Rh4BG133200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008183
Physical Location & Seq
Forward (+)
22410541 .. 22411357
817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG133200.1

Sequence Viewer

Length: 723 bp
ATGTACGGATGGGTGGACAAGACTGCAAGACCATTGTGTGAATGGGGAGATGATCAAGTATTTAAACTGTTAAAGTTGGTGGAGGATCACGGAGGGGTATGCTCTTCTAACCACGAAGTTTGGGTACCAACCAAGGTATCCAGGAGAATGATATATGGAGCGTATGCTGCTGAAAGGGAAGGGACCGATAAGTGGGAAAGAGGCGGGGAGGTGATGACAGTTGAGAAGGAAGTTGAACAGTTGAAAATGGGTTTTGTTAACCTGGAGGAAGCTGTTGTTGTTATAATGCCAGAGATAGCTACCATGAAGGAAGCCGTTGAGAGGATGAGTAGGTTGGTCGGTGTGCACGATAGGGAGAGAATTGAACACATTGTATCACAGGTGGTGAGTCGACTAAATGACAGAGGTGGAAATAAGTTTGATGAGGAAAAAAACAAAAACAGAAACATTTGGACCTCACAGAAACGTGATAAAGGTGTAGACTCAACACAGTTCAATTATGGAAGTGAACGCGAGGAAGGTTCGGAGAGTTACTCATTGAAGTTCCTATATGTGAACAAAGGCGAACGTCGGGATGGGACAAGAAGGCAGCCGCATGATCTCTGTACGGAAGGCAAGGGAGAGCCGCAGGTTTCAGATCGATCAGCAGTATCAGTAAGCCCGACAGGGCCTCCCAAGGCAGCGCATAGAAGGACGAAAGAAAAAACTCGGAGGCCGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.51

Weight (kDa)

