BsrFI	RCCGGY	2 cut(s)	523, 1060
BsrI	ACTGG	3 cut(s)	160, 348, 546
BssAI	RCCGGY	2 cut(s)	523, 1060
BssECI	CCNNGG	7 cut(s)	103, 355, 375, 505, 1113, 1128, 1416
BssNAI	GTATAC	1 cut(s)	2339
BssNI	GRCGYC	1 cut(s)	2193
BssT1I	CCWWGG	4 cut(s)	355, 375, 1113, 1416
Bst1107I	GTATAC	1 cut(s)	2339
Bst2UI	CCWGG	5 cut(s)	104, 506, 999, 1129, 1805
Bst6I	CTCTTC	6 cut(s)	47, 1264, 1436, 1571, 1926, 2294
BstACI	GRCGYC	1 cut(s)	2193
BstBI	TTCGAA	1 cut(s)	165
BstC8I	GCNNGC	7 cut(s)	335, 525, 1075, 1449, 1530, 1731, 2025
BstDEI	CTNAG	3 cut(s)	1256, 1487, 1759
BstDSI	CCRYGG	1 cut(s)	1113
BstENI	CCTNNNNNAGG	1 cut(s)	870
BstF5I	GGATG	5 cut(s)	487, 1425, 1864, 1882, 1993
BstHHI	GCGC	1 cut(s)	1447
BstMAI	GTCTC	3 cut(s)	547, 1427, 1919
BstMWI	GCNNNNNNNGC	6 cut(s)	99, 659, 942, 951, 1070, 2266
BstNI	CCWGG	5 cut(s)	104, 506, 999, 1129, 1805
BstNSI	RCATGY	2 cut(s)	595, 1068
BstPAI	GACNNNNGTC	1 cut(s)	377
BstSCI	CCNGG	5 cut(s)	102, 504, 997, 1127, 1803
BstSFI	CTRYAG	4 cut(s)	314, 1277, 2140, 2376
BstV1I	GCAGC	6 cut(s)	273, 300, 322, 607, 1082, 1223
BstX2I	RGATCY	5 cut(s)	107, 678, 862, 1273, 1840
BstYI	RGATCY	5 cut(s)	107, 678, 862, 1273, 1840
BstZ17I	GTATAC	1 cut(s)	2339
BsuRI	GGCC	5 cut(s)	145, 159, 717, 945, 1127
BtgI	CCRYGG	1 cut(s)	1113
BtgZI	GCGATG	2 cut(s)	367, 939
BtsCI	GGATG	5 cut(s)	487, 1425, 1864, 1882, 1993
BtsI	GCAGTG	3 cut(s)	281, 1677, 2430
BtsIMutI	CAGTG	7 cut(s)	281, 312, 763, 1677, 2130, 2203, 2430
Cac8I	GCNNGC	7 cut(s)	335, 525, 1075, 1449, 1530, 1731, 2025
CaiI	CAGNNNCTG	1 cut(s)	520
CfoI	GCGC	1 cut(s)	1447
Cfr10I	RCCGGY	2 cut(s)	523, 1060
Csp6I	GTAC	5 cut(s)	619, 669, 708, 904, 1492
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CviQI	GTAC	5 cut(s)	619, 669, 708, 904, 1492
DdeI	CTNAG	3 cut(s)	1256, 1487, 1759
EaeI	YGGCCR	2 cut(s)	157, 715
Eam1104I	CTCTTC	6 cut(s)	47, 1264, 1436, 1571, 1926, 2294
EarI	CTCTTC	6 cut(s)	47, 1264, 1436, 1571, 1926, 2294
Ecl136II	GAGCTC	1 cut(s)	71
Eco130I	CCWWGG	4 cut(s)	355, 375, 1113, 1416
Eco24I	GRGCYC	3 cut(s)	73, 1619, 1645
Eco31I	GGTCTC	2 cut(s)	1427, 1919
Eco32I	GATATC	1 cut(s)	2137
Eco53kI	GAGCTC	1 cut(s)	71
Eco57I	CTGAAG	2 cut(s)	648, 1595
EcoICRI	GAGCTC	1 cut(s)	71
EcoNI	CCTNNNNNAGG	1 cut(s)	870
EcoRI	GAATTC	2 cut(s)	167, 1991
EcoRII	CCWGG	5 cut(s)	102, 504, 997, 1127, 1803
EcoRV	GATATC	1 cut(s)	2137
EcoT14I	CCWWGG	4 cut(s)	355, 375, 1113, 1416
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	73, 1619, 1645
ErhI	CCWWGG	4 cut(s)	355, 375, 1113, 1416
FalI	AAGNNNNNCTT	4 cut(s)	1604, 1636, 2288, 2320
FaqI	GGGAC	2 cut(s)	1927, 2089
FauI	CCCGC	1 cut(s)	1070
FbaI	TGATCA	1 cut(s)	2044
