Rh2CG387500

Belongs to the uridine kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003508
Physical Location & Seq
Forward (+)
52859359 .. 52863576
4218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG387500.1

Sequence Viewer

Length: 1281 bp
ATGGAGAGAGGCTCCGGGCCCCACTTGTGGGGGCTCCGACTCAACGGCCTCTTATCTTCTTCGCATTCATCACCTTCGCCTCTTTCGCTTGGCTCTTCTGCCGCCGCCGCCGACCTACACGACTCCAACGCCGCCAAGCAGCCCTTCGTCATCGGTGTTTCTGGAGTCGTTCTTGTCAATCAGGATTCATTTTACCGTGGTTTGACACCTGAGGAGTCTGAACGAGTCCATGAGTATAATTTTGATCATACTTATGCATTTGACACCGAACAGATGTTAGAATGTGTTCAAAGGCTGAAGTGCGGGCAGTCTGTCCATGTTCCAATTTATGATTTTAAGATACATCGACGCTGTTCTGATACTTTTCGCCAAGTGAATGCTTCTGATGTCATCATCTTGGAGGGAATTCTGATCTTTGTCGACACAGATGCTGATGTCAGACTTGCTCGTAGAATAAGACGAGACACTGTTGAGGGGGGCAGGGATATTGACTCTGTTCTTGAGCAGTATGCCAAGTTTGTCAAGCCTGCTTTTGATGATTTTGTTCTCCCCTCAAAGAAGTACGCTGATGTGATCATTCCCCGGGGAGGCGATAACCATGTTGCTATGGATTTAATTGTACAACATATTCAGACAAAGCTTGGTCAGCATGATCTTTGCAAAATATATCCTAATGTATACGTTATACAGTCCACATTTCAGATTAGAGGCATGCATACACTAATTCGAGACCGAGAAATATCAAAGCATGATTTTGTATTTTATTCGGATTGCCTAATTCGTCTGGTTGTGGAGCATGGCCTTGGTCATTTGCCATTCACAGAGAAACAAGTAATAACTCCACCAGGATCTGTGTATACTGGGGTTGATTTCTGCAAGAAATTATGTGGAGTTTCAATTGTTCGAAGTGGTGAGAGCATGGAAAATGCATTACGTGCATGTTGCAAGGGAATAAAAATTGGGAAGATCTTGATTCATCGTGAGCGAGACAATGGCAAACAGGATATTTCAGAGCGTCATGTCCTGCTTCTAGACCCTGTACTTGCTACAGGTAACCCTGCTAACCAGGCTATTGAACTACTCATACAGAAAGGGGTTCCTGAATCGCATATAATATTCTTAAACCTTATATCTGCTCCTGAAGGAATTCATTGTGTTTACAAACGATTTCCATCCTTGAAGATCGTCACTTCGGAGATTGATGTTGCCCTCAATGAAGAATTTCGTGTCATACCAGGGTTGGGTGAATTTGGTGATCGTTACTTTGGCACGGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004845 GO:0004849 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006109 GO:0006139 GO:0006206 GO:0006207 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009889 GO:0009987 GO:0010138 GO:0010556 GO:0010675 GO:0010962 GO:0016301 GO:0016310 GO:0016740 GO:0016757 GO:0016763 GO:0016772 GO:0018130 GO:0019205 GO:0019206 GO:0019222 GO:0019438 GO:0019637 GO:0019693 GO:0019856 GO:0031323 GO:0031326 GO:0032262 GO:0032881 GO:0032885 GO:0032950 GO:0032951 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0043094 GO:0043097 GO:0043173 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043455 GO:0044206 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046049 GO:0046112 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0055086 GO:0060255 GO:0065007 GO:0071704 GO:0072527 GO:0072528 GO:0080090 GO:0090407 GO:1900376 GO:1901135 GO:1901137 GO:1901141 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:2000112 GO:2000762 GO:2000904 GO:2001006
KEGG Pathways
Metabolic & Signaling
ko00030 ko00040 ko00240 ko00710 ko00983 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map00040 map00240 map00710 map00983 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PRK UPRTase

Protein Analysis

426

Amino Acids

47.66

Weight (kDa)

