Rh2BG394500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008183
Physical Location & Seq
Forward (+)
55938342 .. 55941452
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG394500.1

Sequence Viewer

Length: 1716 bp
ATGTCTATTAAGAAAGTGGTGACAATTTGCGAGATTGGATTTGGGTCAATTCAGATAATAGGGTCTCCCACATTGCACCTGAAAGTCTGCCAACTAATGGTGGAGGGGTTAGATCCGTTGAATGAACTTGTTATCATACATGGAAAGGAGCATGTCATATCGGCAAAAGACTTTGCTCGGATAATGGGAATCAACGATGATGGAAGTGATATAGAGTTGAGTGGGAAGAGTGATGAAGATCTTGTAAACAGGTTGAAACTATGTTTAGCAGGGGGAGGAGATGCTATCACAATAGCAACATTGAGGGAGATTGTGGTTAAAAGAAAGGAAGCTGACAATTTGTTCGTGATGGGTTTCATCCTTTATGCCATGGAAACGCTGTTGTGTCCAGTACCGACGAAGGTATCGAGGATAACTATACATGGAGCGAATGCTGCTAAAAGAGCACGGACCGATAAGTGGGAAAGAGGTGGTGAGGCGATGGCAATTGAGGAGGAAGTTGAACAGCTGAAAATGGGTTTTGTTAAGCTGGAGGAAGCTGTTGTAGTTATAATGCCAGAGGAAGCTGTTGTAGTTATAATGCCAGAGATAGCTACCATGAAGGAAACCGTTGACAGGATGAGTAGAAACATATCGAGCTCACAGCAACGTGATAAAGATGTAGAATCAACACACTTCAATCATGGAAGGGAACGCGAGGAAGGTTTGGGGAGTTACTCATTGAAGTTCTTGTATGTCAACAAAGGCGAACGTCGGGATGGGACAAGAAGGCAGCCACATGATCTATGTACGGAAGGTAAGGAAGAGCCGCAGGTTTCAGAGCCATTAGCAGTATCAGTAAGCCCGACAGGGCCTCCCAAGGCAGGGCATAGAAGGATGAAAGATAAAACTTGGAGGCTGAAAACAGTAATATCGAAGAGATATGTTCGGAAATTATTCCCCAAGTCACCATCAAGGGTTGCAGCAAATTTCCTCAATGGAAGGCAGTTGGTAACCATAAACAGGAAAAAGGAAGGTGTTTTGGCAGGGTCGTTCATGGTAAACCGGGATACATCTGACACGGTCGTAGAAATGTTGAAGTTTGTGTTTATGCAGCGGCCACTAACTGCAAAGATGGCACGCGTAAATCGGGACGACCTTGAAGCTTCTCTTGCACCAGATGGACTAGTTTGTAGCCATGTTATAAACATGTTTGCTACATACCTAAATGAGCCAAAAGGCCATGTTTGGTACTTTAACACATACTTTGGGGGTGGTGCAACCAATTTCGATGTCTCAATTAGGTACATTGGATGGGAGGAGCGTATTAGAAGCGTATGCAGACTAAATGATTTCGCTCCTACATTGAAGGAATGCACAAAGTTGGTCATGTTGGACGTGGTTTTTAAGAATGAAATTGTAGGAGCATGTATTCAGCGTGCACTGGTGTTCAAAGACTTTACAAAGGTGGAACCCATTGCAAGTCCCAGGCAGGAGGGAGTTGTGAACTGTGGGGTTTTTCTAATCCGGAATATGCAGAACTATGGTCGTGATTGGGCAAAGAAGTATGTGTTGGAAGACCACAGAGCTTCCCTTGTTATGGAATGTATAGGGAACAACCGAAATGAGGTCGAGAATTTGGAGGAAATGATGGTGGAATGCTTGCACAATCTTGAGCAACTACCAATTCTGCAGTTAGACGAAATGAATGAAGGAGGAGTGGGGAAACAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

571

Amino Acids

64.29

Weight (kDa)

7.17

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 551, 578, 1184
Acc36I ACCTGC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 97
AccII CGCG 2 cut(s) 696, 1122
AccIII TCCGGA 1 cut(s) 1506
AciI CCGC 2 cut(s) 809, 1096
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 1097
AcsI RAATTY 2 cut(s) 967, 1615
AfaI GTAC 4 cut(s) 393, 790, 1232, 1286
AfiI CCNNNNNNNGG 8 cut(s) 97, 459, 615, 863, 864, 1002, 1579, 1606
AflIII ACRYGT 2 cut(s) 1120, 1188