FblI	GTMKAC	1 cut(s)	2338
Fnu4HI	GCNGC	6 cut(s)	287, 311, 314, 596, 1071, 1212
FokI	GGATG	5 cut(s)	474, 1412, 1851, 1889, 2000
FriOI	GRGCYC	3 cut(s)	73, 1619, 1645
Fsp4HI	GCNGC	6 cut(s)	287, 311, 314, 596, 1071, 1212
FspBI	CTAG	4 cut(s)	90, 791, 1190, 1682
GlaI	GCGC	1 cut(s)	1446
GluI	GCNGC	6 cut(s)	287, 311, 314, 596, 1071, 1212
GsaI	CCCAGC	2 cut(s)	531, 1775
GsuI	CTGGAG	1 cut(s)	529
HaeIII	GGCC	5 cut(s)	145, 159, 717, 945, 1127
HapII	CCGG	3 cut(s)	524, 644, 1061
HhaI	GCGC	1 cut(s)	1447
Hin1I	GRCGYC	1 cut(s)	2193
Hin6I	GCGC	1 cut(s)	1445
HinP1I	GCGC	1 cut(s)	1445
HindIII	AAGCTT	2 cut(s)	335, 2267
HinfI	GANTC	4 cut(s)	428, 517, 1519, 1790
HpaII	CCGG	3 cut(s)	524, 644, 1061
HphI	GGTGA	4 cut(s)	285, 683, 1102, 1715
Hpy166II	GTNNAC	7 cut(s)	226, 244, 754, 829, 906, 1633, 2339
Hpy8I	GTNNAC	7 cut(s)	226, 244, 754, 829, 906, 1633, 2339
HpyAV	CCTTC	8 cut(s)	47, 156, 698, 1004, 1606, 1630, 1694, 2122
HpyCH4IV	ACGT	2 cut(s)	2193, 2359
HpyF10VI	GCNNNNNNNGC	6 cut(s)	99, 659, 942, 951, 1070, 2266
HpyF3I	CTNAG	3 cut(s)	1256, 1487, 1759
HpySE526I	ACGT	2 cut(s)	2193, 2359
Hsp92I	GRCGYC	1 cut(s)	2193
HspAI	GCGC	1 cut(s)	1445
KroI	GCCGGC	1 cut(s)	523
KroNI	GCCGGC	1 cut(s)	525
Ksp22I	TGATCA	1 cut(s)	2044
LguI	GCTCTTC	1 cut(s)	2294
LmnI	GCTCC	6 cut(s)	68, 1208, 1622, 1625, 1806, 1835
Lsp1109I	GCAGC	6 cut(s)	273, 300, 322, 607, 1082, 1223
LweI	GCATC	4 cut(s)	640, 961, 1716, 1873
MaeI	CTAG	4 cut(s)	90, 791, 1190, 1682
MaeII	ACGT	2 cut(s)	2193, 2359
MaeIII	GTNAC	6 cut(s)	350, 370, 758, 1108, 1775, 2388
MbiI	CCGCTC	1 cut(s)	1721
MfeI	CAATTG	2 cut(s)	1201, 1734
MflI	RGATCY	5 cut(s)	107, 678, 862, 1273, 1840
MhlI	GDGCHC	3 cut(s)	73, 1619, 1645
MlsI	TGGCCA	1 cut(s)	159
MluNI	TGGCCA	1 cut(s)	159
MlyI	GAGTC	2 cut(s)	511, 1784
MmeI	TCCRAC	2 cut(s)	184, 1311
Mox20I	TGGCCA	1 cut(s)	159
Mph1103I	ATGCAT	1 cut(s)	6
MroNI	GCCGGC	1 cut(s)	523
MroXI	GAANNNNTTC	1 cut(s)	1051
MscI	TGGCCA	1 cut(s)	159
MseI	TTAA	7 cut(s)	702, 930, 948, 1316, 1412, 1512, 2319
MslI	CAYNNNNRTG	4 cut(s)	1031, 1670, 1982, 2098
Msp20I	TGGCCA	1 cut(s)	159
MspA1I	CMGCKG	2 cut(s)	313, 1073
MspI	CCGG	3 cut(s)	524, 644, 1061
MspR9I	CCNGG	5 cut(s)	104, 506, 999, 1129, 1805
MunI	CAATTG	2 cut(s)	1201, 1734
Mva1269I	GAATGC	2 cut(s)	1178, 2005
MvaI	CCWGG	5 cut(s)	104, 506, 999, 1129, 1805
MwoI	GCNNNNNNNGC	6 cut(s)	99, 659, 942, 951, 1070, 2266
NaeI	GCCGGC	1 cut(s)	525
NcoI	CCATGG	1 cut(s)	1113
NgoMIV	GCCGGC	1 cut(s)	523
NlaIV	GGNNCC	5 cut(s)	864, 1618, 1802, 1831, 2094
NmuCI	GTSAC	3 cut(s)	370, 758, 1108
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	595, 1068
NspV	TTCGAA	1 cut(s)	165