6.14

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRK PF00485 50 - 201 1.5e-29 Phosphoribulokinase / Uridine kinase family
UPRTase PF14681 231 - 424 1.8e-65 Uracil phosphoribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 420, 678, 857
AciI CCGC 5 cut(s) 102, 105, 108, 132, 303
AclWI GGATC 1 cut(s) 856
AcsI RAATTY 4 cut(s) 405, 1146, 1220, 1247
AcuI CTGAAG 2 cut(s) 317, 1161
AfaI GTAC 3 cut(s) 563, 621, 1041
AfiI CCNNNNNNNGG 4 cut(s) 27, 28, 587, 1241
AgsI TTSAA 4 cut(s) 290, 897, 1076, 1180
AjnI CCWGG 3 cut(s) 844, 1065, 1234
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 1 cut(s) 640
AluI AGCT 1 cut(s) 640
Alw26I GTCTC 3 cut(s) 456, 723, 981
AlwI GGATC 1 cut(s) 856
AlwNI CAGNNNCTG 2 cut(s) 431, 851
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 3 cut(s) 17, 46, 799
ApaI GGGCCC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 4 cut(s) 405, 1146, 1220, 1247
Asp700I GAANNNNTTC 3 cut(s) 285, 1146, 1221
AspS9I GGNCC 2 cut(s) 17, 18
AsuC2I CCSGG 3 cut(s) 16, 583, 584
AsuHPI GGTGA 4 cut(s) 63, 923, 1256, 1265
AsuII TTCGAA 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 582
AxyI CCTNAGG 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 21
BanII GRGCYC 2 cut(s) 21, 36
BbvI GCAGC 1 cut(s) 151
BccI CCATC 1 cut(s) 1180
BceAI ACGGC 1 cut(s) 61
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 3 cut(s) 846, 1067, 1236
BclI TGATCA 2 cut(s) 244, 573
BcnI CCSGG 3 cut(s) 16, 583, 584
BcoDI GTCTC 3 cut(s) 456, 723, 981
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 1 cut(s) 1047
BglII AGATCT 1 cut(s) 966
BisI GCNGC 5 cut(s) 102, 105, 108, 132, 140
BlsI GCNGC 5 cut(s) 103, 106, 109, 133, 141
Bme1390I CCNGG 6 cut(s) 16, 583, 584, 846, 1067, 1236
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 2 cut(s) 17, 18
BmiI GGNNCC 5 cut(s) 13, 19, 20, 35, 1098
BmrFI CCNGG 6 cut(s) 16, 583, 584, 846, 1067, 1236
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 870
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 904
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 3 cut(s) 16, 583, 584
BsaAI YACGTR 1 cut(s) 935
BsaBI GATNNNNATC 1 cut(s) 1171
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 6 cut(s) 196, 581, 582, 583, 802, 1235
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 28, 587, 1241
Bse1I ACTGG 1 cut(s) 865
Bse21I CCTNAGG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 1171
BseBI CCWGG 3 cut(s) 846, 1067, 1236
BseDI CCNNGG 6 cut(s) 196, 581, 582, 583, 802, 1235
BseGI GGATG 2 cut(s) 1172, 1279
BseJI GATNNNNATC 1 cut(s) 1171
BseLI CCNNNNNNNGG 4 cut(s) 27, 28, 587, 1241
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 865
BseRI GAGGAG 1 cut(s) 227
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 1 cut(s) 151
BshFI GGCC 3 cut(s) 19, 48, 801
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 2 cut(s) 15, 583
BslI CCNNNNNNNGG 4 cut(s) 27, 28, 587, 1241
BsmAI GTCTC 3 cut(s) 456, 723, 981
BsmI GAATGC 2 cut(s) 64, 382
BsnI GGCC 3 cut(s) 19, 48, 801
Bso31I GGTCTC 1 cut(s) 723
BsoBI CYCGRG 1 cut(s) 582
Bsp119I TTCGAA 1 cut(s) 904
Bsp120I GGGCCC 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 21, 36
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
BspACI CCGC 5 cut(s) 102, 105, 108, 132, 303
BspANI GGCC 3 cut(s) 19, 48, 801
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 5 cut(s) 13, 19, 20, 35, 1098
BspPI GGATC 1 cut(s) 856
BspQI GCTCTTC 1 cut(s) 100
BspT104I TTCGAA 1 cut(s) 904
BspTNI GGTCTC 1 cut(s) 723