AgsI TTSAA 9 cut(s) 121, 256, 503, 679, 724, 1078, 1142, 1348, 1432
AhlI ACTAGT 1 cut(s) 1165
AjiI CACGTC 1 cut(s) 1378
AjnI CCWGG 1 cut(s) 1466
AjuI GAANNNNNNNTTGG 2 cut(s) 1535, 1567
AluBI AGCT 9 cut(s) 332, 508, 529, 539, 566, 593, 639, 1145, 1568
AluI AGCT 9 cut(s) 332, 508, 529, 539, 566, 593, 639, 1145, 1568
Alw21I GWGCWC 3 cut(s) 448, 641, 1423
Alw26I GTCTC 2 cut(s) 69, 1279
Alw44I GTGCAC 1 cut(s) 1419
AlwI GGATC 1 cut(s) 107
Aor13HI TCCGGA 1 cut(s) 1506
AoxI GGCC 3 cut(s) 851, 1097, 1219
ApaLI GTGCAC 1 cut(s) 1419
ApeKI GCWGC 4 cut(s) 434, 772, 962, 1093
ApoI RAATTY 2 cut(s) 967, 1615
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 935
AspS9I GGNCC 2 cut(s) 450, 851
AsuC2I CCSGG 1 cut(s) 1046
AsuHPI GGTGA 3 cut(s) 31, 485, 939
AvaII GGWCC 1 cut(s) 450
BaeGI GKGCMC 1 cut(s) 1423
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BanII GRGCYC 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 1563
Bbv12I GWGCWC 3 cut(s) 448, 641, 1423
BbvI GCAGC 4 cut(s) 421, 784, 974, 1105
BccI CCATC 9 cut(s) 194, 343, 475, 752, 958, 1108, 1154, 1287, 1624
BciT130I CCWGG 1 cut(s) 1468
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 1046
BcoDI GTCTC 2 cut(s) 69, 1279
BcuI ACTAGT 1 cut(s) 1165
BfaI CTAG 1 cut(s) 1166
BfmI CTRYAG 1 cut(s) 1670
BfuAI ACCTGC 1 cut(s) 802
BfuI GTATCC 1 cut(s) 1042
BglII AGATCT 1 cut(s) 238
BisI GCNGC 6 cut(s) 435, 773, 809, 963, 1094, 1097
BlsI GCNGC 6 cut(s) 436, 774, 810, 964, 1095, 1098
Bme1390I CCNGG 2 cut(s) 1046, 1468
Bme18I GGWCC 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 1378
BmgT120I GGNCC 2 cut(s) 450, 851
BmiI GGNNCC 1 cut(s) 1452
BmrFI CCNGG 2 cut(s) 1046, 1468
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 1563
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 1 cut(s) 1046
BsaBI GATNNNNATC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 369, 858, 1466
BsaWI WCCGGW 1 cut(s) 1506
BsaXI ACNNNNNCTCC 2 cut(s) 838, 868
Bsc4I CCNNNNNNNGG 8 cut(s) 97, 459, 615, 863, 864, 1002, 1579, 1606
Bse1I ACTGG 2 cut(s) 389, 1428
Bse3DI GCAATG 2 cut(s) 71, 1455
Bse8I GATNNNNATC 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 1506
BseBI CCWGG 1 cut(s) 1468
BseDI CCNNGG 3 cut(s) 369, 858, 1466
BseGI GGATG 5 cut(s) 357, 624, 763, 882, 1298
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 8 cut(s) 97, 459, 615, 863, 864, 1002, 1579, 1606
BseMI GCAATG 2 cut(s) 71, 1455
BseNI ACTGG 2 cut(s) 389, 1428
BseRI GAGGAG 4 cut(s) 291, 506, 1313, 1710
BseSI GKGCMC 1 cut(s) 1423
BseXI GCAGC 4 cut(s) 421, 784, 974, 1105