PciSI	GCTCTTC	1 cut(s)	2294
PcsI	WCGNNNNNNNCGW	1 cut(s)	447
PctI	GAATGC	2 cut(s)	1178, 2005
PdiI	GCCGGC	1 cut(s)	525
PdmI	GAANNNNTTC	1 cut(s)	1051
PfeI	GAWTC	2 cut(s)	428, 1519
PflFI	GACNNNGTC	1 cut(s)	1106
PfoI	TCCNGGA	1 cut(s)	997
PkrI	GCNGC	6 cut(s)	288, 312, 315, 597, 1072, 1213
PleI	GAGTC	2 cut(s)	511, 1784
PpsI	GAGTC	2 cut(s)	511, 1784
PshAI	GACNNNNGTC	1 cut(s)	377
PsiI	TTATAA	1 cut(s)	2238
Psp124BI	GAGCTC	1 cut(s)	73
Psp6I	CCWGG	5 cut(s)	102, 504, 997, 1127, 1803
PspFI	CCCAGC	2 cut(s)	527, 1771
PspGI	CCWGG	5 cut(s)	102, 504, 997, 1127, 1803
PspN4I	GGNNCC	5 cut(s)	864, 1618, 1802, 1831, 2094
PsrI	GAACNNNNNNTAC	2 cut(s)	2340, 2372
PstI	CTGCAG	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	520
PsuI	RGATCY	5 cut(s)	107, 678, 862, 1273, 1840
PsyI	GACNNNGTC	1 cut(s)	1106
PvuII	CAGCTG	2 cut(s)	313, 1073
RsaI	GTAC	5 cut(s)	620, 670, 709, 905, 1493
RsaNI	GTAC	5 cut(s)	619, 669, 708, 904, 1492
RseI	CAYNNNNRTG	4 cut(s)	1031, 1670, 1982, 2098
SacI	GAGCTC	1 cut(s)	73
SapI	GCTCTTC	1 cut(s)	2294
SaqAI	TTAA	7 cut(s)	702, 930, 948, 1316, 1412, 1512, 2319
SatI	GCNGC	6 cut(s)	287, 311, 314, 596, 1071, 1212
SchI	GAGTC	2 cut(s)	511, 1784
ScrFI	CCNGG	5 cut(s)	104, 506, 999, 1129, 1805
SduI	GDGCHC	3 cut(s)	73, 1619, 1645
SfaNI	GCATC	4 cut(s)	640, 961, 1716, 1873
SfcI	CTRYAG	4 cut(s)	314, 1277, 2140, 2376
SfuI	TTCGAA	1 cut(s)	165
SmiMI	CAYNNNNRTG	4 cut(s)	1031, 1670, 1982, 2098
SmlI	CTYRAG	1 cut(s)	1042
SmoI	CTYRAG	1 cut(s)	1042
SsiI	CCGC	3 cut(s)	901, 1077, 1721
SspMI	CTAG	4 cut(s)	90, 791, 1190, 1682
SstI	GAGCTC	1 cut(s)	73
StyD4I	CCNGG	5 cut(s)	102, 504, 997, 1127, 1803
StyI	CCWWGG	4 cut(s)	355, 375, 1113, 1416
TaiI	ACGT	2 cut(s)	2196, 2362
TaqI	TCGA	6 cut(s)	165, 450, 1221, 1480, 1717, 1793
TatI	WGTACW	1 cut(s)	707
TfiI	GAWTC	2 cut(s)	428, 1519
Tru1I	TTAA	7 cut(s)	702, 930, 948, 1316, 1412, 1512, 2319
Tru9I	TTAA	7 cut(s)	702, 930, 948, 1316, 1412, 1512, 2319
TscAI	CASTG	7 cut(s)	288, 312, 763, 1677, 2130, 2203, 2437
TseFI	GTSAC	3 cut(s)	370, 758, 1108
TseI	GCWGC	6 cut(s)	286, 310, 313, 595, 1070, 1211
Tsp45I	GTSAC	3 cut(s)	370, 758, 1108
TspGWI	ACGGA	3 cut(s)	243, 618, 846
TspRI	CASTG	7 cut(s)	288, 312, 763, 1677, 2130, 2203, 2437
Tth111I	GACNNNGTC	1 cut(s)	1106
XagI	CCTNNNNNAGG	1 cut(s)	870
XapI	RAATTY	5 cut(s)	167, 207, 1401, 1476, 1991
XbaI	TCTAGA	1 cut(s)	1189
XceI	RCATGY	2 cut(s)	595, 1068
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1120, 1260
XmiI	GTMKAC	1 cut(s)	2338
XmnI	GAANNNNTTC	1 cut(s)	1051
XspI	CTAG	4 cut(s)	90, 791, 1190, 1682
ZraI	GACGTC	1 cut(s)	2194
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.