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 1 cut(s) 865
BssECI CCNNGG 6 cut(s) 196, 581, 582, 583, 802, 1235
BssMI GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
BssNAI GTATAC 2 cut(s) 679, 858
BssT1I CCWWGG 1 cut(s) 802
Bst1107I GTATAC 2 cut(s) 679, 858
Bst2UI CCWGG 3 cut(s) 846, 1067, 1236
Bst4CI ACNGT 3 cut(s) 197, 469, 690
Bst6I CTCTTC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 935
BstAUI TGTACA 1 cut(s) 619
BstBAI YACGTR 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 904
BstC8I GCNNGC 3 cut(s) 305, 528, 713
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 1052
BstF5I GGATG 2 cut(s) 1172, 1279
BstKTI GATC 8 cut(s) 247, 414, 576, 655, 851, 969, 1185, 1258
BstMAI GTCTC 3 cut(s) 456, 723, 981
BstMBI GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
BstMWI GCNNNNNNNGC 5 cut(s) 85, 107, 646, 935, 1067
BstNI CCWGG 3 cut(s) 846, 1067, 1236
BstNSI RCATGY 2 cut(s) 715, 942
BstPI GGTNACC 1 cut(s) 1052
BstSCI CCNGG 6 cut(s) 14, 581, 582, 844, 1065, 1234
BstSFI CTRYAG 1 cut(s) 1047
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 848, 966
BstYI RGATCY 2 cut(s) 848, 966
BstZ17I GTATAC 2 cut(s) 679, 858
Bsu36I CCTNAGG 1 cut(s) 210
BsuRI GGCC 3 cut(s) 19, 48, 801
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 2 cut(s) 1172, 1279
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 3 cut(s) 305, 528, 713
CaiI CAGNNNCTG 2 cut(s) 431, 851
Cfr13I GGNCC 2 cut(s) 17, 18
Cfr9I CCCGGG 1 cut(s) 582
CseI GACGC 2 cut(s) 357, 1004
Csp6I GTAC 3 cut(s) 562, 620, 1040
CviQI GTAC 3 cut(s) 562, 620, 1040
DdeI CTNAG 1 cut(s) 210
DpnI GATC 8 cut(s) 246, 413, 575, 654, 850, 968, 1184, 1257
DpnII GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 802
Eco24I GRGCYC 2 cut(s) 21, 36
Eco31I GGTCTC 1 cut(s) 723
Eco57I CTGAAG 2 cut(s) 317, 1161
Eco81I CCTNAGG 1 cut(s) 210
Eco88I CYCGRG 1 cut(s) 582
Eco91I GGTNACC 1 cut(s) 1052
EcoO109I RGGNCCY 1 cut(s) 18
EcoO65I GGTNACC 1 cut(s) 1052
EcoRI GAATTC 2 cut(s) 405, 1146
EcoRII CCWGG 3 cut(s) 844, 1065, 1234
EcoT14I CCWWGG 1 cut(s) 802
EcoT22I ATGCAT 3 cut(s) 259, 717, 931
EcoT38I GRGCYC 2 cut(s) 21, 36
ErhI CCWWGG 1 cut(s) 802
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FauI CCCGC 1 cut(s) 296
FbaI TGATCA 2 cut(s) 244, 573
FblI GTMKAC 3 cut(s) 420, 678, 857
Fnu4HI GCNGC 5 cut(s) 102, 105, 108, 132, 140
FokI GGATG 1 cut(s) 1159
FriOI GRGCYC 2 cut(s) 21, 36
Fsp4HI GCNGC 5 cut(s) 102, 105, 108, 132, 140
FspBI CTAG 1 cut(s) 1031
GluI GCNGC 5 cut(s) 102, 105, 108, 132, 140
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 3 cut(s) 19, 48, 801
HapII CCGG 2 cut(s) 15, 583
HgaI GACGC 2 cut(s) 357, 1004
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 9 cut(s) 39, 122, 165, 185, 215, 225, 491, 973, 1103
HpaII CCGG 2 cut(s) 15, 583
HphI GGTGA 4 cut(s) 63, 923, 1256, 1265
Hpy166II GTNNAC 5 cut(s) 421, 679, 693, 858, 1159
Hpy188III TCNNGA 9 cut(s) 162, 182, 500, 728, 970, 980, 1031, 1100, 1139
Hpy8I GTNNAC 5 cut(s) 421, 679, 693, 858, 1159
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 3 cut(s) 84, 154, 1136
HpyCH4III ACNGT 3 cut(s) 197, 469, 690
HpyCH4IV ACGT 2 cut(s) 681, 934
HpyCH4V TGCA 7 cut(s) 257, 660, 715, 876, 929, 938, 945
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 107, 646, 935, 1067
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 681, 934
Ksp22I TGATCA 2 cut(s) 244, 573
Kzo9I GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 4 cut(s) 17, 39, 793, 1141