Bsh1236I CGCG 2 cut(s) 696, 1122
Bsh1285I CGRYCG 1 cut(s) 1065
BshFI GGCC 3 cut(s) 853, 1099, 1221
BsiEI CGRYCG 1 cut(s) 1065
BsiHKAI GWGCWC 3 cut(s) 448, 641, 1423
BsiSI CCGG 2 cut(s) 1045, 1507
BslFI GGGAC 3 cut(s) 775, 1145, 1449
BslI CCNNNNNNNGG 8 cut(s) 97, 459, 615, 863, 864, 1002, 1579, 1606
BsmAI GTCTC 2 cut(s) 69, 1279
BsmFI GGGAC 3 cut(s) 775, 1145, 1449
BsmI GAATGC 3 cut(s) 436, 1358, 1643
BsnI GGCC 3 cut(s) 853, 1099, 1221
Bso31I GGTCTC 1 cut(s) 69
Bsp1286I GDGCHC 3 cut(s) 448, 641, 1423
Bsp13I TCCGGA 1 cut(s) 1506
Bsp143I GATC 3 cut(s) 112, 238, 781
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 2 cut(s) 809, 1096
BspANI GGCC 3 cut(s) 853, 1099, 1221
BspEI TCCGGA 1 cut(s) 1506
BspFNI CGCG 2 cut(s) 696, 1122
BspLI GGNNCC 1 cut(s) 1452
BspMAI CTGCAG 1 cut(s) 1674
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 1 cut(s) 107
BspQI GCTCTTC 1 cut(s) 798
BspTNI GGTCTC 1 cut(s) 69
BsrDI GCAATG 2 cut(s) 71, 1455
BsrI ACTGG 2 cut(s) 389, 1428
BssECI CCNNGG 3 cut(s) 369, 858, 1466
BssMI GATC 3 cut(s) 112, 238, 781
BssT1I CCWWGG 2 cut(s) 369, 858
Bst2UI CCWGG 1 cut(s) 1468
Bst4CI ACNGT 4 cut(s) 610, 907, 1063, 1490
Bst6I CTCTTC 3 cut(s) 221, 798, 911
BstC8I GCNNGC 3 cut(s) 1120, 1419, 1643
BstDSI CCRYGG 1 cut(s) 369
BstEII GGTNACC 1 cut(s) 991
BstF5I GGATG 5 cut(s) 357, 624, 763, 882, 1298
BstFNI CGCG 2 cut(s) 696, 1122
BstKTI GATC 3 cut(s) 115, 241, 784
BstMAI GTCTC 2 cut(s) 69, 1279
BstMBI GATC 3 cut(s) 112, 238, 781
BstMCI CGRYCG 1 cut(s) 1065
BstMWI GCNNNNNNNGC 4 cut(s) 434, 443, 1115, 1151
BstNI CCWGG 1 cut(s) 1468
BstNSI RCATGY 3 cut(s) 155, 1192, 1410
BstPI GGTNACC 1 cut(s) 991
BstSCI CCNGG 2 cut(s) 1044, 1466
BstSFI CTRYAG 1 cut(s) 1670
BstSLI GKGCMC 1 cut(s) 1423
BstUI CGCG 2 cut(s) 696, 1122
BstV1I GCAGC 4 cut(s) 421, 784, 974, 1105
BstV2I GAAGAC 1 cut(s) 1563
BstX2I RGATCY 2 cut(s) 112, 238
BstYI RGATCY 2 cut(s) 112, 238
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 3 cut(s) 853, 1099, 1221
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 494
BtrI CACGTC 1 cut(s) 1378
BtsCI GGATG 5 cut(s) 357, 624, 763, 882, 1298
BtsIMutI CAGTG 1 cut(s) 1421
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 3 cut(s) 1120, 1419, 1643
Cfr13I GGNCC 2 cut(s) 450, 851
CpoI CGGWCCG 1 cut(s) 450
Csp6I GTAC 4 cut(s) 392, 789, 1231, 1285
CspI CGGWCCG 1 cut(s) 450
CviQI GTAC 4 cut(s) 392, 789, 1231, 1285
DpnI GATC 3 cut(s) 114, 240, 783
DpnII GATC 3 cut(s) 112, 238, 781
EaeI YGGCCR 1 cut(s) 1097
Eam1104I CTCTTC 3 cut(s) 221, 798, 911