8.65

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 619
Acc65I GGTACC 1 cut(s) 124
AccB1I GGYRCC 1 cut(s) 124
AccI GTMKAC 2 cut(s) 391, 480
AccII CGCG 1 cut(s) 513
AciI CCGC 3 cut(s) 204, 593, 626
AclWI GGATC 1 cut(s) 93
AfaI GTAC 3 cut(s) 5, 126, 607
AfiI CCNNNNNNNGG 2 cut(s) 192, 321
AgsI TTSAA 5 cut(s) 236, 244, 365, 496, 541
AjnI CCWGG 2 cut(s) 140, 261
AluBI AGCT 2 cut(s) 272, 299
AluI AGCT 2 cut(s) 272, 299
Alw21I GWGCWC 1 cut(s) 348
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 1 cut(s) 93
AoxI GGCC 2 cut(s) 668, 713
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 167, 589, 680
Asp718I GGTACC 1 cut(s) 124
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 3 cut(s) 183, 453, 668
AsuHPI GGTGA 2 cut(s) 223, 397
AvaII GGWCC 2 cut(s) 183, 453
BaeGI GKGCMC 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BanI GGYRCC 1 cut(s) 124
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 154, 601, 692
BccI CCATC 2 cut(s) 3, 569
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 2 cut(s) 142, 263
BciVI GTATCC 1 cut(s) 148
BclI TGATCA 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 148
BisI GCNGC 5 cut(s) 168, 590, 593, 626, 681
BlsI GCNGC 5 cut(s) 169, 591, 594, 627, 682
Bme1390I CCNGG 2 cut(s) 142, 263
Bme18I GGWCC 2 cut(s) 183, 453
BmgT120I GGNCC 3 cut(s) 183, 453, 668
BmiI GGNNCC 2 cut(s) 126, 184
BmrFI CCNGG 2 cut(s) 142, 263
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 1 cut(s) 284
Bsa29I ATCGAT 1 cut(s) 640
BsaJI CCNNGG 2 cut(s) 132, 675
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 321
BseBI CCWGG 2 cut(s) 142, 263
BseCI ATCGAT 1 cut(s) 640
BseDI CCNNGG 2 cut(s) 132, 675
BseGI GGATG 3 cut(s) 14, 330, 580
BseLI CCNNNNNNNGG 2 cut(s) 192, 321
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 3 cut(s) 154, 601, 692
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 670, 715
BshNI GGYRCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 640
BsiHKAI GWGCWC 1 cut(s) 348
BslFI GGGAC 2 cut(s) 196, 592
BslI CCNNNNNNNGG 2 cut(s) 192, 321
BsmFI GGGAC 2 cut(s) 196, 592
BsnI GGCC 2 cut(s) 670, 715
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 5 cut(s) 52, 85, 598, 637, 641
BspACI CCGC 3 cut(s) 204, 593, 626
BspANI GGCC 2 cut(s) 670, 715
BspDI ATCGAT 1 cut(s) 640
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 2 cut(s) 126, 184
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 1 cut(s) 93
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 132, 675
BssMI GATC 5 cut(s) 52, 85, 598, 637, 641
BssT1I CCWWGG 2 cut(s) 132, 675
Bst2UI CCWGG 2 cut(s) 142, 263
Bst4CI ACNGT 4 cut(s) 69, 220, 240, 492
Bst6I CTCTTC 1 cut(s) 109
BstF5I GGATG 3 cut(s) 14, 330, 580
BstFNI CGCG 1 cut(s) 513
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 5 cut(s) 55, 88, 601, 640, 644
BstMBI GATC 5 cut(s) 52, 85, 598, 637, 641
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 2 cut(s) 142, 263
BstSCI CCNGG 2 cut(s) 140, 261
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 3 cut(s) 154, 601, 692
Bsu15I ATCGAT 1 cut(s) 640
BsuI GTATCC 1 cut(s) 148
BsuRI GGCC 2 cut(s) 670, 715
BsuTUI ATCGAT 1 cut(s) 640
BtsCI GGATG 3 cut(s) 14, 330, 580
BveI ACCTGC 1 cut(s) 619
CfoI GCGC 1 cut(s) 685
Cfr13I GGNCC 3 cut(s) 183, 453, 668
ClaI ATCGAT 1 cut(s) 640
Csp6I GTAC 3 cut(s) 4, 125, 606
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 2 cut(s) 304, 596
CviJI RGCY 8 cut(s) 272, 299, 314, 592, 625, 660, 670, 715
CviKI_1 RGCY 8 cut(s) 272, 299, 314, 592, 625, 660, 670, 715
CviQI GTAC 3 cut(s) 4, 125, 606
DpnI GATC 5 cut(s) 54, 87, 600, 639, 643
DpnII GATC 5 cut(s) 52, 85, 598, 637, 641
DraI TTTAAA 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 132, 675
Eco47I GGWCC 2 cut(s) 183, 453
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 140, 261
EcoT14I CCWWGG 2 cut(s) 132, 675
ErhI CCWWGG 2 cut(s) 132, 675
FaeI CATG 2 cut(s) 307, 599
FaqI GGGAC 2 cut(s) 196, 592
FatI CATG 2 cut(s) 303, 595
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 2 cut(s) 391, 480
Fnu4HI GCNGC 5 cut(s) 168, 590, 593, 626, 681
FokI GGATG 3 cut(s) 21, 337, 587
Fsp4HI GCNGC 5 cut(s) 168, 590, 593, 626, 681
GlaI GCGC 1 cut(s) 684
GluI GCNGC 5 cut(s) 168, 590, 593, 626, 681
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 670, 715
HhaI GCGC 1 cut(s) 685
Hin1II CATG 2 cut(s) 307, 599
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 2 cut(s) 259, 392
HindII GTYRAC 2 cut(s) 259, 392
HinfI GANTC 2 cut(s) 388, 482
HpaI GTTAAC 1 cut(s) 259
HphI GGTGA 2 cut(s) 223, 397
Hpy166II GTNNAC 7 cut(s) 16, 259, 346, 392, 481, 509, 556
Hpy188I TCNGA 3 cut(s) 526, 637, 711
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 7 cut(s) 16, 259, 346, 392, 481, 509, 556
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 7 cut(s) 173, 220, 301, 512, 579, 605, 684
HpyCH4III ACNGT 4 cut(s) 69, 220, 240, 492
HpyCH4IV ACGT 2 cut(s) 466, 568
HpyCH4V TGCA 2 cut(s) 26, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpySE526I ACGT 2 cut(s) 466, 568
Hsp92II CATG 2 cut(s) 307, 599
HspAI GCGC 1 cut(s) 683
KpnI GGTACC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 5 cut(s) 52, 85, 598, 637, 641
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 8 cut(s) 127, 154, 248, 275, 303, 365, 614, 651
Lsp1109I GCAGC 3 cut(s) 154, 601, 692
MaeII ACGT 2 cut(s) 466, 568
MaeIII GTNAC 1 cut(s) 530
MalI GATC 5 cut(s) 54, 87, 600, 639, 643
MboI GATC 5 cut(s) 52, 85, 598, 637, 641
MboII GAAGA 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 2 cut(s) 360, 496
MlyI GAGTC 2 cut(s) 397, 476
MseI TTAA 3 cut(s) 63, 71, 258
MspR9I CCNGG 2 cut(s) 142, 263
MvaI CCWGG 2 cut(s) 142, 263
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 5 cut(s) 52, 85, 598, 637, 641
NlaIII CATG 2 cut(s) 307, 599
NlaIV GGNNCC 2 cut(s) 126, 184
PciSI GCTCTTC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 5 cut(s) 169, 591, 594, 627, 682
PleI GAGTC 2 cut(s) 396, 476
PpsI GAGTC 2 cut(s) 396, 476
PsiI TTATAA 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 140, 261
PspGI CCWGG 2 cut(s) 140, 261
PspN4I GGNNCC 2 cut(s) 126, 184
PspPI GGNCC 3 cut(s) 183, 453, 668
RsaI GTAC 3 cut(s) 5, 126, 607
RsaNI GTAC 3 cut(s) 4, 125, 606
SalI GTCGAC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 63, 71, 258
SatI GCNGC 5 cut(s) 168, 590, 593, 626, 681
Sau3AI GATC 5 cut(s) 52, 85, 598, 637, 641
Sau96I GGNCC 3 cut(s) 183, 453, 668
SchI GAGTC 2 cut(s) 397, 476
ScrFI CCNGG 2 cut(s) 142, 263
SduI GDGCHC 1 cut(s) 348
SinI GGWCC 2 cut(s) 183, 453
Sse9I AATT 2 cut(s) 360, 496
SsiI CCGC 3 cut(s) 204, 593, 626
StyD4I CCNGG 2 cut(s) 140, 261
StyI CCWWGG 2 cut(s) 132, 675
TaaI ACNGT 4 cut(s) 69, 220, 240, 492
TaiI ACGT 2 cut(s) 469, 571
TaqI TCGA 2 cut(s) 391, 640
TaqII GACCGA 1 cut(s) 200
TasI AATT 2 cut(s) 360, 496
TauI GCSGC 2 cut(s) 595, 628
Tru1I TTAA 3 cut(s) 63, 71, 258
Tru9I TTAA 3 cut(s) 63, 71, 258
TseI GCWGC 3 cut(s) 167, 589, 680
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 3 cut(s) 21, 105, 623
VneI GTGCAC 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 183, 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 391, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.