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 2 cut(s) 681, 934
MaeIII GTNAC 3 cut(s) 1052, 1186, 1259
MalI GATC 8 cut(s) 246, 413, 575, 654, 850, 968, 1184, 1257
MboI GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
MboII GAAGA 6 cut(s) 48, 51, 87, 976, 1192, 1229
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 848, 966
MhlI GDGCHC 2 cut(s) 21, 36
MlyI GAGTC 6 cut(s) 33, 116, 174, 224, 234, 485
MmeI TCCRAC 2 cut(s) 61, 150
MnlI CCTC 9 cut(s) 59, 90, 205, 394, 466, 562, 581, 701, 1220
Mph1103I ATGCAT 3 cut(s) 259, 717, 931
MroXI GAANNNNTTC 3 cut(s) 285, 1146, 1221
MseI TTAA 3 cut(s) 336, 614, 1121
MslI CAYNNNNRTG 1 cut(s) 252
MspI CCGG 2 cut(s) 15, 583
MspR9I CCNGG 6 cut(s) 16, 583, 584, 846, 1067, 1236
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 2 cut(s) 64, 382
MvaI CCWGG 3 cut(s) 846, 1067, 1236
MwoI GCNNNNNNNGC 5 cut(s) 85, 107, 646, 935, 1067
NciI CCSGG 3 cut(s) 16, 583, 584
NdeII GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
NlaIV GGNNCC 5 cut(s) 13, 19, 20, 35, 1098
NmuCI GTSAC 1 cut(s) 1186
NsiI ATGCAT 3 cut(s) 259, 717, 931
NspI RCATGY 2 cut(s) 715, 942
NspV TTCGAA 1 cut(s) 904
PaeI GCATGC 1 cut(s) 715
PciSI GCTCTTC 1 cut(s) 100
PctI GAATGC 2 cut(s) 64, 382
PdmI GAANNNNTTC 3 cut(s) 285, 1146, 1221
PfeI GAWTC 3 cut(s) 185, 973, 1103
PkrI GCNGC 5 cut(s) 103, 106, 109, 133, 141
PleI GAGTC 6 cut(s) 33, 116, 173, 223, 233, 485
PpsI GAGTC 6 cut(s) 33, 116, 173, 223, 233, 485
Ppu21I YACGTR 1 cut(s) 935
Psp6I CCWGG 3 cut(s) 844, 1065, 1234
PspEI GGTNACC 1 cut(s) 1052
PspGI CCWGG 3 cut(s) 844, 1065, 1234
PspN4I GGNNCC 5 cut(s) 13, 19, 20, 35, 1098
PspOMI GGGCCC 1 cut(s) 17
PspPI GGNCC 2 cut(s) 17, 18
PsrI GAACNNNNNNTAC 2 cut(s) 1068, 1100
PstNI CAGNNNCTG 2 cut(s) 431, 851
PsuI RGATCY 2 cut(s) 848, 966
RsaI GTAC 3 cut(s) 563, 621, 1041
RsaNI GTAC 3 cut(s) 562, 620, 1040
RseI CAYNNNNRTG 1 cut(s) 252
SalI GTCGAC 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 100
SaqAI TTAA 3 cut(s) 336, 614, 1121
SatI GCNGC 5 cut(s) 102, 105, 108, 132, 140
Sau3AI GATC 8 cut(s) 244, 411, 573, 652, 848, 966, 1182, 1255
Sau96I GGNCC 2 cut(s) 17, 18
SchI GAGTC 6 cut(s) 33, 116, 174, 224, 234, 485
ScrFI CCNGG 6 cut(s) 16, 583, 584, 846, 1067, 1236
SduI GDGCHC 2 cut(s) 21, 36
SetI ASST 8 cut(s) 76, 117, 211, 642, 684, 937, 1054, 1128
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 1047
SfuI TTCGAA 1 cut(s) 904
SmaI CCCGGG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 1 cut(s) 715
SsiI CCGC 5 cut(s) 102, 105, 108, 132, 303
SspI AATATT 1 cut(s) 1116
SspMI CTAG 1 cut(s) 1031
StyD4I CCNGG 6 cut(s) 14, 581, 582, 844, 1065, 1234
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 3 cut(s) 197, 469, 690
TaiI ACGT 2 cut(s) 684, 937
TaqI TCGA 4 cut(s) 346, 420, 727, 904
TaqII GACCGA 1 cut(s) 747
TatI WGTACW 2 cut(s) 619, 1039
TauI GCSGC 4 cut(s) 104, 107, 110, 134
TfiI GAWTC 3 cut(s) 185, 973, 1103
Tru1I TTAA 3 cut(s) 336, 614, 1121
Tru9I TTAA 3 cut(s) 336, 614, 1121
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 1 cut(s) 1186
TseI GCWGC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 1186
TspDTI ATGAA 5 cut(s) 57, 177, 965, 1139, 1230
TspMI CCCGGG 1 cut(s) 582
TspRI CASTG 1 cut(s) 472
XapI RAATTY 4 cut(s) 405, 1146, 1220, 1247
XbaI TCTAGA 1 cut(s) 1030
XceI RCATGY 2 cut(s) 715, 942
XmaI CCCGGG 1 cut(s) 582
XmiI GTMKAC 3 cut(s) 420, 678, 857
XmnI GAANNNNTTC 3 cut(s) 285, 1146, 1221
XspI CTAG 1 cut(s) 1031
Zsp2I ATGCAT 3 cut(s) 259, 717, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.