EarI CTCTTC 3 cut(s) 221, 798, 911
Ecl136II GAGCTC 1 cut(s) 639
Eco130I CCWWGG 2 cut(s) 369, 858
Eco24I GRGCYC 1 cut(s) 641
Eco31I GGTCTC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 991
EcoICRI GAGCTC 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 851
EcoO65I GGTNACC 1 cut(s) 991
EcoRII CCWGG 1 cut(s) 1466
EcoT14I CCWWGG 2 cut(s) 369, 858
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 2 cut(s) 369, 858
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 3 cut(s) 775, 1145, 1449
Fnu4HI GCNGC 6 cut(s) 435, 773, 809, 963, 1094, 1097
FokI GGATG 5 cut(s) 344, 631, 770, 889, 1305
FriOI GRGCYC 1 cut(s) 641
Fsp4HI GCNGC 6 cut(s) 435, 773, 809, 963, 1094, 1097
FspBI CTAG 1 cut(s) 1166
GluI GCNGC 6 cut(s) 435, 773, 809, 963, 1094, 1097
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 3 cut(s) 853, 1099, 1221
HapII CCGG 2 cut(s) 1045, 1507
HincII GTYRAC 2 cut(s) 613, 739
HindII GTYRAC 2 cut(s) 613, 739
HindIII AAGCTT 1 cut(s) 1143
HinfI GANTC 2 cut(s) 189, 665
HpaII CCGG 2 cut(s) 1045, 1507
HphI GGTGA 3 cut(s) 31, 485, 939
Hpy166II GTNNAC 6 cut(s) 247, 613, 739, 1042, 1421, 1486
Hpy188I TCNGA 5 cut(s) 54, 180, 820, 930, 1057
Hpy188III TCNNGA 7 cut(s) 346, 755, 1130, 1507, 1529, 1612, 1652
Hpy8I GTNNAC 6 cut(s) 247, 613, 739, 1042, 1421, 1486
Hpy99I CGWCG 2 cut(s) 400, 756
HpyCH4III ACNGT 4 cut(s) 610, 907, 1063, 1490
HpyCH4IV ACGT 3 cut(s) 649, 751, 1377
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 443, 1115, 1151
HpySE526I ACGT 3 cut(s) 649, 751, 1377
Kpn2I TCCGGA 1 cut(s) 1506
Kzo9I GATC 3 cut(s) 112, 238, 781
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 5 cut(s) 148, 425, 1300, 1342, 1403
Lsp1109I GCAGC 4 cut(s) 421, 784, 974, 1105
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 1166
MaeII ACGT 3 cut(s) 649, 751, 1377
MaeIII GTNAC 4 cut(s) 19, 713, 945, 991
MalI GATC 3 cut(s) 114, 240, 783
MboI GATC 3 cut(s) 112, 238, 781
MboII GAAGA 5 cut(s) 238, 248, 815, 928, 1568
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 2 cut(s) 112, 238
MhlI GDGCHC 3 cut(s) 448, 641, 1423
MluI ACGCGT 1 cut(s) 1120
MmeI TCCRAC 2 cut(s) 1353, 1533
MroI TCCGGA 1 cut(s) 1506
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 5 cut(s) 9, 318, 525, 1236, 1386
MspA1I CMGCKG 2 cut(s) 508, 1096
MspI CCGG 2 cut(s) 1045, 1507
MspR9I CCNGG 2 cut(s) 1046, 1468
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 3 cut(s) 436, 1358, 1643
MvaI CCWGG 1 cut(s) 1468
MvnI CGCG 2 cut(s) 696, 1122
MwoI GCNNNNNNNGC 4 cut(s) 434, 443, 1115, 1151
NciI CCSGG 1 cut(s) 1046
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 112, 238, 781
NlaIV GGNNCC 1 cut(s) 1452
NmuCI GTSAC 2 cut(s) 19, 945
NspI RCATGY 3 cut(s) 155, 1192, 1410
PciI ACATGT 1 cut(s) 1188
PciSI GCTCTTC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 404
PctI GAATGC 3 cut(s) 436, 1358, 1643
PdmI GAANNNNTTC 1 cut(s) 935
PfeI GAWTC 2 cut(s) 189, 665
PflFI GACNNNGTC 1 cut(s) 1061
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 6 cut(s) 436, 774, 810, 964, 1095, 1098
PscI ACATGT 1 cut(s) 1188
PsiI TTATAA 3 cut(s) 551, 578, 1184
Psp124BI GAGCTC 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 1466
PspEI GGTNACC 1 cut(s) 991
PspGI CCWGG 1 cut(s) 1466
PspN4I GGNNCC 1 cut(s) 1452
PspPI GGNCC 2 cut(s) 450, 851
PstI CTGCAG 1 cut(s) 1674
PsuI RGATCY 2 cut(s) 112, 238
PsyI GACNNNGTC 1 cut(s) 1061
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 4 cut(s) 393, 790, 1232, 1286
RsaNI GTAC 4 cut(s) 392, 789, 1231, 1285
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SacI GAGCTC 1 cut(s) 641
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 5 cut(s) 9, 318, 525, 1236, 1386
SatI GCNGC 6 cut(s) 435, 773, 809, 963, 1094, 1097
Sau3AI GATC 3 cut(s) 112, 238, 781
Sau96I GGNCC 2 cut(s) 450, 851
ScrFI CCNGG 2 cut(s) 1046, 1468
SduI GDGCHC 3 cut(s) 448, 641, 1423
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 1670
SinI GGWCC 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 1652
SmoI CTYRAG 1 cut(s) 1652
SpeI ACTAGT 1 cut(s) 1165
SsiI CCGC 2 cut(s) 809, 1096
SspMI CTAG 1 cut(s) 1166
SstI GAGCTC 1 cut(s) 641
StyD4I CCNGG 2 cut(s) 1044, 1466
StyI CCWWGG 2 cut(s) 369, 858
TaaI ACNGT 4 cut(s) 610, 907, 1063, 1490
TaiI ACGT 3 cut(s) 652, 754, 1380
TaqI TCGA 5 cut(s) 407, 635, 914, 1269, 1611
TaqII GACCGA 1 cut(s) 467
TauI GCSGC 2 cut(s) 811, 1099
TfiI GAWTC 2 cut(s) 189, 665
Tru1I TTAA 5 cut(s) 9, 318, 525, 1236, 1386
Tru9I TTAA 5 cut(s) 9, 318, 525, 1236, 1386
TscAI CASTG 1 cut(s) 1428
TseFI GTSAC 2 cut(s) 19, 945
TseI GCWGC 4 cut(s) 434, 772, 962, 1093
Tsp45I GTSAC 2 cut(s) 19, 945
TspDTI ATGAA 9 cut(s) 138, 249, 346, 614, 893, 1024, 1407, 1700, 1704
TspGWI ACGGA 3 cut(s) 105, 463, 806
TspRI CASTG 1 cut(s) 1428
Tth111I GACNNNGTC 1 cut(s) 1061
Van91I CCANNNNNTGG 1 cut(s) 97
VneI GTGCAC 1 cut(s) 1419
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 2 cut(s) 967, 1615
XceI RCATGY 3 cut(s) 155, 1192, 1410
XmnI GAANNNNTTC 1 cut(s) 935
XspI CTAG 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.